application of atomic force microscopy measurements on cardiovascular cells

... This amount corresponds to an average thickness of about nm This calculation gives a reasonable confirmation of the mass conservation law validity Concerning the formation of the small holes ... growth in two dimensions and considered the formation of depletion zones A depletion zone around a large cluster, originates from the shrunken smaller clusters Such depletion zones would have circular ... Furthermore, from the observation of the formation of depletion zones around the largest clusters and by the quantification of their time evolution, the Au surface diffusion coefficient Ds(T) = (7.42...

Ngày tải lên: 21/06/2014, 05:20

... sputtered Au films are more consistent with a conservative deposition process (i.e prediction of the SP equation) rather than a nonconservative one (i.e prediction of the KPZ equation) Other experiments ... equation for nonequilibrium and conservative systems yields a = and b = 0.25 for growth of a two-dimensional surface [8] The values of a and b predicted by the theories for nonconservatives and conservatives ... the sample with a thickness of nm b of nm, c of 14 nm, d of 20 nm, e of 26 nm, f of 32 nm of Au respectively The continuous lines represent the fit by Eq The values of ci reported as insets are...

Ngày tải lên: 22/06/2014, 01:20

... exist as an intermediate packing configuration Furthermore, we find that LH-2, in all cases, show a tilted conformation One of the two types of LH2 dimer configurations is shown to possess a tilt that ... dialysis [26] On the basis of AFM imaging of both the periplasmic and cytoplasmic sides of LH2’s after reconstitution, ellipticity and tilt of the LH2’s have been reported [14,21,23] A comparison between ... dimeric configuration Detailed investigations of the observed LH2 packing patterns reveal that the different packing lattices consist of similarly interacting LH2’s The dimeric LH2 configuration is...

Ngày tải lên: 18/02/2014, 18:20

... GNPs on the C-termini of the enzyme In contrast, the longer distance indicates a probable extension of the C-termini of V-PPase Verication of these possibilities requires further investigation ... respect to the concentration of V-PPase and reaction time under these conditions (data not shown) After incubation, the reaction was terminated with a stop solution [1.7% (w/v) ammonium molybdate, ... cross-section shown in (B) Two populations of V-PPase protrusions were observed: one with a mean height of 1.2 0.1 nm (n = 20), and one with a mean height of 2.8 0.3 nm (n = 17) (D) Histogram of V-PPase...

Ngày tải lên: 16/03/2014, 02:20

... (1995) Atomic- force microscopy of nucleoprotein complexes Scanning Microscopy 9, 705–727 Yang, J., Mou, J., Yuan, J.Y & Shao, Z (1996) The effect of deformation on the lateral resolution of atomic force ... sequences Conformational variability of triplex structures can provide tuning of the interaction of the triplexforming regions In addition, triplex formation absorbs the negative supercoils of the ... function in some processes of genome regulation such as replication rate and timing, recombination and chromosome folding by modulating the local structure of DNA regions and the global topology of...

Ngày tải lên: 31/03/2014, 23:20

... scratched at 10 μN of tip force, μm/s of speed (c) The cross-section profiles of the grooves at the position as indicated by the line (d) Schematic of oblique cutting, inclination angle θ defined ... cross-section area increases much faster than the depth Consequently, the nonlinear behavior of the relationship between groove dimension and tip force results from the nonlinear increments of the ... National Natural Science Foundation of China (nos 10874040, 90306010, and 20803018) and the Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China...

Ngày tải lên: 21/06/2014, 00:20

... contaminated at the first touch and continue with the following touches, and this contamination can influence the next interaction of the tip with the cells Therefore, contamination control of ... causes nuclear translocation of the nuclear factor (NF)-kappaB transcription factor The inhibition of NF-kappaB activation blocks maturation of DCs, followed by down-regulation of major histocompatibility ... morphologic change is the foundation of the phenotype and the function of BMDCs MHC-II is one of activation molecules expressed on the surface of BMDCs, representing a phenotype of mature BMDCs Flow cytometry...

Ngày tải lên: 21/06/2014, 02:20

... functionalization of CNTs with gold nanoparticles (AuNPs) is also investigated as a possible mean to improve their electrical conductivity Finally, the contiguous question of local modification of ... approach of the conduction mechanism with the diamond tip, we adapt our resistance model (see Figure 3b) by introducing the additional contribution of a Schottky diode between the R tip-CNT contact ... controlled by the Schottky barriers that forms the metal-CNT junction, the nature and geometry of this contact can strongly «Tip-CNT-substrate» junction analysis model The current conduction of...

Ngày tải lên: 21/06/2014, 04:20

... on nano-interfaces of semiconductors and organic materials towards opto-electronic and bio-electronic applications Main interests lie in characterization and modification of material, electronic, ... wear rate of FBS layer on O-diamond using medium k cantilevers as a function of oscillation amplitude A in solution and in air Energy of oscillation at specific amplitude is given on the top ... possibility of tailoring diamond surface properties by various atomic terminations, it may have even broader application for fundamental studies Page of 10 of molecules on surfaces in general Based on...

Ngày tải lên: 21/06/2014, 04:20

... characterization of Schottky diodes based on boron doped homoepitaxial diamond films by conducting probe atomic force microscopy Superlatt Microstruct 2006, 40:343 21 Lampert A, Mark P: Current Injection ... explanations are that the whole surface of the remaining piece of silicon in contact with the a-Si:H layer fully contributes to decrease the electrical contact resistance or that the friction of the ... Conducting probe atomic force microscopy applied to organic conducting blends Appl Phys Lett 2001, 79:2993 19 Alvarez J, Kleider JP, Houze F, Liao MY, Koide Y: Local photoconductivity on diamond...

Ngày tải lên: 21/06/2014, 06:20

... deviation Control of Functionality of Monomeric CYP11A1 These assays were aimed toward demonstrating the functionality of monomeric CYP11A1 For this purpose, we investigated the conversion of 7-dehydrocholesterol ... on particles in solution by use of photon correlation spectroscopy (PCS) Measurements were made at the light-scattering angle of 90° Protein solution (the stock one or the one subjected to monomerization ... height was built AFM of Non-Monomerized and Monomerized CYP11A1 AFM was used for visualization of non-monomerized and monomerized CYP11A1 The distribution densities r(h) of CYP11A1 images at...

Ngày tải lên: 21/06/2014, 11:20

... corresponding pulse durations of 50 ms In the present work, the presented data are an average of five measurements To investigate the conversion mechanisms of GaAsoxides, the chemical composition of ... ratio of oxide nanostructures The proper control of the oxidation reaction, improvement of reproducibility and increasing the accuracy are among the immediate objectives for further evolution of ... within the context of the anodic oxidation concepts, the anionic and cationic transport are important factors in determining the kinetics of oxidation In this scenario, the driving force is the...

Ngày tải lên: 22/06/2014, 01:20

... region c Power spectrum of the above profile showing the scaling of roughness d Histogram plot of the height of the head region e 3D image of the head region of the spermatozoa mechanical response ... region may often lead to the loss of functional competence of the spermatozoa The major advantage of AFM in pathological studies of spermatozoa is that it allows the evaluation of position and ... position of the viral particles located on the sperm membrane and their merging on the surface of spermatozoa were detected with high precision Unlike electron microscopy, and other conventional...

Ngày tải lên: 11/08/2014, 00:22

... shown) Consequently cross contaminations are negligible with this method Conclusions A novel approach has been presented for the high resolution immobilization of multiple biomolecules on a Page of ... contact mode with 3.4 mN contact force The tip remained in contact for seconds and changed to the next spotting positions by retraction Ink was supplied by a hypodermic needle of Popper & Sons, ... accuracy of spot positions, optimization and adaptation steps for deposition are reduced to a minimum, since only one chemical compound, neutravidin, is to be deposited Results and Discussion We...

Ngày tải lên: 11/08/2014, 00:22

... presence of external force thermodynamic enthalpy thermodynamic entropy the force acting on the bond Boltzmann's constant dissociation constant equilibrium constant dissociation rate or off rate ... dissociation of the bond in vacuum the natural vibration frequency factor of the association of the bond in vacuum Free energy barrier width or mechanical reaction coordinate life time of molecular bond ... bond x List of Tables List of Tables Table 2-1 Summary of different surface modification and conjugation methods commonly used in AFM tip functionalization 45 Table 3-1 Comparison of knob phenotypes...

Ngày tải lên: 11/09/2015, 14:34

... illustration of the AFM indentation test on cells 20 Figure The effects of deformation force on AFM images of cell cortex 23 Figure The effects of scan direction on AFM images of living MCF-7 cells ... preparation 16 3.4 Atomic force microscopy 17 3.5 AFM imaging 17 3.6 AFM force curves 18 3.7 Young’s modulus calculation of the cells 19   CHAPTER AFM IMAGING STUDY OF MCF-7 CELLS 21 4.1 Introduction ... indentation on living MCF-7 cells 35 vii      Figure 13 Force versus indentation curves for AFM indentation on living MCF-7 cells 37 Figure 14 The effects of emodin on the micromechanical properties of...

Ngày tải lên: 30/09/2015, 14:23

... Introduction One can easily distinguish between two general modes of operation of the atomic force microscope (AFM) depending on absence or presence in the instrumentation of an additional device ... (2001) Subatomic features in atomic force microscopy images Science 291, 2509 Jarvis, M R., Perez, R., and Payne, M C (2001) Can atomic force microscopy achieve atomic resolution in contact mode? ... samples: in fact, quite often, so-called atomic resolution” on crystals is obtained best with standard silicon nitride tips In general, one can choose among one of three types of tip The standard...

Ngày tải lên: 11/04/2014, 02:07

... Introduction Scanning Tunneling Microscopy Atomic Force Microscopy Force Spectroscopy Photonic Force Microscopy References 13 21 30 The Atomic Force Microscope in the Study of Membrane Fusion and ... Introduction Active Q Control Application of Active Q-Control AFM Transverse Dynamic Force Techniques Conclusions References 337 340 344 351 354 354 ix Contents 17 Scanning Force Microscopy Studies on ... Cell Adhesion Measured by Force Spectroscopy on Living Cells Martin Benoit I II III IV V Introduction Instrumentation Preparations of the Force Sensor for Measurements with Living Cells Cell...

Ngày tải lên: 27/06/2014, 08:20

... Introduction One can easily distinguish between two general modes of operation of the atomic force microscope (AFM) depending on absence or presence in the instrumentation of an additional device ... (2001) Subatomic features in atomic force microscopy images Science 291, 2509 Jarvis, M R., Perez, R., and Payne, M C (2001) Can atomic force microscopy achieve atomic resolution in contact mode? ... samples: in fact, quite often, so-called atomic resolution” on crystals is obtained best with standard silicon nitride tips In general, one can choose among one of three types of tip The standard...

Ngày tải lên: 27/06/2014, 08:20

... Introduction Scanning Tunneling Microscopy Atomic Force Microscopy Force Spectroscopy Photonic Force Microscopy References 13 21 30 The Atomic Force Microscope in the Study of Membrane Fusion and ... Introduction Active Q Control Application of Active Q-Control AFM Transverse Dynamic Force Techniques Conclusions References 337 340 344 351 354 354 ix Contents 17 Scanning Force Microscopy Studies on ... Cell Adhesion Measured by Force Spectroscopy on Living Cells Martin Benoit I II III IV V Introduction Instrumentation Preparations of the Force Sensor for Measurements with Living Cells Cell...

Ngày tải lên: 27/06/2014, 17:20