3 cellular and molecular analysis of neural development of glycosyltransferase gene knockout mice
MORPHOGENESIS THE CELLULAR AND MOLECULAR PROCESSES OF DEVELOPMENTAL ANATOMY pptx
Ngày tải lên :
05/03/2014, 11:21
... 1 .3 The plan 1 Background 2.1 The past 2.2 Strategies 2 .3 Conclusions 7 14 23 Case studies 3. 1 Introduction 3. 2 Amphibian development 3. 3 Sea-urchin gastrulation 3. 4 Induction 3. 5 The morphogenesis ... 521 36 196 (hb) ISBN 521 436 12 (pb) Morphogenesis I Title II Series QH491.B37 1990, 1992 574 .3' 32-dc20 89-17415 CIP ISBN- 13 978-0-521 -36 196-5 hardback ISBN-10 0-521 -36 196-6 hardback ISBN- 13 978-0-521- 436 12-0 ... consequences of chromosome imbalance: principles, mechanisms, and models L Saxen Organogenesis of the kidney V Raghaven Developmental biology of fern gametophytes R Maksymowych Analysis of growth and development...
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Báo cáo khoa học: Cellular and molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida pptx
Ngày tải lên :
22/03/2014, 15:21
... (NCBI): Gat1 (NP_ 032 166), Gat2 (NP_ 032 167), Gai1 (NP_ 034 435 ), Gai2 (AAH65159), Gai3 (NP_ 034 436 ), GaoA (NP_ 034 438 ), GaoB (P188 73. 3), Gaz (NP_ 034 441), Gas (P 630 94), Gaolf1 (NP_ 034 437 ), Gaolf2 (NP_796111), ... Ga11 (NP_ 034 431 ), Gaq (NP_ 032 165), Ga14 (NP_ 032 1 63) , Ga15 (NP_ 034 434 ), Ga12 (NP_ 034 432 ) and Ga 13 (NP_ 034 433 ) The numbers below the alignment correspond to the amino acid positions of Gaq The ... about the intracellular activity domain, which resides in the C-terminus of each toxin [14, 83, 132 , 133 , 138 , 139 ] The crystal structures of the C-terminal fragments of PMT (PDB 2EBF) [ 139 ] and CNF1 (PDB...
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Báo cáo y học: " Cellular and molecular mechanisms of cigarette smoke-induced lung damage and prevention by vitamin c" ppsx
Ngày tải lên :
11/08/2014, 08:22
... and Bcl-2 of the lung extracts of guinea pigs exposed to air or CS supplemented with A, Immunoblots of caspase 3, PARP, A, Immunoblots of caspase 3, PARP, Bax and Bcl-2 of the lung extracts of ... proteins Methods Enzymol 1994, 233 :34 6 -35 7 Shacter E: Quantification and significance of protein oxidation in biological samples Drug Metab Rev 2000, 32 (3- 4) :30 7 -32 6 Wang X, Thomas B, Sachdeva ... lungs and patients with COPD: morphological impact of VEGFR-2 inhibition Am J Physiol Lung Cell Mol Physiol 2006, 290:L897-L908 http://www.journal-inflammation.com/content/5/1/21 33 34 35 36 37 38 ...
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LIVE TRACKING VIRE2 PROTEIN AND MOLECULAR ANALYSIS OF YEAST FACTOR PMP3P DURING AGROBACTERIUM MEDIATED TRANSFORMATION
Ngày tải lên :
09/09/2015, 11:19
... transformation of plants 39 3. 2 .3 Agrobacterium VirE2 is important in AMT of yeast 40 3. 3 Development of Split-GFP detection system in yeast cells 41 3. 3.1 General strategy of Split-GFP system ... assay of Arabidopsis thaliana 31 2.7 Agroinfiltration 32 Chapter Live tracking of Agrobacterium VirE2 protein in host cells 33 3. 1 Introduction 33 3. 2 General ... URA3, AmpR pST2 03- GFP pST2 03 derivative, expressing GFP, This study URA3, AmpR pST2 03- PMP3-GFP pST2 03 derivative, expressing PMP3-GFP fusion protein from yeast PMP3 promoter and terminator, URA3,...
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Cellular and molecular control of skeleton formation in fish insights from osteoblast ablation and functional characterization of lrp5 and SOst
Ngày tải lên :
10/09/2015, 08:43
... 31 2 .3. 10 Transformation of bacteria 32 2 .3. 11 Preparation of plasmid DNA 33 2 .3. 12 Sequencing of DNA 33 2 .3. 13 In ... rerio), to gain better understanding of the cellular and molecular processes involved in skeletal development To examine the role of osteoblasts in development of the vertebral column, I created ... which develop rapidly and allow reproducibility of experimental settings and real-time analysis of development The transparency of the embryos together with the development of new imaging strategies...
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Molecular analysis of the gene LAS17 mediating t DNA trafficking inside yeast cells
Ngày tải lên :
13/10/2015, 16:50
... detection 33 2.7 Cell Imaging 25 2.7.1 Fluorescent microscopy 34 2.7.2 Confocal microscopy 34 CHAPTER Results and Discussion 3. 1 The role of Las17 gene in Agrobacterium-yeast gene transfer 36 3. 2 The ... and establish the results as shown in Table 3. 1, Figure 3. 1 and Figure 3. 2 38 Transformation Efficiency† (10-5) Folds Differences‡ Experiment Set WT ∆Las17 0.290±0. 03 2.40±0.57 8 .33 6±2. 03 0 .31 4±0.07 ... tumefaciens 24 2 .3. 3 Co-cultivation of A tumefaciens and S cerevisiae 25 2 .3. 4 Recovery and selection of transformants 25 2.4 Lithium Acetate Transformation of S.cerevisiae 27 2.5 PCR Detection of T-DNA...
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Báo cáo khoa học: "Molecular analysis of hprt mutation in B6C3F1 mice exposed to ozone alone and combined treatment of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and/or dibutyl phthalate for 32 and 52 weeks" ppsx
Ngày tải lên :
07/08/2014, 18:20
... (Figs and 2) Analysis of hprt mutations in T-cells from spleens of control and test materials-exposed B6C3F1 mice Analysis of the spontaneous hprt mutant clones yielding The toxicologic actions of ... Interact 1981, 34 , 233 - 238 11 Gray TJ, Beamand JA Effect of some phthalate esters and other testicular toxins on primary cultures of testicular cells Food Chem Toxicol 1984, 22, 1 23- 131 12 Harris ... with 4(N-methyl-N-nitrosamino)-1- (3- pyridyl)-1-butanone and dibutyl phthalate enhanced ozone-induced genotoxicity in B6C3F1 38 5 mice Mutagenesis 2002, 17, 33 1 -33 6 22 Koren HS, Devlin RB, Graham...
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Molecular characterization and developmental analysis of the TGF beta 3 gene in zebrafish
Ngày tải lên :
14/09/2015, 18:03
... 3. 10 Knockdown of tgfb3 Gene and Notochord Development ………………….…… 99 3. 11 Undulating Notochord and Heart Field Domain in tgfb Morphants …… … 102 3. 12 Knockdown of tgfb3 Gene and Heart Development ………………….…….… ... Notochord phenotype in overexpressed tgf 3 (tgf 3 ) embryos Fig. 33 OE Cardiac cones formation in tgfb3 embryos Fig. 34 OE Heart tubes elongation in tgfb3 embryos v Molecular characterization and developmental analysis of ... w, whisker follicles 16 Molecular characterization and developmental analysis of the TGF Beta 3 gene in zebrafish 1.2.1.12 TGFb3 knockout mice studies Mouse knockout studies suggest that Tgfb3 is important ...
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Báo cáo khoa học: Proteomic and biochemical analysis of 14-3-3-binding proteins during C2-ceramide-induced apoptosis pot
Ngày tải lên :
06/03/2014, 22:21
... MDA-MB- 231 14 -3- 3-binding status during apoptosis MDA-MB- 435 EvsaT HeLa M Pozuelo-Rubio 14 -3- 3γ 14 -3- 3ζ 14 -3- 3ε 14 -3- 3σ 14 -3- 3θ Tubulin Fig Analysis of expression levels of several 14 -3- 3 isoforms ... Titin 33 28 Control Ceramide CaM Centrosomal Nek2-associated protein TSC2 Myosin light chain kinase 14 -3- 3f ⁄ d 14 -3- 3e 14 -3- 3c 14 -3- 3h 14 -3- 3g B-cell scaffold protein with ankyrin repeats 14 -3- 3f ... Vimentin VASP B 23 CaM TSC2 B IP: VASP C IP DIG–14 -3- 3 VASP IP: B 23 C IP DIG–14 -3- 3 B 23 IP: TSC2 C IP DIG–14 -3- 3 TSC2 Fig Analysis of phosphorylation-dependent 14 -3- 3 interaction of identified targets...
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The Report of the Task Force on Financial Mechanisms for ICT for Development - A review of trends and an analysis of gaps and promising practices ppt
Ngày tải lên :
15/03/2014, 19:20
... from development finance institutions (FMO of the Netherlands, Development Bank of Southern Africa and DEG of Germany) and US$120-million of senior debt from commercial banks (Barclays Bank plc and ... Intergovernmental Agency of the Francophonie (AIF), the City of Dakar, the City of Geneva, the City of Delémont (Switzerland), the City of Lyon, the City of Paris, the City of Santo Domingo, the City of Curitiba, ... dramatically lowered the cost of goods and services and expanded the range of technology choices and development solutions This in turn has stimulated the entry of new players, principally the...
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Báo cáo khoa học: Molecular analysis of the interaction between cardosin A and phospholipase Da Identification of RGD/KGE sequences as binding motifs for C2 domains pdf
Ngày tải lên :
30/03/2014, 11:20
... 1 131 .52 1444.71 1841. 93 11 03. 54 137 1.65 1175.66 35 58. 63 1175.55 1894.86 1262.66 1 834 . 93 1015.52 2575.11 231 9.10 001–012 019–028 070–081 086–101 172–180 216–228 239 –248 270 30 1 30 6 31 4 37 2 39 0 ... superimposition of the 3D structures of kistrin [32 ] and cardosin A (Fig 8) The importance of both motifs and in particular their basic residues was further emphasized by the complete lack of interaction ... Plant Cell 6, 39 3–404 28 Wang X (2000) Multiple forms of phospholipase D in plants: the gene family, catalytic and regulatory properties, and cellular functions Prog Lipid Res 39 , 109–149 29...
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Báo cáo khoa học: Structural and functional analysis of ataxin-2 and ataxin-3 potx
Ngày tải lên :
30/03/2014, 15:20
... strand) of D3 and I41/L304, C 43/ A306 (both in b3), L69/S326 and L71/L328 (both in b4) of B Stacking interactions between guanidinium groups of arginines R69/V 335 of D3 and R25/G287 and R49/T312 ... residues of D3 and B contribute to the stability of the dimer The first cluster includes F70/V 336 and I72/Q 338 (both in b5 strand) of D3 and F27/Y289 (b2 strand), L67/M324, V70/I327 and L72/L328 (all ... strand) of B The second cluster consists of P6/M267, L10/ L271 (both in a-helix), V18/C279 (b1 strand), L32/F2 93 (b2 strand), I 33/ K294 (loop after b2 strand), I68/F 334 , L71/ V 337 and L 73/ F 339 ...
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Báo cáo y học: " Molecular analysis of HBV genotypes and subgenotypes in the Central-East region of Tunisia" potx
Ngày tải lên :
12/08/2014, 02:20
... Genotypes and phylogenetic characterization of hepatitis B and delta viruses in Egypt 20 03, 70(4):529 -36 doi:10.1186/17 43- 422X-7 -30 2 Cite this article as: Hannachi et al.: Molecular analysis of HBV ... FJ904 433 [ 13- 17] Among the 31 genotype D strains, phylogenetic analysis showed predominance of subgenotypes D1 (54%) A total of 13 samples (41%) clustered into the novel D7 subgenotype group and ... detected in 4% of the cases with specific RFLP profile of A1 pattern (30 1 pb, 121 pb, 57 pb with AvaII and 31 8 pb, 109 pb and 52 pb with DpnII) In 7% of the cases, a restriction profile Hannachi...
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báo cáo khoa học: " Characterization and structural analysis of wild type and a non-abscission mutant at the development funiculus (Def) locus in Pisum sativum L" pdf
Ngày tải lên :
12/08/2014, 03:20
... and meristem arrest in Arabidopsis Plant Cell 1997, 9:1169-1179 26 27 28 29 30 31 32 33 34 35 36 37 Osborne DJ, Sargent JA: Positional differentiation of abscission zones during development of ... 1997, 1 13: 130 3- 130 8 Bonghi C, Tonutti P, Ramina A: Biochemical and molecular aspects of fruitlet abscission Plant Growth Regul 2000, 31 :35 -42 Kalaitzis P, Koehler SM, Tucker ML: Cloning of a tomato ... apparently normal pattern of growth and senescence [26] In Arabidopsis, mutants dab11, dab2-1, dab3-1, dab3-2 and dab3 -3 have all been shown to delay the abscission of floral parts [2] and an abscissionless...
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Báo cáo khoa học: " Molecular characterization and phylogenetic analysis of the complete genome of a porcine sapovirus from Chinese swine" pdf
Ngày tải lên :
12/08/2014, 04:21
... 645-667 5 43- 564 1170-1191 10 63- 1085 1 833 -1854 1 733 -1752 233 5- 235 4 22 23- 22 43 2757-2778 2680-2702 33 40 -33 61 32 43- 3261 38 73- 3891 37 68 -37 92 437 1- 439 4 4261-42 83 4890-4914 4760-47 83 5468-5492 532 8- 535 0 5890-59 13 ... U956 43 AJ606698 AF294 739 AF 435 8 13 AF 435 814 AJ606697 AJ606699 AY2898 03 EU221477 AJ606691 AY289804 AJ249 939 U95645 EU122248 EU122246 EU381 231 DQ05 636 3 AY8264 23 AB242875 AY8 233 08 AY826426 DQ3 832 74 ... AB2428 73 DQ359100 AY974192 DQ359099 AY974195 AB221 130 47.7 42.7 48.5 40 .3 57.1 40.4 50 .3 45.5 43. 8 42.1 29.0 40.0 22.9 43. 2 47.2 88.4 86.1 84.2 86.1 86.1 84.5 82.2 86.7 91.2 20.2 18.6 28 .3 16 .3 29.2...
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Registration, atlas generation, and statistical analysis of high angular resolution diffusion imaging based on riemannian structure of orientation distribution functions 3
Ngày tải lên :
08/09/2015, 19:27
... as kV Id3 3 , where Id3 3 is an identity matrix, such that kV can be a scalar kernel In the rest of the chapter, we shall refer to this LDDMM mapping problem as LDDMM-ODF 3. 3.1 Gradient of J with ... tracts Figure 3. 15 shows the mean square error of spherical harmonics coefficients of these regions across all subjects The mean values of LDDMM-Raffelt is 0.02 73 for CST-left and 0. 031 3 for CST-right, ... 0.001) 3. 5 .3 Computational complexity of LDDMM-FA, LDDMM-ODF and LDDMM-Raffelt The computational complexity for the calculation of the trajectory and the regulation term of LDDMM-ODF and LDDMM-Raffelt...
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Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 3
Ngày tải lên :
09/09/2015, 10:20
... expression and purification (A) Elution profile of nickel-bead affinity and desalting chromatography (B) SDS-PAGE: marker (lane 1), sample before induction (lane 2), pooled desalted fractions (lane 3) , ... fractions (lane 3- 8) Figure 9: ID2 proteins’ purity check by SDS_PAGE: marker (lane M, kDa) N-HLH82-L (gel A, lane 1), HLH24-82-L (gel B, lane 2), HLH24-82-L-Se-Met (gel C, lane 3) ! 38 ! 3. 3 Protein ... results were the same and the identity of the protein was that of ID2 (Table 7) The only difference was that the longer form of ID2, N-HLH82-L contained the intact N-terminal region of the protein as...
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Mathematical and computational analysis of intracelluar dynamics 3
Ngày tải lên :
11/09/2015, 16:05
... Sections 3. 3 and 3. 4 Finally, novel systemic behaviors of the system are inferred and predicted from the simulation results (Sections 3. 5 and 3. 6) 34 3. 2 Formulation of kinetic models Figure 3- 1 Kinetic ... p 53] n1 k [ p 53] v3 = j3n1 + [ p 53] n1 v4 = k [PIP 2] k {[PIPT ] − [PIP3]} = j + [PIP 2] j + [PIPT ] − [PIP3] vm = k m [PTEN ][PIP 3] j m + [PIP3] (3- 3) k [ p 53] n2 v5 = v6 = j5n + [ p 53] n2 k [ ... conserved (Figure 3- 7) p53ss AKT*ss [p53ss] [AKTass ] [AKTT ] Figure 3- 7 Model M3: steady-state bifurcation diagrams of p 53 and AKTa The steady state values of p 53 ([p53ss], gray curve) and AKTa ([AKTass],...
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Expressed sequence tags analysis of major allergens producing dust mites and molecular characterization of their allergens
Ngày tải lên :
12/09/2015, 11:08
... cloned 1 13 3.4 .3 IgE profiles of ESTs homologue to mite allergens reported previously 124 3. 4 .3. 1 Group allergen 124 3. 4 .3. 3 Group and Group 10 allergens 126 3. 4 .3. 4 Group allergens 130 3. 4 .3. 5 Group ... homologues 106 3. 3.1 General analysis of cDNA inserts 106 3. 3.2 Expression vector pET 32 a (+) as choice for recombinant protein expression 107 3. 3 .3 Protein profiles of recombinant allergens 107 3. 4 Immuno-characterizations ... 14 allergen 133 3. 4 .3. 6 Mag 29 allergen 137 3. 4 .3. 7 Group 3, and allergens 140 3. 4.4 Isoallergen homologues for group and group 13 allergen identified in D farinae EST collection 3. 4.4.1 Isoallergens...
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