analysis of gene expression in bone by quantitative rt pcr

... Effects of n-3 and n-6 PUFAs on the cyclin D1 gene promoter We studied the mechanism(s) underlying the inhibition of cyclin D1 gene expression by EPA and DHA by examining the effects of n-3 and ... DHA could exert antiproliferative effects by interfering with Ras/Raf pathways Inhibitory effects of n-3 PUFAs on cyclin D1 gene expression and inhibition of IL-1b-induced Egr-1 gene activation ... autoradiogram of three independent semiquantitative RT- PCR experiments, whereas the histogram shown in the lower part of the figure represents data from real-time quantitative RT- PCR analysis Semiquantitative...

Ngày tải lên: 19/02/2014, 16:20

... decomposition of the RNA Determination of the level of BACE mRNA and ribozyme RNA expression in mammalian cells The level of BACE mRNA and ribozyme RNA was monitored by RT/ PCR using a OneStep RT/ PCR kit ... level of BACE protein, and in consequence, to reduce the amount of Ab peptide released in neuronal cells We asked the question whether we can inhibit expression of the gene of BACE protein using ... used in RT/ PCR At postincubation time h (12 h after the beginning of transfection, see Materials and methods) only a minimal amount of ribozyme RNA was present in the cell lysates The level of...

Ngày tải lên: 08/03/2014, 08:20

... < 0.01) (Fig 1A,B) The inhibition of insulin signalling in the liver is primarily reflected by the attenuation of the insulin-mediated inhibition of gluconeogenic gene expression As FEBS Journal ... with insulin incubation coordinately regulate liver-specific gene expression [42] Their transcriptional regulation, particularly that of PEPCK by insulin, is protein synthesis independent, but involves ... alterations in PEPCK gene expression In this study, we found Foxa2 to be predominantly localized in the nuclei of HepG2 cells incubated with TNFa prior to insulin incubation As reported earlier, insulin...

Ngày tải lên: 16/03/2014, 02:20

... creating plasmid, pRS197; (d) pRS197 was transformed into RAS46 and recombinant strains carrying the chromosomally inserted plasmid (by means of single homologous recombination) were identified by ... containing the anaerobic respiratory chain (Figs and 2) Three other lines of evidence derived from in vivo and in vitro studies of nif regulation further supported our model: first, analysis of ... Gavini, N (1999) Genetic analysis of nif regulatory genes by utilizing the yeast two-hybrid system detected formation of a NifL-NifA complex that is implicated in regulated expression of nif genes...

Ngày tải lên: 17/03/2014, 10:20

... mechanisms involved in the increase or maintenance of bone mass in OA This study of the IT region of the proximal femur in primary hip OA and non-OA postmortem controls investigated changes in gene expression ... findings [24] This indicated an increased extent of both bone formation and bone resorption with age, which together suggest an increase in the rate of bone turnover with ageing in control individuals ... in OA and a decrease in controls [16] This finding is supportive of an increase or maintenance of bone volume in OA individuals versus the agedependent bone loss in the general population [11]...

Ngày tải lên: 09/08/2014, 08:23

... hours postinfection At hours post-infection, the level of previously elevated cytokines/chemokines still remained at several folds of induction In contrast to the prominent induction of cytokines/chemokines ... differences in the profiles of cytokines/chemokines induced by influenza viruses of different pathogenicity H5N1 was a more potent inducer of inflammatory cytokines/chemokines; particularly TNF-a, CXCL-10/IP-10, ... prominent during the early phase of infection with H5N1/2004 and H9N2/1997 viruses than those induced by H1N1/2002 virus At the late phase of H5N1 infection, more intense induction of cytokine/chemokine...

Ngày tải lên: 12/08/2014, 02:20

... protein-coding genes, with 6,117 genes remaining after filtering dubious open reading frames (classified by the Saccharomyces Genome Database; Additional data file 8) In the case of RA data, as in ... overall branching pattern of the tree generated was characterized by two large groups: in one of them (clusters 1-8) most clusters showed profiles in which rates (points and in the profile) were ... proteins were one of the most uniform groups (Figures and 4) Nevertheless, as shown also in Figure 3, six genes encoding proteins of this group showed a variant profile characterized by an inversion...

Ngày tải lên: 14/08/2014, 08:20

... by in situ hybridization Cytogenet Cell Genet 39, 220-223 Lefevre F & Labonnardi6re C (1986) Molecular cloning and sequencing of a gene incoding biologically active porcine interferon alpha J Interferon ... orientation of close genes (Morton et al., 1984), The - - - bridging the gap between the physical and the genetic maps, of genes close enough to be considered as genetically linked - by the assignment ... 349-360 Lin C.C., Draper P.N & De Braekeleer M (1985) High-resolution chromosomal localization of the 0 -gene of the human ,Q-globin gene complex by in situ hybridization Cytogenet Cell Genet 39,...

Ngày tải lên: 14/08/2014, 19:23

... interleukin gene; λIg: the λ-chain of immunoglobulin gene; γIg: the γ-chain of immunoglobulin gene; IFN-ind: the interferon-inducible gene 6-16; IL4R: the interleukin receptor gene; TAP2: the transport ... of immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible gene 6-16, IL4R – the interleukin receptor gene, TAP2 – the transport protein gene, hnRNP A1 ... lymphomas In this group of upregulated genes there were the a-myb oncogene, the interleukin receptor gene (IL4R), the gene of the transporter protein TAP2, the gene of protease calpain small subunit...

Ngày tải lên: 02/11/2012, 11:08

... cannot determine the global players involved in such complex phenotypes Integration of the gene expression profiling with specific modulation of gene expression in relevant signalling pathway ... expression Genes detected and in following investigated for validation A) an induced expression of the PKN1 gene B) a repressed expression of the ZCD1 gene C) a repressed expression of BSG gene It ... regulated genes were detected after processing and analyzing with Illumina’s BeadStudio Data Analysis Software and are described in the following part according the definitions of the Illumina’s...

Ngày tải lên: 03/11/2012, 11:44

... next determined gene expression in different adipose depots in response to PM2.5 exposure, in terms of the expression of BAT-specific and WAT-specific gene profiles by real-time PCR analysis UCP1 ... determined by counting the number of positive stained nuclei in 10 random fields Quantitative real-time PCR Total RNA was isolated using TRIzol reagent as instructed by the manufacturer (Invitrogen, ... also increased in mBAT in response to PM2.5 exposure We did not observe significant differences in the gene expressions of Hoxc9 or Igfbp3 in the WAT, neither was the gene expression of Dpt in...

Ngày tải lên: 06/03/2014, 19:20

... PDK mRNA expression, selective inhibitors of signalling pathways known to be activated by insulin were used Two distinct phosphatidylinositol 3-kinase (PtdIns3K) inhibitors, wortmannin and 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyan-4-one ... selectively inhibited by the mitogen-activated protein kinase kinase (MEK) inhibitor, U0126 [32,33] Therefore, the role of the MAPK pathway in regulating PDK mRNA expression was investigated by incubating ... regulated independently by the main fuel sources in muscle and by insulin through directly altering the expression of the human PDK2 and PDK4 isoforms In addition to our findings, insulin has been shown...

Ngày tải lên: 07/03/2014, 17:20

... protein response genes in Arabidopsis vesicle trafficking ⁄ transport, vacuolar protein sorting, cell wall biogenesis, and lipid ⁄ inositol metabolism In comparison with those of yeast, the UPR genes ... genes of the  27 000 proteincoding genes of Arabidopsis with an Affimetrix GeneChips [32] In this paper, we present a list of the UPR genes of Arabidopsis identified among all the protein-coding ... confirmed by real-time RT- PCR analysis (Fig 3) In addition, we found that an antiapoptosis protein, Bax inhibitor-1 (BI-1) [35,36], was also included in the list of up-regulated UPR genes Induction of...

Ngày tải lên: 07/03/2014, 21:20

... intron of the ANX4 gene in OVTOKO and OVISE cells but not in MCAS cells, indicating that endogenous p53 protein directly binds to the ANX4 gene in CCA cells To verify the involvement of p53 in ... p53 binding To examine whether endogenous p53 actually binds to these regions in CCA cells, we performed chromatin immunoprecipitation (ChIP) assays using PCR analysis of the p53 binding domains ... Comprehensive gene expression profiling has revealed that the pattern of gene expression in CCA cells is clearly distinct from that of other EOC cells [11,12] In particular, the annexin IV (annexin A4,...

Ngày tải lên: 22/03/2014, 16:20

... differences in expression of genes encoding enzymes and other proteins involved in intermediary metabolism Perivenous expression is indicated by green, and genes with preferential periportal expression ... FEBS A Braeuning et al Zonal gene expression in mouse liver separation of hepatocytes into periportal and perivenous subfractions was determined by real-time RT- PCR analysis of GS expression and ... Zonal gene expression in mouse liver A Braeuning et al enzymes of serine ⁄ glycine metabolism: Glycine decarboxylase, a component of the glycine dehydrogenase complex that converts glycine to serine,...

Ngày tải lên: 23/03/2014, 10:20

... the expression of ecdysone receptor B1 isoform in imaginal wing cells of P interpunctella at the last larval instar In the fifth larval instar of M sexta, it has been reported in vitro that 20E induced ... isolation, DNA minipreps were prepared and correct insertion was determined by restriction enzyme analysis The DNA clone containing the proper insert was sequenced by the dideoxy chain termination method ... results indicate that PIEcR is a B1 type isoform Effect of 20E and anisomycin on induction of PIEcR-B1 mRNA Using a PIEcR-B1 specific probe located in the N-terminal region of A/B domain, the Northern...

Ngày tải lên: 23/03/2014, 13:20

... cytokinins and hormones The main purpose of a regulated gene expression system is to control the timing and levels of transgene expression in vivo Whether the expressed protein remains within the ... Ras-related GTP-binding proteins that are localized in distinct intracellular compartments [24] Mutations in the Rab gene can alter the morphology of entire organelles by blocking protein transport along ... expression of host genes AMK, RG-121150, affects gene expression in 293 cells The affect of the AMK RG-121150 on gene expression in 293 cells was analyzed using microarray and qRTPCR In microarray analysis, ...

Ngày tải lên: 30/03/2014, 03:20

... predictive values of a panel of miRs by quantitative realtime PCR in formalin-fixed paraffin-embedded tumor specimens from 639 resected NSCLC patients participating in the International Adjuvant ... now being exploited in cancer, attempting to modulate their expression, reintroducing microRNAs lost in cancer, or inhibiting oncogenic microRNAs by using anti-micro oligonucleotides [38] In a ... Hospital of North Norway, Tromso, Norway 2Institute of Clinical Medicine, University of Tromso, Tromso, Norway 3Department of Pathology, University Hospital of North Norway, Tromso, Norway 4Institute...

Ngày tải lên: 18/06/2014, 16:20

... area of the condyle The areas of India ink staining were outlined using a permanent marker Tracings of the India ink-stained tibial and femoral condyles were evaluated without knowledge of dog ... assessments The lamina splendens was not disrupted in any location based on lack of India ink staining, indicat- ing that AC surface integrity was maintained for two weeks in the dogs in this study ... Hydroxyproline content of cartilage by region Hydroxyproline content of cartilage by region The HP content of each cartilage region from the ACL-X joint was compared to the corresponding region in the...

Ngày tải lên: 20/06/2014, 00:20