activation of nuclear factor kb

... cells via activation of NF-jB Attenuation of LXF-289 cell proliferation by ATP is mediated by the activation of the MEK ⁄ ERK1 ⁄ 2, PI3K and p38 MAPK pathways Activation and translocation of the ... by activation of the NF-jB1 isoform of transcription factor NF-jB, the MAPKs ERK1 ⁄ and p38 and PI3K Thus, extracellular nucleotides need to be added to the class of the critical regulators of ... factor of proliferation and apoptosis Attenuation of LXF289 cell proliferation by extracellular ATP is mediated by the induction of nuclear translocation of p50 NF-jB1 and possibly new formation of...

Ngày tải lên: 07/03/2014, 12:20

... value of Data were expressed as the mean value ± standard error of the Figure Detection of p38 MAPK activation To detect the activation of p38 MAPK by S100A8/A9, monocytes, at a density of × 106 ... U/pg), proving the activation of p38 MAPK by S100A8/A9 Involvement of NF-κB activation in S100A8/A9 stimulation The transcription factor NF-κB plays a prominent role in the activation of multiple inflammatory ... independence of p38 mitogen-activated protein kinase (MAPK) activaS100A8/A9-induced nuclear factor kappa B (NF-κB) activation and its independence of p38 mitogen-activated protein kinase (MAPK) activation...

Ngày tải lên: 09/08/2014, 07:20

... study of Bcl-xL using siRNA may further confirm their role of taxol-treated DCs Although the expression of Bax was increased in TaxolDCs, the expression of Bax occurred later than that of Bcl-xL, ... performed under the program of the Basic Atomic Energy Research Institute (BAERI), which is a part of the Nuclear R&D Programs funded by the Ministry of Science & Technology (MOST) of Korea References ... enhanced cytokine production of DCs treated by taxol The TaxolDCs treated with μg/ml of taxol produced higher amounts of both cytokines than the ContDCs, at 24, 48 h of incubation time (Fig 2)...

Ngày tải lên: 07/08/2014, 23:22

... changed by any of the four types of CRLPs Effect of the fatty acid composition of CRLPs on COX-2 mRNA expression The effect of CRLPs of varying fatty acid composition on expression of COX-2, an ... effect on the secretion of TGFb at any of the time points assessed (Fig 3E) The abundance of mRNA transcripts for each of the cytokines was determined after incubation of THP-1 macrophages with ... (Fig 4E) Effect of the fatty acid composition of CRLPs on NF-jB activation in macrophages The p65–NF-jB DNA-binding ELISA (TransAMÔ) was used to assess the influence of CRLPs on NF-jB activation THP-1...

Ngày tải lên: 23/03/2014, 04:20

... sites of HIV-1 NF -kB or Igk NF -kB, as indicated, on the interaction between purified NF -kB p50 (A) or p52 (B) and 32P-labelled IgK NF -kB DNA–DNA target molecules A total of 10 ng of NF -kB factors ... molecules A total of 10 ng of NF -kB factors were incubated for 20 in binding buffer in the absence ( –) or in the presence of the indicated concentrations of NF -kB DNA –DNA and NF -kB PDP–PDP molecules, ... and p50 NF -kB transcription factors In addition, Fig 4A shows that, unlike NF -kB PDP –PDP molecules, NF -kB PNA–PNA hybrids not affect the binding of p50 and p52 NF -kB transcription factors to...

Ngày tải lên: 24/03/2014, 04:21

... HIF-1 signaling pathways The FIH interaction with inhibitor of nuclear factor- kappaB alpha (IjBa) or p105 [the precursor of p50 nuclear factor- kappaB (NF-jB)] was discovered before its interactions ... HIF-1a and NF-jB are key factors of the hypoxic and inflammatory responses, respectively, the possibility of crosstalk between two factors is of growing interest Many lines of evidence support the ... macrophages of IkB kinase-b knockout mice, that NF-jB plays a critical role in transactivation of the HIF1A gene and that basal activity of NF-jB is required for hypoxic induction of HIF-1a In...

Ngày tải lên: 29/03/2014, 23:20

... options List of Abbreviations used CGRP: calcitonin gene-related peptide CGRP; OPG: osteoprotegerin; RANK: receptor activator of nuclear factor- B; RANKL: receptor activator of nuclear factor B ligand; ... studies of the interaction of osteoclasts, osteoblasts, neurotransmitters, and the OPG/RANKL/RANK system have to be undertaken to gain a better understanding of the multifactorial process of aseptic ... reference for the relative quantification of the gene of interest, which was expressed as the ratio of ‘concentration of the target’ to ‘concentration of b-actin’ Western Blot MG-63 cells were...

Ngày tải lên: 20/06/2014, 04:20

... options List of Abbreviations used CGRP: calcitonin gene-related peptide CGRP; OPG: osteoprotegerin; RANK: receptor activator of nuclear factor- B; RANKL: receptor activator of nuclear factor B ligand; ... studies of the interaction of osteoclasts, osteoblasts, neurotransmitters, and the OPG/RANKL/RANK system have to be undertaken to gain a better understanding of the multifactorial process of aseptic ... reference for the relative quantification of the gene of interest, which was expressed as the ratio of ‘concentration of the target’ to ‘concentration of b-actin’ Western Blot MG-63 cells were...

Ngày tải lên: 20/06/2014, 07:20

... TNF-α-induced activation of JNK, or nuclear transport of Smad2 or the large T antigen This molecule should therefore be considered a unique inhibitor of NF-κB that acts at the level of nuclear translocation ... 10) (a) Sum of the thickness of the right and the left hind paws of each mouse after weeks of treatment Each paw was measured twice and the average was plotted (b) Sum of the number of swollen ... transcription factor nuclear factor- κB (NF-κB) forms a heterodimer or a homodimer of the subunit members, and in the cytoplasm of unstimulated cells it binds to natural inhibitors of NF-κB (IκB),...

Ngày tải lên: 09/08/2014, 07:20

... selected for analysis (Figure 1) The first, of 56 kb, includes the TNFAIP3 gene and the region of high LD with polymorphisms in the gene The second, of 65 kb, is the LD region that includes the two ... interpretation of results JJG-R coordinated the acquisition of clinical data and collection of samples, and participated in the analysis and interpretation of results AG participated in the design of the ... and in the coordination of acquisition of clinical data and collection of samples, and supervised genotyping, statistical analysis, interpretation of results and writing of the manuscript All authors...

Ngày tải lên: 09/08/2014, 14:20

... of level of NF-κB in the nuclear fraction using biotin detection (B) A representative of the EMSA analysis of level of AP-1 in the nuclear fraction by non-autoradiograph Table 2: Percentage of ... smokeFigureon NF-κB the impact of local administration of decoy exposed1mice ODNs Demonstration of the impact of local administration of decoy ODNs on NF-κB activation in the lungs of 92 day smoke-exposed ... The role of nuclear factor κB in cytokine gene regulation Am J Respir Cell Mol Biol 1997, 17:3-9 Ho TY, Bagnell CA: Relaxin induces matrix metalloproteinase9 through activation of nuclear factor...

Ngày tải lên: 12/08/2014, 14:20

... of curcumin inhibit the induction of degradative and inflammatory pathways by IL-1β through revoking activation of caspase-3, production of COX-2, phosphorylation of Iκ-Bα and inhibition of nuclear ... mainly mediated by inhibiting IL-1β-induced activation of NF-κB and thus suppression of caspase-3 activation, of production of COX-2 and upregulation of MMPs [9] In this study we demonstrate that ... down-regulates the constitutive activation of nuclear factor- kappa B and IkappaBalpha kinase in human multiple myeloma cells, leading to suppression of proliferation and induction of apoptosis Blood 2003,...

Ngày tải lên: 12/08/2014, 14:22

... http://arthritis-research.com/content/13/3/R104 Page of Figure Effect of pertussis toxin (PTX) on CXCL10-induced expression of receptor activator of nuclear factor kappa-B ligand (RANKL) Expression was ... pertussis toxin; RA: rheumatoid arthritis; RANK: receptor activator of nuclear factor- kappa B; RANKL: receptor activator of nuclear factor- kappa B ligand; RT-PCR: reverse transcriptasepolymerase chain ... http://arthritis-research.com/content/13/3/R104 Page of osteoclastogenesis of RA synovial tissue and subsequent joint erosion Additional material Additional file 1: Receptor activator of nuclear factor kappa-B ligand (RANKL)...

Ngày tải lên: 12/08/2014, 17:22

... corresponding to kb upstream of the known transcription start site of each transcript was taken In case of genes yielding more than one transcript, the window was enlarged to give a stretch of sequence ... phosphorylation of nuclear factor I in malignant glioma cell lines J Biol Chem 2000, 275:30668-30676 Wang W, Stock RE, Gronostajski RM, Wong YW, Schachner M, Kilpatrick DL: A role for nuclear factor I ... subunit of the GABA-A receptor, is a direct target of NFI-A [14], which is in accordance with the results of our promoter analysis Thus, it is tempting to speculate that the reduced expression of...

Ngày tải lên: 14/08/2014, 07:21

... 1.10 Members of NF-κB family 52 1.11 Canonical and non-canonical pathway of NF-κB 54 1.12 The nuclear regulation of NF-κB 58 1.13 Domain organization of the Rip family 59 1.14 Network of the Rip ... pathogenesis of allergic airway inflammation with persistent nuclear factor (NF)-κB signaling pathway activation This association has made NF-κB, a master pro-inflammatory transcription factor, an ... source of motivation to persevere and strive for excellence in my work III Table of contents Declaration I Acknowledgements II Table of contents IV Summary VIII List of tables X List of figures...

Ngày tải lên: 08/09/2015, 18:35

... activation of epithelial cells is dependent on the expression of PRRs and the expression of signaling proteins downstream of PRRs The greater the number of PRRs, the greater the sensitivity of ... and nuclear translocation of transcription factor signal transduction and activator of transcription (STAT)-6 In the nucleus, STAT-6 results in the expression of GATA-3, a transcription factor of ... the expression of Th2 cytokines, GATA-3 also inhibits the activation of T-Bet, a transcription factor of Th1 cytokines (Ouyang et al., 1998) Notably, the expression of GATA-3 activation is dependent...

Ngày tải lên: 08/09/2015, 18:36

... prevent asthma Therefore, the major unmet needs of this area include better management of the severe forms of the disease and the developments of curative therapies (Akdis, 2012) Consequently, ... anti-inflammatory approach for attenuation of allergic airway inflammation would be through appropriate and specific inhibition of signaling molecules which regulate the activation of NF-κB (Uwe, 2008) Rip-2 ... mediate the formation of functional IKK complex, which is essential for NF-κB activation; while RPS-3 has been demonstrated to enhance the binding of NF-κB 76 to the κB sites of selected NF-κB targets...

Ngày tải lên: 08/09/2015, 18:36

... TBP Cytosolic p65 IκBα * Nuclear p65 * * B Figure 4.7 Effects of S2 on NF-κB activity Immunoblotting of cytosolic extract of lung tissues and p65 NF-κB in nuclear extract of lung tissues isolated ... ring of cells cell layer deep; 3, a ring of cells 2-4 cells deep; 4, a ring of cells of >4 cells deep To determine the extent of mucous secretion, percentage of airway epithelium covered with mucous ... mucous; 1, 75% of airway covered with mucous Scoring of inflammatory...

Ngày tải lên: 08/09/2015, 18:38

... autoubiquitination 42 3.2.6 Effect of PMX290 on Keap1-dependent nuclear shuttling of Nrf2 44 3.2.7 Effect of PMX290 is independent of cysteine 151 in Keap1 3.3 Summary 48 52 4.0 Activation of Nrf2 by Andrographolide ... MAPK Mitogen-activated protein kinases NF-E2 Nuclear factor erythroid-2 NF- κB Nuclear Factor- kappaB Nrf2 Nuclear factor erythroid-2 (NF-E2)-related factor Nqo1 NAD(P)H:quinine oxidoreductase ... 2010) The activation of antioxidant defense mechanisms include the upregulation of the expression of genes involved in the detoxification of xenobiotics and carcinogens, maintainence of redox...

Ngày tải lên: 09/09/2015, 08:16

... the activation of all of these four pathways is necessary for the effective induction of cytokines ⁄ chemokines in HUVECs by AGE-HSA and LPS Notably, blocking p38 with SB203580 led to a more profound ... and nuclear factor- jB (NF-jB) activation Results AGE-human serum albumin (HSA) and LPS stimulate a time- and dose-dependent cytokine ⁄ chemokine production To determine the expression profiles of ... expected, incubation of HUVECs with LPS led to activation of both MAP kinases (Fig 3A) and NF-jB (Fig 3B) Similar to LPS, AGE-HSA stimulation also induced phosphorylation of p38, extracellular...

Ngày tải lên: 30/03/2014, 01:20