amp 948 endotoxin gene in maize
báo cáo khoa học: " Gene expression analyses in maize inbreds and hybrids with varying levels of heterosis" ppt
Ngày tải lên :
12/08/2014, 05:20
... hybrid In maize, the different inbred lines have been divided into "heterotic groups" based upon the level of grain yield heterosis [3] Generally, crosses within heterotic groups have lower grain ... study the gene expression profile of hybrid plants relative to the inbred parents [11,14-21] In general, most of these studies have focused on characterizing gene expression patterns in a single ... differences in the expression profiling platforms In this study we have investigated the heterosis and gene expression profiles for a set of maize hybrids with varying levels of parental genetic...
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Báo cáo y học: "Elevated plasma homocysteine is positively associated with age independent of C677T mutation of the methylenetetrahydrofolate reductase gene in selected Egyptian subjects"
Ngày tải lên :
03/11/2012, 09:49
... polymerase chain reaction–amplified fragment in the gene for MTHFR, according to Frosst et al [17] Briefly, about 50 to 80 ng DNA samples were amplified in a final volume of 25 µL containing 1×PCR ... homocysteine levels in the insulin resistance syndrome: the Framingham offspring study Diabetes Care 2001;24(8):1403-10 McCarty MF Insulin secretion as a potential determinant of homocysteine levels ... homocysteine levels in subjects with hyperinsulinemia J Intern Med 2000;247(2):287-94 Fonseca VA, Fink LM, Kern PA Insulin sensitivity and plasma homocysteine concentrations in non-diabetic obese...
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Tài liệu PCR-RFLP ANALYSIS OF BETA-LACTOGLOBULIN GENE IN MURRAH BUFFALOES pdf
Ngày tải lên :
18/02/2014, 02:20
... of intron IV ) and resulted in 262 bp fragment in cattle The same primers were used for amplifying b-lg gene in Murrah buffaloes Genomic DNA Isolation Blood samples collected in a vacuitainer ... Meignanalakshmi and Mahalinga Nainar genotyping beta-lactoglobulin in Sahiwal and Tharparkar catte breeds Milk protein genes might be useful as genetic marker and is a promising alternative to the ... Central cattle breeding farm, Alamadi, Tamilnadu Individual blood samples of ml each were collected from 110 Murrah buffaloes using ml vacuitainer tubes containing EDTA from jugular vein and stored...
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Báo cáo khoa học: Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids docx
Ngày tải lên :
07/03/2014, 15:20
... I, 10 nM insulin, E, lM epinephrine; P, 0.5 mM palmitate; P + I, palmitate + insulin; P + E, palmitate + epinephrine The bars indicate ± S.E.M Values are means of between five and nine independent ... modifies this AMP binding site in a-1 AMPK complexes Alone, epinephrine and palmitate each decreased malonyl-CoA content (Fig 5) Cyclic AMP- dependent protein kinase (PKA) phosphorylates and inactivates ... blocked inactivation of the AMPK by insulin (Fig 7), suggesting a dominance of the fatty acid-driven pathway for activation of AMPK over at least some aspects of insulin signalling This dominance...
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Báo cáo khoa học: Cloning and expression of the first nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss pdf
Ngày tải lên :
07/03/2014, 16:20
... described In an effort to identify immune genes involved in host defence against bacterial infection in rainbow trout (Oncorhynchus mykiss), the gene- expression profile of bacterial-challenged rainbow ... samples using a low cycle number (21 cycles) Trout IL-11 was highly expressed in intestine and gills, and was also detectable in other tissues including spleen, liver, head kidney, brain, skin ... all the tissues examined, with apparent high levels of expression in intestine and gills Intestine and gills are two primary sites where fish encounter foreign insults including infection by bacteria,...
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Báo cáo khoa học: SOX10, in combination with Sp1, regulates the endothelin receptor type B gene in human melanocyte lineage cells pptx
Ngày tải lên :
16/03/2014, 13:20
... simultaneous binding of the two proteins to the GC1 ⁄ CA2 SOX10 and Sp1 bind to the EDNRB promoter in vivo To investigate whether SOX10 and ⁄ or Sp1 bind to the EDNRB gene in vivo, chromatin immunoprecipitaion ... binding of SOX10 and Sp1 proteins to the GC1 ⁄ CA2, but were unable to detect the complexes, containing both SOX10 and Sp1 proteins (data not shown) It is conceivable that the in vitro binding ... SOX10-containing lysates were preincubated for 30 on ice with or without a competitor in the absence of a probe and then incubated with the probe for 30 on ice in 20 lL solution containing mgÆmL)1 bovine...
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Báo cáo khoa học: 17b-Hydroxysteroid dehydrogenase type 11 is a major peroxisome proliferator-activated receptor a-regulated gene in mouse intestine pdf
Ngày tải lên :
16/03/2014, 18:20
... remarkably increased proteins in the intestine by PPARa ligand in total protein mixture of the postnuclear fraction of the mouse intestine 17b-HSD-4 is a multifunctional protein involved in not only inactivation ... (indicated by arrows) in the intestine are shown enlarged (B) Ó FEBS 2004 17b-HSD is a major PPAR a-regulated gene in intestine (Eur J Biochem 271) 4143 more in the intestine than in the liver and ... L-FABP genes is activated by PPARa within a few hours and the mRNAs reach their maximal levels in a day in the liver but not in intestine [13] The levels of their mRNAs in intestine slowly increase...
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Báo cáo Y học: Loss-of-function variants of the human melanocortin-1 receptor gene in melanoma cells define structural determinants of receptor function doc
Ngày tải lên :
17/03/2014, 10:20
... yellow, sulfurcontaining phaeomelanins [17] Although both types of melanins are found in different relative proportions in all skin types, the phaeomelanin/eumelanin ratio is higher in individuals with ... indeed frequent in HMC lines, and that impairment of signaling through the cAMP cascade occurs in these cells even in the absence of mutations in the coding region of the gene Finally, our study ... conserved binding properties but strongly impaired coupling to the cAMP generation system [22] Interestingly, both substitutions are located in the second intracellular loop of the receptor protein,...
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Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf
Ngày tải lên :
17/03/2014, 11:20
... production of recombinant laccase was recently obtained with the Coprinus cinereus laccase gene expressed in A oryzae where results reached from to 135 mgÆL)1 [31] In conclusion, P cinnabarinus laccase ... P cinnabarinus laccase, we expressed the corresponding cDNA in Aspergillus niger, a filamentous fungal host known to overproduce homologous and heterologous proteins of industrial interest In ... pH determination, syringaldazine (17 lM) was also used as the substrate by monitoring the production of colored quinone at 530 nm (e530 ¼ 65 mM)1Æcm)1) [6] Activity is indicated in international...
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Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx
Ngày tải lên :
23/03/2014, 06:20
... the presence of multiple ARE-binding proteins, including stabilizing proteins [29,30] or proteins that promote degradation of mRNAs that contain AREs [31] Recent findings reveal that the RNase L ... cloned into the pCR 2.1 cloning vector (Invitrogen, Paisley, UK), and the isolated clones were sequenced in both directions Amplification and cloning of the Cc RNase gene The Cc RNase gene was amplified ... regulated by binding of the stabilizing HuR protein to ARE sequences located in the 3¢ UTR [32] Alternative polyadenylation is also associated with miRNA targeting, considering that individual miRNA...
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Báo cáo khoa học: Myocyte enhancer factor 2 (MEF2) is a key modulator of the expression of the prothoracicotropic hormone gene in the silkworm, Bombyx mori ppt
Ngày tải lên :
30/03/2014, 20:20
... within the MEF2 consensus binding sequence (Fig 2A, M3) Even at a 100-fold excess, M1 was unable to compete the binding of protein to wt (Fig 2B, lane 5) However, M2 eliminated protein binding ... protein in Bombyx brain bound to the MEF2 consensus binding sequence, and its binding was prevented by an antiserum that recognizes the MADS box of BmMEF2 We examined this further using recombinant ... assay (B) and reporter gene analysis (C) The MEF2 consensus binding sequence in mouse brain [12] is boxed, and the 10 bp MEF2 core binding sequence is shown in capital letters In the gel mobility...
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Báo cáo khoa học: Comparative analysis of the site-specific N-glycosylation of human lactoferrin produced in maize and tobacco plants pdf
Ngày tải lên :
31/03/2014, 07:20
... corn seeds were produced in a greenhouse (T1 to T3 generations) using self-pollinations and cross-pollinations with an untransformed elite inbred maize variety [17] To obtain a greater quantity ... biomass and the ease of extracting protein from grains [34] has led us to initiate the engineering of maize N-glycosylation Acknowledgements This work was supported in part by the University of ... to supplement infant foods In this paper, we report the site-by-site analysis of the maize recombinant lactoferrin (mLf) in comparison with the lactoferrin produced in tobacco (tLf), used as a...
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Báo cáo sinh học: "Detailed genetic analysis of hemagglutinin-neuraminidase glycoprotein gene in human parainfluenza virus type 1 isolates from patients with acute respiratory infection between 2002 and 2009 in Yamagata prefecture, Japa" docx
Ngày tải lên :
18/06/2014, 18:20
... distribution, and reproduction in any medium, provided the original work is properly cited Detailed genetic analysis of hemagglutininneuraminidase glycoprotein gene in human parainfluenza virus type isolates ... number of detected strains in each cluster on the phylogenetic tree was as follows: cluster 1, 84 strains including strains isolated during 2004–2009; and cluster 2, 95 strains from 2003 to 2005, ... these findings in HPIV1 The distribution of amino acids affects the structure of the HN coding region in HPIV1, and previous reports show that substitutions of amino acids in HN glycoprotein reveal...
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Báo cáo hóa học: " Trichloroethylene exposure and somatic mutations of the VHL gene in patients with Renal Cell Carcinoma" potx
Ngày tải lên :
20/06/2014, 00:20
... independently by reamplifying the corresponding fragment and repeating the sequencing procedure using both forward and reverse primers in the samples fixed in Bouin's solution Regarding the codon ... 15 ppm in screw cutting industry during c.332 G>A 58 years women no c.463+1 G>C 42 years man no Other occupational exposures identified cutting oils asbestos cutting oils in screw cutting industry ... without mutation detected in this gene, suggesting that other genes may be implicated in RCC and in particular linked to chemical exposure [24] The VHL gene can be inactivated somatically, besides...
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báo cáo hóa học: " Lung responses to secondary endotoxin challenge in rats exposed to pig barn air" potx
Ngày tải lên :
20/06/2014, 00:20
... gases inside swine confinement buildings Am Ind Hyg Assoc J 1985, 46:658-661 Cormier Y, Israel-Assayag E, Racine G, Duchaine C: Farming practices and the respiratory health risks of swine confinement ... activation including lung vascular inflammation and exacerbate lung inflammation [16,17] In contrast to this, LPS in the barn air is associated with other contaminants in the barn and primarily induces ... challenge Endotoxin or LPS in the barn or intravenously administered LPS alone used in this study will likely induce different host responses Intravenously administered LPS very likely induce systemic...
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Báo cáo hóa học: " Ex vivo promoter analysis of antiviral heat shock cognate 70B gene in Anopheles gambiae" pptx
Ngày tải lên :
20/06/2014, 01:20
... element binding protein (CREB) participates in the heat-inducible expression of human hsp90β gene Chinese Science Bulletin 2001, 46:1645-1649 Perkins ND: Integrating cell-signalling pathways ... binding sites In Silico Biol 2002, 2:S1-15 Myles KM, Pierro DJ, Olson KE: Deletions in the putative cell receptor-binding domain of Sindbis virus strain MRE16 E2 glycoprotein reduce midgut infectivity ... proteins [12-14] Members of the HSP70 family contain three conserved domains: an ATPase domain at the N-terminus, a peptide binding domain, and a GP-rich region at the C-terminus that contains...
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báo cáo hóa học:" In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA" doc
Ngày tải lên :
20/06/2014, 04:20
... MW, Glant TT: VDIPEN, a metalloproteinase-generated neoepitope, is induced and immunolocalized in articular cartilage during inflammatory arthritis J Clin Invest 1995, 95:2178-2186 Nagase H: ... remove traces of FCS After removing HBSS, the cells were trypsinized with ml of trypsinEDTA (BioWhittaker™ Cambrex Bio Science) After examining the cells using an inverted microscope to ensure ... was incubated at 42°C for 90 min, and terminated by heat inactivation at 70°C for 15 Measurement of GAG levels The level of GAG appearing in the medium of explants, cell cultures, and papain-digested...
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Báo cáo hóa học: " Glycerol conversion to 1, 3-Propanediol is enhanced by the expression of a heterologous alcohol dehydrogenase gene in Lactobacillus reuteri" ppt
Ngày tải lên :
20/06/2014, 23:20
... recombinants were cultured in media containing appropriate antibiotics, ampicillin (100 μg/mL) and erythromycin (200 μg/ mL for E coli and μg/mL for L reuteri) Growth was monitored by measuring ... 72°C (20 s) and a final extension at 72°C (1 min) The amplicon was cloned into TA vector to generate the recombinant plasmid pHR1 Further, the yqhD gene was sub-cloned from pHR1 into NcoI/XhoI site ... protein utilizing the principle of protein-dye binding Anal Biochem 72:248–254 doi:10.1016/0003-2697(76)90527-3 Celińska E (2010) Debottlenecking the 1, 3-propanediol pathway by metabolic engineering...
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the epidemiology ofnosocomialinfection due to bacteria carrying ndm-1 gene in vietduc hospital-hanoi, 2010-2011
Ngày tải lên :
25/07/2014, 11:29
... carrying NDM-1 gene 3.1 General information’s of carbapenem resistant bacteria carrying NDM-1 gene Seven species of carbapenem resistant bacteria carrying NDM-1 gene were found, including Enterobacter ... were infected with bacteria strains resistant to carbapenem carrying NDM-1 gene Two patients (2/35; 5.7%) in urology department were infected with strains resistant to carbapenem carrying NDM-1 gene ... carrying NDM-1 gene in environment including A baumannii (1), E aerogenes (2) and was Acinetobacter spp These strains were resistant to antibiotic at high level, but still susceptible to colistin...
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