3 investigative approaches to advance bone tissue engineering

... Biaxial Bioreactor Stimulation of Mesenchymal Stem Cells and Endothelial Progenitor Cells Cocultures in 3D Honeycomb Scaffolds for Vascularised Bone Tissue Engineering Tissue Engineering Part ... International Bone- Tissue- Engineering Congress (Bone- Tec), 7-10 October 2010, Hannover, Germany Y Liu, SH Teoh, SK Chong, Z Zhang, M Choolani, J Chan Human endothelial progenitor cells & bone marrow-derived ... Vascularised Tissue- Engineered Bone Grafts: Endothelial Progenitor Cells in Vasculogenic and Osteogenic Priming of Human Fetal Mesenchymal Stem Cells International Bone- Tissue- Engineering Congress (Bone- Tec),...

Ngày tải lên: 09/09/2015, 10:16

... Biomaterials for Tissue Engineering Bone In: Lee K, Kaplan D, editors Tissue Engineering II: Springer Berlin Heidelberg; 2007 p 275-308 Hutmacher DW Scaffolds in tissue engineering bone and cartilage ... BASED MICROCARRIERS FOR BONE TISSUE ENGINEERING APPLICATIONS ! FENG YONG YAO, JASON NATIONAL UNIVERSITY OF SINGAPORE 2015 ! ! APATITE BASED MICROCARRIERS FOR BONE TISSUE ENGINEERING APPLICATIONS ... for off-the-shelf bone tissue engineering application Biomaterials 2012;33:2656-72 [115] Mauney JR, Volloch V, Kaplan DL Role of adult mesenchymal stem cells in bone tissue engineering applications:...

Ngày tải lên: 22/09/2015, 15:18

... has to be conducted to replace the implant. oLimitations to current bone grafts as discussed propel researchers to look into the field of bone tissue engineering for an ideal bone substitute. 1.1.3 Strategies ... discussed propel researchers to look into the field of bone tissue engineering for an ideal bone substitute 1.1.3 Strategies in BTE Tissue engineering is the restoration, improvement, ... back to the laboratory. They were then vortexed in PBS (containing antibiotics/antimycotic) until the hematopoeitic tissue was removed from the bone chips. The bone chips were dissected into pieces...

Ngày tải lên: 09/10/2015, 11:18

... as ? ?bone tissue engineering? ??. A key component in tissue engineering for bone regeneration is the scaffold that serves as a 3D template for initial cell interactions and the formation of boneextracellular ... of the scaffold to be metabolized by the body allows it to be gradually replaced by cells to form functional tissues [Pollok, 1996]. A well-designed scaffold for bone tissue engineering then ... technically a different bone cell altogether, the osteoblast changes into an osteocyte over time. Osteoblasts have the unique ability to secrete bone tissue and form the tissue around itself like...

Ngày tải lên: 09/10/2015, 11:18

... attributed to secretion of cytokines and growth factors from MSCs [138,144], which might be subjected to batch -to- batch variation [138] The point to be considered is that in most studies locomotor function ... mat to the surrounding tissue was attributed to the astrocytosis and glial scar formation around the implant The attempts to decrease this astrocytosis by incubation of mats with antibodies to ... another strategy that caused ES cells to differentiated into motor neurons lacking Nogo receptor after transplantation into the transected spinal cord of mice and led to functional improvements confirmed...

Ngày tải lên: 19/06/2014, 10:20

... these investigators work together, and such efforts have led to a better understanding of asthma. Too often, however, multidisciplinary research resembles more the parallel play of toddlers than ... diffi cult for our students to achieve their interdisciplinary objectives. Recently, we have worked to develop at CUNY a vari- ety of interdisciplinary approaches to graduate education for social ... at the individual level may be harmful to population health and to social justice. To avoid this unintended effect, urban public health planners need to defi ne disparity reduction as an explicit...

Ngày tải lên: 02/07/2014, 07:20

... deep into the lateral orbit allows retraction of these tissues to expose the frontozygomatic suture Figure 30 Dissection to the level of the frontozygomatic suture The tissues superficial to the ... attaches to the orbital tubercle, to mm posterior to the orbital margin Following canthotomy, the superior limb of the canthal tendon is still attached to the orbital tubercle It is important to place ... conjunctiva to skin, approximately to mm below the lid margin to ensure that the tarsal plate is included in the suture Step Lateral Canthotomy and Inferior Cantholysis If a lateral canthotomy is...

Ngày tải lên: 11/08/2014, 13:20

... potential to be applied to other tissue engineering systems that aim to form tissue constructs to regenerate tissues at sites of critical defects, such as the heart, liver, bone or skin. Acceleration ... the tissue construct prior to implantation. An alternative means of using PHi in tissue engineering applications is to deliver the substances to the site of implantation together with the tissue ... Phosphoglycerate kinase 6-Phosphofructo-2-kinase/fructose-2,6bisphosphatase-3 (PFKFB3) 6-Phosphofructo-2-kinase/fructose-2,6bisphosphatase-4 (PFKFB4) Plasminogen activator inhibitor Procollagen prolyl-4-hydroxylase...

Ngày tải lên: 11/09/2015, 14:29

... 13 2.2.1 Bone tissue engineering 13 2.2.2 Skin tissue engineering . 15 v 2.2.3 Genito-urinary tissue engineering ... Zhu C, Lanctot DR, Agrawal CM, Wang X. Fundamentals of biomechanics in tissue engineering of bone. Tissue Eng 2000 Aug;6(4):361-381. 110. Hutmacher DW. Scaffolds in tissue engineering bone and ... them to fabricate tissue engineered substances Although all tissues were probable candidates of tissue engineering approach, few tissues such as skin, cartilage, and bone are in advanced...

Ngày tải lên: 14/09/2015, 08:48

... found to be differentially expressed by five-fold, with 251 upregulated and 183 downregulated in fetal blood. To identify genes of interest, I then proceeded to use the Gene Ontology Browser to ... telomeres and up to 5.7 fold greater telomerase activity. Based on these findings, I proceeded to study the use of perinatal vascular progenitor cell sources for vascular tissue engineering. UCPVC ... overnight to reduce changes in expression level due to the sorting process. The quality of extracted RNA was assessed using spectrophotometry and formaldehyde gel electrophoresis prior to labelling...

Ngày tải lên: 14/09/2015, 08:49

... in new strategies for producing engineered tissue To select appropriate polymeric scaffold for tissue engineering application, it is important to understand the influence of the polymer ... Copolymers Predetermined amounts of ∈-caprolactone, mPEG or dihydroxyl PEG and zinc powder were introduced into a round-bottomed flask. The caprolactone/ethylene oxide or [CL] / [EO] molar ... stepwise ROP of ε-caprolactone and DL-lactide. In the first step, ε-caprolactone (171 g), mPEG5000 (22 g) and zinc powder (0.05 wt-%) were introduced into a roundbottomed flask. Polymerization...

Ngày tải lên: 14/09/2015, 11:12

... the capacity to differentiate into parenchymal hepatocytes or into bile ductular cells These stem cells may be activated to proliferate after severe liver injury or exposure to hepatocarcinogens ... University of Tokyo, Tokyo, Japan ix x Contributors YASUMASA KURODA Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, Sendai, Japan JOSE MARIA MATO CIC ... able to provide metabolic functions, detoxify toxins, and synthesize proteins in place of the injured liver would be extremely useful in acute liver failure to bridge patients to recovery or to...

Ngày tải lên: 29/05/2014, 17:06

... well understood Hypoxia-inducible factor (HIF)-1 is one of the key transcription factors in the response to hypoxia; it mediates a variety of adaptive cellular and systemic responses to hypoxia ... constructed into the recombinant adenoviral vector The recombinant adenoviral vector, digested with PacI to linearize the plasmid and ethanol-precipitated, was used for transfection into HEK293 cells ... neurospheres (D, E) Flow cytometric analysis showed that hypoxia, especially 10% O2, led to more NPCs in the S phase and G2 ⁄ M phase of the cell cycle (F) Cartogram of flow cytometric analysis Hypoxia...

Ngày tải lên: 30/03/2014, 04:20

... endothelium to respond to physiological cues can alter mental [2], sexual [3], visual [4], and respiratory [5] ability Specifically, minute alterations in the ability of endothelium to respond to neurotransmitter ... found in the bone marrow [73,74] suggests that exhaustion in response to long-term demand may be occurring If the reparatory demands of the injury indeed lead to depletion of EPC progenitors, then ... function Circ Res 2002, 90:E89-93 Ceradini DJ, Gurtner GC: Homing to hypoxia: HIF-1 as a mediator of progenitor cell recruitment to injured tissue Trends Cardiovasc Med 2005, 15:57-63 Schomig K, Busch...

Ngày tải lên: 18/06/2014, 15:20

... of peripheral blood hematopoietic stem cell numbers was believed to be limited to postbone marrow injury conditions [43], subsequent studies have expanded this finding to situations of inflammation ... progenitor cells in fracture healing and distraction osteogenesis Bone 2008, 42(5):932-41 13 Matsumoto T, Mifune Y, Kawamoto A, et al: Fracture induced mobilization and incorporation of bone marrow-derived ... media or (b) an optimized hematopoietic growth factor cocktail as described in Materials and Methods Figure Stem-Kine Supplementation Increases Hematopoietic Progenitor Cells in Circulation PBMC...

Ngày tải lên: 18/06/2014, 16:20

... immunomonitoring will be performed on days 1, 3, 10, and 30, including mixed lymphocyte reactions to evaluate anti-donor reactivity, flow cytometry to describe the recipients’ leucocyte repertoire, ... macroembolism To monitor for potential embolus formation, we have specified diagnostic procedures to examine the liver and lung after intraportal and intravenous injection, respectively Toxicity related to ... Adherent, non-hematopoietic bone marrow stem cells, including MAPCs and MSCs, have been shown to beneficially modulate the anti-donor immune response in organ transplantation and to promote tissue regeneration...

Ngày tải lên: 18/06/2014, 22:20

... immunomonitoring will be performed on days 1, 3, 10, and 30, including mixed lymphocyte reactions to evaluate anti-donor reactivity, flow cytometry to describe the recipients’ leucocyte repertoire, ... macroembolism To monitor for potential embolus formation, we have specified diagnostic procedures to examine the liver and lung after intraportal and intravenous injection, respectively Toxicity related to ... Adherent, non-hematopoietic bone marrow stem cells, including MAPCs and MSCs, have been shown to beneficially modulate the anti-donor immune response in organ transplantation and to promote tissue regeneration...

Ngày tải lên: 20/06/2014, 04:20

... interest relevant to this article was reported We are indebted to the staff of our catheterization laboratories; to Beate Mantz, Isabel Geweyer, and Heike Braun (study nurses); to Tina Rasper (biologic ... the day -to- day editorial activities of the Journal but is expected in addition to have his or her own independent projects Please send curriculum vitae and research interests to the Editor-in-Chief, ... inflammation Triggers leading to atherosclerosis: Risk factors for CAD Local hemodynamic factors (shear stress) Triggers probably leading to focal inflammation: Local hemodynamic factors Local vascular-remodeling...

Ngày tải lên: 27/06/2014, 00:20

... astrocytomas to chemotherapy Cancer Res 2000, 60:4812-4818 Moore-Maxwell CA, Datto MB, Hulette CM: Chemotherapyinduced toxic leukoencephalopathy causes a wide range of symptoms: a series of four autopsies ... growth factor signal transduction J Neurosci 1990, 10:184-193 66 Tomkins CE, Edwards SN, Tolkovsky AM: Apoptosis is induced in post-mitotic rat sympathetic neurons by arabinosides and topoisomerase ... ML, Toda H, Palmer TD: Inflammatory blockade restores adult hippocampal neurogenesis Science 2003, 302:1760-1765 84 Nagahiro S, Yamamoto YL, Diksic M, Mitsuka S, Sugimoto S, Feindel W: Neurotoxicity...

Ngày tải lên: 06/08/2014, 18:21

... progenitor cells identified in OA cartilage have marked similarities to bone marrow MPCs However, as a total population, unlike bone marrow MPCs, cartilage derived cells could not form bone in ... early to late developmental stages This knowledge may provide a basis to improve current approaches of cell based therapies for cartilage defects and to develop new strategies for guided tissue ... histological evaluation, sections were deparaffinized and either stained with haematoxylin or Alcian blue at pH 2.5, with additional Kernechtrot counterstaining, according to standard protocols...

Ngày tải lên: 09/08/2014, 01:23