analyses of peroxide values and headspace oxygen

... where asj and adj are genetic values of the sire and dam of individual j, and mij is the Mendelian sampling effect of individual j assumed to be independent of the genetic values of the sire and dam ... properties, Bias and MSE, of Bayesian and of EBLUP estimates showed the same pattern over the range of simulated scenarios The bias and MSE of Bayesian estimates were often less than of EBLUP estimates, ... PI (analyses and 10 vs 15 and 16) With missing PI (analyses 11 and 12 vs 17 and 18), the reduction in the MSE of breeding value estimates was more accentuate With full PI, treating CG’s as random...

Ngày tải lên: 09/08/2014, 18:21

... up-regulation of testicular genes during gonad development 77 3.4 Comparative analyses of candidate genes for the identification of early testicular markers in zebrafish 80 3.4.1 Androgen ... 3.4.1.1 Androgen receptor showed sexual dimorphic expression in developing gonads and adult tissues of zebrafish 83 3.4.1.2 Sequence homology and phylogenetic analysis of vertebrate androgen ... 37: Structural organization of Scx1 and alignment of its orthologs…………… 94 Fig 38: Phylogenetic and synteny analysis of Scx1………………………………… 96 Fig 39: Expression analysis of scx1 mouse ortholog by...

Ngày tải lên: 14/09/2015, 14:06

... pumilio, par-1 and pipsqueak) have been identified for the formation of the pole cells Mutations in these genes could result in loss of polar granules, disruption of the pole plasm and lack of germ ... zebrafish as a model have greatly enhanced our understanding of the mode of specification of PGCs, cell-fate maintenance and the migration of these cells towards the gonads, where they differentiate ... and there could be interplay between oogenesis and the activity of the Vasa protein (Ghabrial and Schupbach, 1999) In Canenorhabditis elegans, injection of antisense RNA disrupted oogenesis and...

Ngày tải lên: 08/11/2015, 17:09

... studies of the structure and binding sites of enzymes Inhibitor binding to ribonucleases J Mol Biol 45, 491511 33 Ui N (1971) Isoelectric points and conformation of proteins I Effect of urea ... Table X-ray data collection and renement statistics Rsymm = Rh Ri |I(h) ) Ii(h)| Rh RiIi(h), where Ii(h) and I(h) are the ith and the mean measurements of the intensity of reection h, respectively ... Fc| RhFo, where Fo and Fc are the observed and calculated structure factor amplitudes of reection h, respectively Rfree is equal to Rcryst for a randomly selected 5.0% subset of reections not used...

Ngày tải lên: 14/02/2014, 22:20

... researchers and analysts engaged in analyses of military enlistment behavior This research was sponsored by the Assistant Secretary of the Army (Manpower and Reserve Affairs) and was conducted in RAND ... relative costs of producing the two types of contracts Equity in missioning is important in and of itself and because if recruiters perceive unfairness, it could undermine morale and effort and thereby ... because of pure chance The analogous proportions for stations failing and 12 months in a row are 82 and 23 percent The analyses reported in Chapters Four and Five develop and implement new models and...

Ngày tải lên: 17/02/2014, 22:20

... component command and a number of transportation management officers at bases and stations Many of the business rules documented here first came from these interviews At RAND, we thank John Ausink and Nancy ... programs (and socioeconomic goals) 14 Estimating DoD Transportation Spending: Analyses of Contract and Payment Transactions This work is a direct outgrowth of RAND research on the implementation of ... different types of actions and data entry rules in making transportation purchases This lack of standardization in data systems and procurement processes leads to gaps and overlaps among and between...

Ngày tải lên: 17/02/2014, 23:20

... of the heme pocket of GmHO-1 might be associated with the smaller values of Kazide for GmHO-1 and SynHO-1, and with the ferric character of the heme The Kimidazole values of the three kinds of ... Soret band maxima of the azide-free form at 405 nm and of the azide-bound form at 421 nm, so the relative amounts of the nonbound and azide-bound forms were estimated directly from the values of ... time, indicating the formation of biliverdin In this case, neither bands of the oxy form (at 541 and 578 nm) nor bands of verdoheme and CO–verdoheme (at  690 and at  640 nm, respectively) are...

Ngày tải lên: 19/02/2014, 05:20

... Journalism A Handbook of Values and Practices for the News and Editorial Departments “ Reporters, editors, photographers and all members of the news staff of The New York Times share a common and essential ... perceptions, and so the professional reputations of its staff members Thus The Times and members of its news department and editorial page staff share an interest in avoiding conflicts of interest ... favor,” in the words of Adolph Ochs, our patriarch — and to treat readers, news sources, advertisers and others fairly and openly, and to be seen to be doing so The reputation of The Times rests...

Ngày tải lên: 06/03/2014, 11:20

... 2.88 2.72 3.18 2.84 Od2 of Asp423 OH of Ser425 NH of Ala490 NE1 of Trp664 O3 O6 O7 O7 2.89 2.64 2.94 2.40 NH of Ala490 Od2 of Asp636 Od1 of Asp524a Od2 of Asp524a O7 2.63 OH of Tyr590 NAG3 ()1) NAG4 ... These kcat and Km values were comparable with those of Asp522 mutants (D522N and D522A) In contrast, the D524A mutation affected both kcat and Km values significantly, which were ⁄ 340 and ⁄ of the ... E526A and D524A mutants and refined them to ˚ resolutions of 1.80 and 1.76 A, respectively A summary of crystallographic data collection and refinement statistics is given in Table Superimposition of...

Ngày tải lên: 15/03/2014, 11:20

Ngày tải lên: 16/03/2014, 03:20

... to  30% of the initial rate of UBHY-R, whereas the rates of synthesis of DNA and RNA were 70% and 60% of the initial values, respectively These data suggest that the primary effect of eIF5A ... sites of p414GAL1 and the NdeI–BamHI sites of pET11a The entire ORF of the site-mutated and truncated eIF5A was sequenced for confirmation of the intended mutation or truncation Determination of ... mm NAD, 25 lg of BSA, lCi of [3H]spermidine, 0.1 lg of DHS and 1.0 lg of DOHH and clarified BL21(DE3) lysates containing heIF5As (2–5 lg) After incubation for h at 37 °C, an aliquot of the reaction...

Ngày tải lên: 16/03/2014, 06:20

... mutational rates of the exon of the 3FTx genes are much faster than those of the PLA genes, leading to high variation of KBf-3FTx Cloning and cDNA sequencing Venom glands of only one of the KBf specimens ... present in B candidus venom, while 3FTx-RK is 84% identical to bucain [25] of B candidus Calcium binding and kinetic parameter of the P31-PLAs Four PLAs (Va, Vb-2, VI and II) of KBf and Bf contain ... the prey of Bf and king cobra consists mainly of snakes and reptiles, which are distinct from those of other kraits (e.g B candidus and B multicinctus, which prey on small catfishes, eels and rodents...

Ngày tải lên: 16/03/2014, 12:20

... (1999) Photodissociation of oxygenated forms of the native hemoglobin HbA and its isolated a- and b-subunits and kinetics of molecular oxygen rebinding Dokl Biophys (translation of Dokl Akad Nauk) ... concentrations of 0.1, 0.5, 1.0 and 2.0 m The rate constant of BR and the quantum yield of BR, in the presence of NaCl, gave a direct evidence of significant functional heterogeneity for the a and b subunits ... probability of binding to 6116 Fig Correlations between the values of the association rate constant of BR (k¢) and the quantum yield of BR (c) The data for the a and b subunits within liganded tetrameric...

Ngày tải lên: 16/03/2014, 14:20

... the investigation of activity [27], the creation of antibody chimeras [28], and the study of Table The ratios of the volumes (V) of the models built by SAXS and of the structure of TnC(1–91) (1SKT) ... with excitation at 280 nm and a bandpass of nm, and emission at 320 nm with a bandpass of nm All spectra were baseline corrected with the buffer and were the means of at least three independent ... localization of the N domain of TnC and the first half of the C terminus of TnI The sensitivity to degradation by proteases is related to the conformational flexibility of the protein substrate [31], and...

Ngày tải lên: 16/03/2014, 18:20

... during analyses of the protein at high concentrations The present study describes a very effective method of isolation and purification of PEC and its photoactive a-subunit Crystallization and preliminary ... p.p.m and 9.38 p.p.m., can be attributed to the Z- and E-states, respectively Integration of both pairs of peaks yields the ratios of state populations of Z/E ¼ 12%/88% for the E-state and approximately ... presents the purification and molecular analyses of photoactive a-PEC peptides from phycobilisomes of M laminosus Preliminary results of crystallization and NMR spectroscopy offer reliable information...

Ngày tải lên: 17/03/2014, 10:20

... affinities (Ka) of FabG for its cofactor, NADPH, determined by quenching of the fluorescence of its tryptophan (kmax 334 nm) in the absence and presence of 20 lm ACP, were found to be 40.9 lm)1 and 48.4 ... function of increasing concentrations of NADPH (C) Hill plot of the data obtained as a result of the changes in fluorescence intensity of tryptophan of the enzyme at 334 nm, as a function of NADPH ... residuals for each value of n, for each ligand, to support the given value of n chosen by us for interpretation of our data Also, for a given value of n, the resultant values of association constants...

Ngày tải lên: 23/03/2014, 10:21

... broad Soret band, whereas the Soret bands of the ferrous and ferrous-CO forms were narrow (Fig 1B) The ratios of the intensities of the Soret maximum of the ferric (spectrum aÕ), and ferrous Fig ... ratios of intensities of the Soret maximum of our ferric and ferrous forms to that of the CO-ferrous form were 0.65, and 0.81, respectively Comparison of these ratios to those of Matera et al suggested ... of 0–5 of O2 (B) Spectra were recorded after addition of sodium dithionite (spectrum c, –) to the sample of spectrum b, after addition of CO (spectrum d, - - -) to the sample of spectrum c, and...

Ngày tải lên: 23/03/2014, 21:20

... manner of EGF binding On the other hand, chimeras 4111-1 and 4411-1, generated by the replacement of domain I and of domains I and II, respectively, of ErbB-1 by the corresponding domain(s) of ErbB-4, ... cognate–ligand interaction of ErbB-1 Nevertheless, the 125I-labeled EGF bindings of 4111-1 and 4411-1 were appreciably smaller than those of 1111-1, 1111-4, and 1114-4, indicating that domain I of ErbB-1 ... loaded amounts of the chimeras, shown in a control strip of Fig 3, and thus the NRG1-b1-induced autophosphorylations of 4444-1 and 4441-1 were just slightly stronger than those of 4411-1 and 4111–1...

Ngày tải lên: 24/03/2014, 03:21

Ngày tải lên: 28/03/2014, 15:20

... stability of the enzyme The crystal structures of the ligand-free and shikimate-complexed SeSDH offer incremental knowledge for understanding the catalytic mechanism of SDH, especially the role of the ... (2HK9_D and 2O7S) ternary complexes Analysis of these different conformations reveals the binding information of substrate and cofactor, the structural basis underlying the cofactor specificity of ... analysis of SDH in complex with cofactor, and of even ternary enzyme–cofactor–substrate complexes, shedsnew light on the catalytic mechanism and provides clues for the rational design of anti-infective...

Ngày tải lên: 30/03/2014, 02:20