animals cell lines and antibodies
Báo cáo sinh học: "Retroviral vectors encoding ADA regulatory locus control region provide enhanced T-cell-specific transgene expression" potx
Ngày tải lên :
14/08/2014, 19:22
... (Molt4, CEM, CEM-SS, and VB), the murine EL4 Tcell line, and non-T -cell lines (HeLa, 293T, 3T3, and XC) were used to assess the cell- type specific gene expression for the vector SAG and the best performing ... elemental changes made to SAG, SiLAG, and SHGAL in both non-T -cell and T -cell lines (Figure 4C) However, an enhancement of gene expression was observed only in T -cell lines (Figure 4B) Thus, our studies ... various T -cell lines and in the non-T -cell line, 293A, is shown in Figure 5B The SHGI7AL vector yielded enhanced gene expression in all the T -cell lines tested, namely Jurkat, Molt-4 and CEM,...
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Báo cáo y học: ""Shock and kill" effects of class I-selective histone deacetylase inhibitors in combination with the glutathione synthesis inhibitor buthionine sulfoximine in cell line models for HIV-1 quiescence" doc
Ngày tải lên :
12/08/2014, 23:21
... replication in U1 and ACH-2 cells, and toxicity in uninfected Jurkat T-cells Panel A: Cells were incubated with the test compounds (1 μM), and p24 production was measured by ELISA in cell culture ... institutional library of HDACIs [see Additional file 1] in cell lines in which HIV-1 is inducible (i.e T-lymphoid ACH-2 cells and monocytic U1 cells) The potency of these molecules to activate HIV-1 ... HDACIs and corresponding toxicity in U1 and ACH-2 cells Figure (see previous page) Dose-dependent activation of HIV-1 replication by class I-selective HDACIs and corresponding toxicity in U1 and...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
Ngày tải lên :
19/02/2014, 05:20
... of SA on HO-1 and HO-2 expression levels in human cell lines HeLa cells (A and B) and HepG2 cells (C and D) were left untreated or treated with SA (5 mM) for the indicated time and harvested ... myeloid cells, H146 small cell lung cancer cells, and HMV-II melanoma cells H146 small cell lung cancer cells were obtained from ATCC (HTB-173) and cultured in RPMI-1640 medium HMV-II melanoma cells ... each siRNA in HeLa and HepG2 cells HeLa cells (A and B) and HepG2 cells (C and D) were treated for 24 h with siHO-1, siHO-2 or siGAPDH as described in Experimental procedures Cells treated with...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
Ngày tải lên :
23/03/2014, 09:21
... propagated in 1% of initially transfected cells [4] EBV plasmid replication has been demonstrated for a large variety of human cell lines; primate and canine cell lines may also be used [5] It has been ... plasmids and clonal isolation, which makes setting-up an inducible cell line a protracted (at least weeks if one buys cell lines prepared for the final round, or 12 weeks if one starts from scratch) and ... is identical; the 3¢ interface is CAAGTAAA GCGGCCGCGG Cell culture, transfection, and flow cytometry 293 cells (ATCC CRL-1573), a transformed cell line derived from human embryonic kidney, were...
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Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf
Ngày tải lên :
18/06/2014, 19:20
... metformin, from DM, HS and CN for the care of melanoma cell lines and cell viability studies and from BC-A for the optimization and conduct of cell viability, cell cycle, apoptosis and phosphoflow studies: ... of μM vemurafenib and 10 mM metformin of all melanoma cell lines tested The green cell lines harbor BRAFV600E, red shows NRASQ61 mutant cell lines, and black the wild type cell line M257 Propidium ... combination on cell proliferation and viability a) Example of growth curves of BRAFV600E and NRASQ61 mutant melanoma cell lines BRAFV600E cell lines included M263 (synergistic) and M308 (antagonistic)...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
Ngày tải lên :
16/02/2014, 09:20
... responses [2] Th1 cells produce interferon-c (IFN-c) and lymphotoxin, activating cell- mediated immunity and providing protection against intracellular pathogens and viruses Th2 cells secrete interleukin-4 ... between treatments and control groups are significant: *P < 0.05; **P < 0.01 has been shown that various unstimulated murine and human T-cells, B-cells, myeloid cells, monocytic cells and fibroblasts, ... IL-13 and IL-25 (also known as IL-17E), which are important in the generation of the correct class of antibodies by B-cells, and for the elimination of extracellular pathogens, such as helminths and...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
Ngày tải lên :
17/03/2014, 23:20
... IL-2R subunits and HLA glycoproteins in T leukemia and lymphoma cell lines (A) Representative FRET efficiency (E, %) histograms measured on T lymphoma/leukemia cell lines, on cell- by -cell basis, using ... T cell lines by flow cytometry The IL-2R a and cc chains were found constitutively expressed in several (6–10) thousands of copies in all cell lines, except in MT-1, which is deficient in a and ... that of the a and c chains on all the four T cell types (‡ 105 per cell) , characteristic of leukemic or activated T cells HLA-DR was abundant on all cell lines (‡ · 105 copies per cell) Surface...
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Báo cáo khoa học: The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines pdf
Ngày tải lên :
30/03/2014, 11:20
... VRK2 in two cell lines, WSI, derived from normal skin fibroblasts, and MCF7 cells from a breast carcinoma In both cell lines there was strong staining in both the cytosol and nucleus, and there ... (solid lines) or specific VRK2B message (dotted lines) in the H1299 (blue) and MCF7 (pink) cell lines (D) Localization of endogenous VRK1 and VRK2 proteins in three adenocarcinoma cell lines VRK1 ... all cell 2490 lines, but VRK2B protein was more abundant in some cell lines, such as C4-I, HeLa, MCF7 or the colon carcinoma WiDr, and detected in smaller amounts in the remaining carcinoma cell...
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Easy PHP websites with the zend framework (w jason gilmore) (2011)(t)
Ngày tải lên :
23/06/2014, 13:03
... Configuration Files and Parameters Building Persistent Classes Generating and Updating the Schema Querying and Manipulating Your Data Inserting, Updating, and Deleting ... confusing mishmash of PHP code and SQL execution statements Further, constantly donning and removing the PHP developer and SQL developer hats can quickly become tiresome and error prone The Zend Framework's ... should possess a basic understanding of the PHP language, at least a conceptual understanding of object-oriented programming and preferably PHP's particular implementation, and a basic grasp of Structured...
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Báo cáo y học: " DNA methylation patterns associate with genetic and gene expression variation in HapMap cell lines" ppt
Ngày tải lên :
09/08/2014, 22:23
... human lymphoblastoid cell lines (LCLs) from the HapMap collection These cell lines represent a unique resource as they have been densely genotyped by the HapMap Project [21], and are now being genome-sequenced ... JTB, JKPr, and YG wrote the paper and interpreted the results JKPr and YG designed the study JTB analyzed the data AAP performed bisulfite sequencing and sample preparation JKPi mapped and processed ... (d) Methylation levels inside and outside of annotation categories, including CpG Islands (CGIs) for probes within 100 bp of the TSS, and histone modifications and transcription factor (TF) binding...
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báo cáo khoa học: "Co-existence of acute myeloid leukemia with multilineage dysplasia and Epstein-Barr virus-associated T-cell lymphoproliferative disorder in a patient with rheumatoid arthritis: a case report" docx
Ngày tải lên :
10/08/2014, 22:20
... speckled pattern), and negativity for anti-double strand DNA antibody, rheumatoid factor, anti-RNP antibody, and anti-SS-A antibody Bilateral hand X-rays showed mild swelling and destruction of ... (LNs) from autopsy Hematoxylin and eosin (HE) staining and immunohistochemistry analyses of bone marrow (BM), spleen, and lymph nodes Hematoxylin and eosin (HE) staining and immunohistochemistry analyses ... mg/day) and bucillamine (200 mg/day), but discontinued the bucillamine after months due to skin rash and eye lid edema His regular blood tests revealed worsening anemia and thrombocytopenia, and...
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Báo cáo y học: "Systems biology-defined NF-κB regulons, interacting signal pathways and networks are implicated in the malignant phenotype of head and neck cancer cell lines differing in p53 status" pps
Ngày tải lên :
14/08/2014, 08:20
... exons 4-9, and confirmed with western blotting and immunohistochemistry [14] Mutation of p53 was detected in six cell lines, UM-SCC 5, 11B, 22A, 22B, 38 and 46 Four cell lines, UM-SCC 1, 6, and 11A, ... status: W refers to five UM-SCC cell lines with wild-type-deficient status; M refers to five UM-SCC cell lines with mutant p53 status; and W+M refers to ten UM-SCC cell lines ‡Statistical significance ... targets in HNSCC and other cancers with phenotypic and genetic heterogeneity related to p53 status or other abnormalities Materials and methods Cell lines Ten established HNSCC cell lines, UM-SCC...
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THE REGULATORY ROLE OF MATRIX METALLOPROTEINASES IN T CELL ACTIVATION
Ngày tải lên :
24/08/2014, 11:37
... distinguish between the body's own cells, and those that are foreign In the adaptive immune system B cells and T cells (CD4+ and CD8+) are the major cell types present B cells are involved in the humoral ... deficient CD4+ and CD8+ T cells 98 K Production of IL-2, TNF-α, IFN-γ are reduced in SB3CT treated CD4+ and CD8+ T cells 105 L MMP9 deficiency alters CD4+ and CD8+ T cell phenotypes ... treatment and MMP2 and MMP9 deficiency abrogate CD25 mRNA expression in CD4+ T cells 91 xvi Figure 30 Foxp3 and IL-10 expression is elevated in MMP9 deficient and SB3CT treated CD4+ T cells...
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Role of hodgkin and reed sternberg cell derived lymphotoxin alpha in t cell recruitment into the microenvironment of hodgkin lymphoma lesions
Ngày tải lên :
10/09/2015, 09:29
... divide T cells into T helper cells, cytotoxic T cells and regulatory T cells 1.2.1 T helper (TH) Cells There are four basic types of THelper cells: THelper1, THelper2, THelper17 and Treg cells (Figure ... better understand the cell- cell signaling pathways between the HRS cells and the nonmalignant reactive and stroma cells in lymphatic tissues Findings so far pointed out immune cells in the microenvironment ... Hodgkin and Reed-Sternberg cells (HRS cells) surrounded by massive inflammatory infiltrate CD4+ T helper cells, regulatory T cells and CD8+ cytotoxic T cells form a significant part of this cellular...
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The accessory roles of lipopolysaccharide activated murine b cells in t cell polarization
Ngày tải lên :
14/09/2015, 08:27
... Characteristics and functions of B cells 1.4.1 B cells and subsets 38 38 iv 1.4.2 The Antigen presentation function of B cells 1.4.3 Regulatory B cells (Breg) 43 47 1.5 Rationales and specific aims ... Materials and Methods 64 2.1 Mice 2.2 Culture medium, antibodies and lipopolysacharride 2.3 Preparation of single cell suspension 2.4 Splenic B cells purification 2.5 Splenic cell cultures 2.6 B cell ... Introduction 16 1.2.1 Th1 cells, Th2 cells and Th17 cells T cells develop in the thymus Matured circulating T cells that have not yet encountered their antigens are known as naїve T cells When they encounter...
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Tài liệu Báo cáo khoa học: Separation of a cholesterol-enriched microdomain involved in T-cell signal transduction doc
Ngày tải lên :
20/02/2014, 03:20
... vesicles are cholesterol-enriched and contain raft-marker proteins and some T -cell signalling molecules Fig BCh binds to lipid rafts in Jurkat cells BCh-bound Jurkat cells (1 · 107) were treated with ... membranes with a high C ⁄ P ratio of 1.0, and characterized as PS-rich membranes containing GM1 and GM3 Because BCh was first bound to the cell surface and then the cells were subjected to fractionation, ... distributed asymmetrically in polarized cells [11] Immunoelectron microscopy of peripheral blood T-cells shows distinct clustering and segregation of Lck and LAT on the inner leaflet of the plasma...
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Báo cáo khoa học: Internalization of cystatin C in human cell lines docx
Ngày tải lên :
16/03/2014, 06:20
... capacity of cells The human cancer cell lines chosen included one epidermoid carcinoma cell line, three mammary tumour cell lines and one human pancreas adenocarcinoma All five cell lines exhibited ... microscope analysis, the tested cell lines showed different patterns of internalization and intracellular distribution of cystatin C In the MCF-7 and A-431 cell lines, CLSM demonstrated that internalized ... demonstrated that cystatin bound at the cell surface was cleaved by trypsin and washed away After an increase in some of the cell lines could be detected and after 30 the cell fluorescence had increased...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
Ngày tải lên :
23/03/2014, 07:20
... against some human cell lines, such as human keratinocytes (HaCaT cells), human embryo lung fibroblast cells (HEL cells), human nasopharyngeal carcinoma cells (HEp-2 cells) and PMNs in vitro Results ... 48 Time (h) D 100 80 HEL cells 120 72 HEp-2 cells 120 Cell viability (% of control) 100 C Cell viability (% of control) B HaCaTcells 120 Cell viability (% of control) Cell viability (% of control) ... HaCaT cells were incubated with 100 lM each organic selenium compound Cell viability was measured at intervals of 24 h (B) Cell viability of HEL cells (C) Cell viability of HEp-2 cells (D) Cell...
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báo cáo hóa học:" Preclinical evaluation of dasatinib, a potent Src kinase inhibitor, in melanoma cell lines" pptx
Ngày tải lên :
18/06/2014, 15:20
... on (A) invasion and (B) migration in HT144 and Sk-Mel-28 melanoma cell lines Effect of4 Effect of dasatinib on (A) invasion and (B) migration in HT144 and Sk-Mel-28 melanoma cell lines Error bars ... kinase inhibitor suppresses invasion and induces cell cycle arrest and apoptosis of head and neck squamous cell carcinoma and non-small cell lung cancer cells Clin Cancer Res 2005, 11(19 Pt 1):6924-6932 ... both cell cycle arrest and apoptosis In Lox-IMVI, the most sensitive cell line, treatment with dasatinib induced both apoptosis and cell cycle arrest In the other dasatinib responsive cell lines, ...
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