and the circadian clock gene toc1 after application of dex

... the number of NCED and ZEP genes in the maize genome, to map the genes on the LHRF mapping panel and to determine their expression patterns in the kernel of both maize parental inbred lines The ... these in silico studies, six members of the NCED gene family and two members of the ZEP gene family were identified and mapped by PCR amplification and sequencing Third, expression profiles of ... moisture Furthermore, the expression of many LEA genes and more generally members of the Responsive to ABA (Rab) gene family, is induced by exogenous ABA [18] The ABA biosynthetic and catabolic...

Ngày tải lên: 12/08/2014, 03:21

... mRNA isoforms The rectangles represent the exons and the horizontal bold lines the introns The black parts of the rectangles show the protein-coding region, the white parts represent the non-coding ... (SpuUn_WGA56223_2:75333–76952) and Nematostella vectensis (NW_001834409:c1450413–1449631) The numbers above the boxes indicate the size of the exons, and those below the lines indicate the size of the introns (B) ... structure and expression analysis of the Cc RNase ment with the terminal sequences of the longer transcript was amplified by PCR and the RNase j gene structure was determined The C capitata RNase j gene...

Ngày tải lên: 23/03/2014, 06:20

... levels in the presence of core was the result of an overloading of the cellular protein-synthesis machinery and of a shortage of its components Finally, we examined the possible effect of core+1 ... Expression of the HCV-1 core+1 protein potential functions for the core+1 protein with regard to the regulation of microtubule dynamics and mitosis The biological role of the core+1 protein(s) and their ... with the modulation of multiple genes involved in lipid metabolism [69] The location of the core+1 ORF within the viral genome, the findings that the core+1 ORF can be expressed independently of the...

Ngày tải lên: 30/03/2014, 03:20

... result of their concern of having lost something So they wander about and aimlessly search through drawers and closets Some patients rise anxiety for fear of losing track of their caregivers So they ... twisted and dilated and probably detach from the proximal stem Meanwhile, NFTs appear in the cell body In the final stage of the deterioration, after years of development in the cells the NFTs ... identification of the canonical aging changes and further research on why and how they happen may be a promising way to understanding the phenomena of aging And we may have another way to achieve...

Ngày tải lên: 05/10/2015, 22:32

... result of their concern of having lost something So they wander about and aimlessly search through drawers and closets Some patients rise anxiety for fear of losing track of their caregivers So they ... twisted and dilated and probably detach from the proximal stem Meanwhile, NFTs appear in the cell body In the final stage of the deterioration, after years of development in the cells the NFTs ... identification of the canonical aging changes and further research on why and how they happen may be a promising way to understanding the phenomena of aging And we may have another way to achieve...

Ngày tải lên: 06/10/2015, 20:35

... within the maize SUN genes showed that the gene structures are conserved within each class The CCSD genes had two or three exons, and the SUN domain was split between the exons On the other hand, the ... SUN-domain protein genes is provided in Table and the properties and motifs of the CCSD and PM3 subfamilies of these proteins are summarized in Table Conservation of Two Classes of SUN-domain Proteins ... interrupts the SUN domain (between K364 and V365 in the ORF of ZmSUN1 and between K338 and D339 in the ORF of ZmSUN2; Figure 2A) This ancestral intron position may be a hallmark of this class of SUN genes,...

Ngày tải lên: 11/08/2014, 11:21

... on the geometric mean of the expression values of the set of the control genes tested The lower the M value, the more stably expressed the gene is Also, the program enables the exclusion of the ... value of all the other genes and also a change in the M values of the unstable genes, BbrizGDP and BbrizUBI (Figure 3b) To check if the Figure Box-whisker showing the Ct variation of each candidate ... were again the more stable and the least stable genes respectively, while there was a slight difference in the order of stability of the other genes The second and third best reference genes were...

Ngày tải lên: 12/08/2014, 03:20

... interspersed by a stimulus which deviated from the standard in the intensity of the first component and the glide direction of the second constituent The number of distinct MMN components elicited by ... et al The relationship of age, gender, handedness, and sidedness to the size of the corpus callosum Academic Radiology 1994; 1:243-248 69 Driesen NR, Raz N The influence of sex, age, and handedness ... P300 and the N2b Data indicate that the P300 is involved not only in processes of working memory, but may interact with the N2b in the control of motor response to external cues Through the use of...

Ngày tải lên: 02/11/2012, 11:08

... II The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The ... Lys173 interacts with both O6 and N7 (Fig 1C) The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups of the ribose are involved in ... Asp142, 2¢ and 3¢OH of the ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate of the GMP is hydrogen bonded to main chain and side chain atoms of amino...

Ngày tải lên: 15/02/2014, 01:20

... by the cleavage of the bond between 1¢ and 2¢ carbons of the substrate The epimerization product is generated by the re-formation of the C–C bond after the reorientation of GA, which exposes the ... addition of the enzyme, and the middle three spectra were obtained 18, 35, and 70 after the addition of the enzyme The top spectrum is that of DHMP for comparison Only the NMR signals of the 2¢ and 3¢ ... to the sizes of the Fig Stopped-flow analysis of the binding of HPO to SaDHNA The concentration of SaDHNA was lM, and the concentrations of HPO were 10, 20, 30, and 40 lM for traces 1, 2, 3, and...

Ngày tải lên: 19/02/2014, 00:20

... illustrates the overall saddle shape of the dimeric structure and the key heparin-binding residues in the N-terminal domain held coordinately by the two subunits on the top of the hollow (Fig 1A) The ... the C-terminal domain of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional importance of LPL’s C-terminal domain ... not the lipase activity and that the normal affinity of these mutants for heparin (Fig 3, Table 1) implies conservation of the overall structure of the WW and WWW mutants – indicate that these...

Ngày tải lên: 21/02/2014, 15:20

... the sunshine of the evening, the noise of the streets, the looks of the crowd, the great minster rising half-finished in the midst of the town by the Rhine, the cries and noise and chipping of ... Roland and the picture of the dying Renaud, I would fain bring before you before speaking of the Euphorion, by Vernon Lee 15 other Roland and the other Renaud, the Orlando of Ariosto and the ... piles of cabbage and cauli-flowers, the rows of tin ware and copper saucepans, the heaps of maccaroni and pastes, of spices and drugs; the garlands of onions and red peppers and piles of apples; the...

Ngày tải lên: 21/02/2014, 21:20

... tting parameters The adequacy of the analyses was determined from the reduced weighted sum of squares of residuals, and visual inspection of the distribution of weighted residuals The general expression ... global and local A C 5398 dynamics of the AEDANS and W94 probes in free and DNA-bound RepA The decay of the total uorescence intensity was recorded, as well as the decays of its vertically and horizontally ... of the acceptor It is independent of FRET and depends only on the acceptor concentration The extinction coefcients of W94 [25] and AEDANS [26] are assumed constant as a function of labeling and...

Ngày tải lên: 07/03/2014, 04:20

... from each other by alternate highlighting in bold and italics The ATTTA motifs in the 5¢- and 3¢-UTRs, the start and stop codons, the signal peptide predicted by SIGNALP 3.0 [23] and the potential ... the northern blot (Fig 7), and is the largest of the known IL-11 molecules Interestingly, the trout IL-11 transcript has four potential poly(A) signals (AATAAA), two of them present just 14 and ... upstream of the poly(A) tail in the cDNA sequence, which should be the poly(A) signal giving rise to the 3.2 kb transcript The other two are present before the region that contains the ATTTA motifs and...

Ngày tải lên: 07/03/2014, 16:20

... rather reflects synchrony to other events of the spermatogenesis For instance, the mechanisms unleashing the pass of the haploid germ cells through the tight junctions from the adluminal to the ... involved in the normal development of heart and other organs in embryonic and fetal stages The third highest signal in the cDNA dot-blot corresponds to fetal heart and the sixth highest to the interventricular ... causing a partial mixing of venal and arterial blood and a decrease of blood flow to the lungs On the other hand, data from the source database [34] suggests a higher expression of STK33 in neuroblastoma...

Ngày tải lên: 16/03/2014, 23:20

... from the poly(A) of GT-cDNA1 and another is located 11 bp upstream from the poly(A) of GT-cDNA2 (data not shown) Of particular interest is the presence of eight AUUUA motifs in the 3¢-UTR of GT-cDNA2 ... GLUT3 than to other class I GLUTs Comparative analysis of the genomic organization of gcGLUT with different human GLUT genes showed that exons 4–9 of the gcGLUT gene, that encode for the region spanning ... structural homology with six of the respective exons in the hGLUT1 and hGLUT3 Ó FEBS 2003 3016 Z Zhang et al (Eur J Biochem 270) genes, and four of the respective exons in hGLUT2 and hGLUT4 (homologous...

Ngày tải lên: 17/03/2014, 03:20

... at the two spatial tips of the molecule, at the N-terminus with Arg1 and in the turn in the presence of Arg7 and Arg9 (see Figs and 4) The shorter analogue, BM-2, was the most flexible of all the ... of the peptide Two hydrophilic regions were located at the two spatial tips of the molecule, at both termini with Arg1 and Arg17, and in the turn in the presence of Arg8 and Arg10 (see Figs and ... on the membrane This may also account for differences in the haemolytic activity of the peptides A better understanding of the mode of action of these peptides is crucial for the development of...

Ngày tải lên: 22/03/2014, 21:20

... comparison of its MS with that of the reference compound The absolute configuration of glycoses was established by capillary GLC of their acetylated (-)-2-butyl glycosides, according to the method of ... stainless steel needle (27 g) was butted against the low dead volume tee and enabled the delivery of the sheath solution to the end of the capillary column The separations were obtained on  90-cm long ... amount of 2-amino-2deoxy-D-galactose was identified in the hydrolysis products of both carboxyl-reduced CPS and LPS [19], confirming the presence of GalNAcA in the native CPS and LPS This was further...

Ngày tải lên: 23/03/2014, 13:20

... substitution of basic histidine for the asparagine resulted in inactive enzyme Taken together, the analysis of the decarboxylation activity and characterization of the mutants of the putative ... resulting in the formation of the product The FAE1 KCS mechanism has not been characterized and is known to use malonyl-CoA instead of malonyl-ACP Nonetheless, the mechanism of the fatty acid ... identified the active-site cysteine of the jojoba elongase KCS The corresponding cysteine of FAE1 KCS is Cys223 Recently, we confirmed this hypothesis by site-directed mutagenesis of FAE1 KCS [25] Furthermore,...

Ngày tải lên: 24/03/2014, 03:21

... k4.2 The former harbored the complete sequence of the cardosin B gene, whereas the latter enclosed a partial sequence of the cyprosin gene Like other plant AP genes, cardosin genes have their ... plant genes [23] Comparison of cardosin A, B and D genomic clones with the respective cDNAs (Fig 2) revealed the presence of an intron in the 5¢-UTR of the genes The nucleotide sequences of the ... region of the cardosin B gene (from ) 147 bp to + 238 bp) .The 529 bp of the promoter region of the cardosin A gene that is relevant for gene expression in Arabidopsis and the corresponding region of...

Ngày tải lên: 30/03/2014, 09:20