6 non epigenetic and epigenetic regulation of interleukin 1b expression in vitro
Báo cáo y học: "Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration" ppt
... the addition of IL-1β in either normal or degenerate samples Effect of tumour necrosis factor-alpha on expression of interleukin- 1 and interleukin- 1 receptors The addition of recombinant TNF-α ... gene expression in malignant carcinoma via TNF-R1 [25], and TNF-R1 but not TNF-R2 has been shown to be important in inflammatory responses involving IL-1β and MMP-3 and -9 in murine brain injury ... Stimulation of NP cells by the addition of TNF-α resulted in the upregulation of TNF-α and TNF-R2 in both normal and degenerate cells, although the levels of TNF-R1 remained unchanged This finding is in...
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báo cáo khoa học: " Trust and the regulation of pharmaceuticals: South Asia in a globalised world" ppt
... organisations Ministry of Finance Ministry of Chemicals & Fertilizers Central board of excise and customs Ministry of Law & Justice Foreign MNCs OPPI NIPER Ministry of Commerce &industry, Dept of Industrial ... reasons for a declining trend of trust in medical institutions in the US and also in the UK: the influence of television and other media on public opinion, the fragmentation of community, the ... was designed to integrate the insights of discussions in anthropology of global assemblages and in political economy of global commodity chains; and by integrating ‘bottom-up’ and ‘top-down’ approaches...
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Báo cáo y học: "DHEA-dependent and organ-specific regulation of TNF-α mRNA expression in a murine polymicrobial sepsis and trauma model" pps
... types by increases in cytokine secretion after DHEA treatment [21-23] The main focus of this study comprised the role of DHEA in specifically regulating cytokine expression at the mRNA level in the ... calculating the quotient of the amount of TNF-α mRNA against the amount of mRNA of the housekeeping gene GAPDH * P ≤ 0.05 The data are expressed as mean ± standard error of the mean (b) In lung ... determined [34] Plasma levels of TNF-α peak a few hours after a traumatic or septic insult and decline afterwards At the time points determined in this study (48 hours and 96 hours after sepsis induction),...
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Báo cáo Y học: Regulation of a1,3galactosyltransferase expression in pig endothelial cells Implications for xenotransplantation doc
... promoter is independent of TATA-binding-protein-associated factors and initiator-binding proteins Mol Cell Biol 17, 2973–2984 32 Weis, L & Reinberg, D (1992) Transcription by RNA polymerase II: initiator-directed ... for E-selectin expression (about 300-fold increase in pPAECs vs 3-fold in PEC-A) It remains to be investigated to what extent changes in endothelial levels of a1,3GalT and other terminal glycosyltransferases ... expressed transcript in PEC-A cells (80–90%) and Fig Kinetics of a1,3GalT mRNA isoforms induction in pPAEC (A) and PEC-A cells during TNFa activation (B) The expression of a1,3GalT after stimulation...
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Báo cáo khoa học: The C-terminal region of the proprotein convertase 1⁄ 3 (PC1⁄ 3) exerts a bimodal regulation of the enzyme activity in vitro pdf
... tethering, thus insuring cell-surface anchoring [37] In other cases, the CT-peptide contains integral transmembrane motifs affecting the sorting and recycling of furin and PC5 ⁄ 6B [38–40] In the ... purification of recombinant mPC1 ⁄ and hfurin Recombinant murine PC1 ⁄ was produced using the baculovirus expression system in Sf9 insect cells [7] or through intracoelemic injection in insect larvae ... is explained by the role of C-terminal determinants in allowing proper secretion, correct folding or targeting Nevertheless, a cooperative inhibitory interaction between N- and C-terminal propeptides...
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Báo cáo lâm nghiệp: "Polyamines and ethylene during of Prunus avium L. in vitro rooting" pps
... The results outlined above suggest that polyamines may be involved in the rooting process, probably in the stages of active cell division In fact, increases in intracellular PUT and SPD levels ... determined The incorporation of labeled PUT, the precursor of SPD and SPM, was evaluated by adding 185 kBq to 100 ml of medium (15 shoots) Shoot portions were extracted on d 2, and 15 and polyamines ... methionine uptake indicated that the compound was rapidly taken up (within 30 min) and reached a plateau around 15 h in all shoot portions Preliminary data concerning ethylene biosynthesis indicate...
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Báo cáo y học: " Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro: role of glutathione" pdf
... concentration of CSC in absence of NAC (Fig 1B) or BSO (Fig 1C) of the samples containing equal amounts of protein were suspended in reducing SDS-PAGE sample buffer and boiled for minutes Proteins were ... obtained after incubating cells with GSH (1.25 – mM) instead of NAC (data not shown) In contrast, in NCI-H292 cells but not in PBEC, depleting GSH using BSO resulted in a full inhibition of wound ... N-acetylcysteine (NAC) on CSC-induced epithelial wound repair in NCI-H292 and in PBEC Effect of3 Effect of N-acetylcysteine (NAC) on CSC-induced epithelial wound repair in NCI-H292 and in PBEC Mechanical...
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Epigenetic regulation of normal and malignant hematopoiesis
... signals (NLS) and HAT domain of MORF, and the RARa-binding domain, CREB-binding domain, bromodomain and HAT domain of CBP, is thought to promote aberrant patterns of acetylation; however since both ... S-adenosylmethionine (SAM) to the e-nitrogen in lysine or the guanidinium nitrogen in arginine These include (1) lysine-specific SET-containing HMTs involved in the methylation of H3K4, H3K9, Epigenetic regulation ... additional HP1 molecules and other chromatin-modifying proteins, thereby spreading and maintaining the heterochromatin state Since then, the involvement of SUV39H1 an SUV39H2 in regulating hematopoietic-specific...
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Regulation of interleukin 12 and interleukin 23 production by tristetraprolin
... Linking innate and adaptive immunity 30 1.3.1 Interleukin- 12 and interleukin- 23 and their receptors 31 1.3.2 Interleukin- 12 and the TH1 lineage 33 1.3.3 Role of interleukin- 23 in ... still involved in virus specific CD8+ CTL responses (Aldridge et al., 2009) 1.3 Interleukin- 12 and interleukin- 23: Linking innate and adaptive immunity In 1986, Mosmann, Coffman and colleagues ... resulted in the reversal of systemic wasting and inhibition of elevated serum levels of proinflammatory cytokines On the contrary, neutralization of IL-23 resulted in the reduction of intestinal...
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Epigenetic potential of histone deacetylase inhibitors in treating fibroproliferative diseases and preventing peri implantational fibrosis
... methyltransferases and methylated CpG binding proteins, such as methyl-CpG binding protein (MECP2), methyl-CpG binding domain protein (MBD) 1, MBD2 and MBD3, resulting in histone deacetylation and transcriptional ... Furthermore, proinflammatory cytokines interleukin (IL)-1 and IL-6, chemokines IL-8, monocytes chemotactic protein (MCP-1), macrophage inflammatory protein 1α (MIP-1α), Th1 cytokines interferon γ (INFγ), ... Cellular effects of HDACis The main effects of HDACis involve in inducing cell cycle arrest in tumor and normal cells, apoptosis and terminal differentiation in tumor cells In addition, HDACis...
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INTERLEUKIN 6 RELEASE FROM t98g HUMAN GLIAL CELL LINE AS a PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN
... Peroxidase xiv IL-1 Interleukin- 1 IL-1β Interleukin- 1β IL-6 Interleukin- 6 IL-8 Interleukin- 8 IL-10 Interleukin- 10 IL-18 Interleukin- 18 i.t Intrathecal L-PGDS Lipocalin-type Prostaglandin D Synthase ... are involved in the early development of chronic pain, while astrocytes function in sustaining the pain (Vallejo et al., 2010) An interesting finding was that nerve injury induces an increase in ... neuropathic pain 1.2.3 Pro-inflammatory Cytokines The role of pro-inflammatory cytokines, namely tumour necrosis factor-α (TNF-α), interleukin- 1β (IL-1β) and interleukin- 6 (IL-6), in the initiation and...
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
... SD induced significant translational upregulation of pro-IL -1b and that processing of pro-IL -1b into mature IL -1b requires activating cleavage of pro-caspase [23], the cleavage of both pro-IL -1b ... stimulatory signal in order to secrete IL -1b and TNF-a Hypoxia ⁄ SD induces the transcription and secretion of IL-10 Because of the lack of secretion of the in ammatory cytokines IL -1b and TNF-a from ... transplantation in rat models of myocardial infarction has anti -in ammatory effects, decreasing protein production and gene expression for IL -1b and TNF-a [11] To address these paradoxes of both pro- and...
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Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx
... start of exon resulting in loss of the AUG initiating translation of the 88 kDa isoform We specifically point out that differential utilization of exon vs exon in splice variants introduces differing ... staining patterns (comparing and 2) and by the yellow color resulting from combination of green and red fluorescence in that iPLA2c is localized, in large part, to the mitochondrial compartment in ... Translational regulation of iPLA2c in myocardium Owing to the obvious complexity of the regulation of iPLA2c resulting from the combined presence of transcriptional and translational regulation, ...
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Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf
... overexpression and are also found with other TRAF proteins containing a RING– zinc finger domain (data not shown and Fig 6) Interestingly, both a C-terminal deletion mutant lacking the TRAF domain ... cytoplasmic retention of TRAF4 Interestingly, it has been recently shown that the oncogenic serine–threonine kinase Pim-1 induces translocation of the TRAF4-interacting protein-sorting Nexin 6–TRAF4–associated ... TRAF domain of TRAF2 and TRAF3 but were detected regularly in cells transfected with TRAF2/3 deletion mutants consisting of the RING–zinc finger domain Since TRAF proteins tend to form homo- and/ or...
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Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
... against a sequence between the N-terminal and the first transmembrane domain of the protein of the human and rat CB2 receptor The specificity of the CB1 and CB2 antibodies was described in McIntosh ... depending on their lineages and stage of differentiation [53] Indeed, the expression of cannabinoid Ó FEBS 2002 CB1 and CB2 receptors in immune cells appears to be regulated by LPS, cytokines and ... higher than those of anandamide In fact, after LPS-induced maturation, the amounts of 2-AG were increased 2.8-fold, as in the mouse macrophage J774 cell line [24] and in rat circulating macrophages...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
... N-terminal half of the protein, including the dehydrogenase domain Specific binding of the FNR is mediated by a unique series of proline/serine-rich repeat motifs located in the C-terminus For the integration ... in protein–protein interaction are blue and the dehydrogenase domains of Tic32 and Tic62 are shown in green Tic110 contains two transmembrane domains at the proximal N-terminus The topology of ... concerning the role of Tic40 and Hsp93 in the motor complex [27,28,32–34] and the possible order of events [35] Tic40 is an integral membrane protein containing a single transmembrane span within...
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Báo cáo khoa học: Regulation of matrix metalloproteinase activity in health and disease pdf
... step of the activation by binding to the 64 kDa intermediate form of MMP-2 [52] Binding and activation of MMP-2 was abrogated in the presence of avb3 integrin-binding macromolecules such as vitronectin ... illustrated in Fig 2B (step 1) Interactions with small integrin-binding ligand N-linked glycoprotein (SIBLING) Individual members of the SIBLING family are known to bind strongly to both pro- and active ... proMMP-binding partners involved in activation Allosteric activation Gelatinase interactions with collagen and gelatin Binding of macromolecules or specific thiol-binding reagents to an MMP with an intact...
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Báo cáo khoa học: MicroRNAs and the regulation of fibrosis potx
... severe fibrosis and heart failure [27] and by studies showing that miR-133 and miR-590 are down-regulated in a canine model of nicotine-induced atrial interstitial fibrosis [28,29] Interestingly, mechanistic ... using human lung fibroblasts, has shown that miR-155 expression was increased following treatment with TNF-a and interleukin- 1b and reduced following treatment with TGF-b Mechanistic studies indicated ... reduced expression of miR-29a and miR-29b has been demonstrated during activation of primary rat HSCs, in biopsies of patients with hepatitis C virus infection and in a rat model of fibrosis induced...
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Báo cáo khoa học: Apoptosis and autophagy: Regulation of apoptosis by DNA damage signalling – roles of p53, p73 and HIPK2 ppt
... of caspase-6 in response to adriamycin-induced apoptosis Caspase-6 cuts off the C-terminal negative-regulatory domain of HIPK2, which results in a hyperactivated truncated HIPK2 isoform and increased ... an increased SUMOylation and stability of PML, suggesting an additional role of HIPK2 in regulating DNA damageinduced cell death Collectively, these findings indicate that HIPK2 is involved in ... Homeodomain-interacting protein kinase-2 phosphorylates p53 at Ser 46 and mediates apoptosis Nat Cell Biol 4, 11–19 Dauth I, Kruger J & Hofmann TG (2007) Homeodomain-interacting protein kinase is...
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Báo cáo Y học: DNA supercoiling in Escherichia coli is under tight and subtle homeostatic control, involving gene-expression and metabolic regulation of both topoisomerase I and DNA gyrase docx
... the difference in linking number of pBR322 in the respective sample and of pBR322 isolated from cells incubated for 30 with 0.1 mgámL)1 of coumermycin and 0.2 mgámL)1 rifampicin Topoisomerase ... esupercoilingI and esupercoiling , re¯ecting the sensitivity of transcription of topoisomerase I and DNA gyrase for DNA supercoiling Certain simpli®cations were made in [25], i.e grouping of transcription ... ®ts in Fig to an aLk of (corresponding to coumermycin inhibition) indicates a 2.8-fold induction Fusion of the topA promoters to the galactokinase gene showed a twofold to fourfold inhibition of...
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