adhesion molecules in asthma and airway diseases

... combination of haematoxylin-eosin, Masson trichrome, PAS, alcian blue-PAS and orcein, and evaluated microvessels in the supepithelial lamina propria, showing an increase in vessel number and ... remodelling and respective functional effects in asthma and COPD Structural changes Functional effects Asthma COPD Vascular remodelling with inner airway wall thickening Decreased baseline airway ... increased in asthma and correlated to the vascular permeability of airways [28-30], as well as shown to determine gaps in the Table Factors involved in the bronchial vasculature remodelling in...

Ngày tải lên: 12/08/2014, 11:22

... BALT T cells and B cells for the expression of L-selectin, which binds to PNAd; α4 integrin, which is a subunit of α4β1 integrin (which binds mainly to VCAM-1) and α4β7 integrin (which binds to MAdCAM-1); ... minutes (min), rinsed in PBS, and incubated with peroxidase-streptavidin for 30 After a final PBS wash, slides were incubated with Vector AEC substrate (per manufacturer's instructions), stained ... cells in adhesion molecule expression Lselectin, which binds to PNAd, was expressed on 20% of T cells and almost all B cells in BALT α4 integrin, which is a subunit of α4β1 integrin that binds...

Ngày tải lên: 12/08/2014, 14:20

... collected in the morning, using Vacutainer® tubes (Becton Dickinson Vacutainer Systems, Meylan, France), one month before estimated calving, at calving (within 24 hours after calving) and one month ... immunostaining of CD18 and CD62L adhesion molecules, and for total and differential white blood cell counts Leukocyte counts Total and differential leukocyte counts were determined within h using ... significant increase in the numbers of WBC at calving This was mainly due to an increase in the numbers of circulating neutrophils, and to a less extent, an increase in monocytes At calving, the...

Ngày tải lên: 12/08/2014, 15:20

... populations both from Britain and Japan [5] Allergic diseases such as atopy and asthma are increasingly common in Singapore, and the estimated number of affected individuals stand at around 140,000, ... enough as an indication of clinical expressions of allergy and asthma [15] 2.2 Dynamics of Th-1 and Th-2 in asthma and allergy The Th-1/Th-2 paradigm has dominated our understanding of the pathophysiology ... region of interest turned up the two cytokines IL-12 and IL-13, and therefore the basis of our study stems from delineating the roles that these two cytokines had in atopy in our Singapore Chinese...

Ngày tải lên: 30/09/2015, 14:23

... biological factors The induction of stress-induced intracellular signals, catabolic cytokines, including interleukin-1 (IL-1) and tumor necrosis factoralpha (TNF-α), chemokines, and other inflammatory ... protein kinases are involved in these responses, including protein kinase Cζ, which is upregulated in OA cartilage and is required for activation of NF-κB by IL-1 and TNF-α [113] However, it remains ... chronic inflammation exists, as in RA Conclusions Laboratory investigations in vitro and in vivo regarding the role of the chondrocyte in remodeling the cartilage matrix in the RA and OA joint have...

Ngày tải lên: 09/08/2014, 01:22

... with airway hyperresponsiveness To test this hypothesis, we measured Rrs in nonasthmatic and asthmatic adults during tidal breathing and at maximal inspiration at baseline, following albuterol-induced ... analysis and interpretation of data, and drafting and revising the manuscript SNS contributed to study design, acquisition of data, analysis and interpretation of data, and drafting and revising the ... analysis and interpretation of data, and drafting and revising the manuscript KRL contributed to study design, acquisition of data, analysis and interpretation of data, and drafting and revising the...

Ngày tải lên: 12/08/2014, 13:22

... (cytokines, chemokines, and others) Adhesion molecules expressed and used Contribution of biomechanical events Stimuli and disease processes affecting alveoli compared with airways these changes in ... modulated in part through signaling by ligated adhesion molecules Neutrophil adhesion to endothelial cells induces changes in both neutrophils and endothelial cells that result in cytoskeletal and ... During the acute inflammatory response within regions of the distal lung tissue, neutrophils sequester within the capillary bed Studies examining the role of known adhesion molecules, including...

Ngày tải lên: 12/08/2014, 18:20

... polymorphisms in candidate genes for asthma/ atopy have been identified Linkage and candidate gene studies in asthma and atopy Linkage studies Results from linkage studies (genome-wide searches and candidate ... of inconsistent findings, it is intriguing that chromosomal regions linked to asthma have also shown evidence for linkage to other inflammatory and autoimmune diseases (such as psoriasis, inflammatory ... other genes involved in the allergic inflammation (e.g IL-4Rα [21], β2AR [24]) We can therefore speculate that inconsistent findings in the genetic studies of asthma and atopy summarized in this...

Ngày tải lên: 12/08/2014, 18:20

... decreasing or increasing intra-individual In patients undergoing PTCA, sICAM-1 levels were significantly elevated in comparison with controls and patients with tPA or SK lysis, whereas no intra-individual ... remained significant during the whole observation period No increasing or decreasing intra-individual trends could be observed, either in the tPA group or in the PTCA group SK patients, in contrast, ... Pharmacokinetics and fibrin specificity of alteplase during accelerated infusions in acute myocardial infarction J Am Coll Cardiol 1992, 19:1071–1075 reports Our findings are therefore in accordance...

Ngày tải lên: 12/08/2014, 18:21

... fragmentation and a decreased amount of elastic fibres have been observed in the superficial elastin network of central airways in fatal asthma [12] The presence and extent of airway remodelling in asthma ... real-time imaging tool to estimate the type and degree of airway remodelling in chronic airway diseases such as asthma in the near future Since FCFM has the ability to visualize the airway wall ... patients and controls These findings indicate that FCFM can be used to capture structural changes in the airway wall in humans in vivo, and might become a Table Elastic fibre pattern grading: Paired...

Ngày tải lên: 12/08/2014, 13:22

... the prodomain maintaining the catalytic site inactive and the metalloproteinase domain containing the Zinc binding site ADAM activation mechanisms are mostly similar to MMP's activation and generally ... http://respiratory-research.com/content/10/1/127 Figure Intervention of ADAM/ADAMTS proteinases in asthma and COPD Intervention of ADAM/ADAMTS proteinases in asthma and COPD Succinctly, in asthma, inhaled allergens provoke ... cell-matrix interactions via the interaction with integrins; a cystein-rich domain implicated in cell adhesion; an epidermal growth factor (EGF)-like domain and a cytoplasmic tail involved in various intracellular...

Ngày tải lên: 12/08/2014, 14:20

... guidelines for managing asthma tend to concentrate on treating airway inflammation (18) Acute inflammation in asthma includes both an early and a late phase In the early phase, mast cells and macrophages ... reduced in diseases featuring airway inflammation, such as asthma, increasing DHA levels through supplementation should increase the availability of protectin D1 to alleviate airway inflammation and ... release Calcium binds to calmodulin which activates myosin light chain kinase to phosphorylate myosin, the thick filament in muscle Phosphorylated myosin binds actin, the thin filament in muscle, to...

Ngày tải lên: 24/08/2014, 12:48

... precipitation of insulin B C Fig aB-crystallin protects against the DTT-induced aggregation of insulin, and this activity is enhanced by Arg-HCl Insulin ( , 0.25 mgÆmL)1) was incubated at 37 °C in 50 mM ... of aB-crystallin both aA- and aB-crystallin possess at least two binding modes, and that these are in uenced by both external factors (e.g changes in temperature and pH) and intrinsic factors ... agents in the treatment of protein-misfolding diseases Experimental procedures Materials Intrinsic and extrinsic fluorescence Intrinsic tryptophan fluorescence spectra of aB-crystallin (0.1 mgÆmL)1 in...

Ngày tải lên: 18/02/2014, 16:20

... Tax increases Smoke-free indoor workplaces and public places Health information and warnings Bans on tobacco advertising, promotion and sponsorship Scaling up action against noncommunicable diseases: ... average of US $25 in low-income countries, US$ 50 in lower middle-income countries and US$ 139 in upper middle-income countries By contrast, findings from the second study by the WHO indicate that ... per capita investment of under US$ in low-income countries, US$ 1.50 in lower middle-income and US$ in upper middleincome countries When considered in terms of overall health spending, these...

Ngày tải lên: 20/02/2014, 19:20

... missing links in insulin signal transduction? FEBS Lett 410, 3–10 Dedhar, S., Benjamin, W & Gregory, H (1999) Integrin-linked kinase (ILK): a regulator of integrin and growth-factor signalling ... that of PKB protein and activity in the insulin treated and S-PKB cells, indicating that SLex might be regulated by other insulin-induced factor(s) in addition to PKB signalling pathways It is ... migration and invasion (A) Inhibition by mAbs of the adhesion to HUVECs of untreated control and insulin treated cells (B) Inhibition by monoclonal antibodies of the migration and invasion of insulin...

Ngày tải lên: 21/02/2014, 00:20

... sources included in the model were: road traffic, shipping, aviation, railroad, industries and larger energy and heat producers, small scale heating, working machines, working vehicles, and working ... especially in relation to the prevalence of COPD/chronic bronchitis Results discussion Although asthma and COPD have many risk factors in common and often coexist in clinical settings, and there ... rate in the more polluted city of Malmö (73% vs 80% in the remaining region) Outcome measures The following outcomes were investigated, as obtained by the postal questionnaires: • Diagnosis of asthma...

Ngày tải lên: 06/03/2014, 19:20

... containing CLP and CLAP with a phosphoprotein stain did not reveal phosphate additions to these proteins Only lanes in the gel containing the known phosphoproteins ovalbumin, b-casein and pepsin ... domains or putative GPI unit, if one exists in CLAP as it does in Drosophila fasciclin I and many other fasciclin-containing proteins [36,46] Fas I domains in proteins are almost always found in ... 271) binding domains, so the presence of a GTP/ATP binding region in CLAP may indicate an energy dependent mechanism for pro-CLAP processing or cathepsin L docking and stabilization not found in...

Ngày tải lên: 07/03/2014, 16:20

... selected for its ability to maintain the balance of intestinal microflora It is said to neutralize toxins, bind to pathogenic bacteria, and reinforce intestinal wall integrity which allows animals ... prevent intestinal worm and parasite infestations Regular addition of garlic into the drinking water is said to control intestinal worms Garlic is said to be especially effective when used in conjunction ... Mills Inc are produced by fermenting selected liquid and cereal grain raw ingredients with bakers yeast and drying the entire culture-media without destroying the yeast factors, B-vitamins and...

Ngày tải lên: 08/03/2014, 09:20

... the protein chain According to this model, epitopes in the LDL receptor binding domain are located on one side of LDL, whereas epitopes located in the N-terminal and Cterminal domains are in close ... lipoprotein structure, the assembly and kinetics of apoB containing lipoproteins, and the role of Lp(a) in kidney patients Section reviews clinical chemical methods for diagnosing patients at increased ... Insights into the Assembly and Metabolism of ApoB-Containing Lipoproteins from in vivo Kinetic Studies: Results on Healthy Subjects and Patients with Chronic Kidney Disease 21 Benjamin Dieplinger and...

Ngày tải lên: 17/03/2014, 21:20

... following toxin-mediated damage in vivo Percentage depurination was determined by quantifying the intensity of the liberated fragment in relation to the remaining intact 25S rRNA plus fragment using ... showing key amino acids The active site molecules Y80, Y123 and E177 are shown in green and the position of the two mutated amino acids, F108 and L151, are shown in blue between the aromatic rings ... strains of yeast, in a manner akin to its expression in plant cells [18] Survivors of toxin expression may contain defects in genes normally essential for toxin retro-translocation, refolding,...

Ngày tải lên: 23/03/2014, 07:20