adipose stem cells tissue engineering and solid organ transplantation and regeneration

... fibroblasts, stem cells, and genetically modified cells have all been explored for their potential as viable cell sources for cartilage tissue engineering (Chung et al., 2008) Human embryonic stem cells ... relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) (Lee ... phenotypic stability of differentiated cells and tissues, delivery systems and biomaterials for cartilage repair, and survivability and integration of the tissue graft to the host cartilage This...

Ngày tải lên: 11/09/2015, 09:57

... Therefore, the exogenous stem/ progenitor cells, such as embryonic stem cells (ESCs), bone marrowor fat-derived mesenchymal stem cells (MSCs), and recently amniotic fluid stem/ progenitor cells, could be ... tracheal bioengineering and transplantation Endogenous and exogenous stem and progenitor cells for lung repair The airway epithelium is subjected to a lifetime exposure by inhaled particles and pathogens ... fluid stem cells can integrate and differentiate into epithelial lung lineages Stem Cells 2008, 26:2902-2911 Takahashi K, Yamanaka S: Induction of pluripotent stem cells from mouse embryonic and...

Ngày tải lên: 10/08/2014, 05:21

... MSC, because adipose stem or stromal cells (ASC) can be readily isolated in considerably larger amounts.6 As mesenchymal stem cells, BMSC and ASC share many biological characteristics, and have both ... functional exhaustion and cell cycle arrest of hematopoietic progenitor cells; and that 7-week CS exposure led to marked depletion of phenotypic bone marrow stem and progenitor cells (HSPC) Moreover, ... vitro and can differentiate into bone, cartilage and fat cells. 4 Stromal cells that fulfill these criteria have been isolated from almost every type of connective tissue, including bone marrow, adipose...

Ngày tải lên: 24/08/2014, 11:56

... expand and differentiate [6] Their origin could be in embryonic cells or in progenitors unrelated to embryonic stem cells Adult stem cells are undifferentiated cells occurring in tissues and organs ... differentiation and self-renewal abilities These stem cells occur in fetal tissues and organs such as blood, liver, and lung and have similar characteristics to their counterparts in adult tissues, ... induced pluripotent stem cells are at least, if not more capable than those from adult (stem) cells Mesenchymal stem cells Although adult stem cells are primarily unipotent cells, under certain...

Ngày tải lên: 18/06/2014, 16:20

... and pluripotential stem cells (hematopoietic tissues) Endothelial cells make up the wall of capillary microvessels, and thus are present in all perfused tissues 2.5 Dynamics In most tissues and ... surrogate systems, artificial organs, and extracorporeal devices), and metabolic process control (receptor–ligand binding, signal transduction, and trafficking) Since an understanding of the cell /tissue ... Polymers as Biomaterials for Tissue Engineering and Controlled Drug Delivery L S Nair · C T Laurencin Interface Tissue Engineering and the Formulation of Multiple -Tissue Systems H H Lu · J Jiang Cell...

Ngày tải lên: 27/06/2014, 10:20

... HO-1MSCs transplantation compared with Null-MSCs and PBS transplantation; and the capillary density was also significantly enhanced by Null-MSCs transplantation compared with by PBS transplantation ... starting two days before and continuing until days after the HO-1-MSCs transplantation Some rats were killed at days after transplantation, and the treated hearts were harvested and cryopreserved in ... homologous recombination and propagated in 293 cells At stipulated time, the supernatant from 293 cells was collected and purified on cesium chloride (CsCl) gradient centrifugation and stored in 10 mmol/L...

Ngày tải lên: 10/08/2014, 05:21

... neural stem cells Science 2000, 287:1433-1438 Prockop D: Marrow stromal cells as stem cells for nonhematopoietic tissues Science 1997, 276:71-74 Smith AG: Embryo-derived stem cells: of mice and ... marrow stem cells (hBMSC), marrow-isolated adult multilineage inducible (MIAMI) cells, and pre-MSCs), umbilical cord blood (unrestricted somatic stem cells (USSCs)), placenta, muscle, and other tissues ... [1,2] Other adult stem cells include neural stem cells (NSCs) [3], and the mesenchymal stem cells (MSCs) that give rise to osteoblasts, chondrocytes, adipocytes, and skeletal and smooth muscle myocytes...

Ngày tải lên: 14/08/2014, 08:20

... other than organ replacement In addition, cancer and traumatic injuries may necessitate the removal of tissues and organs, which again require replacements Traditionally, tissue and organ replacements ... perform many important cell-based functions of tissues and organs, such as self-renewal and repair, hormone production, and metabolism Therefore, tissues and organs that perform important non-mechanical ... 62 Chapter HIF-1 and the Potential Roles of PHIs in Bone Tissue Engineering and Regeneration 64 4.1 Introduction 65 4.2 Bone development and regeneration 65 4.2.1...

Ngày tải lên: 09/09/2015, 11:11

... This strand is referred to as the guide strand The complementary strand, known as the passenger strand or miRNA*, is degraded in most cases and present in lower levels than the guide strand (Schwarz ... the term “neural stem cell”, and the numerous lineage mapping and genetic studies in recent years have given rise to a number of varied ways of characterizing and classifying NSCs and neural progenitors ... Pluripotent stem cell-derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage embryos, offer a renewable and unlimited...

Ngày tải lên: 10/09/2015, 09:08

... Martinez s help, patience and guidance with the animal studies Special thanks to my husband and collaborator, Dr Andrew Krishna Ekaputra, who kept me smiling when times were tough, and generously shared ... 3.6.2 Results and Discussion 68 3.6.3 Conclusion 71 Chapter Peri-implantational fibrosis: a bottleneck in tissue engineering 72 4.1 Biomaterials in tissue engineering ... CZC and Raghunath M Focus on collagen: in vitro systems to study fibrogenesis and antifibrosis —state of the art Fibrogenesis Tissue Repair 2009, 2(1):7 11 2.1 Fibrosis—ubiquitous problem and...

Ngày tải lên: 10/09/2015, 15:52

... Embryonic stem cells 1.2 Gastrulation– formation of mesoderm and endoderm in the embryo .8 1.3 Regenerative medicine and embryonic stem cells 10 1.3.1 Diabetes– a candidate ... from the NIH Stem Cells Information Resource at http://stemcells.nih.gov/info/scireport/chapter7.asp 13 Kawai et al (2008) showed that the patient’s immune system accepted solid organ transplants ... but for the support system my family has been Thank you, Appa and Mummy for all the love and laughter and for inspiring me to follow my heart Sumitha, Aji and Tamanna, and Susan, thank you for...

Ngày tải lên: 04/10/2015, 16:03

... respect to solid organ transplantation exceeds 15,000, yet the number of resulting US Food and Drug Adminis­ tration (FDA) approved biomarker-based diagnostic tests in transplantation stands at ... transcriptomic and proteomic biomarkers of acute and chronic allograft injury and graft accommodation We point out the advantages and pitfalls of trying to identify non-invasive blood-based biomarkers and ... microdissection) and profile them, such tech­ niques are expensive, time consuming and limited by difficulties in obtaining sufficient purified tissue with adequate RNA, and they may affect cell physiology and...

Ngày tải lên: 11/08/2014, 12:21

... engineering and regeneration [134] Mesenchymal stem cells versus embryonic stem cells Embryonic stem (ES) cells are derived from the inner cell mass of the embryonic blastocyst These cells can ... remaining stem cells for tissue regeneration and repair? In conclusion, MSCs derived from adult tissue present an exciting progenitor cell source for applications of tissue engineering and regenerative ... survival and differentiation of stem cells These complementary approaches have been used to compare different groups of stem cells in order to identify core stem genes and to examine supportive tissue...

Ngày tải lên: 09/08/2014, 01:21

... extracted and separated by gravity The resulting 100 cm3 of adipose tissue was then centrifuged at 3500 rpm for five minutes The end result was approximately 40 cm3 of packed adipose tissue, fibrous tissue, ... Cite this article as: Pak: Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose- tissue- derived stem cells: a case series Journal ... the staff of Miplant Stems Clinic Authors’ contributions JP was in charge of patient treatment and follow-up, was responsible for manuscript drafting and revision, and read and approved the final...

Ngày tải lên: 10/08/2014, 23:21

... Teoh Cellular interactions of endothelial progenitor cells and mesenchymal stem cells for vascularised bone tissue engineering Tissue Engineering and Regenerative Medicine International Society (TERMIS-AP), ... Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem Cells ... the tissue engineering strategy (Figure 1-1) Cells and Biomolecules Scaffolds TISSUE Bioreactors Imaging Tools Figure 1-1: Four key technologies used in tissue engineering include cells and their...

Ngày tải lên: 09/09/2015, 10:16

... transitions: twist in development and metastasis Cell 118, 277–279 31 Zipori D (2004) Mesenchymal stem cells: harnessing cell plasticity to tissue and organ repair Blood Cells Mol Dis 33, 211–215 32 ... study of embryonic stem (ES) cell transplantation, we have identified a growth factor combination [HGF and fibroblast growth factors and (FGF1 and FGF4)] to induce mouse ES cells to develop into ... hepatic cells, the findings presented here suggest that MET might be a pivotal factor in determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stem cells for cell transplantation...

Ngày tải lên: 30/03/2014, 04:20

... Currently, several candidates of stem cells, including embryonic stem cell, neuron stem cells, bone marrow-derived mesenchymal stem cell, and peripheral blood-derived stem cells have been frequently ... tissue [3] Interestingly, while stem cell therapy, including bone marrow-derived mesenchymal stem cells [15-17], embryonic stem cells [14] and endothelial progenitor cells [18], have been extensively ... proliferation, and differentiation of stem cells [18,25] Recently, SDF-1α and its receptor CXCR4 are proven crucial in bone marrow retention of hematopoietic stem cells, angiogenesis, and recruitment...

Ngày tải lên: 18/06/2014, 16:20

... integration of the transplanted cells and the host tissue As compared with embryonic stems cells and bone marrow-derived mesenchymal stem cells, adiposederived mesenchymal stem cells (ADMSCs) have the ... collection: in vivo therapeutic potential of human adipose tissue mesenchymal stem cells after transplantation into mice with liver injury Stem Cells 2008, 26:2705-2712 Behling A, Rohde LE, Colombo ... J, Ratajczak MZ: Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis Stem Cells 2005, 23:879-894 Kee HJ, Sohn IS,...

Ngày tải lên: 18/06/2014, 16:20

... (non-hematopoietic stem cells) is still underway The nature of somatic stem/ progenitor cells is much different from that of embryonic stem (ES) cells and induced pluripotent stem (iPS) cells Somatic stem/ progenitor ... of cells has been proposed for the use in tissue engineering, including pluripotent embryonic stem cells (ESC) with all their ethical controversies, adult stem cells found in most tissues, and ... safe, and widespread use of tissue engineering Storage technologies for cells and tissue- engineered products are required for their timely and efficient distribution Furthermore, storage of stem cells...

Ngày tải lên: 28/06/2014, 05:20

... 1155-1160 27 Ogawa R The importance of adipose- derived stem cells and vascularized tissue regeneration in the field of tissue transplantation Curr Stem Cell Res Ther 2006, 1, 13-20 28 Olby NJ, De Risio ... canine adipose tissue- derived mesenchymal stem cells Stem Cells Dev 2008, 17, 681-693 Kang SK, Lee DH, Bae YC, Kim HK, Baik SY, Jung JS Improvement of neurological deficits by intracerebral transplantation ... human adipose tissue- derived stromal cells after cerebral ischemia in rats Exp Neurol 2003, 183, 355-366 Kang SK, Shin MJ, Jung JS, Kim YG, Kim CH Autologous adipose tissue- derived stromal cells...

Ngày tải lên: 07/08/2014, 23:22