... (Tiedemann et al 1987) At a catchment scale, the ef®ciency of delivery of runoff and drainage water from ®eld to stream may be different in grazed areas compared to slurry spreading areas, and connectivity ... possible, because only single samples were taken each week However, it is clear that the E coli counts in water draining from grazed ®elds, especially the `large drain' sample, were greater than water ... 2001, and two were left ungrazed On one of the grazed paddocks, one of the lambs had to be removed because of sickness shortly after the start of the experiment Faecal samples from of the lambs were...
Ngày tải lên: 15/03/2014, 23:20
... proof of the next lemma follows from that of Lemma 3.1, more technical considerations are needed because p(b) depends on x(b) in this case In particular, the apriori estimate for solutions of ... existence and uniqueness results are established The obtained results extends some results of [2, 4] to infinite dimensional spaces The technique used in this paper follows that of developed in ... systems (1.1) and (1.4) are equivalent in finite dimensional spaces since we may let A( t) ≡ without loss of generality However, in infinite dimensional spaces, (1.1) is more general than (1.4) because...
Ngày tải lên: 21/06/2014, 22:20
How Do Earnings Change When Reservists Are Activated - A Reconciliation of Estimates Derived from Survey and Administrative Data docx
... Office of the Secretary of Defense OSD/P&R Office of the Secretary of Defense–Personnel and Readiness OSD/RA Office of the Secretary of Defense–Reserve A airs RA Reserve A airs RMC Regular Military ... with administrative data NOTES: The percentage difference explained is equal to the negative of the percentage difference between the baseline discrepancy and the discrepancy after the adjustment ... answers Any estimate of tax advantage from the survey would be problematic and not exactly comparable to the estimate from the administrative data Adding a flawed estimate of the tax advantage to...
Ngày tải lên: 23/03/2014, 02:20
Báo cáo khoa học: A peptide derived from cyclin-dependent kinase activator (p35) specifically inhibits Cdk5 activity and phosphorylation of tau protein in transfected cells pdf
... assay using histone H1 as a substrate Lane 1, nontransfected cells; lane 2, roscovitine treated cells; lane 3, transfected with CIP Data represent mean ± SD of three experiments (C and D) The ... Invitrogen, Carlsbad, CA, USA) and anti-T7 Tag monoclonal antibody (Novagen, San Diego, CA, USA) A pcDNA/Amp eukaryotic expression vector and LipofectAMINE Reagent were purchased from Invitrogen (Carlsbad, ... rhodamine-labeled goat anti-(rabbit IgG) secondary antibody for h at room temperature This was followed by three washes with NaCl/Pi, and then the cells were embedded in aqueous medium Fluorescent...
Ngày tải lên: 23/03/2014, 21:21
Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx
... tachycardia), and VF (ventricular fibrillation) appeared were noted and recoded; the cumulative aconitine dosage to induce PVC, VT, VF was calculated Data are expressed as mean ± SEM Differences between ... failure However, the cardiovascular effects of BmK venom have not been systematically studied and a regimen for effective treatments has not been established The mechanisms underlying these alterations ... Beckman amino acid analyzer An Applied Biosystems 47 6A sequencer was used for automated Edman degradation The phenylthiohydantoin derivatives of the amino acids were identified using an Applied...
Ngày tải lên: 31/03/2014, 09:20
Báo cáo sinh học: "In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pot
... a All markers were determined by flow cytometry and data represented are from a single experiment and the Results were confirmed in at least two independent experiments Berencsi et al Journal ... Representative data from patients whose T cells were positive for CCR2 expression b CCR2 expression determined by FACS analysis Data represented are from a single experiment and the Results were ... (MP Biomedical Services, Santa Ana, CA) Statistical analyses Differences between experimental and control values were analyzed for significance by 2-sample Student’s t-test Page of 11 Results...
Ngày tải lên: 18/06/2014, 19:20
báo cáo hóa học: " Development and validation of a preference based measure derived from the Cambridge Pulmonary Hypertension Outcome Review (CAMPHOR) for use in cost utility analyses" doc
... there is general agreement that where possible a generic preference based measure of health status based on general population values should be used to calculate QALYs Generic preference based ... individual level modelling First, ordinary least squares (OLS) were used to estimate a mean level model The mean health state values were the dependent variable and the independent varia- Validation ... two measures Table 5: Comparison of predicted and actual values for selected health state classifications: mean level (ML) and random effects (RE) models Health State Actual mean Estimated mean...
Ngày tải lên: 18/06/2014, 19:20
báo cáo hóa học:" In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pptx
... a All markers were determined by flow cytometry and data represented are from a single experiment and the Results were confirmed in at least two independent experiments Berencsi et al Journal ... Representative data from patients whose T cells were positive for CCR2 expression b CCR2 expression determined by FACS analysis Data represented are from a single experiment and the Results were ... (MP Biomedical Services, Santa Ana, CA) Statistical analyses Differences between experimental and control values were analyzed for significance by 2-sample Student’s t-test Page of 11 Results...
Ngày tải lên: 20/06/2014, 03:20
Báo cáo y học: "Different properties of ACPA and IgM-RF derived from a large dataset: further evidence of two distinct autoantibody systems" potx
... first and second ACPA/RF levels and (b) the difference between the first and second ESR/CRP levels – was measured The correlation between change in ACPA levels and change of levels in markers of ... modest decrease in ACPA levels; however, downward seroconversion does not seem to occur Data on IgM-RF seroconversion in early RA are scarce In very early RA, a decrease in the percentage of patients ... increases with age in healthy/non-RA individuals, and its levels are correlated with the acute-phase response Most of these data have emerged from studies of selected populations with small sample...
Ngày tải lên: 09/08/2014, 14:21
a database of protein functional linkages derived from coevolution doc
... there are ten from the Archaea, five from the Eukaryota and the rest are from the Bacteria In total we have computed 18,077,293 links between proteins coded within these genomes As the number of ... Volume 5, Issue 5, Article R35 Bowers et al A final and substantial difference between the respective databases is their ability to generate genome-specific graphs Because the String database uses ... proteins in other genomes interactions Figure The general mechanism of inference for each of the four methods used by the Proteome Navigator The general mechanism of inference for each of the four...
Ngày tải lên: 09/08/2014, 20:20
Báo cáo y học: "A vertebrate case study of the quality of assemblies derived from next-generation sequence" doc
... assemblies shared 12.2 Mbp of the non-reference sequence (a) 454/Newbler (red); (b) Illumina/SOAP (green) Towards an assessment of gene completeness, we evaluated the relative coverage of the ... the reference genome-wide The percentage of test assembly bases aligned to finished BACs, considered to be the highest quality Gene representation Accuracy We estimated accuracy by aligning each ... and gene annotations Importantly, each of these resources was generated from the same DNA source used to generate the NGS data, allowing for a true apples-to-apples assessment of assembly quality...
Ngày tải lên: 09/08/2014, 22:24
báo cáo khoa học: " Molecular characterization of a rice mutator-phenotype derived from an incompatible cross-pollination reveals transgenerational mobilization of multiple transposable elements and extensive epigenetic instability" ppt
... of the 13 genes in expanded young leaf tissue was performed on three batches of independent RNAderived cDNAs with gene – specific primers (see Additional file 5) The relative abundance of transcripts ... constitutes a "biological stress" whereby the genetic and epigenetic stability of the maternal parent genome might be jeopardized, we performed a crossing experiment between rice (served as the maternal ... transfer and/or recombinatory generation of beneficial alleles, recent studies in both plant and animals have revealed that wide hybridization may generate variations by novel means such as rapid...
Ngày tải lên: 12/08/2014, 03:20
Báo cáo y học: "A VLP-based vaccine targeting domain III of the West Nile virus E protein protects from lethal infection in mice" pptx
... age) were purchased from Harlan and allowed to acclimate to the facility for one week before experiments were performed Experiments were approved by the animal ethics committee of the Erasmus ... can be estimated that an average of 50 DIII-C molecules are displayed per vaccine particle presence of Alum as adjuvant One group was immunized only once (day 28) in the presence of Alum The ... nature of the VLP vaccine furthermore ensures a preferential uptake by antigen-presenting cells such as dendritic cells, and thereby an efficient presentation of DIII- as well as AP205 -derived epitopes...
Ngày tải lên: 12/08/2014, 04:20
Báo cáo khoa học: " A geminiviral amplicon (VA) derived from Tomato leaf curl virus (ToLCV) can replicate in a wide variety of plant species and also acts as a VIGS vector" pps
... respective values at 3, 1, and dpi were arbitrarily assigned as 100% The standard deviations shown are based on three independent experiments clear from the results that the levels of accumulation ... tomato leaves agroinfiltrated with VIGS, VA(AC2M), VA(AC4M) and VA(AC2MAC4M) separately and the release of episome at indicated dpi was checked by PCR using internal primers The respective ACTIN ... sites of agroinoculation The plant heights and the internodal distances of the above infiltrated plants were measured and the values are presented graphically as a function of time (dpi) in panels...
Ngày tải lên: 12/08/2014, 04:20
Báo cáo khoa học: "Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China" ppt
... sequences were aligned using ClustaX v1.8 or MegAlign program in the DNASTAR software package Phylogenetic tree was constructed using the Mega software http://www.megasoftware.net/ Ten Chinese genotype ... identities with the other Chinese genotype HEV isolates referenced here, and the highest sequence identity (97.6%) with another Shanghai HEV strain (AB197674) isolated from a Japanese patient who was ... papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately upon acceptance cited in PubMed and archived on PubMed Central yours — you keep...
Ngày tải lên: 12/08/2014, 04:21
Báo cáo y học: " Detection of epithelial to mesenchymal transition in airways of a bleomycin induced pulmonary fibrosis model derived from an α-smooth muscle actin-Cre transgenic mouse" potx
... stimulation We demonstrate herein that increased βgal expression in the lungs of the BLM-treated mice is mainly to be due to the appearance of myofibroblasts in the subepithelial areas of bronchiole and ... We also thank Professor Xiaoping Jian (Guangdong Teacher College of Foreign language and art, China) and Dr Elaina Collie-Dugui (Department of Medicine and Therapeutics, University of Aberdeen, ... Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately upon acceptance cited in PubMed and archived on PubMed Central yours...
Ngày tải lên: 12/08/2014, 16:20
Báo cáo y học: "Impaired nuclear import and viral incorporation of Vpr derived from a HIV long-term non-progressor" docx
... the results of biomedical researc h in our lifetime." Sir Paul Nurse, Cancer Research UK Your research papers will be: available free of charge to the entire biomedical community peer reviewed ... in expression of the β-gal protein Non-infected cells remain colourless due to the lack of Tat expression and subsequent activation of the β-gal gene Data presented depicts relative levels of ... Holt JA, Cooper DA, Miranda-Saksena M, Boadle R, Kelleher AD, Saksena NK: Comprehensive analyses of a unique HIV-1-infected nonprogressor reveal a complex association of immunobiological mechanisms...
Ngày tải lên: 13/08/2014, 05:21
Dielectric behaviours of pb1 3x 2laxtio3 (PLT a) based ferroelectrics derived from mechanical activation
... energy (Ea) increases as the temperature decreases and becomes undefined at the freezing temperature Tf Ea can be visualized as the activation energy for the temperature dependence of polarization ... the team spirit of the research group are always appreciated A special word of appreciation also goes to all my colleagues in the Advanced Ceramics Lab for making the laboratory a memorable and ... the temperature independence of relative permittivity was observed below K (adapted from [25]) It was suggested that if a material undergoes a transition to ferroelectric state at a temperatures...
Ngày tải lên: 04/10/2015, 15:58
Báo cáo khoa học: Novel strategy for protein production using a peptide tag derived from Bacillus thuringiensis Cry4Aa pptx
... · His–4AaCter tag The released · His–4AaCter tag and undigested 4AaCter–TpNs were removed using a Ni-NTA column The 4AaCter–TpNs and the purified recombinant TpNs were analysed by western blotting ... GST–4AaCters Fig Enzyme activities of GST–4AaCters Alkali-solubilized GST– 4AaCters from the inclusions were subjected to CDNB assay Error bars represent the standard deviation of three or more replicate ... the removal of the C-terminal extension [6] In the present study, we developed a Cry4Aa -derived peptide tag that facilitates the formation of protein inclusions of fused proteins We demonstrated...
Ngày tải lên: 29/03/2014, 09:20