... D1 and D2 receptor fusion proteins (D1–CFP and D2–YFP) incomparison with emission spectra of the cell popula-tions that co-express dopamine D1 receptor fusion protein (D1–CFP) and ... D1 receptor fusion proteins (D1–CFP and D1–YFP).cMeasured in cellco-expressing two dopamine D1 receptor fusion proteins (D1MUT–CFP – genetic variants of dopamine D1 receptor and ... D1 receptor. bMeasured in cell co-expressing dopamine D1 and D2fusion proteins (D1–CFP and D2–YFP).cMeasured in cellco-expressing dopamine D1 and D2fusion proteins (D1–CFP and D2R1–YFP...
... I)-X(6)8)-Y-X-X-(L or I) and arefound in the intracellular portions of the TCR complex c,d, e and f chains, as well as in other immunoreceptorsincluding the B-cell receptor [3] and several Fc receptors[4,5]. ... without washing.NMR of His-cTCRfThe protein was resuspended in 500 lLH2Oand50lLD2O, and the pH adjusted to 6 with dilute HCl. Standard1D and 2D NOESY and DQF-COSY experiments wererecorded ... mgg@soton.ac.ukAbbreviations: ITAM, immunoreceptor tyrosine-based activation motif; His-cTCRf, histidine-tagged cytosolic TCRf;PTK ,protein tyrosine kinase; SH2, src homology domain 2; TCR, T-cell receptor. (Received...
... n2Þwhere [h]1 and [h]2correspond to the measured mean ellip-ticity values per residue of proteins 1 and 2, respectively, and n1 and n2correspond to the number of residues ofproteins 1 and 2.When ... con-tent of unordered stretches (50–59%) and a low con-tent in a-helices and b-strands (2–3% and 22–29%,respectively), whereas GFP is rich in b-strands (46%) and exhibits a low content in unordered ... indi-vidual proteins. M, molecular weight markers; TF, total protein frac-tion; SOL, soluble protein fraction; proteins purified by immobilizedmetal affinity chromatography (IMAC).Ordered and disordered...
... datasetof all protein RNA structures. This kind of methodwas proposed by Sipple [20], and has been applied to protein structure prediction, proteinproteinand pro-tein–ligand interactions ... number of groupsfor the prediction of protein structures, protein DNA and protein ligand interactions [18–35], such anapproach has never been applied to protein RNAinteractions.In the present ... energetic of protein RNA complexes.Knowledge-based potential functions have beenemployed in protein structure prediction [18–27], aswell as in the prediction of proteinprotein [25,28–30] and protein ligand...
... LPS/IFN-inducedNO production Protein kinases such as PKA and PKC, and various factorssuch as tumor necrosis factor receptor 6 (TRAF6) and myeloid differentiation protein (MyD88) are reported toparticipate ... mitogen-activated protein kinase kinase;MyD88, myeloid differentiation protein; NF-jB, nuclear factor-kappaB; NO, nitric oxide; PKA, protein kinase A; PKC, protein kinase C;PP2A, protein phosphatase-2A; ... Effects of PKA inhibitors (KT-5720 and H8) and PKC inhibitors(Ro31-8220 and GF109203X) on the enhancement by TS of LPS/IFN-induced NO production in VSMC (A), and Western blot analysis ofPKCa...
... blue and 30 mMdithiothreitol) and then incubatedat 86 °C for 8 min. Typically, cell extracts (5–10 lgof protein) were separated by SDS/PAGE using the standardLaemmli system [30] with 13% and ... Western blot analysis of proteins in samplescollected in (A). 5 lgand1 lg of total protein in samples were separated by SDS/PAGE and immunoblotted with anti-UP12 Ig and anti-GroEL Ig,respectively. ... buffer,proteins were eluted from the resin with buffer C [0.1MTris/HCI (pH 8.0), 1% SDS, 2 mMEDTA and 20 mMdithiothreitol] and analyzed by SDS/PAGE and Westernblotting.Subcloning and deletion...
... ribosomal protein S6 kinase and acetyl-CoAcarboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytesUlrike Krause*, Luc Bertrand and Louis HueHormone and Metabolic ... theprocess of activation of ACC and p70S6K by amino acids isinhibited by okadaic acid and calyculin A, two inhibitors oftype 1 and 2A protein phosphatase, and by incubation ofliver cells ... activate p70 riboso-mal protein S6 kinase (p70S6K) and acetyl-CoA car-boxylase (ACC) involved in proteinand fatty acidssynthesis, respectively. In contrast, the AMP-activated protein kinase (AMPK),...
... assay, and that the relationship between protein concentration and activity was linear (Fig. S1). The protein concentrationswere 1 lm for beryllium fluoride (BeF3) and BeF42)) inhibi-tion and ... in b-cells and neurons, SUR2Ain cardiac and skeletal muscle, and SUR2B in smoothmuscle and some neurons [1]. SUR2A and SUR2B areencoded by splice variants of a single gene, ABCC9, and differ ... state and thusincreased channel activation.Experimental procedures Protein expression and purificationA FLAG tag was inserted into rat SUR2 between Ala1026 and Asp1027. Full-length SUR2A and...
... transthyretin+TLPsequences, three motifs were identified,A’–C’. Motif A’ is equivalent to motif A fromthe TLP motif set. Motif B’ is similar butextended in the N-terminal and C-terminalregions to motif B. Motif C’ ... dublin TLP and zebrafish TLP are found in the flex-ible portions of strands B and C that protrude towardsthe solvent and in the conformation of the long loopconnecting strands D and E [17]. ... C-terminalregions to motif B. Motif C’ is shorter than motif C and its location is shifted towardsthe N-terminus. Motif D is specific to theTLP set of proteins. (C) Motifs A’–C’ weresuperimposed on the tertiary...
... (1980) Exita-tion profiles, absorption and resonance Raman spectraof the carotenoprotein ovorubin, and a resonanceRaman study of some other astaxanthin proteins. JAmChem Soc 102, 6693–6698.19 ... 40,175–215.30 Randall D, Burggren W & French K (1997) Energyacquisition: feeding, digestion and metabolism. In Eck-ert Animal Physiology. Mechanisms and Adaptations(Randall D, Burggren ... quantities of protein uti-lizing the principle of protein- dye binding. Anal Bio-chem 72, 248–274.34 Davis B (1964) Disc electrophoresis. II. Method and application to human serum proteins. Ann...
... cross-linking and mass spectro-metry for mapping three-dimensional structures ofproteins andprotein complexes. J Mass Spectrom 38,1225–1237.7 Wong SS (1991) Chemistry of Protein Conjugation and Cross-Linking. ... thestructural analysis of proteins andprotein complexes and in large scale analysis of the function of genes(proteomics) [1].For mass spectrometric analysis, the proteins and proteomes under study ... Koster1 and Luitzen de Jong11 Biomolecular Mass Spectrometry group, Swammerdam Institute for Life Sciences, University of Amsterdam, the Netherlands2 Laboratory of Protein Biochemistry and Protein...
... estrogen and androgen,because use of antagonists to androgen and estrogenreceptors (flutamide and ICI 182,780, respectively), and letrozol, an inhibitor of the aromatase responsiblefor converting androgen ... and then toestradiol by the P450 aromatase. It has been shownthat approximately 35% of cervical carcinomas expressaromatase [37] and that DHEA binds to the androgen receptor and estrogen receptors ... R(2005) Dehydroepiandrosterone inhibits the prolifera-tion of HUVEC by enhancing the expression of p53 and p21, restricting the phosphorylation of RB, and isandrogen- and estrogen -receptor independent....
... Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia colitransthyretin-related protein Erik Lundberg1, Anders Olofsson2, Gunilla T. Westermark3 and ... tetrameric and monomericstructures.Thermostability andprotein structuresWe have analyzed the structures of hTTR (Protein Data Bank code: 1F41 [12]), sbTTR (Protein DataBank code: 1SN2, [16]) and ... C, and D, of thetetrameric structures were: hTTR, A10–A122, B10–B122, A¢11–A¢122, and B¢10–B¢122; sbTTR, A10–A122, B10–B122, C11–C122, and D10–D122; and ecTRP, A4–A114, B4–B114, C5–C114, and D4–D114.cThe...
... a-helices and 12%b-turns (Fig. 1). Incubation of the protein with dif-ferent ligands such as palmitate, caprylic and arachi-donic acids and retinol in concentrations sufficient tosaturate the protein ... ethanol and embedded in paraffin. ForR. Jordanova et al. Conformation, ligand binding and distribution of nematode protein Ag-NPA-1FEBS Journal 272 (2005) 180–189 ª 2004 FEBS 187Conformational and ... nonpolar and com-pletely isolated from the solvent.Here we analyze the conformational and functionalproperties of Ag-NPA-1 as well as the tissue and cellulardistribution of the protein. The...
... extracts a single band was recog-nized at 43 kDa indicating the presence of the full-length protein, in mitochondria three bands at about42, 43 and 44 kDa were detected, while no bands werevisible ... couldassume that in mitochondria the unprocessed protein, the mature proteinand a post-translationally modified protein might be present. N-terminal sequencing and analysis of peptides by MS could help ... d-CDes protein was fusedwith the C terminus of the green fluorescent protein (GFP) in the pGFP-C vector the fusion protein remained in the cytoplasm (Fig. 4C).Expression studies on the RNA and protein...