analysis of dnase i hypersensitivity sites in the cd3g cd3d gene region
Báo cáo khoa học: Evaluation of potential regulatory elements identi®ed as DNase I hypersensitive sites in the CFTR gene doc
Ngày tải lên :
08/03/2014, 10:20
... further DHS in airway epithelial cells were investigated further in the airway carcinoma cell line Calu3 Of particular interest were the DHS in introns and 18, which were strongly evident in ... limitations of transient transfection assays, further in vivo analysis will be required to evaluate the role of the intron 20 and 21 DHS in CFTR expression in chromatin The DNA sequence within the intragenic ... previously shown that the DHS at 185 + 10 kb in intron of the CFTR gene augments intestinal expression of the gene in vivo, both in human colon carcinoma cells and in transgenic mice [15] It is...
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Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx
Ngày tải lên :
19/02/2014, 16:20
... rectangle Size and position of the HindIII fragment hybridizable with the probe is shown below the map Exons and introns are indicated as in Fig (B) Genomic Southern hybridization HindIII digested ... the region involved in DNase I general sensitivity or of the region for the formation of DHSs, although chromatin opening activity of the LCR has been described in several loci using transgenic ... (R-PCR) [16] The regions with active histone modifications were again sensitive to DNase I To determine the in vivo role of DHSs in B cell-specific transcription of the Ig-b gene, genetic studies such...
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Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt
Ngày tải lên :
07/03/2014, 02:20
... accumulation for the latter, indicating that in the absence of an N-terminal domain distinct localization is lacking Finally, the Lsm1p Sm domain by itself (LsmDN1DCp) accumulates in both the nucleus ... this raises the interesting possibility that the Lsm1p and Lsm8p N-termini prevent aggregation of the Lsm complexes, potentially by interacting with the prionlike C-terminal domain of Lsm4p This ... the exception of LsmDN88p This suggests that each of the Lsm8p domains contributes to proper U6 binding, either directly or indirectly, by affecting the RNA-binding ability of the resulting heteroheptameric...
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An Analysis of Business Administration Students Interest in the area of production and Operations pot
Ngày tải lên :
23/03/2014, 05:22
... the analysis The fifth and final dimension involved an assessment of the ‘perception of the necessity’ including the area of Business Administration course curricula The evaluation of this dimension ... area, while the interested ones, despite pointing out high interest in the other constructs, have little interest in moving to this area in their professional career Final considerations For this ... organizational activities Through the model itself, it is possible to insert what is most relevant and innovative in administrative thought and practice, maintaining a model in which theoretical...
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Báo cáo y học: "Analysis of immunoglobulin light chain rearrangements in the salivary gland and blood of a patient with Sjögren’s syndrome" pptx
Ngày tải lên :
09/08/2014, 03:24
... 0.005) Further analysis of the distribution of individual Vκ genes in the productive Vκ gene repertoire revealed a significantly higher frequency of the gene A27 in the parotid gland of the patient ... accumulate in the parotid gland but cannot clarify the origin of these cells Whatever the primary aberration in the induction of the salivary inflammation, one abnormality relates to the generation of ... be involved in the pathogenesis of SS [2], could thus also be involved in the selective processes shaping the VL gene repertoires of B cells accumulating in the parotid gland of this SS patient...
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báo cáo khoa học: "Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin" pot
Ngày tải lên :
11/08/2014, 11:21
... (Additional file 11) The eight cystenyl residues forming disulfide bridges in fungal GH45 enzymes and maintaining their folded structure [16] were conserved in the expansin domain of some of the ... residue instead of an alanine residue in the GGACGY motif of the GH45 domain As expansins disrupt noncovalent bonding between cellulose microfibrils and matrix glucans that stick to the microfibril ... complete divergence of the plant expansin families within the embryophytic lineage Distinct differences in gene architecture between Micrasterias and embryophytic expansins have raised the question...
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Báo cáo y học: " Molecular analysis of HBV genotypes and subgenotypes in the Central-East region of Tunisia" potx
Ngày tải lên :
12/08/2014, 02:20
... Monastir, Tunisia Authors’ contributions NH and OB conceived of the study, participated in its design, and in drafting the manuscript HT and JB participated in the study design and coordination ... Discussion Our study, conducted for the first time in a population from the Central-East of Tunisia, identified genotype D as the most prevalent in this region These findings are concordant with ... - Discordant results are shaded Figure Phylogenetic analysis based on 86 sequences of 431 nucleotides within the HBsAg region The evolutionary history was inferred using the Neighbor-Joining...
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Báo cáo y học: " Analysis of repetitive DNA distribution patterns in the Tribolium castaneum genome" pptx
Ngày tải lên :
14/08/2014, 08:21
... make valid comparisons, analysis of these regions indicates a noticeable reduction in the rate of recombination in regions containing a high density of repetitive DNA, supporting our hypothesis that ... heterochromatin and euchromatin in Tribolium is much lower than Table Recombination rate as reflected in physical size of recombination units in putative heterochromatin and euchromatin in the Tribolium ... heterochromatin In insects, large blocks of pericentric heterochromatin have been identified by C-banding In tenebrionid beetles, including Tribolium, large blocks of pericentric heterochromatin often...
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Analysis of p13k independent survival pathways in the prostate cancer cell line LN cap 1
Ngày tải lên :
12/09/2015, 08:18
... serum-mediated survival is MEK-dependent .99 EGF inhibits LY-induced apoptosis via inactivation of Bad 106 Serum-mediated inhibition of LY-induced cell death is independent of Bad inactivation ... cytoplasmic region The phosphorylated tyrosine residues act as docking sites, allowing other intracellular proteins with phosphotyrosinebinding domains to physically associate with the RTKs Association ... Src family tyrosine kinase (Marais et al 1997) However, several findings of Raf activations still remains controversial including the role of 14-3-3 as other studies indicate that binding of 14-3-3...
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Analysis of p13k independent survival pathways in the prostate cancer cell line LN cap 2
Ngày tải lên :
12/09/2015, 08:18
... remains possible that incomplete silencing of Bad could be the cause of partial inhibition of cell death, nevertheless it also implicates involvement of other apoptosis-inducing factors This is in ... complete inhibition of apoptosis, and dual silencing of Bad and Bax did not provide further inhibition These findings support the current model of interaction among members of the Bcl-2 family in the ... Interestingly, incubation with EGFR kinase inhibitor prevented the internalization and degradation of the receptor It is possible that the kinase activity of EGFR of may play a role in the degradation...
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Molecular analysis of maternal diabetes induced changes in the developing neural tube
Ngày tải lên :
26/11/2015, 12:46
... diabetic TGF-ß l TGF-ß1 5A tm htms III III A B htm III TGF-ß l sn III TGF-ß l hi dn chp bt C D TGF-ß l TGF-ß l III III E F TNF-α III G Fig 17 TNF-α G III H Lectin TGF-ß1 A B C Lectin TNF-α III D Fig ... B III III A B ctx LV LV LV LV cn C D III III III E F III III sn hi dn G Fig 15 H chp bt number of microglia (/mm ) 45 40 35 30 25 20 15 10 Fig 16 ** Normal Diabetic CP-treated CP-treated diabetic ... 40 20 Fig Control Diabetic dc e dc vt A Fig vt B e A Fold of induction 3.5 ** 2.5 1.5 0.5 Control Diabetic B 333bp C Fig dc dc III vt A vt B LV A Fig dc e dc III vt vt LV A Fig dt B III e e e...
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Báo cáo y học: "Systematic analysis of genome-wide fitness data in yeast reveals novel gene function and drug action" pot
Ngày tải lên :
09/08/2014, 20:21
... hypothesis is that co-inhibiting compounds share physical targets, suggesting that if the instance compound targets the instance protein, inhibiting the heterozygous deletion strain, then coinhibiting ... calyculin A and wiskostatin The similarity in these profiles resulted from inhibition of strains deleted for genes involved in intracellular transport and multidrug resistance [1] The clusters highlighted ... motivation for including it is the following: in addition to the physical target, genes involved in bioavailability sometimes cause a significant fitness defect when deleted as heterozygotes These...
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Báo cáo y học: " The evaluation of Pat-Pat related injuries in the western black sea region of Turkey" ppsx
Ngày tải lên :
13/08/2014, 23:20
... explained by the high density of young population living in our region and the presence of male-dominated society from a sociological perspective With the region s main income source being agriculture, ... hospitalization indication has increased this Page of rate due to most of the population living in our region residing in rural areas away from the city center, which not have medical facilities ... young individuals play an active economical role, earning an income, not letting the elderly work, thus increasing the probability of accidents related to agricultural occupations and explaining the...
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Báo cáo y học: " Patterns of expansion and expression divergence in the plant polygalacturonase gene family" ppsx
Ngày tải lên :
14/08/2014, 17:22
... Biology 2006, (b) Volume 7, Issue 9, Article R87 (c) Kim et al R87.13 (d) comment 10 (e) ii iii iv v i ii iii iv (g) (f) i ii iii iv v i v ii iii iv v reviews i (h) reports ii iii iv v ii iii ... At3g62210 Figure Collinearity of PGs in AGI block 23a Collinearity of PGs in AGI block 23a After locating areas with similarities in the block 23a (see also Additional data file 4), six distinct PG-containing ... contain PGs in the corresponding duplicated regions are shown in Additional data file After identifying the collinear regions surrounding PGs, we took at least 100 kb regions surrounding PGs and their...
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An analysis of nouns formed by suffixes in english a case study of the textbook solutions pre intermedite
Ngày tải lên :
14/12/2013, 16:45
... “-ce” to the adjective “intelligent” (good at learning, understanding and thinking in a logical way about thing; showing this ability) The consonant “t” in this adjective is dropped “Intelligence” ... think that the text book “Solutions – pre-intermediate” is normal and 30% of them think that it is difficult to their students It is the reason why their students find confusion in using this ... activities to help students improve their English skills It supplies many skills such as listening skill, speaking skill, reading skill and writing skill Each unit contains some reading texts with...
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Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf
Ngày tải lên :
14/02/2014, 18:20
... existing within inactive loci In the case of the lysozyme locus, which was initially used to help establish the active domain model, it was later found that its domain encompasses the ubiquitously ... cooperativity in the binding of transcription factors to nucleosomes [238–240] Even in the absence of protein–protein interactions, factors can assist each other’s binding to DNA within nucleosomes ... transcribed regions of active genes in human T cells [124] In a study in yeast, mutations were introduced alone or in combination in lysines 5, 8, 12 and 16 in the gene for histone H4 [125] Of these, the...
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Báo cáo khoa học: Identification of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot
Ngày tải lên :
07/03/2014, 12:20
... emerin, like other INM proteins, binds to lamins It is linked to EDMD by its interaction with lamin A [20] In EDMD this interaction is weakened or lost either by mutations in emerin itself, which ... stable isotope labeling with amino acids in cell culture (SILAC) in combination with antiphosphotyrosine immunoprecipitation and tryptic in- gel digestion to identify human emerin phosphorylation sites ... proteins nesprin 1a and nesprin [28,29] Since the different binding regions overlap at least partially, a simultaneous binding of some binding partners may be excluded, giving rise to distinct...
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A comparative analysis of methods to represent uncertainly in estimating the cost of constructing wastew
Ngày tải lên :
15/03/2014, 23:17
... 22Á70 60Á67 28Á15 42Á96 II II III III III III III III III II III II III III III III III III II III III II III II II Treatment process Ic IId IIIe a a a a a a a EQ CS AS RBC VIP A /O OD COa SFa ... 103Á70 74Á07 66Á63 40Á00 113Á33 440Á41 5Á64 215Á26 102Á13 267Á04 II II III I II II II II III II II II III III II I II II I II I I Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â ... 13Á5 29Á6 II III 6Á89 54Á8 II 3Á91 4Á46 48Á48 51Á9 III III 3Á01 4Á81 12Á33 35Á2 92Á6 42Á7 II II III 9Á19 14Á22 9Á33 11Á81 15Á15 13Á93 66Á7 55Á3 ±b 60Á4 92Á5 103Á7 I III III III III III 7Á00 10Á93...
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Báo cáo khoa học: Co-operation of domain-binding and calcium-binding sites in the activation of gelsolin pptx
Ngày tải lên :
31/03/2014, 01:20
... purple, respectively sequence 203–225, including one of the actin-binding sites, is in interaction with the C-terminal a-helix of G6 domain The sequence 159–193, including the second actin interface, ... which is observed for the binding of calcium to G2 domain Footprint of G2 on gelsolin G4–6 domain Two sites within the G2 domain appear involved in the G2- G4–6 interface in the presence of EGTA ... binds to G4–6 with high affinity (Kd ¼ 0.5–0.8 lM) in EGTA, weaker binding was evident in the presence of mM calcium (Fig 5) In addition a change in the interface occurs during calcium binding...
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Analysis of socio-economic factors affecting technical efficiency of small-holder coffee farming in the Krong Ana Watershed, Dak Lak Province, Vietnam
Ngày tải lên :
12/06/2014, 15:01
... objectives were to (i) identify the factors affecting coffee production, (ii) estimate the technical efficiency of coffee farming and (iii) identify factors contributing to technical inefficiency ... 2007) Another description of technical efficiency includes the ability to minimize input use in the production of a given output vector, or the ability to obtain maximum output from a given input ... Improvements in both quantity and quality of extension services may increase technical efficiency of coffee production for farmers in the Cu Kuin district More in- depth investigation into population policies...
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