4mechanical loading of neural cell and tissue models
Báo cáo sinh học: "Altering a-dystroglycan receptor affinity of LCMV pseudotyped lentivirus yields unique cell and tissue tropism" docx
Ngày tải lên :
14/08/2014, 19:22
... accuracy of automated measurements of transduction efficiency Cell types were determined by examining nuclear and cellular morphology Results Developmental expression of a- and b-DG in liver tissue ... investigate the expression of a- and b-DG in the liver at pre- and postnatal time points and document the in vivo tissue tropisms of these LCMV pseudotypes in neonatal and adult mice We hypothesized ... isolates WE54, LCMV Cl13, and Traub efficiently target antigen presenting cells (dendritic cells) in the spleen and perturb their ability to present antigen to T cells and B cells, resulting in a...
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Báo cáo khoa học: Intermittent hypoxia is a key regulator of cancer cell and endothelial cell interplay in tumours pot
Ngày tải lên :
30/03/2014, 04:20
... Durand & AquinoParsons that blood flow decreases could transiently arrest the division of tumour cells in S-phase [62] These cells are the main targets of chemotherapy, and the arrest of their cell ... hypoxia on cancer cells and endothelial cells within a tumour Fig Schematic representation of the effects of HIF-1 activation under intermittent hypoxia on cancer cells and endothelial cells within ... targets of these treatments comprises the endothelial cells One endothelial cell can control the survival of approximately 50–100 tumour cells [93] Therefore, the destruction of a few endothelial cells...
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cell and tissue culture
Ngày tải lên :
10/04/2014, 11:12
... NIH3T3 Cell line HeLa Cell line J558L Cell line Vero Myeloma cell lines Hybridomas Cell line MRC-5 Cell line WI-38 Cell line Namalwa Cell line BHK-21 Cell line MDCK Cell line GH3 Cell line 293 Cell ... calculation cell densities of Cell culture §ubculture of suspension cells Subculture of adherent cells Rejection of failed cell cultures Cryoprese~ation i arations of sterile filtered D Preparation of ... standard ~ Q R CELL, BANKS ~ K ~ ~ plan study of a ~ r i correction ting and Preparation and §tructure definition of project of SOPS Fumigation of tissue culture cabinets and the cell banking laborato~...
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introduction to cell and tissue culture
Ngày tải lên :
11/04/2014, 09:48
... INTRODUCTION TO CELL AND TISSUE CULTURE: Theory and Technique Jennie P Mather and Penelope E Roberts Page iii Introduction to Cell and Tissue Culture Theory and Technique Jennie P Mather and Penelope ... study of cellular physiology and metabolism, and the growth of normal and transformed human cells in vitro (Hayflick and Moorehead, 1961; Leibovitz, 1963; Puck and Marcus, 1955) Arising out of this ... the turn of the century formed with cells, including production of genetically altered cell lines through mutagenesis and cloning, direct comparison of cells from normal and transformed tissues,...
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ANL DIS 13 05 review of existing hydro and PSH models
Ngày tải lên :
16/04/2014, 01:48
... length of the penstock as defined above and a is the wave velocity The wave velocity is a function of the properties of water and of the material the penstock is made of as well as the diameter and ... advantages of recent advances in the design of pumped storage hydro plants The objective of the first task of this project, “Develop Prototype Models of Advanced Pumped Storage Hydro (PSH) and Conventional ... review of the status of hydro unit modeling in the commercially available software packages used by utilities and system operators in the planning and operation of the U.S power grid The two software...
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analysis and stochastics of growth processes and interface models oct 2008
Ngày tải lên :
11/06/2014, 07:05
... List of Contributors Introduction PART I ix QUANTUM AND LATTICE MODELS A Quantum and Lattice Models Directed random growth models on the plane Timo Sepp¨l¨inen aa The pleasures and pains of studying ... macroscopic models Quantum and lattice models Random growth models Random growth models describing the evolution of an interface in the plane are discussed by Sepp¨l¨inen For specific models, three ... Analysis and Stochastics of Growth Processes and Interface Models This page intentionally left blank Analysis and Stochastics of Growth Processes and Interface Models Edited by Peter...
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Báo cáo y học: " Chitosan Interferon-c Nanogene Therapy for Lung Disease: Modulation of T-Cell and Dendritic Cell Immune Responses" pps
Ngày tải lên :
08/08/2014, 21:20
... U/mL) in the presence of DNase I (200 mg/mL) and passed through a 40-micron cell strainer to prepare single -cell suspensions This is a standard method of cell preparation and does not result in ... in the range of to million per gram of lung tissue The CD11c+ cells were then seeded into 12-well plates at 105 cells per well and cultured for 48 hours in the presence of 10 mg of chitosanpVAX ... maceration of the lymph nodes through 40-micron cell strainers, and CD11c+ cells were isolated by magnetic bead separation The cells were stained for CD11b and separately for each of the dendritic cell...
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Báo cáo y học: "Early rheumatoid arthritis is characterized by a distinct and transient synovial fluid cytokine profile of T cell and stromal cell origin" pptx
Ngày tải lên :
09/08/2014, 06:22
... best of a subset of predictors randomly chosen at that node Onethird of the cases are left out of each bootstrap sample and not used in the construction of any given decision tree Each of these ... aspirations ANA, JML, CG and CB participated in the design of the study and interpretation of data MS participated in the design of the study and interpretation of data, and was involved in drafting ... by significantly elevated levels of T cell related cytokines (IL-2, IL-4, IL-13 and IL-17) and stromal cell and macrophage related cytokines (EGF, bFGF, IL-1 and IL-15) when compared with synovial...
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báo cáo khoa học: "Public perception of stem cell and genomics research" ppt
Ngày tải lên :
11/08/2014, 12:21
... line is this: combining stem cell and genome technologies is a terrific idea I foresee a deeper understanding of human development and disease as a result of this union and, thus, a shorter path ... expectations for the fruits of stem cell and personal genomics research are high but the general understanding of each, particularly their limitations, is low This gap in public understanding is a particular ... C: Do Scientists Understand the Public? Cambridge, Massachusetts: American Academy of Arts and Sciences; 2010 11 Murdoch CE, Scott CT: Stem cell tourism and the power of hope Am J Bioeth 2010,...
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Báo cáo y học: "Identification of target antigens of antiendothelial cell and anti-vascular smooth muscle cell antibodies in patients with giant cell arteritis: a proteomic approach" potx
Ngày tải lên :
12/08/2014, 17:22
... pathophysiology of GCA and the ongoing inflammatory process in the arterial wall The combined use of 2-DE and immunoblotting offers an interesting approach to the identification of target antigens of autoantibodies ... the proteome of VSMCs and HUVECs are present in the sera of patients with GCA These antibodies recognise cellular targets that play key roles in cell biology and the maintenance of homeostasis ... for one representative sera pool of patients (top) and the pool of HCs (bottom) (C) Proteome of human umbilical vein endothelial cells showing the localisation of vinculin spots displayed in (A)...
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Báo cáo y học: "Early release of high mobility group box nuclear protein 1 after severe trauma in humans: role of injury severity and tissue hypoperfusion" pptx
Ngày tải lên :
13/08/2014, 19:20
... of coagulation and of the protein C system and the release of other markers of inflammation and endothelial activation early after trauma Here, we report an extracellular release of HMGB1 within ... severity of injury, tissue hypoperfusion, activation of the protein C system and coagulation abnormalities, complement activation and the release of other biomarkers of endothelial cell activation ... part of the standard management of major trauma patients The base deficit was used as a measure of the degree of tissue hypoperfusion Admission base deficit is a clinically useful early marker of...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
Ngày tải lên :
19/02/2014, 16:20
... iron loading of the liver epithelial cell line HepG2 influenced the expression of immune regulatory molecules known to function as ligands of selected subsets of T cells, such as MHC class I and ... detectable after week of culture ( 20% of cells positive for iron) and reached a plateau after weeks of culture ( 60% of cells positive, Fig 2A) In all subsequent experiments, HepG2 cells were cultured ... cytometry revealed that the inhibition of cell growth was due to a decrease in the percentage of cells in the S and G2 ⁄ M phases of the cell cycle (Fig 3B) In a total of seven separate determinations,...
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Báo cáo hóa học: " Comparison of T-cell receptor repertoire restriction in blood and tumor tissue of colorectal cancer patients" docx
Ngày tải lên :
18/06/2014, 16:20
... multitude of SDj of this fam- comparing repertoire restriction of tissue with blood ily CDk of a sample was defined as sum of the moduli of Per-family normalized relative concentration P'jk of a fam- ... average given in fold of standard deviation of the respective family (C) The sum of the modulus' normalized concentrations of all families of the samples (CD) and the number of families elevated ... repertoire in tissue samples of CRC patients Twenty-four samples of normal colon tissue and 18 carcinoma samples were evaluable The statistical analysis of both CD and n(F) comparing all tissue samples...
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Báo cáo y học: " Single cell studies of the cell cycle and some models" pps
Ngày tải lên :
13/08/2014, 22:22
... along with a great deal of skill and hard work In one set of experiments on single cells [17], they made a measurement of the dry mass of single cells by interferometry and then placed it in the ... eukaryotes Models My title makes mention of "some models" Let me be clear that there are two quite different types of cell cycle models One type includes detailed mathematical and molecular models ... rate of cell growth and rate of DNA synthesis in Escherichia coli B/r Proc Natl Acad Sci USA 1971, 68:1061-1064 Prescott DM: Relations between cell growth and cell division Reduced weight, cell...
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Báo cáo y học: "Developmental stage related patterns of codon usage and genomic GC content: searching for evolutionary fingerprints with models of stem cell differentiation" potx
Ngày tải lên :
14/08/2014, 20:22
... Descriptions and definitions of each cell type in the models of stem cell differentiation Model Descriptions Definitions Pluripotent stem cell C57Bl/6 cell line A Adult neural stem cell *Neurosphere ... developmental stages in two models of stem cell differentiation Cell types of different developmental stages in two models of stem cell differentiation (a) Cell types of earlier developmental stages ... (hematopoietic stem cells (HSCs)) and neural (neural stem cells (NSCs)) tissues, as well as the main cell populations in bone marrow (BM) and the cells in lateral ventricles of the brain (LVB),...
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Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
Ngày tải lên :
09/09/2015, 18:56
... msiPSCs and MEFs 58 10 LIST OF FIGURES Figure Generation of iPS cells from mouse embryonic fibroblasts (MEFs) 55 Figure Generation of neural stem cells from mouse iPS cells and characterization ... pluripotent stem cell “stemness”-specific and neural stem cell- specific markers 57 Table Changes in expression of embryonic stem cell- specific markers and embryonic stem cell differentiation/lineage ... 1.3.5 Current status of gene therapy for glioma and breast cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells 1.5 Stem cell as a vehicle...
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Identification of novel cytosolic binding partners of the neural cell adhesion molecule NCAM and functional analysis of these interactions
Ngày tải lên :
25/11/2015, 13:27
... intracellular domain Intracellular domain of human NCAM isoform 140 Human NCAM isoform 180 Intracellular domain of human NCAM isoform 180 Extracellular domain of human NCAM Intracellular domain of ... of the samples and standard dilutions were pipetted in triplicates into wells of a 96-well microtiter plate and incubated with 25 àl of a mixture of solution S and A (ratio 1:50) and 200 àl of ... Introduction 1.1 Cell adhesion molecules Cell adhesion is crucial for the development and maintenance of tissue structures and multicellular organs In mammals, several families of cell adhesion...
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A CFD analysis of transport phenomena and electrochemical reactions in a tubular-shaped PEM fuel cell
Ngày tải lên :
05/09/2013, 14:58
... were independent of the grid size A computational quadratic mesh consisting of a total of 11371 nodes and 60778 meshes for planar cell and 16927 nodes and 94547 meshes for tubular cell were found ... onset of pore plugging, which has a detrimental effect of the fuel cell performance This model is used to analyse and evaluate the performance of a planar and a tubular-shaped PEM fuel cell 2.1 ... distribution of reactant species in this design of the perforated type gas distributor One of the new architectures in PEM fuel cell design is a tubular-shaped fuel cell Fuel cell companies and research...
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Tài liệu Macro-Finance Models of Interest Rates and the Economy pdf
Ngày tải lên :
17/02/2014, 03:20
... that the recent financial and economic crisis—the Great Panic and Recession of 2008 and 2009—will both rearrange the economic landscape and affect the focus of economic and financial research going ... zero These loadings ensure that Lt , St , and Ct have a standard interpretation of level, slope, and curvature (The parameter λ determines the exact shape of these loadings.) Diebold and Li (2006) ... set of 8standard parameters, this benchmark model can be solved and responses given Quarters of the term premium and the other variables of the model to economic shocks can be computed Figures and...
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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx
Ngày tải lên :
18/02/2014, 16:20
... relevance The understanding of how cell swelling integrates into the cell cycle machinery and how cell shrinkage sensitizes cells to apoptotic stimuli requires further scientific effort Cell hydration ... general, cell swelling stimulates anabolic metabolism and proliferation and provides cytoprotection, whereas cellular shrinkage leads to catabolism and insulin resistance and sensitizes cells to ... (caveolae), intracellular organelles, ligandindependent activation of growth factor- and cytokine receptors and autocrine stimulation of signal transduction by release of mediators such as ATP [10,11]...
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