bibliography of in vitro in vivo correlations

... analogues in their affinity for binding proteins In view of this, an important practical consequence of our simulations results are that the testing of newly designed hormones in in vivo systems ... M, Franks S: Diurnal variation of sex hormone binding globulin and insulin-like growth factor binding protein-1 in women with polycystic ovary syndrome Clin Endocrinol (Oxf) 1995, 43:159-165 Publish ... LM, Mercado M, Baumann G: Influence of high affinity growth hormone binding protein on plasma profiles of free and bound GH and on the apparent half-life of GH J Clin Invest 1993, 91:629-641 Baumann...

Ngày tải lên: 13/08/2014, 22:22

... the inguinal lymph node of the mouse by folding back a broad flap of abdominal skin or the popliteal lymph nodes of the feet [25] A rubber ring is glued on the inner surface of the exposed skin ... capacity of DCs and thus certainly influence the way in which they interact with T cells Future directions and challenges for imaging in vivo Future improvements of cell tracking techniques in vivo ... Nevertheless, the combined employment of various imaging techniques together with the right kind of accompanying studies in vitro will certainly provide a deepening understanding of the complex cellular...

Ngày tải lên: 09/08/2014, 07:20

... obtained during the clinical test that yielded failure of the bone-implant interface was implemented in definition of loading conditions in the finite element analysis In the definition of loading condition, ... properties of the interface, the purpose of this biomechanical study was to quantify failure torque of an osseointegrated implant with severe bone loss and involve the in vivo data in finite element ... surface of the abutment was selected and retained All other nodes resting on the occlusal surface and their degree of freedom (dof) were connected to centrally retained node using RBE link (MSC.Software...

Ngày tải lên: 11/08/2014, 23:22

... changed near EGR-1 binding sites, suggesting that EGR-1 binding influences the occupancy of previous binding proteins These observations may help explain why EGR-1 binding results in multiple responses ... SP1 binding occupancies change dramatically EGR-1 binding may influence the occupancy of previous binding proteins, resulting in the reconstruction of the transcription factor complex and the induction ... binding 3.08E-019 GO:0003723 RNA binding 6.44E-018 GO:0005515 Protein binding 5.65E-009 GO:0000166 Nucleotide binding 3.84E-007 GO:0003677 DNA binding 5.69E-007 GO:0051082 Unfolded protein binding...

Ngày tải lên: 14/08/2014, 21:20

... function of the increasingly large group of proteins that have been shown to contain intrinsically unstructured regions The intrinsic conformation flexibility that is inherent in this binding mechanism ... activator-binding domains is also of interest in relation to the twostep binding mechanism between Gal4 and Swi1 that has been characterized in vitro [34], and our results suggest that the coupled binding ... Error bars indicate the SD of means obtained from three independent cultures Swi1 or Snf5 activator-binding domains showed little, if any, reduction in the level of galacose induction of the same...

Ngày tải lên: 07/03/2014, 00:20

... checkpoint signaling in vivo It may function in the sensing of DNA damage [22] In contrast with the intensive study of RPA in animals and yeast, little is known about this protein in plants In 1997, ... experiment, the indicated proteins were incubated together in NaCl ⁄ Pi containing 250 mM NaCl at °C for h, and then incubated with Talon beads The amount of protein present in the fraction bound ... of OsRPA70a recombinant protein was found in the soluble fraction of OsRPA70a–OsRPA32–OsRPA14 extracts but most of it failed to bind to the GST column (Fig 4A), indicating that it could not interact...

Ngày tải lên: 07/03/2014, 21:20

... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding ... or light harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and...

Ngày tải lên: 07/03/2014, 21:20

... TOF Analysis of the in vivo cultured A salmonicida strain 80204-1 cells In vivo culture was performed using ligated dialysis tubing bags filled with bacterial suspension of A salmonicida strain ... determined following the hydrolysis of the carboxyl-reduced CPS Presence of L-alanine was confirmed by amino acid analysis For this, 0.6 mg of CPS was hydrolysed in 5.7 M hydrochloric acid containing ... ratios of 3-amino-3-deoxy-D-quinovose and L-rhamnose,  10% of the newly formed CPS and LPS O-chain polysaccharide was present in the in vivo cultured cells Methylation analysis performed on in vivo...

Ngày tải lên: 23/03/2014, 13:20

... pneumoniae SPase I For in vitro self-cleavage of S pneumoniae SPase I in the presence of phospholipid, 20 lL of reaction containing lg of purified SPase I was incubated in 37 °C for h in 20 mM Tris/HCl ... the protein The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23] In vitro self-cleavage was also observed in all investigated bacterial SPase I including enzymes ... cleavage of prestreptokinase, typically, reactions (20 lL) containing 0.1 lg SPase I or SPase37–204 were incubated with lg of purified prestreptokinase at 37 °C for h in the buffer containing 20...

Ngày tải lên: 23/03/2014, 21:21

... signal of binding of the fluorescent probe is given as extent of carbohydrate-ligand-dependent binding by subtracting carbohydrate-independent binding from total binding for each cell line BiB10-BSA ... avoided In the solid-phase assay, saturable and carbohydrate-dependent binding was measured, and the resulting Scatchard plots gave straight lines indicating the presence of a single class of binding ... neoglycoprotein and biotin-independent binding of the second-step reagent was subtracted from the results to determine the level of specific (glycan-dependent) binding Having confirmed the validity of the...

Ngày tải lên: 30/03/2014, 13:20

... from infected cells that were not treated with SCH 16 showed the presence of JEV proteins (Figure 4) In vivo evaluation of compounds against JEV using mouse model After ascertaining the in vivo ... http://www.virologyj.com/content/5/1/64 Figure of Kinetics action of SCH 16 in relation to the replicative cycle of JEV in PS cells Kinetics of action of SCH 16 in relation to the replicative cycle of JEV in PS cells.Panel ... 100% inhibition of viral plaques were evaluated by in vitro experiments detailed below Determining kinetics of JEV replication in PS cells A 24 well plate containing sterile cover slips in each...

Ngày tải lên: 20/06/2014, 01:20

... description of RNAi in mammalian cells proved that the RNA silencing machinery is conserved in mammals [10] In some cases, a strong antiviral effect of RNAi was observed in the cases of human immunodeficiency ... sequences without alterations, ensuring the effective activity of the siRNAs described in the present study This work offers an insight into the use of RNAi in animal breeding for disease resistance The ... with 50–100 ug of plasmids dissolved in 100 ul of saline Mice of the control group were subcutaneously injected with saline After h, the suckling mice were challenged with and 20 LD50 of the FMDV...

Ngày tải lên: 20/06/2014, 01:20

... Effects of active immunization of sheep against an amino terminal peptide of the inhibin αC subunit on intrafollicular levels of activin A, inhibin A and follistatin Journal of Endocrinology ... important in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol ... resulted in a final follicular fluid concentration of 50 μM of the inhibitors, and 50 μM (0.005%) of the DMSO Concentrations of the inhibitors were based on the treatments used in vitro in Experiment...

Ngày tải lên: 20/06/2014, 07:20

... (s.c.) injections in the flank with × 106 cells in 0.2 mL of media containing 20% Matrigel ™(Becton Dickinson, Bedford, MA, USA) or intraperitoneal (i.p.) injections of × 10 cells in mL of media ... of and coordinated the studies and assisted with the in vivo experiments All authors assisted in the drafting of the manuscript, the final version of which has been read and approved by all of ... analyzed using AMIDE (A Medical Image Data Examiner) software program To minimize spillover effects, regions of interest (ROIs) were drawn to enclose approximately 80-90% of the organ of interest,...

Ngày tải lên: 21/06/2014, 02:20

... were suspended in 100 μL of culture medium and subcutaneously inoculated into the right flank of mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study ... and development of multidrug resistance Human hepatoma HepG2 cells were purchased from the Institute of Hematology of Tianjin, Chinese Academy of Medical Sciences (Tianjin, China) To develop ... MDR1 levels of HepG2 and HepG2/ADM cells The drug-resistant HepG2/ADM cells were cultured in the cell culture medium containing μg/ mL adriamycin (Sigma) Both cell lines were maintained in RPMI-1640...

Ngày tải lên: 21/06/2014, 03:20

... provide rapid in vitro screens for analyzing new therapies and discovering means of achieving selective protection or targeted killing In light of the ease of use of these in vitro and in vivo assays, ... Immunofluorescence and TUNEL staining in vivo Free-floating sections (40 µm) were used for all in vivo experiments for TUNEL staining and combined immunofluorescence staining Detection of nuclear ... decreasing series of ethanol in 90%, 70%, and 50% for each, followed by rinsing in distilled water After three rinses in TBS, the sections were exposed to To combine TUNEL staining with immunofluorescence...

Ngày tải lên: 06/08/2014, 18:21

... rooting in vitro and subsequently of micropropagated plants in the field Similar increased rooting ability of cuttings from micropropagated stockplants of the plum rootstock Pixy has been sustained ... aL, 1989a) Differences in shoot production and rooting of M.9 in vitro also appeared to originate from differences between explants used to initiate culture lines Decreasing dependence with subculture ... Company for contributing to the funding of this work Rooting summer References Howard B.H., Jones O.P & Vasek J (1989a) Long-term improvement in the rooting of plum cuttings following apparent rejuvenation...

Ngày tải lên: 09/08/2014, 02:21

... cells in vitro was tested in vivo We administered amiloride in vivo and showed reduction in the magnitude of CII-specific, but not polyclonal, T cell responses in draining lymph nodes, suggesting ... denoting 95% confidence interval are presented effect of amiloride and cytochalasin D was independent of NFκB inhibition Dose-response data obtained in the absence of inhibitors presented in Figures ... of CII uptake into late endosomes in pulse-chase experiments in the presence of inhibitors of uptake Pretreatment of macrophages with cytochalasin D or amiloride reduced accumulation of CII in...

Ngày tải lên: 09/08/2014, 08:22

... fragment of DNA-PKcs DNA-PKcs is approximately 470 kD consisting of 4128 amino acids Its C-terminus contains a serine/threonine protein kinase domain (3719- 4128) [23] For screening Page of 11 the ... binding domain of DPK3-scFv, it is reasonable that DPK3-scFv can directly interfere the phosphorylations of DNA-PKcs, including site of S2056, which, in some extent, results in a deficient of ... similar inhibiting effect as the parental mAb on the kinase and DNA end joining activities of DNA-PKcs in cell-free systems Microinjection of purified scFv18-2 protein sensitized human skin melanoma...

Ngày tải lên: 09/08/2014, 09:20

... rating of "minimal," scores of to receive a vaginal irritation rating of "mild," scores of to 11 receive a vaginal irritation rating of "borderline," and scores of 12 to 16, receive a vaginal ... particles in vitro [25], caused lesions in the vaginal epithelium in vivo and increased the probability of being infected with HIV-1 [26] To assess whether biological activity of C14 causes inflammation ... lyophilization of the resulting product to obtain the pure final derivative in the form of a powder soluble in water Synthesis of zinc tetra-ascorbocamphorate derivative is around 10 cents of euro per...

Ngày tải lên: 10/08/2014, 05:21