2ovarian germ cell tumours

... specifically expressed in germ cells of both male and female oysters, and we hypothesized that Oyvlg had a role in germ cell formation [17] However, the function of Oyvlg in germline development had ... reproductive deficiency The premeiotic male germ cells ceased their differentiation before the pachytene In vivo injection of oyvl-dsRNA provokes abnormal germ cell development One month postinjection, ... defects in germ cell development affecting all of the gonadic area, in both females and males Upon histological examination of gonads injected with 20 lg of dsRNA, there were fewer germ cells, and...

Ngày tải lên: 07/03/2014, 00:20

... vitro cell- free methods for protein synthesis with extracts from prokaryotic [1] or eukaryotic [2] cells Wheat germ cell- free eukaryotic protein production offer an alternative to the E coli cell- based ... wheat germ cell- free method comparable to that of the E coli cell- based approach for NMR structure determinations of eukaryotic proteins One of the main advantages of the automated wheat germ cell- free ... normal multinuclear NMR spectra Cell- free protein production protocols are streamlined compared to cell- based protocols, in that they not require cell harvesting or cell lysis Protein purification...

Ngày tải lên: 07/03/2014, 12:20

... testicular Leydig cells in vitro [11] 874 Germ cells constitute the major inner seminiferous tubular cell mass and the role of H2O2 as a signalling molecule in apoptotic induction among germ cells is ... penicillin The cell viability of the final crude population that contained mostly maturing germ cells was in excess of 95% H2O2 treatment The isolated testicular germ cells (5 · 106 cellsÆmL)1 per ... Involvement of Bcl-2 family proteins in germ cell apoptosis during testicular development in the rat and pro-survival effect of stem cell factor on germ cells in vitro Mol Cell Endocrinol 165, 115–129 25...

Ngày tải lên: 16/03/2014, 04:20

... Nonseminomatous Germ Cell Tumor of the Testis 23 Paul H Johnston and Stephen D.W Beck Chapter Diagnostic Imaging of Intracranial Germ Cell Tumors: A Review 47 Takamitsu Fujimaki Chapter Testicular Germ Cell ... Epigenetic Modifications in Testicular Germ Cell Tumors 107 Christopher J Payne Chapter Claudins and Germ Cell Tumors Ylermi Soini 135 Preface In Germ Cell Tumor, leading scientists and physicians ... options for the most common type of germ cell tumors of the testes, non-seminomatous germ cell tumors In Chapter 3, Takamitsu Fujimaki reviews intracranial germ cell tumors, which affect mostly...

Ngày tải lên: 17/03/2014, 00:20

... protein N-myristoylation in plant cells in more detail, we established a cell- free protein N-myristoylation system as an application of an advanced wheat germ cell- free translation system that ... Myr–GFP synthesized in the wheat germ cell- free translation system Effect of the combination of amino acids at positions and on protein N-myristoylation in the wheat germ cell- free translation system ... and of the consensus motif in plant target proteins with the use of a Cell- free N-myristoylation of plant proteins wheat germ cell- free translation system We identified several new N-myristoylated...

Ngày tải lên: 29/03/2014, 21:20

... Features Assay type Cell types Cells enter or cross cellulose membrane Cells move toward and enter capillary filled with attractant Cells avoid capillary filled with control buffer Cells migrate in ... and direction changes of individual cells (together referred to as “tracking” assays) as well as determinations of cell orientation, cell cell interactions, cell morphology, flagellar wave patterns, ... reservoirs; cells adhere to cover glass during migration Cells migrate between cover glass and slide; cells and chemoattractant released from wells Cells migrate across a circular bridge separating cell...

Ngày tải lên: 11/04/2014, 09:43

... midgestation of putative germ cell- derived clones, we hypothesize that either gonadal cells other than germ cells may have been picked instead of true germ cells or that a subset of germ cells may not have ... developmental potential of germ cell nuclei after transfer into oocytes remains controversial To date, gonadal cells bearing morphological resemblance to germ cells have been used or germ cells have been ... donor cells, either ES, EC, or germ cells could be used In this study, germ cells are used as they match cumulus cells for direct recovery from the body and absence of in vitro culture Gonadal cells,...

Ngày tải lên: 11/04/2014, 09:43

... 4.0% for renal cell carcinoma, 0.84% to 3.6% for transitional cell carcinoma, 0.4% for germ cell tumors, and 0.36% to 0.7% for prostate adenocarcinoma[9] Testicular mixed germ cell tumors are ... testicular mixed germ- cell tumors is because germ cells in the testes are totipotent and can undergo either trophoblast or somatic differentiation Among all the subtypes of testicular germ cell tumor, ... Cutan Pathol 2009, 37(4):486-90 16 International Germ Cell Consensus Classification: A prognostic factor based staging system for metastatic germ cell cancers J Clin Oncol 1997, 15:594-603 doi:10.1186/1477-7819-8-21...

Ngày tải lên: 09/08/2014, 03:21

... (Figure 1c) The diagnosis was of an International Germ Cell Cancer Collaborative Group (IGCCCG) poor prognosis metastatic nonseminomatous germ cell tumor Four cycles of bleomycin, etoposide and ... European Consensus Conference on Diagnosis and Treatment of Germ Cell Cancer: A report of the Second Meeting of the European Germ Cell cancer Consensus group (EGCCCG): Part Eur Urol 2008, 53:478-496 ... indicates a poor prognosis within the IGCCCG classification [3] The spread of nonseminomatous germ cell tumors may be local, via the lymphatics or via vascular channels Review of our patient’s...

Ngày tải lên: 11/08/2014, 03:20

... Matsuda H: Primary germ cell tumors in the mediastinum: a 50-year experience at a single Japanese institution Cancer 2003, 97(2):367-376 Droz JP, Horwich A: Extragonadal germ cell tumors Comprehensive ... patient’s pituitary showing polymorphic cells with granular cytoplasm Discussion This case study is unusual because our patient had both SLE and pituitary germ cell tumor It is also unusual because ... Rheum 2003, 48:2100 doi:10.1186/1752-1947-4-97 Cite this article as: Shahrir et al.: Extragonadal germ cell tumor presenting in a woman with systemic lupus erythematosus: a case report Journal of...

Ngày tải lên: 11/08/2014, 12:20

... skin tumours (Pct of all benign tumours) Basal cell tumour 12.0% (20.7%) Perianal gl adenomas 8.3% (19.8%) Histiocytoma 7.8% (18.6%) Histiocytoma 14.0% (29.0%) Lipoma 6.0% (12.4%) Basal cell ... 6.1% (11.2%) MCT 10.0 (45.4%) STS 4.3% (19.6%) Malignant epidermal tumours 3.4% (15.4%) MCT: Mast cell tumours, SCC: Squamous cell carcinoma, STS: Soft tissue sarcoma SCC 16.4% (35.9%) Melanoma ... (Pct of all benign tumours) Top malignant Pct of total skin (pct of all malignant skin tumours) USA [3] #, N = 277 B: 54.4% M: 100% M: 45.6% Epidermal adenoma 22.7% Basal cell tumour 18.8% (34.6%)...

Ngày tải lên: 12/08/2014, 18:22

... protein that promotes cell survival and has a role in maintenance of reproductive germ cells The knockout leads to progressiveloss of germ cells, Sertoli cells and Leydig cells and cause extensive ... deriving germ cells from human ES cells will permit studies of germ cell differentiation (Surani 2004) Not only will these approaches be used to identify the signals involved in regulating germ cell ... mediating cell cycle regulators, growth factors and intracellular communication pathways for germ cell development Cytoplasmic and Apoptotic Factors During gametogenesis, apoptosis of germ cells...

Ngày tải lên: 25/10/2013, 21:20

... purified by gel filtration with NICK columns and finally by ethanol precipitation Cell- free translation The wheat -germ extract was prepared by the reported method [1] DTT in the extract (4 mM) was ... the wheat -germ extract at a high concentration for the sake of stability during storage [1] Thus, in order to reduce the DTT concentration for the translation reaction, Ó FEBS 2003 Cell- free ... the crude cell- free protein synthesis solution The present biotinylation technique may be useful for preparation of biotin-labeled proteins and also for nonradioisotope detection of cell- free...

Ngày tải lên: 16/03/2014, 23:20

... fibrocarcinoma cells and B16 melanoma cells [49] Moreover, Dong & Wang demonstrated the possibility of death-resistant cell selection by the repetition of hypoxia episodes [61] Such selected cells were ... some solid tumours [90– 92] One of the main targets of these treatments comprises the endothelial cells One endothelial cell can control the survival of approximately 50–100 tumour cells [93] ... endothelial cells may induce the death of a large number of tumour cells Moreover, it was shown that endothelial cell suppression could also mediate apoptosis in drugresistant tumour cells [94,95]...

Ngày tải lên: 30/03/2014, 04:20

... brief, cells were seeded in 96 well plates at a cell line specific density to ensure exponential growth throughout the whole period of the assay These cell numbers were determined previously by cell ... FWGE Page of Pasching, Austria FBS was purchased Biochrom AG, Berlin, Germany Cell lines and culture The following human cancer cell lines were used for experimentation: testicular cancer (H12.1, ... death induced by FWGE in a panel of cell lines In order to distinguish the mode of cell death induced by FWGE we treated a representative panel of human cancer cell lines with an IC90 of FWGE for...

Ngày tải lên: 10/08/2014, 10:21

... sites In 1999, Bell et al described CHK2 germ line mutations in patients suffering from Li-Fraumeni syndrome or Li-Fraumeni-like syndrome [11] without a germ line p53 mutation One of the most important ... carcinoma, a basal cell carcinoma, and a melanoma; another one suffered from a prostate and a bladder carcinoma, and one patient experienced a colon carcinoma and a basal cell carcinoma Table ... from multiple tumor types including squamous cell carcinoma of the head and neck, showed no CHK2 1100delC variant This indicates that this particular germ line variation plays no significant role...

Ngày tải lên: 11/08/2014, 07:21

... bào gốc thai, tế bào gốc trưởng thành a/ Tế bào gốc phôi (Embryonic stem cells-ESCs) tế bào mầm phôi (Embryonic germ cells) Tế bào gốc phôi tế bào gốc vạn lấy từ phôi giai đoạn sớm (4-7 ngày ... năng: d/ Tế bào gốc đơn (mono/unipotential progenitor cells): Tế bào gốc đơn năng, gọi tế bào định hướng đơn dòng hay tế bào đầu dòng (progenitor cells), tế bào gốc có khả biệt hóa theo dòng Ví dụ ... tế bào, mô định Các tế bào gốc phôi lấy từ khối tế bào bên (inner cell mass) tế bào gốc vạn c/ Tế bào gốc đa (multipotent stem cells): Là tế bào có khả biệt hóa thành nhiều loại tế bào thể từ...

Ngày tải lên: 18/08/2012, 20:56

... of cell growth and proliferation The book concludes with a chapter by Rajesh and Pittman, who discuss the relations of cell cycle regulators and mammalian germ cells The future challenges in cell ... simple unicellular bacteria to complex multicellular animals, revealed that this process involves duplicating cellular contents followed by division into two identical cells (Nurse 2000a) Cell cycle ... uncovered in p27Kip1 -deficient cells Curr Biol 9:163–173 Conlon I, Raff M (2003) Differences in the way a mammalian cell and yeast cells coordinate cell growth and cell- cycle progression J Biol...

Ngày tải lên: 07/10/2012, 16:51

... uniform adipose cells Spindle cell lipomas contain collagen fibers and small, cylindiric spindle cells and mixed mature adipocytes within a matrix containing mucinous material Spindle cell lipomas ... spindle cells are dominant Spindle cells appear as uniform cells with single elongated nucleus, and narrow bipolar cytoplasm in SCL Nucleolus is not prominent Mitosis is rare Spindle cells form ... with spindle and star-shaped cells lacking well-defined borders located beneath the squamous epithelium in the myxoid area No mitosis was observed, while rare giant cells were detected (Figure...

Ngày tải lên: 25/10/2012, 10:51

... studies, NDP was found to be highly effective against solid tumors, including non-small cell lung cancer, small cell lung cancer, head and neck cancer and esophageal cancer [15] The replacement of ... squamous cell carcinoma of the esophagus J Clin Oncol 1999; 17: 2915-21 Kaneko K, Ito H, Konishi K, et al Definitive chemoradiotherapy for patients with malignant stricture due to T3 or T4 squamous cell ... Demographic and Clinicopathologic Characteristics of 56 Japanese Patients with Esophageal Squamous Cell Carcinoma Characteristics Values Age, yr 64.3±7.5 (48 -78) Height, cm 163.1±6.7 (150-180) Weight,...

Ngày tải lên: 26/10/2012, 09:53