anatomy of the human heart game

Ngày tải lên: 08/04/2014, 12:35

Ngày tải lên: 12/05/2014, 16:54

... all of the OLP epithelium. The count was performed by two investigators without knowledge of whether the samples were control or experimental. The total number of basal and suprabasal epithelial ... Negative controls consisted of omission of the primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit serum instead of the primary antibody. Cell quantification ... OLP would require an increased immunolocalization of hMSH2. Therefore, the reduced expression of hMSH2 protein could make the epithelium of OLP more susceptible to DNA mutation, making it prone...

Ngày tải lên: 03/11/2012, 09:54

Ngày tải lên: 12/12/2013, 00:03

Ngày tải lên: 18/12/2013, 21:45

... respectively, of that of HPRT. This low activity could be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... spectrophotometric methods. Because the enzymatic activity of PRTFDC1 towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism ... activity [10]. The structural characterization of numerous com- plexes of the human HPRT and several bacterial and protozoan HPRTs have been undertaken [13–17]. The structure of human HPRT can...

Ngày tải lên: 15/02/2014, 01:20

... per monomer of poly(dT), showing that the changes were mainly due to the binding of the first DNA, while the binding of the second DNA had less influence (Fig. 5A). To ensure that the estimation of the ... by mutations on the DNA-binding site of Rad51, suggest- ing that the L2 loop of Rad51 is not far from the DNA-binding site. Consistent with this idea, the fluor- escence of the tryptophan inserted in the ... 1). These results confirm that the residues are close to the DNA-binding site. We then examined if these fluorescence changes occurred by the binding of the first or second DNA, by titrating these...

Ngày tải lên: 19/02/2014, 06:20

... encoded another type of KLK11, isoform 2, despite the presence of exon 1c. The secretory path- ways of KLK11 isoforms appeared to differ, although both isoforms were secreted from cells. Hence, the ... show the transcription initiation sites of the human and a white arrowhead shows the transcription initiation site of the mouse. Dashes show gaps and asterisks show the same nucleotide in the ... mechan- ism of KLK11 gene expression. Results Three different primary transcripts are derived from the human KLK11 gene We determined the nucleotide sequences of 15 clones of the PCR products from the human...

Ngày tải lên: 19/02/2014, 06:20

... and phylogeny. How- ever, the nature of the connections between these factors needs to be understood at the level of the pro- tein domain. The global properties of the HTFN topology are partially ... property of the bHLH domains. Therefore, this seems to be a topo- logical constrain derived from the evolution of this family. Evolution based on domain reusing might explain the abundance of certain ... TFIIE-a, TFIIE-b F 57 42% Zn finger domains. It contains the 90% of the members of nuclear receptor superfamily (they are Zn fingers also) of the HTFN. 14-3-3 zeta, STAT1a, STAT1b, dCREB, ATF-1, FTF,...

Ngày tải lên: 19/02/2014, 07:20

... cells. The positions of the molecular size markers (kDa) are indicated to the left and right of the (C) and (D), respectively, whilst the position of the Oct-1 (98 kDa approximately) and the two ... (2002) Characteri- zation of the 5¢ untranslated region of alpha and beta isoforms of the human thromboxane A2 receptor (TP). Differential promoter utilization by the TP isoforms. Eur J Biochem 269, ... (Prm)3 within the human TXA 2 receptor (TP) gene that directs expression of TPb in HEL92.1.7 and HEK293 cells [25]. A schematic of the human TP gene highlight- ing the positions of the previously...

Ngày tải lên: 19/02/2014, 16:20

... to the protein of interest. In the case of the rpL32 fragment or the (GCC) 9 sequence, there are several repeated ZF5-binding sites within a single oligonucleotide. Therefore, the affinity of ... (1994) Identification of a GC element in the promotor of the murine L32 ribosomal protein L32 gene and the 3¢-ter- minal region of the human apolipoprotein A-I gene, capable of binding with murine and human ... Institute of Influenza, Ministry of Health of the Russian Federation. The first pair of primers (5¢-GGCCTTCAAGGCATTAAG-3¢,5¢-AAACAAATGG CCTGTCCG-3¢) spanned a 958 bp 5¢-part of the ZF5 coding region; the...

Ngày tải lên: 07/03/2014, 05:20

... epitopes located in the MUC5AC C-terminal cysteine-rich part. Further localization of the epitopes of 45M1, 2-12 M1 and 166M1 In order to further locate the epitopes of the hitherto unmapped 45M1, ... detailed map of the epitopes for the mAbs, where 45M1 recog- nizes an epitope located in the N-terminal region of the C-terminal cysteine-rich part of MUC5AC, pre- sumably in the last CysD domain of the ... 5. Mapping of the 45M1, 166M1 and 2-12M1 epitopes on the C-terminal cysteine-rich part of human MUC5AC. The upper part of the figure shows a schematic representation of full-length human MUC5AC...

Ngày tải lên: 07/03/2014, 05:20

... subunit of Pph3p required for the binding of Psy2p, we examined the sensitivity of the YBL046w ⁄ Psy4p deletion strain to cisplatin. The comparable increased sensitivities to cisplatin of the homozygous ... was the same as that of the control strain transformed with the vector YCplac111 (data not shown). The YBL046w gene was therefore termed PSY4 (for platinum sensitivity 4). The primary target of ... Superose 6 at the same anomalous position of  450 kDa as Dro- sophila His 6 –R2 (Fig. 3) and human His 6 –R2 [16]. Examination of the phenotype of S. cerevisiae strains carrying a deletion of the YBL046w...

Ngày tải lên: 07/03/2014, 12:20

... per- centages of the cells in the G0 ⁄ G1, S and G2 ⁄ M phases of the cell cycle after treatment with 20 l M or 100 lM ATP for 24 h. The gat- ing used for the quantification of the cells in the G0 ⁄ ... completely reversed the inhibition of LXF-289 cell proliferation by ATP. Further indirect support for the involvement of ERK1 ⁄ 2 is derived from the observed translocation of ERK1 ⁄ 2 to the nucleus. The translocation ... detectable decrease of the p50 form of NF-jB1 in the cytosol after 10 min, which is still visible after 60 min (Fig. 5D). Simultaneously with the decrease of p50 in the cytosol, an increase of NF-jB1 p50 in the...

Ngày tải lên: 07/03/2014, 12:20

... late steps of the process. For example, early in the NER process, RPA assists TFIIH in the opening of the DNA helix around the damage site [36]. Further- more, in the presence of the XPA minimal ... sites. Taken together, these findings suggest that the struc- tural properties of DNA-damaged substrates, whether intrinsic or the result of protein binding, function in the recruitment of the XPC–hHR23B ... Binding of XPG induces the release of XPC–hHR23B, whereas XPF–ERCC1 triggers exci- sion of the damaged DNA and the release of XPA and TFIIH. Subsequently, the newly formed gap in the DNA is filled by...

Ngày tải lên: 07/03/2014, 12:20

... in front of the HSVtk promoter and transfected into HeLa cells. CAT activity was normalized for transfection efficiency. The values of the activities are set relative to the activity of the clone ... mL of sample. The column was eluted at 0.1 mLÆmin )1 . Fractions of 0.5 mL were collected after the void volume of the column. SDS/PAGE and protein identification by MALDI-TOF MS Samples from the ... identifica- tion of NF1 by MALDI-TOF MS is based primarily on peptide mass matches within the conserved DNA binding domain, we cannot distinguish between the various isoforms. Furthermore, NF1 exist in the...

Ngày tải lên: 07/03/2014, 15:20

... localization in the ER [19]. We found that the TMD of UGT1A pro- teins appended to the C-terminus of the ectodomain of CD4 plasma membrane glycoprotein was able to retain the chimeric protein in the ER ... that disruption of the dilysine motif KSKTH of UGT1A by mutation of lysine to serine residues or by extending the length of the cytoplasmic tail to relocate the dilysine from the crit- ical positions ... together, these data indicated that the TMD domain of UGT1A was sufficient to retain the ectodomain of CD4 protein in the ER. Because the carbohydrate moieties of proteins that are trans- ported to the...

Ngày tải lên: 07/03/2014, 16:20