angiogenesis and lymphangiogenesis in colon cancer metastasis

... chemopreventive agents, including resveratrol [26,27], brassinin [28,29], and deguelin [30,31] Resveratrol is present in grapes, red wine and peanuts, brassinin is from Chinese cabbage, and deguelin is from ... apoptosis in SW480 human colon cancer cells To investigate the effects of IxoA on apoptosis, we 5720 used acridine orange ⁄ ethidium bromide staining and DAPI staining and found a marked increase in ... Conjugated linoleic acid inhibits peritoneal metastasis in human gastrointestinal cancer cells Int J Cancer 118, 571–576 Ogata S, Uehara H, Chen A & Itzkowitz SH (1992) Mucin gene expression in colonic...

Ngày tải lên: 23/03/2014, 10:20

... metastasis in colon cancer which was cut into the following three patterns: no hepatic metastasis, metachronous and synchronous liver metastasis We decided to choose single stage IIIB colon cancer ... macrophage infiltration with angiogenesis and prognosis in invasive breast carcinoma Cancer Res 1996, 56(20):4625-4629 19 Pollard JW: Macrophages define the invasive microenvironment in breast cancer ... in colon cancer Clin Cancer Res 2007, 13(5):1472-1479 37 Nagorsen D, Voigt S, Berg E, Stein H, Thiel E, Loddenkemper C: Tumorinfiltrating macrophages and dendritic cells in human colorectal cancer: ...

Ngày tải lên: 18/06/2014, 16:20

... (11.9%), in ascending colon of 14 patients (15.2%), in transverse colon of subjects (8.7%), in hepatic flexure of patients (8.7%), in splenic flexure of patients (7.6%), in descending colon of ... follows: pT1, tumor invading submucosal layer; pT2, tumor invading muscularis propria or subserosa; pT3, tumor penetrating serosa and perivisceral fat; and pT4, tumor invading adjacent organs ... flexure of colon, descending colon and most part of sigma colon) was not different from that of the patients undergone right hemicolectomy (caecum, ascending colon and hepatic flexure of colon) [1]...

Ngày tải lên: 09/08/2014, 07:21

... suppressor by inhibiting tumor cell motility, invasion, metastasis and angiogenesis [12] Maspin expression is aberrantly silenced in many human cancers including breast, prostate, and thyroid cancer ... roles in angiogenesis: Ang-1 and Ang-4 are agonist ligands for Tie2 and induce tyrosin phosphorylation of Tie2, while Ang-2 and Ang-3 are antagonist ligands They bind to Tie2 without inducing tyrosin ... angiogenesis has not been fully investigated in ovarian cancer In the present study, we examined the relationship between the expression of Ets-1 and its targets Ang-2 and maspin in ovarian cancer...

Ngày tải lên: 10/08/2014, 10:21

... (Hematoxylin staining, × 100) biomarker for colon cancer, gastric carcinoma, lung cancer, and hepatocellular carcinoma [5-8] In this study, we detected high levels of MACC1 in ovarian cancer tissues ... motility, invasiveness and metastasis [18] Deregulated HGF/C-met signaling has been observed in many tumors, including ovarian carcinoma, and been proved to contribute to tumor dissemination and metastasis ... poor prognosis and high risk of metastasis in colon cancer, gastric carcinoma, lung cancer, and hepatocellular carcinoma [5-8] However, the mechanism of MACC1 implicates in ovarian cancer is still...

Ngày tải lên: 10/08/2014, 10:21

... transduced into OV90 cells 103 xiv Figure 33 Wild-type TG2 and N-terminal fibronectin binding domain of TG2 induce EMT in OV90 cells Figure 34 104 Wild-type TG2 and N-terminal fibronectin binding domain ... hinge region, which is between the N-terminal ECM binding domain and the C-terminal LAP binding domain This can be achieved by many proteinases in the serine proteinase family, such as plasmin, ... Tyr583 are involved both in GDP and ATP binding to TG2 The residue Arg580, which is important for GTP/GDP binding, is not involved in ATP binding (Han et al 2010) By purifying the insulin-like growth...

Ngày tải lên: 24/08/2014, 10:58

... 10% of all cancer incidence and mortality in 20132 Consistent with trends in developed countries, CRC is the most frequent cancer in Singapore, accounting for 14% to 18% of all cancer incidence ... 2005-20093 Colorectal cancers usually begin as non-cancerous polyps in the inner lining of the colon or rectum4 Over many years, adenomatous polyps in the dysplasic colon may develop into pre-neoplastic ... to initiate metastatic processes in vitro, through promoting metastatic protein expression, as well as modulating oncogenic and mesenchymal transcription STMN1 silencing on the other hand reinstates...

Ngày tải lên: 09/09/2015, 11:25

... carboxyl-terminal tail as illustrated in Figure 1.5C appears to be the docking site for at least two interacting sites including calmodulin (CAM) binding sites and region involved in microfilament-associated ... Changes in levels of phospho-proteins and non-phospho proteins upon 4α-PDD stimulation for 15 mins and 16hrs in 4T07 cell line 80 3.27 Changes in levels of phospho-proteins and non-phospho proteins ... TRPV4 in invasive human breast cancer cell lines and tissues 37 37 42 TRPV4 is a positive regulator of breast cancer metastasis 3.2.1 Function of TRPV4 in breast cancer cell movement, invasion and...

Ngày tải lên: 02/10/2015, 17:15

... β1 integrin and Src kinase have also been implicated in the role that uPAR plays in enhancing colon cancer metastasis Several integrins, osteopontin (OPN), SRC activation, and carcinoembryonic ... cycle of in vivo passaging, there was an increase in the resulting liver metastasis nodule, signifying increasing metastatic propensity of the cell lines Liver metastasis were obtained in out of ... INTRODUCTION 1.1 Incidence, staging & survival rate of colon cancer Colon cancer is the second most common cancer in both gender groups, second only to lung and breast cancers in the male and female...

Ngày tải lên: 07/10/2015, 10:02

... Expression of IDO and Bin1 in colon cancer Expression of IDO and Bin1 in colon cancer IDO is expressed in primary colon cancer cells (A), in adjacent non-cancerous epithelium (B) in lymph node metastases ... epithelium, and immune cells The staining of IDO occurred on both the luminal and basal surfaces of tumor cells and normal epithelial cells In the same section, staining of IDO occurred both in cancer ... moderate staining (score = 2), and intense staining (score = 3) The labeling frequency was scored as (≤ 5%), (5% to 25%), (26% to 50%), (51% to 75%), and (≥ 76%) The product index was obtained by...

Ngày tải lên: 18/06/2014, 15:20

... lesions covering < 50% body surface area (BSA) Intervention indicated Interfering with ADL Interfering with ADL Intense or widespread and interfering with ADL Severe, generalized erythroderma, ... relationship and in providing these groups of patients with accurate and truthful information In respect to comparative data of subjects with skin clinical problems, our sample of colon cancer patients ... point instead of prospectively evaluating toxicities' impact on aspects of life over time Finally, considering the clinical conditions of patients included in this study (mainly metastatic and...

Ngày tải lên: 20/06/2014, 16:20

... activity levels in cells treated with cisplatin and oxaliplatin in combination with doxycycline (p = 0.001 and p = 0.039 respectively) These findings corresponded with the findings obtained in caspase ... apoptosis in colorectal cancer cell lines [13,14] In this study we investigated whether doxycycline works in synergy with cisplatin and oxaliplatin or not in HT 29 colorectal cancer cell line Methods ... with cisplatin and oxaliplatin alone However its level decreased in time dependent manner in HT 29 cells treated with combinations of cisplatin or oxaliplatin and doxycycline (p = 0.066 and p = 0.001...

Ngày tải lên: 09/08/2014, 04:20

... procured locally Cell line and culture conditions The human lung cancer cell line A549 was obtained from the China Center for Type Culture Collection (Wuhan, China) and maintained in RPMI-1640 supplemented ... anticancer effect of Osthole has been reported in a few papers Both in vitro and in vivo studies showed that Osthole possessed an anticancer effect by inhibiting human cancer cells growth and inducing ... Cdc2/Cyclin B1, Bcl-2 protein and up-regulated Bax in our study In summary, these results indicated that Osthole induced G2/M arrest and apoptosis possibly by down-regulating Akt signaling in human...

Ngày tải lên: 10/08/2014, 10:21

... washed in PBS and fixed with 4% paraformaldehyde for 15 at 4°C in PBS Cells were washed in incubation buffer (0.2% SAB, 0.1% saponin in PBS containing 0.2% of sodium azide) then incubated in 200 ... effective in synchronizing DHDK12 cells in S phase than ara-C or aphidicolin Consequently, the efficacy of MTX in synchronizing cells in S phase was then tested in the HT29 cell line In HT29 cell line, ... determinate by staining cells with annexin V-FITC and propidium-iodide (PI) labeling, because annexin V can identify the externalization of phosphatidylserine during the apoptotic progression and...

Ngày tải lên: 10/08/2014, 10:21

... cervical and ovarian cancer EZN-2208 undergoing evaluation in phase trials for treatment of metastatic breast and colorectal cancer (www.clinicaltrials.gov) Doxorubicin and Anthracyclines Inhibits binding ... that can influence cell survival and growth through changes in several signaling pathways that are involved in protein synthesis, autophagy, apoptosis and metabolism [29] Intriguingly, mTOR and HIF1 ... population, both in stabilized cancer cell lines and in primary cells [88], where it promotes colony formation and tumorigenesis Moreover, miR-495 is involved in main­ enance of the cancer stem cell...

Ngày tải lên: 11/08/2014, 12:21

... N-terminal kinase activation in Docosahexanoic acid-induced apoptosis in colon cancer cell In preparation • Manav, HM Shen and CN Ong Docosahexanoic acid inhibits TNF-α induced COX-2 expression in ... macrophages and colonocytes, and plays a critical role in regulating adipocyte differntiation (Willson et al., 2001) The three PPAR isoforms (α,δ,γ) differ in their C-terminal ligand binding domains, and ... appears to bind and respond to a specific subset of agents including hypolipidemic drugs, long chain fatty acids, prostaglandins, and antidiabetic thiazolidinediones Upon ligand binding, the receptor...

Ngày tải lên: 26/09/2015, 09:57

... receptor protein and intracellular cofactor FK506-binding protein 12 (FKBP12) and directly binds to and inhibits TOR proteins through an allosteric site N-terminal to its kinase domain Called the ... kinase domain inhibitors in preclinical and clinical studies (Menon and Manning 2008) These will be discussed in detail in the context of rapamycin‟s status in preclinical and clinical trials (section ... gained by mTOR activation in cancer cells and the increasing knowledge on the implications of mTOR in promoting and maintaining malignant transformation have placed mTOR as a critical anticancer...

Ngày tải lên: 05/10/2015, 13:53

... PROTEIN BINDING STUDY 5.1.1 Introduction The binding of drugs to plasma and tissue proteins is a major determinant of drug distribution throughout the body Protein binding displacement interactions ... and COL-3 bind mainly to albumin So protein-binding interaction, where competition for the protein-biding site involves, could be a good reason to explain this phenomenon Some cases of protein ... examine if the in vitro protein binding of COL-3 would be affected by tamoxifen 5.1.2 Materials And Methods The serum protein binding of COL-3 in the absence and presence of tamoxifen was determined...

Ngày tải lên: 09/10/2015, 11:24

... Tumor Angiogenesis in Gastric Cancer Cancer Microenviron 2009 in press 14 Waugh DJ, Wilson C: The interleukin-8 pathway in cancer Clin Cancer Res 2008, 14(21):6735-41 15 Nishimoto N: Interleukin-6 ... assay and GEM assay experiments, assisted in data analysis and interpretation and in writing and revising the manuscript SP and JG carried out the Western blot experiments CH and SA assisted in ... been involved in the expression of VEGF in endothelial cells [19], induction of angiogenesis [20], tumor angiogenesis in gastric cancer [13], and acquisition of chemotherapeutic resistance in androgenindependent...

Ngày tải lên: 11/08/2014, 00:22

... mRNA and protein levels in treated HT29 and HT29-R cells (Fig 5A,B) Then, HT29-R cells were incubated with increasing concentrations of the TOP1 inhibitor, camptothecin, in the presence and in ... down-regulating vimentin in the absence and in the presence of MTX Targeting of vimentin was carried out by lipofecting aODN-VIM into parental HT29 cells causing a decrease of 60% in the expression ... would involve a multitargeted antifolate Indeed, a novel inhibitor, pemetrexed, now in phase II trials, inhibits at least four enzymes involved in folate metabolism and purine and pyrimidine synthesis:...

Ngày tải lên: 07/03/2014, 16:20