analysis of assembly of secreted mucins

... subunits of the yeast bc1 complex indicated on the left side of the blots Ó FEBS 2004 Assembly of the yeast cytochrome bc1 complex (Eur J Biochem 271) 1213 Table Cytochrome bc1 subunit analysis of ... copy of subunit results from loss of assembly of the cytochrome bc1 complex and can be suppressed by overexpression of subunit J Biol Chem 266, 14958–14963 25 Laemmli, U.K (1970) Cleavage of structural ... Electrophoretic analysis of DNA on agarose gels, restriction endonuclease analysis, ligation of DNA fragments, Ó FEBS 2004 1212 V Zara et al (Eur J Biochem 271) transformation of Escherichia coli...

Ngày tải lên: 19/02/2014, 12:20

... SPR analysis of the RuvAC–Holliday junction ternary complex To further examine the hypothesis that a tetramer of RuvA can bind to each face of a Holliday junction, the effect of the addition of ... removal of RuvA from DNA can be affected by addition of M salt, highlighting the role of electrostatic interactions in the binding of RuvA to the junction (data not shown) The binding of RuvA ... magnitude of protein concentration until 100% of the binding sites are occupied However, the RuvA–Holliday junction binding occurs over two orders of magnitude of RuvA concentration until 100% of the...

Ngày tải lên: 21/02/2014, 01:21

... gene encoding the secreted CM from Yersinia pestis (*YpCM, y2828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure analysis of the protein Results ... conducted a brief study of the recombinant MtCM Our work is aimed at the further characterization of MtCM The true primary sequence of MtCM is complicated by virtue of a number of presumptive in-frame ... (16%) of the production and purification of 105-MtCM Lane 1: uninduced cell-free extract of E coli BL21(DE3) harboring the pG58–105-MtCM clone (25 lg of protein) Lane 2: induced cell-free extract of...

Ngày tải lên: 17/03/2014, 17:20

... of health care provider; (2) offer or provide coverage for the screening, diagnosis, or treatment of a particular disease or condition; or (3) offer or provide coverage of a particular type of ... illustration of what could happen as a result of the passage of the bill, not a projection of what will happen The impact of AB 1774 on utilization could vary substantially, depending on a number of factors ... provides an analysis of the medical, financial, and public health impacts of Assembly Bill 1774, a bill to mandate the coverage of screening and diagnostic tests for the purpose of assisting or facilitating...

Ngày tải lên: 22/03/2014, 10:20

... full-length inserts [45], allowing analysis of amino-terminal signal peptides to be carried out The evolutionary history of secreted immunomodulators is likely to be that of recent adaptation from ancestral ... Strongylida are members of the Rhabditina, including C elegans, and both are grouped in Clade V of the Nematoda, on the basis of small subunit rRNA sequence analysis [48] The set of 742 clusters was ... using a cutoff score of 80 in BLASTX (P < e-10) The number of ESTs bearing potential signal sequences was then calculated and the results are shown here (b) Effects of relaxing cutoff scores...

Ngày tải lên: 09/08/2014, 20:20

... may clarify the origin of duplicated transcripts in the genome assembly BAC assembly The quality of these BAC assemblies is critical for a valid assessment of the genome assembly and the automatic ... of an unusually high percentage of genomic blocks experimentally shown to contain single-copy sequences (57% (8 of 14)), indicates the presence of an assembly issue which affects the number of ... number of repeats identified An initial analysis of in silico gene annotations derived from the masked sequences revealed that a number of transposons were not identified as a result of the incomplete...

Ngày tải lên: 14/08/2014, 07:21

... ii Table of Content ABSTRACT .I TABLE OF CONTENT II LIST OF ILLUSTRATIONS VI LIST OF TABLES VIII LIST OF EQUATIONS X LIST OF ABBREVIATIONS ... Operations of section I Body - Subassembly (part 3/4) 32 Table 5-7 Operations of section I Body - Subassembly (part 4/4) 33 Table 5-8 Operations of section II Collar and Lining - Subassembly ... 5-14 Operations of section IV Final Assembly (part 1/4) 41 Table 5-15 Operations of section IV Final Assembly (part 2/4) 42 Table 5-16 Operations of section IV Final Assembly (part...

Ngày tải lên: 09/05/2016, 21:36

... installation of RM2000 and GP2000 Please always uninstall old program versions before installing a new program release! 1.2 Unit dependency of Import/Export of RM2000 Export and Import of RM2000 ... upgraded or the validity of a time limited version has expired New RM2000/GP2000 users as well as existing RM7 users must get a licence file from Tdv or one of the support offices in order to run ... calculation is numerically extended to cover more exactly “creep of creep” effects ( it is possible to decrease number of time steps ) Extensions of British Standard live load calculation Added possibility...

Ngày tải lên: 06/09/2012, 15:18

... Ownership of the program and the documentation remain with TDV Austria Use of the program and the documentation is restricted to the licensed users Unlicensed use of the program or reproduction of the ... point’ arrow at the top of the screen and the insert symbol Accept the default name Define the Connection Points Click on the intersection point of the CL’s at the bottom of each beam in the position ... load sets and the two loading cases (e.g.: L.C is a UDL of 10kN/m from Elem to Elem 20 and L.C is a Settlement of 0.001m at the beginning of element 1200.) Choose AddCon and insert the ‘Additional...

Ngày tải lên: 06/09/2012, 15:55

... 5-1 MATERIAL OF PRE-STRESSING TENDONS 5-2 DEFINITION OF TENDONS (TENDON PROFILES) 5-4 Creating New Tendon Profiles 5-4 Assignment of Structural Elements ... side of the screen #NODE Definition of nodes and their attributes #ELEMENT Definition of elements and their attributes #TENDON Definition of tendons and their attributes #SPECIAL Comparison of ... re-generate it A typical example of the application of macros is the generation of the Finite Element mesh for the computation of the cross-section properties of cross-sections with standard shapes...

Ngày tải lên: 06/09/2012, 15:55

... because of activity against key pathogens Consideration of the influence of appropriateness of empiric therapy against subsequently identified pathogens was beyond the scope of this economic analysis ... LLC Role of the sponsor SM, AQ, and NK of Johnson and Johnson met authorship criteria by participating in the study design, analysis of results, interpretation of findings, and drafting of the ... Independent statistical analysis The accuracy of the data analysis was independently verified by William Shannon, PhD, Associate Professor of Biostatistics in Medicine, Department of Medicine, Washington...

Ngày tải lên: 25/10/2012, 10:02

... cut-off value 15 for strain of –6.5 % a sensitivity of 23 % and specificity of 87 % was calculated for the detection of infarcted segments (AUC 0.6, confidence interval 0.53 – 0.64) A 111 cut-off ... cut-off value of –10.34% resulted in a sensitivity of 54 % and specificity of 59 % (see Table 6) The intra- and inter-observer variation of our data is congruent with the data of Jarnert et al ... (Sre) 0.60 ± 0.35 s-1 The analysis of tissue velocities demonstrated a gradient of systolic (S´) and diastolic (early E´ and late A´) velocities from the apex to the basis of the heart with significant...

Ngày tải lên: 25/10/2012, 11:15

... crux of individualism is the ethnic diversity of various individuals It is not the particular view of the individual that makes them an individualist, but it is the differing views of a group of ... childhood asthma Journal of Asthma 1998; 35: 213–7 Sarafino EP, Gates M, DePaulo D The role of age at asthma diagnosis in the development of triggers of asthma episodes Journal of Psychosom Res, 2001; ... School of Laughter Yoga, described laughter as a “powerful form of exercise that gives you more of a cardiovascular workout than many ‘regular’ aerobic activities (7) Similarly, two stages of laughter...

Ngày tải lên: 26/10/2012, 09:57

... same type of credit This kind of L/C allows the seller to transfer all or part of the proceeds of the original letter of credit to a second beneficiary, usually the ultimate supplier of the goods ... set of rules on the issuance and use of letters of credit The UCP is utilised by bankers and commercial parties in more than 175 countries About 11-15% of international trade utilises letters of ... the beneficiary of the issuance of letter of credit, it just processes the documents and has no direct liability on the letter of credit; besides, it might “confirm” the letter of credit, in which...

Ngày tải lên: 27/10/2012, 16:40

... According to statistics of the IT Industry Department, the US is a big software exporter but its import of software accounts for 30% of the total spending for software of the world and its payment ... because of some difficulties 2.5.1 Scale of operation In general, most software outsourcing companies are of small scale and facing up with dispersed, unprofessional development The scope of software ... 40% of the local enterprises interviewed said they spend money on software application About 20% of software applications by local enterprises are simple, mainly Office software Only 1.1% of the...

Ngày tải lên: 27/10/2012, 16:41

... to the formula: Changes in the level of phosphorylated JNK1&2 (-fold of control) = absorbance of hypoxia-treated cells/absorbance of control cells RT-PCR analysis Total RNA was extracted from ... transfection of Bag-1 Morpho/AS to JAR cells did not alter the expression of PTEN after the exposure to hypoxia, which would mean that the expression of PTEN, and also that of Bcl-X, was independent of ... expression profiles of proteins involved in the mitochondrial (bcl-2, bax, and bcl-x, etc.) and stress reaction-related pathways of apoptosis (Fig 2) Table Altered protein expression of JAR cells...

Ngày tải lên: 31/10/2012, 15:12

... with a SLR of 3.30, 2.61, 1.68 and 1.52, respectively ANK3 was the only significantly down-regulated gene with a SLR of -5.02 Figure Hierarchical clustering analysis of gene expression profile in ... concentrations of ethanol (100 mM) induced the expression of genes involved in the metabolism of ethanol [16] In addition, the metabolism of ethanol 33 results in an increased production of toxic metabolites ... common expression profiles of genes in various categories demonstrated by cDNA microarray analysis Hepatology 2001;33:676-91 Okabe H, Satoh S, Kato T, et al Genome-wide analysis of gene expression...

Ngày tải lên: 31/10/2012, 15:28

... variants (TV) of exon of the hCDC4 gene Arrows mark the position of the detected mutation in the 5’ UTR of Exon 1-TV3 and the new intronic SNP downstream of exon 10 (B) Chromatograph of the mutation ... exchange of nucleotide (nt) 108 of the exon sequence (Figure 1B) Being located in an untranslated region of the exon, the mutation has no consequence for the hCDC4 protein Sequence analysis of hCDC4 ... the detected mutation in exon Similarly, analysis of samples containing the C/T SNP downstream of exon 10 led to the identification of cleavage products of approximately 101 bp and 444 bp in size...

Ngày tải lên: 31/10/2012, 16:49

... infection was examined qualitatively by immunoblot analysis of sera from patients of both the group Figure shows the IgG immunoblot profile of the sera of patients with deep-seated and superficial ... protein profile which consisted of 25-30 bands with major bands having molecular weights of approximately 120, 92, 80, 66, 55, 42, 36, 29, 24, 20, 18 and 16 kDa (Fig 1) The protein profiles of isolates ... the type of infection [22] In order to compare the whole cell protein profile of S aureus strains associated with deep-seated and superficial infection, whole cell lysates of isolates of both...

Ngày tải lên: 02/11/2012, 11:08

... idea that a non-linear analysis of COP is appropriate These findings also support the suggestion of Blaszczyk and Klonowski [10] that the use of a fractal dimension analysis of COP, particularly ... may provide a good screening tool or measure of disease progression The disadvantage of this type of fractal analysis is that it uses the images of the COP that are then digitised and analysed ... observed [14, 15] The aim of this study was to examine both the effects of age and loss of visual information on control of COP during quiet stance and to evaluate the use of Higuchi’s algorithm...

Ngày tải lên: 03/11/2012, 10:09