and atrmr1 receptor homology regiontransmembrane domainringh2 motif protein

... D 1 and D 2 receptor fusion proteins (D 1 –CFP and D 2 –YFP) in comparison with emission spectra of the cell popula- tions that co-express dopamine D 1 receptor fusion protein (D 1 –CFP) and ... D 1 receptor fusion proteins (D 1 –CFP and D 1 –YFP). c Measured in cell co-expressing two dopamine D 1 receptor fusion proteins (D 1 MUT– CFP – genetic variants of dopamine D 1 receptor and ... D 1 receptor. b Measured in cell co-expressing dopamine D 1 and D 2 fusion proteins (D 1 –CFP and D 2 –YFP). c Measured in cell co-expressing dopamine D 1 and D 2 fusion proteins (D 1 –CFP and D 2 R1–YFP...

Ngày tải lên: 07/03/2014, 03:20

... I)-X (6)8) -Y-X-X-(L or I) and are found in the intracellular portions of the TCR complex c, d, e and f chains, as well as in other immunoreceptors including the B-cell receptor [3] and several Fc receptors [4,5]. ... without washing. NMR of His-cTCRf The protein was resuspended in 500 lLH 2 Oand50lL D 2 O, and the pH adjusted to 6 with dilute HCl. Standard 1D and 2D NOESY and DQF-COSY experiments were recorded ... mgg@soton.ac.uk Abbreviations: ITAM, immunoreceptor tyrosine-based activation motif; His-cTCRf, histidine-tagged cytosolic TCRf;PTK ,protein tyrosine kinase; SH2, src homology domain 2; TCR, T-cell receptor. (Received...

Ngày tải lên: 08/03/2014, 02:20

Ngày tải lên: 23/03/2014, 07:20

... n 2 Þ where [h] 1 and [h] 2 correspond to the measured mean ellip- ticity values per residue of proteins 1 and 2, respectively, and n 1 and n 2 correspond to the number of residues of proteins 1 and 2. When ... con- tent of unordered stretches (50–59%) and a low con- tent in a-helices and b-strands (2–3% and 22–29%, respectively), whereas GFP is rich in b-strands (46%) and exhibits a low content in unordered ... indi- vidual proteins. M, molecular weight markers; TF, total protein frac- tion; SOL, soluble protein fraction; proteins purified by immobilized metal affinity chromatography (IMAC). Ordered and disordered...

Ngày tải lên: 18/02/2014, 04:20

... dataset of all protein RNA structures. This kind of method was proposed by Sipple [20], and has been applied to protein structure prediction, protein protein and pro- tein–ligand interactions ... number of groups for the prediction of protein structures, protein DNA and protein ligand interactions [18–35], such an approach has never been applied to protein RNA interactions. In the present ... energetic of protein RNA complexes. Knowledge-based potential functions have been employed in protein structure prediction [18–27], as well as in the prediction of protein protein [25,28–30] and protein ligand...

Ngày tải lên: 18/02/2014, 16:20

... LPS/IFN-induced NO production Protein kinases such as PKA and PKC, and various factors such as tumor necrosis factor receptor 6 (TRAF6) and myeloid differentiation protein (MyD88) are reported to participate ... mitogen-activated protein kinase kinase; MyD88, myeloid differentiation protein; NF-jB, nuclear factor-kappa B; NO, nitric oxide; PKA, protein kinase A; PKC, protein kinase C; PP2A, protein phosphatase-2A; ... Effects of PKA inhibitors (KT-5720 and H8) and PKC inhibitors (Ro31-8220 and GF109203X) on the enhancement by TS of LPS/IFN- induced NO production in VSMC (A), and Western blot analysis of PKCa...

Ngày tải lên: 22/02/2014, 04:20

... blue and 30 m M dithiothreitol) and then incubated at 86 °C for 8 min. Typically, cell extracts (5–10 lgof protein) were separated by SDS/PAGE using the standard Laemmli system [30] with 13% and ... Western blot analysis of proteins in samples collected in (A). 5 lgand1 lg of total protein in samples were separated by SDS/PAGE and immunoblotted with anti-UP12 Ig and anti-GroEL Ig, respectively. ... buffer, proteins were eluted from the resin with buffer C [0.1 M Tris/ HCI (pH 8.0), 1% SDS, 2 m M EDTA and 20 m M dithiothreitol] and analyzed by SDS/PAGE and Western blotting. Subcloning and deletion...

Ngày tải lên: 22/02/2014, 07:20

... ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes Ulrike Krause*, Luc Bertrand and Louis Hue Hormone and Metabolic ... the process of activation of ACC and p70S6K by amino acids is inhibited by okadaic acid and calyculin A, two inhibitors of type 1 and 2A protein phosphatase, and by incubation of liver cells ... activate p70 riboso- mal protein S6 kinase (p70S6K) and acetyl-CoA car- boxylase (ACC) involved in protein and fatty acids synthesis, respectively. In contrast, the AMP-activated protein kinase (AMPK),...

Ngày tải lên: 22/02/2014, 07:20

... assay, and that the relationship between protein concentration and activity was linear (Fig. S1). The protein concentrations were 1 lm for beryllium fluoride (BeF 3 ) and BeF 4 2) ) inhibi- tion and ... in b-cells and neurons, SUR2A in cardiac and skeletal muscle, and SUR2B in smooth muscle and some neurons [1]. SUR2A and SUR2B are encoded by splice variants of a single gene, ABCC9, and differ ... state and thus increased channel activation. Experimental procedures Protein expression and purification A FLAG tag was inserted into rat SUR2 between Ala1026 and Asp1027. Full-length SUR2A and...

Ngày tải lên: 06/03/2014, 11:20

... transthyretin+TLP sequences, three motifs were identified, A’–C’. Motif A’ is equivalent to motif A from the TLP motif set. Motif B’ is similar but extended in the N-terminal and C-terminal regions to motif B. Motif C’ ... dublin TLP and zebrafish TLP are found in the flex- ible portions of strands B and C that protrude towards the solvent and in the conformation of the long loop connecting strands D and E [17]. ... C-terminal regions to motif B. Motif C’ is shorter than motif C and its location is shifted towards the N-terminus. Motif D is specific to the TLP set of proteins. (C) Motifs A’–C’ were superimposed on the tertiary...

Ngày tải lên: 07/03/2014, 00:20

... (1980) Exita- tion profiles, absorption and resonance Raman spectra of the carotenoprotein ovorubin, and a resonance Raman study of some other astaxanthin proteins. JAm Chem Soc 102, 6693–6698. 19 ... 40, 175–215. 30 Randall D, Burggren W & French K (1997) Energy acquisition: feeding, digestion and metabolism. In Eck- ert Animal Physiology. Mechanisms and Adaptations (Randall D, Burggren ... quantities of protein uti- lizing the principle of protein- dye binding. Anal Bio- chem 72, 248–274. 34 Davis B (1964) Disc electrophoresis. II. Method and application to human serum proteins. Ann...

Ngày tải lên: 07/03/2014, 06:20

... cross-linking and mass spectro- metry for mapping three-dimensional structures of proteins and protein complexes. J Mass Spectrom 38, 1225–1237. 7 Wong SS (1991) Chemistry of Protein Conjugation and Cross-Linking. ... the structural analysis of proteins and protein complexes and in large scale analysis of the function of genes (proteomics) [1]. For mass spectrometric analysis, the proteins and proteomes under study ... Koster 1 and Luitzen de Jong 1 1 Biomolecular Mass Spectrometry group, Swammerdam Institute for Life Sciences, University of Amsterdam, the Netherlands 2 Laboratory of Protein Biochemistry and Protein...

Ngày tải lên: 07/03/2014, 12:20

... estrogen and androgen, because use of antagonists to androgen and estrogen receptors (flutamide and ICI 182,780, respectively), and letrozol, an inhibitor of the aromatase responsible for converting androgen ... and then to estradiol by the P450 aromatase. It has been shown that approximately 35% of cervical carcinomas express aromatase [37] and that DHEA binds to the androgen receptor and estrogen receptors ... R (2005) Dehydroepiandrosterone inhibits the prolifera- tion of HUVEC by enhancing the expression of p53 and p21, restricting the phosphorylation of RB, and is androgen- and estrogen -receptor independent....

Ngày tải lên: 16/03/2014, 00:20

... Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia coli transthyretin-related protein Erik Lundberg 1 , Anders Olofsson 2 , Gunilla T. Westermark 3 and ... tetrameric and monomeric structures. Thermostability and protein structures We have analyzed the structures of hTTR (Protein Data Bank code: 1F41 [12]), sbTTR (Protein Data Bank code: 1SN2, [16]) and ... C, and D, of the tetrameric structures were: hTTR, A10–A122, B10–B122, A¢11– A¢122, and B¢10–B¢122; sbTTR, A10–A122, B10–B122, C11–C122, and D10–D122; and ecTRP, A4–A114, B4–B114, C5–C114, and D4–D114. c The...

Ngày tải lên: 16/03/2014, 01:20

... a-helices and 12% b-turns (Fig. 1). Incubation of the protein with dif- ferent ligands such as palmitate, caprylic and arachi- donic acids and retinol in concentrations sufficient to saturate the protein ... ethanol and embedded in paraffin. For R. Jordanova et al. Conformation, ligand binding and distribution of nematode protein Ag-NPA-1 FEBS Journal 272 (2005) 180–189 ª 2004 FEBS 187 Conformational and ... nonpolar and com- pletely isolated from the solvent. Here we analyze the conformational and functional properties of Ag-NPA-1 as well as the tissue and cellular distribution of the protein. The...

Ngày tải lên: 16/03/2014, 18:20

... extracts a single band was recog- nized at  43 kDa indicating the presence of the full- length protein, in mitochondria three bands at about 42, 43 and 44 kDa were detected, while no bands were visible ... could assume that in mitochondria the unprocessed protein, the mature protein and a post-translationally modified protein might be present. N-terminal sequencing and analysis of peptides by MS could help ... d-CDes protein was fused with the C terminus of the green fluorescent protein (GFP) in the pGFP-C vector the fusion protein remained in the cytoplasm (Fig. 4C). Expression studies on the RNA and protein...

Ngày tải lên: 16/03/2014, 18:20

Ngày tải lên: 17/03/2014, 23:20

Ngày tải lên: 22/03/2014, 17:20

Ngày tải lên: 23/03/2014, 04:20

Ngày tải lên: 23/03/2014, 06:20