assembly of malonyl and acetyl groups on acp
Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"
Ngày tải lên :
26/10/2012, 09:07
... regulation of enzyme production and increased clearance along with the modulation by NO The figure shows the concentration of NOx, TAS and TBARS in this study The concentration of NOx and TBARS ... down regulation of GS production and an increased clearance of this enzyme apart from modulation of GS by NO Activity of the GS could be involved in the regulation of concentration of glutamate ... versus control group; b p
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Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc
Ngày tải lên :
20/02/2014, 02:21
... quantitative contribution in vivo where the concentration of cysteine is low (15 lM), this reaction contributed signicantly to the production of pyruvate under our conditions, where the concentrations of ... same range of concentration (Fig 6) Time-constant of the branch-point system Fig Comparison of the kinetic eciencies of CGS and TS CGS and TS velocities as a function of Phser concentration v/[CGS] ... physiological conditions The partition was measured in the conditions of Fig 2B in the presence of 20 lM AdoMet and a physiological concentration of AMP (100 lM [34]) Under these conditions, we observed...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
Ngày tải lên :
21/02/2014, 15:20
... enthalpy of the reaction under the chosen experimental conditions, and [E] is the enzyme concentration The correction for the enzyme dilution and liquid displacement upon substrate injection was ... experimental conditions used for GTP activation on the basis of the kinetic constants in Table 1, and those obtained for the glutaminase reaction in the presence of 0.1 mM each of UTP and ATP-cS ... the degree of saturation with ATP and UTP, unlike ATP-cS and UTP, has a large inuence on Ka for GTP This correlation of a decrease in Ka for GTP with the lowering of the concentration of nucleotide...
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Báo cáo khoa học: Inactivating pentapeptide insertions in the fission yeast replication factor C subunit Rfc2 cluster near the ATP-binding site and arginine finger motif docx
Ngày tải lên :
07/03/2014, 02:20
... discussion, the mutations are considered in three groups dependent on their location in the 3D structure of the Rfc2 protein Mapping the insertions onto the structure of Rfc2: insertions into ... re-ligating, the bulk of the transposon is deleted, leaving behind only 15 bp of sequence derived from the ends of the transposon and the target site duplication Should Tn4430 insertion occur within ... K33 and K27 insertion sites were deleted (Table 1) Similar alleles were constructed using rfc2-K33 and rfc2-K22 (rfc2K33D22) and using rfc2-K23 and rfc2-K14 (rfc2K23D14) Note that construction of...
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Báo cáo khoa học: Two overlapping antiparallel genes encoding the iron regulator DmdR1 and the Adm proteins control sidephore and antibiotic biosynthesis in Streptomyces coelicolor A3(2) pdf
Ngày tải lên :
07/03/2014, 02:20
... behaviour, one of the clones for each of these two mutations was chosen at random for further experiments and named TAdmdR1 (strain in which translation was arrested in the sense strand of dmdR1) and ... lack of translation of one gene affected expression of the antiparallel one, western blot analyses of formation of the DmdR1 protein were performed for the various mutants with either deletion ... DmdR1 or deprivation of iron leads to deregulated (constitutive) formation of desferrioxamines However, expression of adm is required for overall function of the iron regulatory control, as the DdmdR1...
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Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx
Ngày tải lên :
07/03/2014, 21:20
... expression of GFP and the chloroplast autofluorescence, excitation lines of an argon ion laser of 488 nm were used with a 505 ⁄ 550-nm bandpass filter for GFP and excitation lines of an HeNe laser of ... the level of GFP expression by growing Clone 31 in medium containing mixtures of ammonium and nitrate At relative molar concentrations of nitrate vs ammonium of 50 (Q50) and 100 (Q100), GFP fluorescence ... the addition of both nitrate and appropriate concentrations of ammonium allows, within limits, the control of the amount of induced protein The observed lag phase of h for Pnr ⁄ Tnr-controlled...
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Báo cáo khoa học: Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III ppt
Ngày tải lên :
23/03/2014, 05:22
... buried upon dimerization is 2390 A2, and the putative dimerization interface mostly involves interactions between residues of the helical domain of one monomer and residues of the protease and apical ... interactions between Asn628-NAG2 of one monomer and the side chain of Glu266 of the apical domain of the second monomer The binuclear metal center Electron density maps reveal the presence of two ... existence of two alternate conformations of its side chain, stabilization of the Asp443 position as a result of a hydrogen bond to the Ser509 hydroxyl group might be weakened Decreased stabilization of...
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Báo cáo khoa học: Perturbation of folding and reassociation of lactate dehydrogenase by proline and trimethylamine oxide potx
Ngày tải lên :
23/03/2014, 15:21
... irrespective of the diffusion of the monomer (or dimer) per se The correlation between the effect of increasing proline concentration on viscosity and on reactivation of acid-dissociated enzyme (Figs and ... determinations; error bars are shown where the magnitude of the error exceeds the size of the symbol Fig Effect of proline concentration on the extent of reactivation of LDH from bovine heart and on the ... initiation of reactivation the activities of controls and the mixture containing M Ó FEBS 2003 Perturbation of protein folding by osmolytes (Eur J Biochem 270) 4827 Fig Effect of proline concentration on...
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Báo cáo khoa học: The N-acetylglutamate synthase/N-acetylglutamate kinase metabolon of Saccharomyces cerevisiae allows co-ordinated feedback regulation of the first two steps in arginine biosynthesis potx
Ngày tải lên :
23/03/2014, 21:20
... alignments of known members of the N-acetylglutamate kinase family illustrate conservation over all three domains of living organisms (Bacteria, Archaea, and Eucarya) of a region corresponding to ... fragments allow the expression of the ORF under the transcriptional control of the IPTG-inducible strong bacterial trp-lac promoter and under the translational control of an appropriate Shine–Dalgarno ... strains and growth conditions) The arginine deprivation results in a Gcn4pmediated transcriptional activation of the ARG2 gene (K Pauwels and M Crabeel, unpublished results) and reduces the pool of...
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Báo cáo y học: "Chlamydia trachomatis Infection of Human Trophoblast Alters Estrogen and Progesterone Biosynthesis: an insight into role of infection in pregnancy sequelae." doc
Ngày tải lên :
08/08/2014, 16:23
... infection of human trophoblast In order to evaluate the impact of infection of trophoblast on C trachomatis forms and status, we assessed the expression of the molecular determinants of chronicity ... emission wavelength of 485nm and an excitation wavelength of 530nm using CytoFluor 4000 ((Applied Biosciences, Woodinville, CA) Protein estimation [21] was also done on lysate Progesterone was ... the induction of trophoblast function during pregnancy depends on human chorionic gonadotropin by trophoblast, we decided to assess the effect of C trachomatis infection on hCG production by trophoblast...
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báo cáo khoa học: " Berry skin development in Norton grape: Distinct patterns of transcriptional regulation and flavonoid biosynthesis" docx
Ngày tải lên :
11/08/2014, 11:21
... Expression profiles of key genes and accumulation of anthocyanins and PAs display a good correlation in Norton berry skin A concise summary of coordinated transcription of key genes and biosynthesis ... flavonoid biosynthesis pathway and the accumulation profiles of flavonoid compounds Comparative analysis of key genes in flavonoid biosynthesis and of the main flavonoid compounds between Norton ... 81-100% Figure A concise representation of qPCR and HPLC data for visualizing the coordination of transcriptional regulation of the genes and the total amounts of anthocyanins and proanthocyanidins...
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Báo cáo y học: " Effects of carbohydrate, branched-chain amino acids, and arginine in recovery period on the subsequent performance in wrestlers" potx
Ngày tải lên :
11/08/2014, 23:21
... secretion [14, 15] The consumption of leucine and arginine along with glucose could result in higher insulinemic response compared to glucose alone in healthy subjects at rest [16] In addition, ... supplementation of CHO and CHO+AA resulted in significantly lower plasma concentrations of glycerol and NEFA at 90 and 120 after match 2, as well as immediately after match (Figures and 5) Plasma ... population and certain patients [53] Thus, arginine supplementation did not provide any additional effect on NO production in our subjects The lack of effect of carbohydrate supplementation, with...
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Manipulation of cyanogenesis and starch biosynthesis in cassava
Ngày tải lên :
16/09/2015, 17:11
... various stages of growth Effects of concentration of urea on gelatinization of starch granules from the cassava plants Effect of M urea on the swelling of starch granules of wild-type and mutant ... 1994) and fatal and nonfatal acute poisoning (Akintonwa et al., 1994) The onset of these disorders can be 22 gradual or immediate and is dependent on the prior state of health and nutrition of the ... parts of many crops and an understanding of regulation of its biosynthesis will aid to make directed changes in composition/quality Second, increase in human population and shrinking land and water...
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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc
Ngày tải lên :
18/02/2014, 18:20
... responsible for ammonium assimilation into glutamine and glutamate, as a result of a decline in GS2 and the induction of the a-GDH subunit (for a review, see [10]) However, the role of GDH is controversial ... Photosynthetic (s) and nonphotosynthetic (d) combinations contained L-FNR Fd I and R-FNR Fd III, respectively Oxidation of NADPH was followed by monitoring the decrease in A340 nm Table Comparison of Fd-GOGAT ... there was little contamination from xylem, and vice versa Glx (glutamine and glutamate: ve carbon amide and amino acid) and Asx (asparagine and aspartate: four carbon amide and amino acid) were...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
Ngày tải lên :
19/02/2014, 02:20
... transition state and hold E52 in the correct position to initiate the catalytic reaction The transition state of this type of kinases is considered to be close to trigonal bipyramidal geometry of ... Kirschbaum B & Loffler M (1996) Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified ... Relative phosphorylation expressed as a relation of the percentage of dThd phosphorylation of the corresponding mutant is given in parentheses The substrate concentration is 100 lM Experiments...
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Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx
Ngày tải lên :
07/03/2014, 04:20
... AGAT and arginase II In these tumor cells, SAH hydrolase and methionine synthase mRNA levels are high, indicating the activation of the pathway for the utilization of SAH and formation of methionine ... of polyamines and methionine An investigation of the level of different intermediate metabolites and the expression of different enzymes in the metabolic cycle of arginine and methionine further ... (aminopropylation), and functions as the sole methyl group donor in various other transmethylation reactions, as needed for creatine and glutathione synthesis (transulfuration) [34–36] Each transmethylation...
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Báo cáo lâm nghiệp: " The role of glutamine synthetase, glutamate synthase and glutamate dehydrogenase in ammonia assimilation by the mycorrhizal fungus Pisolithus tinctorius" ppt
Ngày tải lên :
09/08/2014, 04:20
... in the donation of nitrogen for the anabolism of nitrogenous metabolites Discussion and Conclusions Enzyme assays showed that the ECM fungus P tinctorius was capable of ammonia assimilation by ... GDH After h the glutamine concentration peaked and remained stable, indicating feedback control of GS Inhibition of aminotransferases (Fig 3d) led to an accumulation of glutamate after h, showing ... growth (5-10 d) and decreased thereafter with ammonium concentration (Fig 2a) Ammonia assimilation All extracts were found to contain significant amounts of arginine, which is thought to play a major...
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báo cáo khoa học: " Ornithine-δ-aminotransferase is essential for Arginine Catabolism but not for Proline Biosynthesis" ppsx
Ngày tải lên :
12/08/2014, 05:20
... provide experimental confirmation of the predicted localisation of δOAT in mitochondria using a δOAT-GFP fusion protein With the use of loss -of- function T-DNA insertion mutants we demonstrate that δOAT ... supporting the concept that Orn conversion can contribute to Pro accumulation [24,25] Use of gabaculine as a potent inhibitor of δOAT suggested that in radish cotyledons Orn conversion could contribute ... fusion construct under control of the CaMV 35S promoter A: Fluorescence signal of MitoTracker orange; B: GFP signal; C: Autofluorescence of chlorophyll; D: Merge of A and B; E: Merge of B and...
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Molecular biology of glutamate dehydrogenase and glutamine synthetase in two air breathing teleosts
Ngày tải lên :
13/10/2015, 15:54
... intestine and brain of M albus………………………………………… 3.2.2 Phylogeny and conservation of Gdh……………………… 3.2.3 mRNA expressions of gdh in the liver, intestine and brain of M albus exposed to terrestrial conditions ... downregulation of gs mRNA expression in the liver and intestine of M anguillicaudatus Furthermore, even though ammonia loading conditions led to an initial up-regulation of hepatic and intestinal ... rate of glutamate deamination by intact catfish liver mitochondria can account for 160% of the rate of ammonia excretion (Campbell et al., 1983) On the other hand, the rates of alanine and glutamine...
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