báo cáo khoa học: " Molecular characterization of a rice mutator-phenotype derived from an incompatible cross-pollination reveals transgenerational mobilization of multiple transposable elements and extensive epigenetic instability" ppt
Tài liệu hạn chế xem trước, để xem đầy đủ mời bạn chọn Tải xuống
1
/ 12 trang
THÔNG TIN TÀI LIỆU
Cấu trúc
Abstract
Background
Results
Conclusion
Background
Results and discussion
Identification of a mutator-phenotype
Transpositional activation of multiple TEs in the mutator- phenotype and its selfed progenies
Genome-wide genetic and epigenetic instability in the mutator-phenotype
Correlations between two of the three kinds of instabilities, genetic variation, epigenetic variation, and TE activity
Heritable alteration in expression state of genes encoding for putative DNA methyltransferase, 5-methylcytosine DNA glycosylase and siRNA pathway-related protein in the mutator-phenotype and its progenies
The mutator-phenotype was not caused by parental heterozygosity or contamination, but resultant from the alien pollination-imposed stress
Conclusion
Methods
Plant materials
DNA gel blot analysis
Transposon-display (TD) and PCR-based locus assay on mPing excision and insertion