a novel class of drug molecules

... 16 kDa) Amblyomma americanum (Zhu et al., 199 7a) • Lack of functional characterization Calcaratin (14 kDa) Unnamed (45 kDa) Tabanin (7 kDa) Boophilus calcaratus (Motoyashiki et al., 2003) Anopheles ... from hematophagous animals 46 Chapter two Table 2.1 Anticoagulation activities of Amblyomma variegatum SGE (females fed for days) 71 Table 2.2 Anticoagulation activities of A variegatum SGE and RPHPLC ... segment of basic residues near the C-terminus (Figure 1.5 A) (Zhang et al., 2002) 1.5.1.8 Madanins and chimadanin Madanins (isoforms and 2) (Iwanaga et al., 2003) and chimadanin (Nakajima et al.,...

Ngày tải lên: 14/09/2015, 14:11

... TTTCCCGTTTATCAAATC-3¢ and 5¢-CCGCTCGAGT CATTACTAGGTTCCCTGTCCAGTGTTACC-3¢, and PDE1(Lys321–Thr620) was amplified with primers 5¢-GGG AATTCCATATGAAGAATGATCAATCTGGCTGCG GCGCAC-3¢ and 5¢-CCGCTCGAGTCATTACTAGG ... truncated fragments of TbPDE1 were also amplified using the same protocol and pET-PDE1 as template PDE1(Arg189–Thr620) was amplified using the primer pairs 5¢-GGGAATTCCATATGAGAGACAATA TTTCCCGTTTATCAAATC-3¢ ... sequence of its catalytic domain is approximately equidistant from those of all mammalian class I PDEs, the class I PDEs dunce of D melanogaster, regA of D discoideum and PDE2 of S cerevisiae, as well...

Ngày tải lên: 19/02/2014, 12:20

... 5'-TATAGTCGACCACCCGGACTCAGAGTCTCCT-3' and 5'-ATATGGATCCATCTCAGTCCCTCACAAGA-3' (HLAB), 5'-ACCTGTACGCCAACACAGTG-3' and 5'-GCCATGCCAATCTCATCTT-3' (β-Actin), 5'-CTGAAGGAGACCATTGGTGA and 5'-GGTACTGGTACACAGTTCGA-3' ... and 5'-GGACGTAGGGTAAACGTCAGC-3' (TAP2), 5'-CTCGCGCTACTCTCTCTT3' and 5'-AAGACCAGTCCTTGCTGA-3' (β2m), 5'-TATAGTCGACCACCCGGACTCAGAATCTCCT-3' and 5'ATATGGATCCATCTCAGTCCCTCACAAGA-3' (HLA -A) , 5'-TATAGTCGACCACCCGGACTCAGAGTCTCCT-3' ... β-actin and CD45, 59°C for TAP1 and 60°C for HLA -A and HLA-B The primer sequences were: 5'-GAGACATCTTGGAACTGGAC-3' and 5'-CTCTGAGTGAGAATCTGAGC-3' (forward and reverse, TAP1), 5'GTACAACACCCGCCATCAG-3'...

Ngày tải lên: 18/06/2014, 15:20

... and, in small amounts, terminal-Rha and 6-substituted-Glc In addition, the disaccharide 7-O-carbamoyl-Hep-(1fi3)Hep was found Fatty acid analysis revealed the presence of typical fatty acids of ... structural characterization NMR spectroscopy and MALDI-TOF MS spectrometry of oligosaccharide Oligosaccharide was isolated by gel permeation chromatography after complete deacylation of the LOS of ... Negative ion MALDI-TOF mass spectra of oligosaccharide obtained in linear mode at normal (A) and higher laser intensity (B) Assignments of main ion peaks are shown P, phosphate; Pyr, pyruvic acid...

Ngày tải lên: 19/02/2014, 13:20

... from Takara Shuzo (Kyoto, Japan), Toyobo (Osaka, Japan), and New England Biolabs (Beverly, MA, USA) All other reagents were of analytical grade from Nacalai Tesque (Kyoto, Japan) and Wako Pure ... each primer (5Â-GGAAT TCCATATGTCCGCACCTTCCACCAGCACCG-3Â and 5Â-GGGAAGCTTTCAGCCAAGCAGCTCTTTCAGG-3Â), 2.5 U LA Taq DNA polymerase, and 115 ng genomic DNA from P putida ATCC12633: preincubation at ... concentrations of NADPH and xed concentrations of pyruvate These indicate a sequential mechanism for the NMAADH reactions In fact, the data obtained gave a good global t to the equation of an ordered...

Ngày tải lên: 19/02/2014, 16:20

... second set, A5 2 (5¢AGAGTGAGCAGGTAGCGAGAGGAG-3¢)/TRHR-8 (5¢-GGGGGTGTAGAGGTTTCTGGAGAC-3¢); third set, A5 3 (5¢-CGAGAGGAGCATTAGA-TAGATG Ó FEBS 2002 CAG-3¢)/TRHR-9 (5¢-GCCGAAATGTTGATGCCCA GATAC-3¢) (Fig ... CGTAACTTTTGCTG-3¢)/TRHR1-4 antisense (5¢-TC TGTTAAATGTACCTAAGTAGGCA-3¢) and TRHR2-2 sense (5¢-CAGCAAAATGGAAAATAGTAGC-3¢)/ TRHR2-4 antisense (5¢-CGACACTGTAGTAG-AGAT CACC-3¢), respectively The PCR ... 32281–32287 31 Itadani, H., Nakamura, T., Itoh, J., Iwaasa, H., Kanatani, A. , Borkowski, J., Ihara, M & Ohta, M (1998) Cloning and characterization of a new subtype of thyrotropin-releasing hormone...

Ngày tải lên: 21/02/2014, 03:20

... with alternative amino acid substrates GDH from mammalian sources is highly regulated by a diverse array of small molecules, with ADP, GTP, leucine and the combination of malate and palmitoyl CoA ... relative quantitation was achieved using the ratio of the height of the emerging peak to that of the undigested GDH For MALDI-TOF calibration purposes, BSA was used as a standard and was diluted ... glutamate is present is largely negated by the small increase in stability caused by glutamate Norvaline, to a much greater effect, stabilizes the protein and again zinc has a minimal effect Although...

Ngày tải lên: 05/03/2014, 23:20

... GACCTCATGACGGCGGACGTGGACCG Down: CGGTCCACGTCCGCCGTCATGAGGTC Ser64 fi Ala Glu168 fi Ala Val169 fi Ala His170 fi Ala Asn189 fi Ala Arg191 fi Ala Asp192 fi Ala Leu193 fi Ala Leu196 fi Ala The concentrations of ... fi Ala Ser64 fi Ala Glu168 fi Ala Val169 fi Ala His170 fi Ala 1.13 2.49 1.53 21.67 1.53 1.19 2.20 1.40 19.17 1.40 1.10 Asn189 fi Ala Ala190 fi Ala Arg191 fi Ala Asn192 fi Ala Leu193 fi Ala Leu196 fi Ala ... Up: CACTCGTCGAGGCCCATACCGAGCAG Down: CTGCTCGGTATGGGCCTCGACGAGTG Up: CGTCGAGGTCGCTACCGAGCAGGAAG Down: CTTCCTGCTCGGTAGCGACCTCGACG Up: GGTGATTGGCGTTGCCGCCCGCGACC Down: GGTCGCGGGCGGCAACGCCAATCACC...

Ngày tải lên: 07/03/2014, 03:20

... oligomer Nucleic Acids Res 32, 1325– 1334 Nakahata S, Kotani T, Mita K, Kawasaki T, Katsu Y, Nagahama Y & Yamashita M (2003) Involvement of Xenopus Pumilio in the translational regulation that is specific ... ERK-MAPK antibody was obtained from New England Biolabs (Ipswich, MA, USA) (9106S) The b-tubulin and HA antibodies were obtained from E7 (Iowa Hybridoma Bank, Iowa City, IA, USA) and 12CA5 (Abcam, ... Watanabe H, Yamashita M, Kashiwabara S, Miyado K & Baba T (2006) Possible role of mouse poly (A) polymerase mGLD-2 during oocyte maturation Dev Biol 289, 115–126 Rouhana L et al (2005) Vertebrate...

Ngày tải lên: 07/03/2014, 05:20

... Thermoplasmata class of the Euryarchaeota and in Nanoarchaeum equitans Also, they are absent from the Bacteria and Eukaryota All of the Archaea that have archaeal-type PS and PANK universally lack ... panC ORF was amplied from genomic DNA of E coli strain XL1Blue (Stratagene) using Pfu polymerase and the primers dCGCGCCTCGAGGAGGAGTCACGTTATGTTAATTA TCGAAACC and dGCGCGTCTAGATTACGCCAGCTC GACCATTTT ... to bacterial or eukaryotic PS and PANK isoforms In fact, bacterial PS is entirely absent from the archaeal domain, and archaeal homologs to bacterial PANK are limited to the Thermoplasmata class...

Ngày tải lên: 07/03/2014, 05:20

... (5¢-GAATTCatgaaggttctcctccactg-3¢) and rO-MACS-XhoI-D (5¢-CTCGAGtgcccgtccccactcctggt-3¢) for o-macs; EcoRI-mMACS1-U (5¢-GAATTCatgcagtggc tgaagagttt-3¢) and mMACS1-XbaI-D (5¢-TCTAGAtagct gaccaaactccttg-3¢) ... Tanaka, T., Hosaka, K & Numa, S (1981) Long-chain acyl-CoA synthetase from rat liver Methods Enzymol 71, 334–341 20 Yoshihara, Y., Kawasaki, M., Tamada, A. , Fujita, H., Hayashi, H., Kagamiyama, ... octanoate; C10, decanoate, C12, dodecanoate, C16, palmitate, BA, benzoate The data are means ± SD of triplicate assays transcripts for the SA and MACS1 genes are present only in the RE area of...

Ngày tải lên: 08/03/2014, 02:20

... 5¢-TTCAATGTGTCTGATTGGGCAGCTCTACTAGA AAAGGCTG-3¢; for E318 to I, 5¢-CAATGTGTCTGA TATAGCAGCTCTACTAGAAAAG-3¢; for E318 to R, 5¢-CAATGTGTCTGATCGAGCAGCTCTACTAGAAA AG-3¢; for E318 to Y, 5¢-CAATGTGTCTGATTATGCA ... study Lancet 353, 351–353 20 Matsubara, Y., Murata, M., Maruyama, T., Handa, M., Yamagata, N., Watanabe, G., Saruta, T & Ikeda, Y (2000) Association between diabetic retinopathy and genetic variations ... ‘open’ and ‘closed’ forms of a2 VWFA domain (A) Alignment of C-terminal sequence of a2 and aM VWFA domains showing a2 E318 and aM F302 (boxed) Secondary structural elements are marked above the alignment;...

Ngày tải lên: 08/03/2014, 22:20

... GCGAAGCTTACCAGACCGTGGACTAACGA CCCACCGTCTTCGAGAACTA CTTCCTTGGTCTTGGCAGAG CCAGACTAGATGTAGTATTTTTTG ATTAGAGCCAGATGCTTAAGTCC ACCACAGTCCATGCCATCAC TCCACCACCCTGTTGCTGTA and then treated with TGFb1; alternatively cells ... antisense hRhoA sense hRhoA antisense GAPDH-F GAPDH-R Sequence (5¢- to 3¢) GGGATCAGAGTTCATAGTGAAAAGAG GCGAAGCTTCGGCCTAGCTCTCTCCCGGGTCTC GCGGGTACCAATGTGATGGGTGGACTGGT GCGAAGCTTACCAGACCGTGGACTAACGA ... change Cells appeared elongated or spindle-shaped, with a parallel arrangement of actin bundles (Fig 5J) Addition of TGFb did not cause any additional changes in actin cytoskeleton organization...

Ngày tải lên: 16/03/2014, 06:20

... mutagenic primers were: GRRF-PDZ1: 5¢GAAAGGGGAAATTCAGGGCGTCGT TTCAGCATTGCAGGAGG-3¢; GRRF-PDZ2: 5¢-ATTA AAGGTCCTAAAGGTCGTCGGTTTAGCATTGCTGGA GG-3¢; RAAV-MEK2: 5¢-CACCCACGCGCGCCGCCGT GTGA-3¢ and ... 5¢-GTGGGGAAATATGCTCTTGAGGAGGT-3¢; primers 2, forward: 5¢-GTGACTTCAGAGACACTGCCA-3¢, and reverse: 5¢-CCCTTTCAAGTGTGATTTCTTC3¢; primers 3, forward: 5¢-ACCAGATGGTGAGAGCGAT-3¢, and reverse: 5¢-CTGTCTTTCATAGGTCCCAAT-3¢ ... RT-PCR using PlatiniumÒ Taq DNA High Fidelity Polymerase (Invitrogen) and the primers: PDZ-1-2, forward: 5¢-CCGAATTCGAAGAAATCACACTTGAAAGG-3¢, and reverse: 5¢GGATCCCCATCATTCATATACATACTTGT GGGTT-3¢;...

Ngày tải lên: 22/03/2014, 16:20

... pcDNA3.1-GST-Nur77 plasmid pSilencer-shNur77 was prepared by overlapping strategy with primers 5¢-gacGGATCCgcagtccagccatgctccttt caagagaaggagcatg-3¢ (with BamHI), 5¢-cggAAGCTTtATC GATccaaaaaacagtccagccatgctccttctcttg-3¢ ... 5¢-GACTCGCAGACAATGATGG TC-3¢ and 5¢-GCAAACTCATCATGGGCACC-3¢ The results were normalized with b-actin, for which the primers were 5¢-ATGGTGGGAATGGGTCAGAAG-3¢ and 5¢-CA CGCAGCTCATTGTAGAAGG-3¢ Another ... CCTCCAAAAAGCACACAGA-3¢ for St-182 and St-93/ -182, 5¢-AGAAATTATCATCTTTTCCAGTCCGAGA-3¢ for St-93 and St-93/-182, and 5¢-TGGTCTTGAACTCCT CGTGATCTGCCCA-3¢ for Lst-595 pcDNA3.1-Nur77 expression plasmid...

Ngày tải lên: 23/03/2014, 07:20

... 2589 R sapE–F 2590 F sapE–R 2590 R mopB–F 3103 F mopB–R 3103 R GGCAACGAGCAAGGTCCGAAG AAGTCGTTGCAATCGGCGTCG GGCAACGAGCAAGGTCCGAAG GACGTCGTGAGTGCCTCCGTG CGACGTGCAGTATTACTTTTCTAGGG AGTATCAAACCGTGCTGGTCTCC ... processing would lead to a mature protein of 732 amino acids with a theoretical molecular mass of 78 kDa A search in the PROSITE database of protein families and domains [12] with MCA2590 revealed two ... immunoblot analyses (B) of proteins obtained during the fractionation of M capsulatus Samples of each step during the fractionation procedure were collected and comparable samples (10 lL of each mL fraction)...

Ngày tải lên: 23/03/2014, 11:20

... 29 Masugi, F., Ogihara, T., Hasegawa, T., Sagakuchi, K & Kumahara, Y (1988) Normalization of high plasma level of ouabainlike immunoreactivity in primary aldosteronism after removal of adenoma ... inhibitor of the sodium pump, has been identified in blood plasma, adrenal glands, and the hypothalamus of mammals The adrenal gland as a source of ouabain The surprising observation that a plant toxin ... Komiyama, Y., Nishimura, N., Nishino, N., Okuda, K., Munakata, M., Kosaka, C., Masuda, M & Takahashi, H (1998) Purification and characterization of ouabain-binding protein in human plasma Clin...

Ngày tải lên: 24/03/2014, 00:21

... total RNA using 100 ng random hexamer (Pharmacia, Uppsala, Sweden) The PCR primer sequences used were as follows FasL (forward: 5¢-ATGTTTCAGC TCTTCCACCTACAGAAGGA-3¢, reverse: 5¢-CAGAGA GAGCTCAGATACGTTGAC-3¢); ... substrate 4-methyl-lumbellifery-b-galactoside, and was normalized for protein content [30] A one-way analysis of variance was performed using GraphPad INSTATÒ (GraphPad Software, San Diego, CA, USA) ... 12-myristate 13-acetate (PMA) and CsA were purchased from Sigma Ionomycin was obtained from Calbiochem (La Jolla, CA, USA) All other chemicals used were of the purest grade available from Sigma RT-PCR...

Ngày tải lên: 24/03/2014, 03:21

... ATGAAGAAAAAATAAATAAGC-3¢; and mm0632rev, 5¢-ATGGTAGGTCTCAGCGCTGGCTTTCCAGACGCA TTTTTTGC-3¢ The gene mm0632 was cloned via BsaI restriction sites in plasmid pASK-IBA3 (IBA GmbH, Gottingen, Germany), ... of a monofunctional catalase from Methanosarcina barkeri Arch Microbiol 171, 317–323 39 Brioukhanov A, Netrusov A, Sordel M, Thauer RK & Shima S (2000) Protection of Methanosarcina barkeri against ... hyperthermophilic archaeons and bacteria such as Thermotoga maritima, P furiosus and A fulgidus [5,28] In addition, homologs were found in close relatives of M mazei, such as Methanosarcina acetivorans and...

Ngày tải lên: 28/03/2014, 23:20

... view of focus of attention a word is treated as having a static semantics However metaphor can make the semantic type of objects more flexible By using a verb that only applies to humans, as above, ... terms of a moving vehicle at the same time emphasizing the effort that it takes to leave (as in section 3.3) A party can be described, via "is -a" links of the abstraction hierarchy, as a human process ... level of abstraction, where the key role (termination) is specified, we can search for a candidate metaphorical domain that shares this ancestor There are several possible metaphorical domains that...

Ngày tải lên: 31/03/2014, 06:20