and th2 lymphocytes and cytokines in balf
Báo cáo khoa học: "Comparative studies on the pathogenicity and tissue distribution of three virulence variants of classical swine fever virus, two field isolates and one vaccine strain, with special regard to immunohistochemical investigations" ppt
Ngày tải lên :
12/08/2014, 18:22
... evaluated the findings and influenced the manuscript FF participated in performing the second and third animal experiments inclusive virus isolation and evaluating the results GMDM participated in the ... staining reaction in tonsils, spleen, lymph nodes, lungs and kidneys but not in myocardium and striated muscles Further immunopositivity was detected in nervous tissues in one single animal in ... 3'diaminobenzidine tetrahydrochloride (DAB, Sigma, St Louis, USA) and 0.034% (v/v) hydrogen peroxide for Finally, the sections were rinsed in tap water, counterstained in Mayer's haematoxylin and...
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Báo cáo khoa học: "Comparison of immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assessment for Her-2 status in breast cancer" ppsx
Ngày tải lên :
09/08/2014, 04:21
... results, including variability in tissue fixation and processing, variable sensitivity and specificity of commercially available antibodies, and differences in scoring criteria with considerable interobserver ... variability in interpretation of results [23] Our study is trying to answer a simpler question about the concordance between IHC and FISH in our local setting in Guilin city of China, using some ... methods used in clinical diagnosis, and certain kits or antibodies are approved by the FDA (U.S Food and Drug Administration) IHC is easy to perform and relatively cheap, and is predominantly used...
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ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
Ngày tải lên :
05/09/2013, 08:40
... (PCR) using a diverse set of primers and separated by several fingerprinting techniques including denaturing gradient gel electrophoresis (DGGE) (Muyzer and Smalla, 1998), temperature gradient ... of ammonia- and nitrite-oxidizing bacteria and the microprofiles of NH4+, NO2-, and NO3- in an autotrophic nitrifying biofilm that was initially fed with enrichment culture of nitrifying bacteria ... fluorescence in situ hybridization (FISH) and microsensor techniques have a high spatial (at a single-cell resolution) and temporal resolution and great potential, and provide reliable and direct information...
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prins and in situ pcr protocols
Ngày tải lên :
11/04/2014, 10:11
... achieved by several techniques based either on the intrinsic staining properties of the chromosomes in producing bands (the banding pattern being specific for each pair of chromosomes) (1) or ... chromosome and centromeres with biotin/PO-DAB (brown) and digoxigenin/APase-fast red (red), respectively, in a human lymphocyte metaphase spread after double-target PRINS, hematoxylin counterstaining and ... chamber, and incubate for h at 37°C in an incubator 11 Dehydrate the slides as described in step and au-dry before running the next PRINS reaction with another primer and different reporter 12 Finally,...
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Báo cáo hóa học: " A Novel Method to Fabricate Silicon Nanowire p–n Junctions by a Combination of Ion Implantation and in-situ Doping" docx
Ngày tải lên :
22/06/2014, 00:20
... should result in B-doped—intrinsic (p–i) type NWs, since the boron diffusion in silicon is negligible at 525°C [7], i.e., the B atoms incorporated in the lower half will not diffuse into the upper ... are in direct contact with the p–n junction Therefore we think that surface recombination is indeed playing a major role in the current conduction mechanism across the p–n junction resulting in ... Conclusion In conclusion, we have demonstrated a novel method to form p–n junction NW diodes by combining two well- 123 established doping techniques in- situ doping and ion implantation, in succession...
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Báo cáo y học: "yclooxygenases and prostaglandin E2 receptors in growth plate chondrocytes in vitro and in situ – prostaglandin E2 dependent proliferation of growth plate chondrocytes" ppsx
Ngày tải lên :
09/08/2014, 08:22
... analysis and interpretation of data and was involved in drafting the manuscript CJK was involved in data interpretation, drafting the manuscript and revised it critically for the physiological and ... hormones, 1,25 (OH)2D3 and parathyroid hormone, is dependent on an increase in intracellular calcium and activation of protein kinase C [21] On the other hand, an increase in intracellular cAMP concentration ... formation and promotes DNA and matrix synthesis in growth plate chondrocytes [7] In addition to various findings in vitro, the physiological role of prostaglandins was clarified by its stimulating...
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Báo cáo y học: " Caveolin-1 and -2 in airway epithelium: expression and in situ association as detected by FRET-CLSM" pps
Ngày tải lên :
12/08/2014, 16:20
... 20 and washed) and incubated for1 h in 5% normal goat serum containing 5% BSA in 0.005 M phosphate-buffered saline (PBS) Primary antibodies were diluted in 0.005 M phosphate buffer containing ... determine whether cav-1 and cav-2 are in close apposition in airway epithelial cells in situ, thereby indicating an association of both proteins and formation of hetero-oligomers Distinct increase ... agonist binding [21,22] These receptors are involved in the regulation of ciliary function [23,24] In addition, several proteins involved in Ca2+-dependent signaling processes, including the Ca2+-pump...
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First principles calculations and in situ characterizations
Ngày tải lên :
14/09/2015, 14:05
... is cut into slices with mm in diameter, then the slice is thinned into 10 µm around by using grinding and dimpling apparatus, and finally the thin sample is post-thinned using ion milling The ... (GeO2 and Ge3 N4 ), and to study, both experimentally (using XPS and TEM) and theoretically (using first-principles calculations), the electronic, intrinsic defect, and interface properties and ... at the interface may tune band offsets Theoretically, the procedure of obtaining band offset by using first-principle calculations is actually similar to that of using XPS to determine band offsets...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 1
Ngày tải lên :
14/09/2015, 14:10
... consisting of mechanical consolidation, sintering and extrusion Mechanical testing however often exhibits controversial results and the understanding on deformation behavior of fine grained/nc ... milling media (balls and vial), (ii) process control agent (PCA) and (iii) atmosphere during processing Residual porosity may result from poor interparticle bonding during consolidation and sintering ... due to grain-boundary sliding and/ or diffusional mass transfer via grain-boundary diffusion As such, it is essential to understand and investigate the mechanical behaviors in fine grained materials...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 2
Ngày tải lên :
14/09/2015, 14:10
... relatively large final grain size was obtained Enhanced recovery during milling was confirmed by the corresponding low internal strain The internal strain during milling showed inverse grain size dependence ... the reinforcementmatrix interfaces are clean, resulting in strong interfacial bonding; 35 Literature Review in- situ formed reinforcing particles are finer in size and their distribution in the ... vacuum or in a reducing medium such as toluene and performing powder handling in an argon-filled glove box can minimize the atmospheric contamination [117] The reduction in grain size in powder...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 3
Ngày tải lên :
14/09/2015, 14:10
... shown in Figs 3.1(a) and (b) Due to the high concentration of nitrogen in parazine, relatively fast direct nitriding reaction compared to NH3 gas can be maintained during the milling process During ... then incorporated in the interfaces by the pressure welding of the Al powder followed by diffusing into the Al matrices through grain boundaries, dislocations and other defects [25] Milling induced ... milling and a certain amount of released N reacted with Al with the evidence of weak and broad AlN peaks The proportion of AlN in the powder increased with further milling Higher and distinct...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 4
Ngày tải lên :
14/09/2015, 14:10
... strengthening from grain refinement after 10h-MM is more pronounced for pure Mg indicating grain refinement strengthening mechanism dominates in the initial milling of 10h and 20h in the composite ... of milling indicating no apparent influence of second phases and reinforcement on melting with longer milling duration It is interesting to note that milling has no influence on the melting of ... reduction in grain size and introduction of microstrain during milling In Fig 4.1(b), intensities of Mg (100) and (110) peaks for the extruded specimens increase with milling duration, confirming the...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 5
Ngày tải lên :
14/09/2015, 14:11
... strain rates as a function of milling duration are illustrated in Fig 5.4 Generally, gradual increase in ductility was observed with decreasing strain rate for all milling durations and increasing ... ductility is observed with decreasing strain rate and increasing milling duration However, no significant increase in ductility with milling duration at the highest strain Elongation (%) rate is detected ... strain softening [47] Alternatively, reduction in frictional stress of Hall-Petch strengthening as in dislocation unlocking from impurity or alloying atmosphere, or breaking of barriers in grain...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 6
Ngày tải lên :
14/09/2015, 14:11
... constituents, since the Mg grain size is in nanoscale and thus less likely for the reinforcement and in- situ formed particles to exist in the interior of the Mg grain, this effect should be negligible in ... grain boundaries Energy dissipated in both the grain-boundary sliding and interfacial reaction is negligibly small for conventional grain sizes since grain boundaries will act as perfect 143 Investigation ... logarithmic coordinates of and is shown in Fig 6.1 The relationship between stress and creep strain rate is not linear at different temperatures Two outstanding features of the influence of particles...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 7
Ngày tải lên :
14/09/2015, 14:11
... pure Mg samples This finding implies that the grain refinement to a certain length scale diminishes the significance of solid solution strengthening and dispersion hardening 30hMMed composite ... grain sizes and then performing systematic tests under identical conditions of temperature and stress for the purpose of determining the values of the grain size sensitivity, and (c) performing ... Recommendations for future investigation In general, in order to understand the nature and origin of deformation mechanisms at high temperatures, information related to the dependence of strain rate on stress,...
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A systematic approach for preferential crystallization thermodynamics, kinetics, optimal operation and in situ monitoring
Ngày tải lên :
14/09/2015, 18:18
... differential scanning calorimetry (DSC) 69 Table 4.4 Melting points and enthalpies of fusion of (+)-N-methylephedrine and (±) N-methylephedrine; entropy of mixing of (+)- and (-)-Nmethylephedrine in the ... help I am greatly indebted to Chemical and Process Engineering Centre (CPEC, NUS) and Division of Chemical and Biomolecular Engineering, NTU, for providing research facilities Finally, this thesis ... balance modelling (Randolph and Larson, 1988) The newly developed on-line monitoring and controlling techniques provide good tools to facilitate this kind of investigation (Barrett and Glennon,...
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A study on manganese catalyzed epoxidation of styrene using online raman and in situ FTIR monitoring techniques
Ngày tải lên :
26/09/2015, 10:34
... how interesting and fulfilling the actual experience can be Research can be utterly tedious and involves unassuming patience cum perseverance working towards making scientific contribution in ... (a) Chlorinated Mn-porphyrin complex, (b) Mn-porphyrin mediated epoxidation using imidazole and (c) N-alkyl imidazole and benzoic acid Figure 2-7 (a) Iron porphyrin and (b) Iron porphyrin mediated ... the reaction mechanism and carry out kinetic studies using online Raman, in- situ FTIR, 13C NMR and UV-Vis spectroscopy coupled with application of Band Target Entropy Minimization (BTEM) chemometric...
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Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf
Ngày tải lên :
29/03/2014, 21:20
... cooperatively in the cross-linking of proteins, including involucrin, loricrin and small Preferred substrate peptide for TGase proline-rich proteins (SPRs) Such concerted reactions result in formation ... cross-linking structural proteins in keratinocytes [7–9] TGase is expressed in the prostate and is reported to be involved in plug formation in rodents [10] The biochemical characterization and ... +3 (relative to the glutamine residue); (b) lysine or arginine mainly was found at position +2; and (c) in most sequences, an aromatic amino acid and a serine ⁄ threonine residue were frequently...
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in situ detection of dna damage, methods and protocols
Ngày tải lên :
11/04/2014, 09:47
... counterstain Incubate cells in 0.2 µg/mL DAPI, and rinse once in PBS To label fixed samples using both TUNEL and immunofluorescence staining for an antigen of interest, the antibody incubations ... with a suitable bonding agent that maintains them in place and can withstand the multiple wash steps of the staining procedure, but does not interfere chemically with the staining or contribute ... DNA making it ideal for labeling fragments in apoptotic cells The enzyme will also label single stranded DNA molecules containing a 3'-OH and will attach nucleotides to a single-strand nick in DNA...
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Báo cáo hóa học: "Nearly Monodispersion CoSm Alloy Nanoparticles Formed by an In-situ Rapid Cooling and Passivating Microfluidic Process" docx
Ngày tải lên :
21/06/2014, 20:20
... Flow and temperature controller P1 V1 CoCl2 and SmCl3 mixture P2 H1 H2 Inlet V2 Reducing agent and stability Inlet TC1 N2 In N2 In Y-mixer N2 Out Reaction channel N2 Out TC2 N2 Out P3 N2 in V3 ... formation of an ultra-thin oxidation layer caused by including acetone in the quenching solution EDS data for the resulting NPs indicate the elemental oxygen appearing in those NPs (Fig 3d) Analysis ... from New Teacher Funds (2008-00061025) and SRF for ROCS and SEM by the Chinese Education Ministry, and Innovative Research Team of Chinese Education Ministry in University (IRT0512) at Beihang University...
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