adhesion mechanisms in cancer metastasis

... suppresses actin branching In addition, α-catenin facilitates the anchorage of the N-cadherin-catenin complex to the actin cytoskeleton via actin-binding proteins such as cortactin and α-actinin, thereby ... efficacy in the Page 11 of 16 pre-clinical setting, by inhibiting cancer metastasis, enhancing cancer cell sensitivity to chemotherapeutic agents and delaying castration resistance in prostate cancer ... of the N-cadherin-catenin complex to the actin cytoskeleton via actin-binding proteins, thereby stabilising cell-cell contacts Initial ligation of N-cadherin extracellular domains also triggers

Ngày tải lên: 03/07/2020, 00:57

... Bone Engineering Platform to an In Vitro and In Vivo Breast Cancer Metastasis Model Verena Maria Charlotte Reichert, MD Faculty of Built Environment and Engineering, School of Engineering Systems, ... remodeling and integrin signaling Curr Opin Cell Biol, 2006 18(5): p 463-71 Berrier, A.L and K.M Yamada, Cell-matrix adhesion J Cell Physiol, 2007 213(3): p 565-73 Felding-Habermann, B., Integrin adhesion ... bone metastasis 23 2.8 Osteomimicry of breast cancer cells 26 2.9 Osteolytic bone metastasis 27 2.10 Models investigating bone -cancer cell interaction in vitro 29 2.11 Models investigating

Ngày tải lên: 07/08/2017, 15:52

... women hospitalized for breast cancer surgery were administered questionnaires assessing coping with cancer, defense mechanisms, and memories of parental bonding in childhood Results: High levels ... parental relations (including attachment patterns) can influence interactions with healthcare professionals in breast cancer patients [24–29], thus suggesting that they may modulate more in general adjustment ... et al Coping, quality of life, depression, and hopelessness in cancer patients in a curative and palliative, end-of-life care setting Cancer Nurs 2011;34:302–314 49 Pinquart M, Duberstein PR Depression

Ngày tải lên: 10/01/2020, 13:42

... fixed and stained, and 3D images obtained using regular confocal imaging without sectioning, allowing cell-cell and cell-matrix interactions to be visualized in intact tissue Ki67 staining for cell ... placed in a humidified incubator at 37 °C (Day 0) For live assays, eggs were windowed at 2– days by dropping the level of the albumen using a syringe and needle then making a small window in the ... invasion, intravasation, extravasation, seeding and colonization at distant sites [1, 2] Both in vitro and in vivo experimental models have been used to gain further insight into metastatic mechanisms

Ngày tải lên: 24/07/2020, 01:23

... activity of β2 integrin, we ruled out the participation in the adhesion observed in β2-C26 since neutralizing antibodies against β1 integrins could to abrogate C26 cell adhesion nor induce a higher ... tumor-induced T regulatory cells and T-cell anergy in tumor-bearing host Cancer Res 2006;66(2):1123–31 Lin WW, Karin MA Cytokine-mediated link between innate immunity, inflammation and cancer J Clin ... BMC Cancer (2017) 17:827 Page of 17 Cancer cell lines Cancer cell adhesion assay All in vitro and in vivo experiments were conducted using the murine C26 colon adenocarcinoma (C26) cell line

Ngày tải lên: 06/08/2020, 03:31

... (iROS) in BC cell lines in a time-dependent manner Sal has been shown to induce iROS production, suppress the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathways ... A, Ying S, Schmidt T, Heger U, Schneider M, Ulrich A Salinomycin inhibits metastatic colorectal cancer growth and interferes with Wnt/beta-catenin signaling in CD133(+) human colorectal cancer ... Kim S, Ro J, Kang HS, Yoon S Salinomycin, a pglycoprotein inhibitor, sensitizes radiation-treated cancer cells by increasing DNA damage and inducing G2 arrest Investig New Drugs 2012;30(4):1311–

Ngày tải lên: 06/08/2020, 05:36

... clones in an established ovarian cancer cell line HEY using shRNA-mediated silencing The expression of integrins in cell lines was evaluated by flow cytometry Spheroid forming ability, adhesion ... surrounding microenvironment and play a fundamental role in promoting metastasis This study investigates the molecular mechanism which associates CSCs and integrins in ovarian cancer metastasis ... remodeling requisite for tumor metastasis in ovarian cancer patients Keywords: Ovarian carcinoma, Cancer stem cells, Metastasis, Integrins, Chemoresistance, Recurrence, Oct4A Background Ovarian cancer

Ngày tải lên: 21/09/2020, 01:47

... pro-inflammatory cytokines, indicating that fluoxetine treatment may promote the entry of cancer cells into the brain via changes in the function of the BBB This provides important insight into ... with fluoxetine for three weeks prior to the intracardiac injection of 231BR breast cancer cells Administration of fluoxetine in drinking water resulted in therapeutic concentrations in the serum ... cytokine production This in turn may affect BBB permeability and lead to increased brain metastasis of circulating breast cancer cells Fluoxetine enhances glial activation in the vicinity of brain

Ngày tải lên: 14/10/2020, 13:56

... methylated in certain breast cancer cell lines, including BT-474 and MDAMB-231, as well as in MCF10A cells, indicating this methylation is not cancer specific (Figure 1B) Bisulfite genomic sequencing ... activated by inhibition of EZH2 in synergy with histone deacetylase inhibitor Consistent with the overexpression of EZH2 in a variety of human cancers including breast cancer, CDKN1C in these cancers ... Institute of Singapore, A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing, China, Information

Ngày tải lên: 01/11/2022, 09:06

... cytoskeleton, the cells had been fixed and stained for F-actin (visualized by means of rhodamine-phalloidin staining) and 4′​,6-diamidino-2-phenylindole (DAPI) staining after cultivation for 2 h, 4 h, ... as signaling pathways18 In this study, we used the method of annulling gravity by a Random Positioning Machine (RPM) to find alterations of the MCF-7 breast cancer cell growth behavior in concert ... aggregates were further investigated by histochemistry using hematoxylin-eosin (HE) and Periodic Acid-Schiff (PAS) staining Figure 2A shows normal HE-stained MCF-7 breast cancer cells Figure 2B–D

Ngày tải lên: 04/12/2022, 14:48

... ATP binding site serves as a docking site for specific binding of cytoplasmic signaling proteins containing Src homology-2 (SH2) and protein tyrosine binding (PTB) domains. These proteins in turn ... tyrosine kinase inhibitors. Inhibiting the activity of tyrosine kinases by low molecular weight compounds capable of interfering with either ligand binding (in the case of receptor tyrosine kinases) ... emanating from tyrosine kinase endows these enzymes a dominating oncoprotein status, resulting in the malfunctioning of signaling network [3]. The discovery that SRC oncogene having a transforming

Ngày tải lên: 03/11/2012, 09:57

... filtering for narrowing down candidates Studies that rely on this principle of genomic and transcriptomic integration are generating new insights into mechanisms of human diseases including cancer ... we designated the staining index of 6-9 as the ―normal‖ baseline expression of THY1 Accordingly, a staining index of 1-4 was considered as reduced expression, and a staining index of was considered ... staining results for THY1 Immunohistochemical staining of THY1 in NPC TMA containing noncancerous nasopharyngeal mucosa, primary NPC, and lymph node-metastatic NPC A) Representative staining...

Ngày tải lên: 11/09/2015, 09:05

... hinge region, which is between the N-terminal ECM binding domain and the C-terminal LAP binding domain This can be achieved by many proteinases in the serine proteinase family, such as plasmin, ... Tyr583 are involved both in GDP and ATP binding to TG2 The residue Arg580, which is important for GTP/GDP binding, is not involved in ATP binding (Han et al 2010) By purifying the insulin-like growth ... Immunofluorescence IFN-γ Interferon-gamma IGFBP-3 Insulin-like growth factor-binding protein-3 IκBα Inhibitor of kappa B alpha ILK Integrin-linked kinase IP3 Inositol 1,4,5-trisphosphate xviii i.p Introperitoneal...

Ngày tải lên: 24/08/2014, 10:58

... 10% of all cancer incidence and mortality in 20132 Consistent with trends in developed countries, CRC is the most frequent cancer in Singapore, accounting for 14% to 18% of all cancer incidence ... adherent cells following 40 incubation in fibronectin-coated wells (A-B) (*** p

Ngày tải lên: 09/09/2015, 11:25

... Chapter Clinical Blood Processing to Detect and Analyze Circulating Cells 108 6.1 CTCs in Patients with Renal Cell Carcinoma (Kidney Cancer) 108 6.1.1 Blood Sampling of Metastatic Renal Cancer ... it simple to be integrated to existing laboratory microscopes and immunofluorescence staining can be done in situ to distinguish cancer cells from hematopoietic cells This also minimizes the use ... scanning (Pieterman, van Putten et al 2000) As technology develops, it will increasingly have a broader impact in biology and healthcare, bringing more sensitive instruments for various clinical...

Ngày tải lên: 11/09/2015, 09:17

... carboxyl-terminal tail as illustrated in Figure 1.5C appears to be the docking site for at least two interacting sites including calmodulin (CAM) binding sites and region involved in microfilament-associated ... cell adhesion proteins such as E-cadherin and β-catenin, which may account for the decrease in cancer cell plasticity following TRPV4 knockdown Our preliminary data showed that Twist might be involved ... Ecadherin and β-catenin In conclusion, this study shows that TRPV4 plays a novel role in cellular processes associated with metastasis and provides insights into the mode of action of TRPV4 in metastasis...

Ngày tải lên: 02/10/2015, 17:15

... http://nj.gov/health/ces/reports.shtml, including: • Cancer Incidence and Mortality in New Jersey 2000-2004; • Cancer Incidence Rates in New Jersey’s Ten Most Populated Municipalities 1998-2002; • Trends in Cancer Incidence ... pharynx) cancer, ovarian cancer, pancreas cancer, prostate cancer, stomach cancer, thyroid cancer, and urinary bladder cancer To compare incidence rates in the poverty area groups, incidence rate ratios ... of smoking reported among persons living in poverty in the U.S.13 may be one of the main reasons for the increased incidence of oral cancer in the areas with high poverty See Tables 2-9 in Appendix...

Ngày tải lên: 14/02/2014, 21:20

... of invasive tumor cells show uniform, intense membrane staining IHC (Herceptest®) Scoring Breast Cancer Cell Staining pattern Score Interpretation No staining Negative Faint incomplete staining ... an initial step that is followed by validation in cancer cells and eventually cancer patients Increasing the Number of Biomarkers in Clinical Use In Chapter 1, we described the expanding interest ... my cancer responding to this treatment? Will my cancer come back? After Cancer Diagnosis Post Treatment Uses of Biomarkers in Cancer Drug Discovery In addition to their uses in cancer medicine,...

Ngày tải lên: 14/02/2014, 21:20

... Research Multidisciplinary Approaches in Cancer Treatment Investigators are often interested in investigating multidisciplinary approaches in cancer care The advantages of studying the entire approach ... several ways including: • assisting applicants in locating funding opportunities; • directing applicants to grant mechanisms that match the goal or intent of the project and experience of the investigator ... consolidating and focusing cancer- related activities, increasing research productivity, promoting shared use of research resources and improved quality control, stimulating and promoting interdisciplinary...

Ngày tải lên: 14/02/2014, 22:20

... combining intervention groups may have been to increase power, this post hoc combining of heterogeneous groups likely resulted in increased within-subject error, decreasing the likelihood of finding ... reported In medicine, recognition that many randomized clinical trials were not being reported in a manner that allowed independent evaluation led to calls for reform, culminating in the original ... care Cointervention confound The authors distinguish their trial from studies examining psychosocial interventions, stating, “this is the first [trial] to examine the impact of nursing interventions...

Ngày tải lên: 15/02/2014, 05:20