3 evidence of concentration dependent biological activity of an antineoplastic agent

Báo cáo khoa học: Enhancement of intracellular concentration and biological activity of PNA after conjugation with a cell-penetrating synthetic model peptide docx

Ngày tải lên : 23/03/2014, 13:20

... anticipated, antisense down-regulation of the nociceptin/orphanin FQ receptor to be the mechanism of biological activity of compounds I and II The antisense pretreatment remained without any inﬂuence ... samples ± SEM Energy -dependent and energy-independent mechanisms are involved in the cellular uptake of both naked PNA and its MAP conjugate The cellular uptake of both the naked PNA and the conjugate ... sensitive to lowered temperature and energy depletion, implying the involvement of nonendocytic mechanisms (Fig 3) On the other hand, energy -dependent and -independent mechanisms contributed differently...

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Cecilia vol. 3 Memoirs of an Heiress pot

Ngày tải lên : 07/03/2014, 01:20

... fruitless than various At one moment she imagined it some frolic of Morrice, at another some perfidy of Monckton, and at another an idle and unmeaning trick of some stranger to them all But none of these ... voice of wisdom and experience in the first of moralists, and most enlightened of men, [Footnote: Dr Johnson.] and reading the letter of Cowley, I saw the vanity and absurdity of panting after solitude ... misfortune, who forsees in the rage of her husband, and the resistance of her son, all the misery of domestic contention, and who can only secure the honour of her family by destroying its peace!...

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Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt

Ngày tải lên : 19/02/2014, 08:20

... lysate Lane 1, 2.5 lg of GST-ExoS(wt); lane 2, 1.25 lg of GST-ExoS(wt); lane 3, 0.75 lg of GST-ExoS(wt); lane 4, 2.5 lg of GST-ExoS(L426A); lane 5, 1.25 lg of GST-ExoS(L426A); lane 6, 0.75 lg of GST-ExoS(L426A); ... GST-ExoS(L426A); lane 7, 2.5 lg of GST-ExoS(D427A); lane 8, 1.25 lg of GST-ExoS(D427A); lane 9, 0.75 lg of GST-ExoS(D427A); lane 10, 2.5 lg of GST-ExoS(L428A); lane 11, 1.25 lg of GST-ExoS(L428A); lane 12, ... GST-ExoS(DALDL424–428AAAAA); lane 7, GST-ExoS(D424A; D427A); lane 8, GST-ExoS(S419I); lane 9, GST-ExoS(Q420A); lane 10, GST-ExoS(G421A); lane 11, GST-ExoS(L422A); lane 12, GST-ExoS(L423A); lane 13, GST-ExoS(D424A); lane...

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Tài liệu Activity 4.3: Value of Information Models docx

Ngày tải lên : 10/12/2013, 16:16

... 22 Activity 4.3: Value of Information Models Exercise 1: Identifying the Value of Information Models ! Identify the value of information models Think of how you have used information ... informational modeling provides to the design process and specifically to the analysis step of conceptual design The instructor will write your answers on a flip chart ...

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Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain pdf

Ngày tải lên : 16/02/2014, 15:20

... Regulation of ROS occurs through several ROS scavengers, or antioxidants Antioxidants can be both enzymatic and non-enzymatic, and function to protect cells against oxidative damage Specific antioxidants ... critical for the biological activity of GIF Mutations in this motif, specifically changing the two Pro residues to Ala and Ser (found in human MT2) abolished the neuroinhibitory activity of GIF [33] ... survival of cultured cortical neurons [31] The neuroinhibitory action of GIF is very specific to this MT isoform and can be attributed to the small differences in the protein sequence of this isoform...

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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt

Ngày tải lên : 19/02/2014, 13:20

... conformation of backbone had an interproton error of less ˚ than 0.2 A compared to upper and lower boundaries of distances from ROE/NOE data and (b) the conformation had / angles within 30° of the ... percentage inhibition of peptide-treated cells and expressed as the mean of three independent experiments activity of 40% at 100 lM concentration Both linear and cyclic ER peptides (lER and cER) showed ... inhibition of anti-CD58 binding to CD58 expressed on the surface of MOLT-3 cells was evaluated Figure shows that the peptide lVY enhanced the binding of FITC-anti-CD58 in a concentration- dependent manner,...

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Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

Ngày tải lên : 21/02/2014, 00:20

... agonistic and antagonistic activity Eur J Biochem 267, 3032–3039 Brandenburg, K., Schromm, A.B., Koch, M.H.J & Seydel, U (1995) Conformation and ﬂuidity of endotoxins as determinants of biological activity ... MfGl-II, and LPS Re 1172, 1085, and 1038 cm)1 assigned to glucose ring vibrations [31] As no additional bands in the range of the amide vibrations centered at 1650 and 1550 cm)1 can be observed, any ... expected, activity of LPS in the range of down to 10 pgÆmL)1, whereas MfGl-II has only activity in the range ‡10 mgÆmL)1 The latter result also excludes a signiﬁcant contamination of the MfGl-II...

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Tài liệu Báo cáo khoa học: Moult cycle-related changes in biological activity of moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) in the crab, Carcinus maenas From target to transcript ppt

Ngày tải lên : 21/02/2014, 00:20

... (or both) of the above-mentioned processes may be relevant to moult control, we ﬁrst investigated the biological activity of MIH and CHH during precisely timed stages of the moult cycle of Carcinus ... binding Membrane quantities were reduced to 20–25% of those reported previously Data reduction and analysis was carried out using a radioligand binding analysis program (ElsevierBIOSOFT) Experiments ... statistically signiﬁcant) inhibition of ecdysteroid synthesis by both and 50 nM concentrations of MIH and CHH When pooled extracts of incubation media (from these experiments) were analyzed by HPLC-RIA,...

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Tài liệu Báo cáo khoa học: Solution NMR structure of ﬁve representative glycosylated polyene macrolide antibiotics with a sterol-dependent antifungal activity doc

Ngày tải lên : 21/02/2014, 03:20

... RESULTS NMR assignments of nystatin A1 and rimocidin in methanol-d4 The structure-speciﬁc assignment of the 1H and 13C resonances of nystatin A1 and rimocidin in neat methanol-d4 (Table 1) were ... and the oxygen of OH7 (OH7) which can contribute to the stabilization of the C7–C9 region of the macrolide Structural analysis of nystatin A1 The spectral degeneracy of 1H resonances in the polyol ... pimaricin, nystatin A1 and rimocidin ([29,30] and this study); a ternary mixture of methanol/pyridine/DMSO (2 : : 1) for candidin [33]; and a binary mixture of pyridine/methanol (9 : 1) for vacidin...

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Báo cáo khoa học: Casein phosphopeptide promotion of calcium uptake in HT-29 cells ) relationship between biological activity and supramolecular structure ppt

Ngày tải lên : 16/03/2014, 05:20

... extent of the measured effect is much more limited, over the whole CPP and [Ca2+]o concentration range explored Second, the highest activity, within the inves5002 tigated range of Ca2+ concentration ... individual CPPs, b-CN(1–25)4P and as1-CN(59–79)5P, (A) and (C), respectively, and of a mixture of CPPs (CPP DMV) (B) Each point on the graphs corresponds to the mean value of the [Ca2+]i peak rise ... for concomitant presence of Ca2+ and CPP for optimal aggregation) At the same time, they also demonstrated that the ability to aggregate, in terms of dimension and concentration of aggregates,...

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Báo cáo khoa học: Construction and biological activity of a full-length molecular clone of human Torque teno virus (TTV) genotype 6 pptx

Ngày tải lên : 16/03/2014, 05:20

... in human PBMC [19], bone marrow [10] and several other organs [20] However, the main host cells and the target organs of this virus are still undeﬁned The study of the biological functions of TTV ... facility of the Haartman Institute, University of Helsinki, Finland Plasmid constructs for transfection For functional analysis of the TTV clone, the cells were transfected with TTV plasmids of two ... (1991) Molecular cloning and sequence analysis of the genome of chicken anaemia agent J Gen Virol 72, 2003–2006 22 Meehan BM, Creelan JL, McNulty MS & Todd D (1997) Sequence of porcine circovirus...

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Báo cáo khoa học: Physicochemical characterization of carboxymethyl lipid A derivatives in relation to biological activity docx

Ngày tải lên : 23/03/2014, 13:20

... S, and inclination angle h between diglucosamine ring plane and membrane plane for the synthetic lipid A analogs The R values were evaluated from the ratio of the band intensities of the 90° and ... Solingen, Germany) Cell HEK293 cells were transiently transfected using the followculture supernatants and the standard [recombinant human ing protocol For transfection, 0.2 lg of the indicated ... mononuclear protocol Transient transfections were carried out in triplicells, and incubated at 37 °C cate and data given are the mean and standard deviation Antagonism Binding of monoclonal antibodies Mononuclear...

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Báo cáo Y học: Concentration-dependent reversible activation-inhibition of human butyrylcholinesterase by tetraethylammonium ion pptx

Ngày tải lên : 24/03/2014, 03:21

... Recombinant wild-type and mutant human BuChEs Two amino-acid residues (D70 and Y332) in the PAS and two (W82 and A328) in the CS, known to play a role in the binding of positively charged ligands and ... Stojan et al (Eur J Biochem 269) Ó FEBS 2002 Fig Dependence of the activity of various human BuChEs and horse BuChE on the concentration of TEA at various butyrylthiocholine concentrations BTC concentrations ... wild-type enzyme and the A328Y mutant and was still 10 times higher than the concentrations of the double mutants Experimental data in all diagrams cannot predict the extent of inhibition at...

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Báo cáo Y học: Synthesis, conformational analysis and biological activity of cyclic analogs of the octadecaneuropeptide ODN Design of a potent endozepine antagonist pot

Ngày tải lên : 24/03/2014, 04:21

... response with that of ODN and OP revealed that, at a concentration of 1028 M , cyclo128 OP was more efﬁcient than ODN and OP (Fig 3C) Administration of graded concentrations of OP and cyclo128 OP ... amide proton exchange rate, using only the angle and distance restraints deduced from 3JNH-Na coupling constant and NOE cross peak, respectively Analysis of the (F, C) angles, and the potential ... the analysis of the calculated structures revealed a short distances between NH of Leu7 and the side chain carboxyl group of the Asp6 residue (< 0.28 nm), and NH of Leu7 residue and C¼O of Leu5...

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Báo cáo hóa học: " In vivo evidence of htid suppressive activity on ErbB-2 in breast cancers over expressing the receptor" potx

Ngày tải lên : 18/06/2014, 16:20

... Society (grant 10-1695Ku2 to UK-D) and by Associazione Italiana Ricerca sul Cancro (AIRC) (grant to PGN) We would like to thank Prof Forni, Dept of Clinical and Biological Sciences, University of Turin, ... regulator of ErbB-2 activity The inverse correlation of the expression levels of the two genes is highly significant in human breast and nonmammary cancers, and in experimental tumors raised in transgenic ... inverse correlation of the expression profiles of the two proteins in human breast tumors and non mammary malignancies provides an in vivo proof for the oncosuppressive activity of htid on ErbB-2...

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báo cáo hóa học:" Passage and concentration-dependent effects of Indomethacin on tendon derived cells" pot

Ngày tải lên : 20/06/2014, 01:20

... absorbances of Alamar blue and methylene Comparison of absorbances of Alamar blue and methylene blue obtained after culture of rat tail tendon derived cells at varying concentrations for and days ... conception and design, or acquisition of data, or analysis and interpretation of data and have been involved in drafting the manuscript or revising it critically for important intellectual content; and ... Trauma Surg 1987, 106(3):157-60 Wang ED: Tendon repair Journal of Hand Therapy 1998, 11:105-10 Jozsa L, Kannus P: Human Tendons: Anatomy, Pyhsioloogy and Pathology Human Kinetics Europe Ltd; 1997...

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Báo cáo toán học: "Role of One N-linked Oligosaccharide Chain on Canine Herpesvirus gD in Its Biological Activity" ppt

Ngày tải lên : 07/08/2014, 11:21

... precursor form of the CHV gD These bands were smaller than those (approximately 51–55 and 48 kDa) of pME-cgD (YP2)transfected COS-7 cells In pME-fgD-transfected cells, any specific band was not ... Immunoblot analysis of gDs expressed in COS-7 cells with a mixture of MAbs against CHV gD Lane 1; pME-cgD (YP2)-transfected COS-7 cells, lane 2; pME-cgD (YP11mu)-transfected COS-7 cells, and lane 3; ... 3; pME-fgD-transfected COS-7 cells (B) Immunoblot analysis of gDs expressed in Sf9 cells with an MAb 10C10 against CHV gD Lanes and 3; AccgD (YP2)-infected Sf9 cells, and lanes and 4; AccgD (YP11mu)-infected...

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Báo cáo vật lý: "Resveratrol Derivatives from Stem Bark of Hopea and Their Biological Activity Test" docx

Ngày tải lên : 07/08/2014, 14:20

... radical and form water molecules, whereas radical phenol will be stabilized by resonance That is, resveratrol compound is referred for development as antioxidant An antioxidant is substance that can ... isolated from the stem bark of Hopea consist of dimer, trimer and tetramer resveratrol Some compounds have biological activity as antioxidant and cytotoxic effect against Raji and HeLa-S3 cell lines ... Carita, Pandeglang, Banten, Indonesia and Herbarium Bogoriensis for the sample gift and the identification of the plant specimen REFERENCES Cronquist , A (1981) An integrated system of classification...

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Báo cáo y học: "Physical function, disease activity, and health-related quality-of-life outcomes after 3 years of adalimumab treatment in patients with ankylosing spondylitis" potx

Ngày tải lên : 09/08/2014, 14:22

... BASDAI n Mean ± SD change BASFI n Mean ± SD change SF-36 PCS n Mean ± SD change SF-36 MCS n Mean ± SD change SF-36 Physical Function n Mean ± SD change SF-36 Role–Physical n Mean ± SD change SF-36 ... Mean ± SD change SF-36 Vitality n Mean ± SD change SF-36 Social Function n Mean ± SD change SF-36 Role–Emotional n Mean ± SD change SF-36 Mental Health n Mean ± SD change ASQOL n Mean ± SD change ... We thank Dana L Randall, MS, PharmD, of Arbor Communications, Inc., Ann Arbor, Michigan, and Michael A Nissen, ELS, of Abbott Laboratories, for assistance with manuscript editing, revision, and...

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báo cáo khoa học: " Biological activity of a genetically modified BMP-2 variant with inhibitory activity" potx

Ngày tải lên : 11/08/2014, 20:20

... doses of 10, 40 and 160 μg Thus the number of animals was n = 18 After a period of 28 days the animals were sacrificed and the specimens were explanted Examination of the implants After explantation ... Opperman LA: Transforming growth factor-beta isoform expression in the perisutural tissues of craniosynostotic rabbits Cleft Palate Craniofac J 2004, 41:392-402 Glaser DL, Economides AN, Wang L, ... alterated effects for mutants of epitope and epitope as well But only alterations of epitope lead to a more or less strong inhibition of the activity of BMP-2 wild type Necessary concentrations for half-maximal...

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