and psilocin containing fungi using dna profiling

... two separate EBV standards, thus reducing costs and labour The EBNA-1 and BHRF-1 DNA targets were linked using randomised primers (Table 1) and inserted into the pGEM vector, using the pGEM®-T ... and BHRF-1 DNA targets into plasmid vector pGEM and standard curve construction A novel feature of the assay was the design of a quantification standard incorporating both EBNA-1 and BHRF-1 DNA ... products for EBNA-1 DNA and BHRF-1 DNA were identified on agarose gel by 213 bp and 208 bp bands, respectively Reproducibility studies consisting of triplicates of each standard curve dilution...

Ngày tải lên: 12/08/2014, 01:22

... for his guidance and support I would like to thank everybody at the lab for their advice and friendship It’s the best lab I have been in and everybody has been great They are past and present Guang ... being a good friend and helping me ever so often Xue Li for her expert assistance and comments Sravan for his advice and suggestions I am also indebted to Dr Robert Yang and all his lab members ... and lipidomics 1.4 Phospholipid profiling 1.5 Yeast as a model organism to study lipids 1.6 Objectives of the thesis 10 MATERIALS AND METHODS 2.1 Strains 11 2.2 Plasmids 16 2.3 Growth media and...

Ngày tải lên: 04/10/2015, 16:03

... 1, and 3), BstXI (Lanes 4, 5, and 6), and BglII plus EcoRV (Lanes 7, 8, and 9) M, 1-kb DNA ladder Lanes 1, 4, and 7, 293A cells; Lanes 2, 5, and 8, ADrBMP6 DNA; Lanes 3, 6, and 9, ADhBMP6 DNA ... BMP4 and BMP6 cDNAs were amplified, cloned, sequenced, and identified Recombinant adenoviruses encoding rat BMP4 and BMP6 were constructed and compared with ADhBMP4 and ADhBMP6 in the in vitro and ... thigh and 1.4 × 1011 particles in the other thigh) On Day 35, the rats were euthanized and scanned using CT Axial CT images (1-mm collimation and 1mm table increment) were obtained using the standard...

Ngày tải lên: 31/10/2012, 17:08

... proliferation and promotion of apoptosis in this model Dietary supplement containing Schisandra chinensis has been shown to reduce prostate cancer cell growth and induce apoptosis by inhibiting androgen ... were measured every days using a caliper, and the tumor area was calculated according to the formula: tumor volume (mm3) = d2 x D/2, where d and D were the shortest and the longest diameter respectively ... K for 20 at 37°C and treated with 0.3% H2O2 solution at room temperature Afterwards, they were incubated with TdT buffer containing 40 U/ml TdT and nmol/ml biotinylated-dUTP, and kept in humidified...

Ngày tải lên: 03/11/2012, 09:54

... and angles in degrees 3536 www.chemphyschem.org  2012 Wiley-VCH Verlag GmbH & Co KGaA, Weinheim ChemPhysChem 2012, 13, 3535 3541 Interaction between an Amide and S -Containing Molecules b and ... of this entire problem, the global minithe literature is understandable as it occurs only in tandem mum of the complex between NMA and CH3SH is a product of with other, stronger noncovalent bonds, ... mol1, even though the H and S atoms are separated Each of the three S -containing molecules was paired with by 3.02 The last component with an E(2) above the 0.5 kcal NMA, and the potential energy...

Ngày tải lên: 16/12/2012, 15:21

... rand1 * ( pi (τ ) − xi (τ )) + c * rand * ( p g (τ ) − xi (τ )) (30) where c1 and c are acceleration constants and rand and rand are uniformly distributed random r numbers in [0, 1] The term vi ... where PGi and QGi : generator real and reactive power at i-th bus, respectively; PDi and QDi : load real and reactive power at i-th bus, respectively; Gij and Bij : transfer conductance and susceptance ... dispatching and the stability of EPS, wind energy and intelligent techniques applications in EPS He is author and co-author of about 18 papers in international journals and national and international...

Ngày tải lên: 05/09/2013, 15:28

... The entry and exit section length were 250 mm and 125 mm respectively ASHRAE standard 97-77 [32] recommends the minimum entry and exit length of 5√ (WH) and 2.5√ (WH), i.e 236.6 mm and1 18.3 mm ... section and outlet section respectively The rectangular duct is1375 mm × 287 mm × 197 mm and fabricated with the red marandi wood and 19 mm thick plywood panel The test section is of 1000 mm long and ... respectively Verma and Prasad [19] investigated the effect of transverse wire roughness on heat and flow characteristics of two roughened collectors and one plane surface collector Saini and Verma [20]...

Ngày tải lên: 05/09/2013, 15:28

... activated on DNA (Kumagai et al 2004), how ATR is stimulated by DNA or its regulators on DNA remains to be elucidated 2.3 Processing of DNA Lesions Single-stranded DNA is a common DNA structure ... substrate of ATM and ATR during DNA damage and DNA replication checkpoint responses J Biol Chem 279:53353–53364 You Z, Kong L, Newport J (2002) The role of single-stranded DNA and polymerase alpha ... Long stretches of ssDNA are generated at stalled replication forks ATR-ATRIP, Rad17, and 9-1-1 complexes are recruited to RPA-ssDNA and junctions of double/single-stranded DNA at stalled forks...

Ngày tải lên: 25/10/2013, 21:20

... functions and conditional mass functions using Equations 14.10 and 14.14 Finally, the fuzzy transition probability and limiting fuzzy transition probability can be calculated using Equations 14.42 and ... function defined over {t, f, u} (t represents true, f represents false, and u stands for uncertain) Let mg = {Li: li} and mg′ = {Mi: mi} and form a matrix {( } ) ( M = T Li / M j : li m j , where T Li ... [0.3, 0.7] 14.3 Monitoring and Diagnosing Manufacturing Processes Using Fuzzy Sets 14.3.1 Using Fuzzy Systems to Describe the State of a Manufacturing Process For monitoring and diagnosing manufacturing...

Ngày tải lên: 25/12/2013, 19:15

... interpretation and in the writing HOK and HSK performed the analysis of the data and drafted the manuscript JCY and ECS participated in the design of the study and performed the statistical analysis and ... (Tables and and Figure 1) TGF-a binds to the EGF receptor with a high affinity [21] and is indispensable for the proper development of many tissues and organs, wound healing, bone resorption, and ... aging (Tables and and Figure 1) The CD40/CD40L system belongs to the tumor necrosis factor superfamily and is a key pathway that links inflammation and atherothrombosis [17] CD40 and CD40L are...

Ngày tải lên: 14/02/2014, 06:20

... probability of CE-candidates using MEM and SVM Both MEM and SVM showed outstanding performance; there was no significant difference between the two machine learning methods (SVM and MEM) Hence, ... as a candidate for SE/OE, and each of adjective (or verb) words as a candidate for PR However, this candidate detection has serious problems as follows: 4) There are many actual SEs, OEs, and PRs ... (Task 2) Entities and This section explains how to extract comparative entities and predicates Our strategy is to first detect Comparative Element candidates (CEcandidates), and then choose the...

Ngày tải lên: 20/02/2014, 04:20

... and HJ 25 contained HJ1, 2, and Three way junctions were prepared using HJ5, and and duplex DNA annealed using D1 and D2, ASP1 and ASP2 All oligonucleotides used in the binding studies are shown ... purified using denaturing PAGE and subsequently evaporated to dryness and desalted using a Pharmacia NAP 10 column according to the manufacturer’s instructions Synthetic Holliday junctions, HJ24 and ... comprised four 24-mer and four 50-mer oligonucleotides DNA annealing and purification were essentially as described previously [33] HJ50 contained HJ5, 6, and HJ 25 contained HJ1, 2, and Three way junctions...

Ngày tải lên: 21/02/2014, 01:21

Ngày tải lên: 16/03/2014, 03:20

... service and economic growth and capital inflow is provided Section presents a research design and describe and specify our simultaneous equations In section 5, theoretical expectations and hypothesis ... framework and tested various direct and indirect relationships between external debt, investment and economic growth He estimated models and carried out simulations for Argentina during 1962-1986 using ... credit, and the greater the number of incidences of financial distress and liquidation adversely affecting the GNP directly and indirectly through discouraging the GNP level directly and indirectly...

Ngày tải lên: 17/03/2014, 08:20

... for N, P, K by using wastewater and river water for irrigation (kg ha-1) Two horizontal lines show the nutrition demand for N, P, K of rice and maize H and L indicated the demand supplying of ... 0.29±0.10 pH, SS and COD The water quality determined is summarized in Table The pH ranged between 7.20 and 7.87 The chemical oxygen demand (COD) and suspended solid (SS) in water were high, and much ... analyzed for pH, chemical oxygen demand (COD), N, P, K, Cd, Cu, Pb and Zn pH was measured by using pH meter immediately after sampling, chemical oxygen demand (COD) was used K2Cr2 O7 method Metal...

Ngày tải lên: 22/03/2014, 12:20

... distinctly and giving such other instruction as may be needed In preparing the lesson the pupil should learn and adopt the preferred pronunciation only, using the other forms for reference and general ... dictionaries of the English language published in this country and in England have been selected for reference The scholarship, labor, and care displayed in these works entitle them to our highest ... indicated might with equal propriety be preferred and used by others In cases where there is a marked difference in the English and the American usage, and yet nearly an average balance, the preference...

Ngày tải lên: 22/03/2014, 16:22

... glass columns, and flushed with hexanes prior to use Preparative chromatography was also carded out using a Chromatotron using glass plates coated with silica gel (P F 254 60) of and mm thickness ... processes and a general guide to functional group compatibility see: (a) Molander, G A Organic Reactions; Paquette, L A., Ed.; John Wiley and Sons: New York, 1994; Vol 46, pp 211-367 (b) Brandukova, ... br s, dd, dq, br t and ABq stand for the resonance multiplicity singlet, doublet, triplet, quartet, broad singlet, doublet of doublets, double of quartets, broad triplet, and AB quartet, respectively...

Ngày tải lên: 22/03/2014, 18:20

... where Cp,N2 and Cp,U stand for the molar heat capacities of the native and unfolded states, respectively; that is, the changes of HN2 and HU with temperature Substitution of this and Eqn (10) ... bind and pack the highly charged DNA and thus the high positive charge on the surface of HU’s may play a role not only in their stability but also in promoting a better interaction with DNA and ... several intertwined a-helices, a pair of triple-stranded b-sheets and two Ôdisordered armsÕ, which are quite flexible in the absence of DNA and not very clearly defined in the X-ray picture of...

Ngày tải lên: 23/03/2014, 12:20

... Sciences DNA- modifying enzymes were from BioLabs, and in vitro transcription and translation kits from Promega All other chemicals and enzymes were from Sigma or from Fisher Scientific and were ... identified as DnaK by N-terminal amino-acid sequencing However, preliminary data obtained using DnaK-deficient mutants suggest that DnaK is not essential for the translocation of HiPIP in vivo, and it ... °C The pellet was resuspended in 50 mM Tris/acetate, pH 7.6, containing 250 mM sucrose, mM dithiothreitol, and 50 lgÆmL)1 DNase I, and passed through a French pressure cell operating at 27.6 MPa...

Ngày tải lên: 23/03/2014, 21:20

... in lanes and 7; 0.13 lL in lanes and 8; 0,25 lL in lanes and 9; 0.5 lL in lanes and 10; lL in lanes and 11; lL in lanes and 12) The ERRc DNA complexes are marked by an arrow, the ERRc DNA complexes ... fusion proteins containing the DNA- binding domain of the androgen and glucocorticoid receptor with andro- DNA binding of ERRc (Eur J Biochem 269) 4097 53 54 55 56 57 58 59 60 gen and glucocorticoid ... binds also as a dimer to DNA [35] For ERRa and ERRb, monomeric and dimeric binding has been demonstrated [3] The repeat nature of the binding site, and the fact that ERRc DNA complexes have a mobility...

Ngày tải lên: 31/03/2014, 09:20