2 scaffolds for vascular tissue engineering

... pore size Small pores induce osteochondral formation before osteogenesis, whilst large pores that are well vascularized induce direct osteogenesis Tissue forms quickly on the outer surfaces of the ... thanks goes to Chris Lam for his guidance to develop my laboratory skills, Anand for his true support during the screw extrusion system development and maintenance, and Andrew for his valuable suggestions ... dispensing speed for 0.5 mm nozzle diameter 48 Figure 3.15 Theoretical and experimental porosity vs dispensing speed for different nozzle diameter 49 Figure 3.16 Modulus vs dispensing speed for different...

Ngày tải lên: 10/09/2015, 15:51

... (stereolithography) format into QS (QuickSlice), slicing of the CAD model into horizontal layers and conversion into SLC format, creation of a deposition path for each layer and conversion into SML format for ... engineering for bone regeneration is the scaffold that serves as a 3D template for initial cell interactions and the formation of boneextracellular matrix to provide structural support to the newly formed ... process He has been an immense driving force behind this project One simply could not wish for a better or friendlier supervisor  Dr Bina Rai, mentor, for graciously sharing her knowledge and...

Ngày tải lên: 09/10/2015, 11:18

... Ong Lee Lee and Chia Ser Min for their expertise in performing cell immunofluorescence staining; Drs Ying Lei and Cen Lian from the Department of Chemical Engineering for their guidance on bio-conjugation; ... material selection for liver tissue engineering 2-11 Scaffold fabrication for liver tissue engineering 2-16 Scaffold surface modification 2-20 Tissue culture for liver tissue ... role in contrast to vascular endothelial cells They perform special functions like filtration, regulation of sinusoidal blood flow and endocytosis [3] Kupffer cells account for about 10% of total...

Ngày tải lên: 12/09/2015, 10:22

... medium to secrete ECM and form cell sheets, and the cell sheets were then wrapped around a temporary inert tubular support and allowed for maturation for some time to form a cylinder composed ... achieve improved systematic performance for the resultant construct Composite vascular graft constructions have been attempted in different approaches Acellular composite vascular grafts have been ... requirement for the structural or chemical properties of the components Therefore, choices of appropriate materials and techniques with fewer limitations are crucial for the development of composite vascular...

Ngày tải lên: 10/09/2015, 09:08

... numbers, 3000 cells, by centrifugation to form EBs In doing so, a reproducible and robust platform based on hESC differentiation through EB formation was set up for analysis of growth factor influence ... addition, biomaterials may also provide physical cues for cell orientation and spreading, which are critical for hESC differentiation and tissue formation For example, co-cultured hESC-derived chondrogenic ... functional cell source for cartilage repair Chondrocytes, fibroblasts, stem cells, and genetically modified cells have all been explored for their potential as viable cell sources for cartilage tissue...

Ngày tải lên: 11/09/2015, 09:57

... as temporary scaffolds for cell adhesion, guiding tissue formation while performing biochemical and biomechanical functions before the regenerative process is complete For example, bioresorbable ... efficient tool for obtaining high yield mesenchymal stem cells (MSCs) isolation and expansion in-vitro • Establish the apatite-based porous microcarriers as an efficient platform for driving directed ... responsible for its unique rigidity, viscoelasticity and toughness In addition to contributing to its mechanical properties, the microstructure of bone forms a unique microenvironment for the bone...

Ngày tải lên: 22/09/2015, 15:18

... bioreactor design I very much appreciate the efforts of Ms Julee Chan, senior lab officer in histology, for providing valuable information while performing histological analysis of the samples ... in a bioreactor ex vivo (in vitro) for a period of time before transplantation Special reactors are required to conduct biochemical reactions for the transformation and production of chemical ... National University of Singapore for their guidance and support during this research First and foremost, I would like to thank my advisor, Assoc Prof James Goh for providing me an opportunity...

Ngày tải lên: 04/10/2015, 10:25

... properties for appropriate applications Natural, ECM-derived proteins are apparently the first choice for soft TE, since they provide a physiologically relevant and recognizable platform for the ... with PBS, fixed using formalin for 20 minutes at RT and permeated using 0.1% Triton-X100 for minutes at RT Non-specific sites is blocked using 3% bovine serum albumin (BSA) for 90 minutes period ... the biomaterial aids in vivo revascularization as well as integration with the host tissue There are additional specific requirements for a biomaterial to be applied for CTE It includes that the...

Ngày tải lên: 05/10/2015, 19:04

... like to thank her for reviewing the drafts  Dr Simon Cool (IMB), project mentor, for his suggestions given on the progress of this project during presentations  Richard Lin (3M), for his assistance ... Instron micro tester for mechanical testing  Ms Irene Kee (Dept of Experimental Surgery, SGH), for her valuable assistance and support during animal surgeries and taking care for them after implantations ... current treatments for bone defects For surgical procedures involving bone grafts, patients usually suffer from trauma-related injuries or bone fractures Currently, the gold standard for bone grafts...

Ngày tải lên: 09/10/2015, 11:18

... cost of life 1.5 Medical Options for Heart Disease 1.5.1 Drugs for Heart Disease The conventional treatment for cardiovascular disease includes specific medication for any underlying causes Various ... for cardiac tissue replacement, further confirming its huge promise for cardiovascular tissue engineering application This thesis reports on a novel hybrid nanofibrous PCL membrane scaffold for ... absorbable biomaterials, to guide the formation of new cardiovascular tissues Initial targets of the research program will include heart valves, vascular grafts, and cardiovascular patches Successful development...

Ngày tải lên: 26/11/2015, 22:40

... regeneration site, and a facility for promoting capillary formation, in other words, neovascularization When a permissive environment optimal for cells to regenerate tissues is formed expectedly by these ... attachment, the barrier for cells to retain the site for tissue regeneration, the guide for cells to create a tissue giving the contour of the regenerated tissue, the carrier for the sustained release ... provide an attachment site for the cells responsible to the tissue regeneration Similar to embryonic development, multiple cells should assemble to a specified form for tissue formation To this end,...

Ngày tải lên: 25/10/2013, 08:20

... pulpal involvement) The lack of any enamel forming cells in the enamel of fully developed erupted teeth precludes the potential for cell-based approaches for enamel regeneration In contrast, the ... intimate contact with an underlying highly vascular and innervated pulpal tissue, forming a tightlyregulated "dentin-pulp complex" During primary tooth formation, dentin is produced by odontoblastic ... Conventional techniques for inducing pulpal repair Calcium hydroxide has long been the "gold standard" for pulp capping [16] Its effectiveness at promoting dentinal bridge formation over small pulpal...

Ngày tải lên: 11/08/2014, 23:22

... (GPBE), for their great effort in making GPBE an enriched, prosperous, and warm “family” My special appreciation to my friends in GPBE, especially the 2003 batch students, for all the unforgettable ... veins/arteries for grafting due to preexisting vascular diseases, vein stripping, or vein uses in prior procedures Nowadays more than 600,000 vascular grafts bypass procedures are performed annually ... challenge that vascular tissue engineering is seeking to answer One of the commonly accepted methods for developing tissue engineered small-diameter vascular grafts is to hybridize vascular grafts...

Ngày tải lên: 11/09/2015, 16:07

... Chapter Isolation and Characterisation Of Vascular Progenitors Figure 4-3: Primary culture of haemopoietic tissue in EGM Following culture ... cells, and failed to express endothelial phenotype 122 Chapter Isolation and Characterisation Of Vascular Progenitors (a) (b) (c) (d) (e) (f) Figure 4-4: CFU assay Adherent colonies appeared after...

Ngày tải lên: 14/09/2015, 08:49

... filament proteins, for example, cytokeratins for epithelial cells, vimentin for mesodermal cells such as fibroblasts, endothelium, and myoblasts, desmin for myocytes, neurofilament protein for neuronal ... discussions with our colleagues We made every effort to provide a user-friendly reference for sourcing, characterization, and use of cells for tissue engineering, for researchers with a variety of backgrounds ... publishes its books in a variety of electronic formats Some content that appears in print may not be available in electronic formats For more information about Wiley products, visit our web site...

Ngày tải lên: 22/03/2014, 15:20

... mixture of cell suspension and fibrinogen for direct transplantation to the injured spinal cord is another approach for application of fibrin in clotted form But, such a preparation made of olfactory ... cavity surrounded by an astrocytic scar is formed following this tissue loss Where the injury extends to pia mater, collagen will also contribute in the formation of the scar tissue As Approaches ... et al [27] and Baptiste and Fehlings [28] for review), neutralization of the effects of regenerative sprouting inhibitory proteins (see Scott et al [29] for review) and gene therapy (see Blits...

Ngày tải lên: 19/06/2014, 10:20

... devices for reconstruction and repair of the organ structures in order to maintain and improve their function [1] The production of scaffolds, which are used for framework and initial support for ... satisfactory for hard tissue applications Hence, the purpose of the present study was to create a 3D scaffold with enhanced mechanical and biological properties through multi-pore formation and ... cellulose, 5% ammonium polyacrylate dispersant, and 5% N,N-dimethylformamide drying agent) were added to the slurry to improve the sintering force and to stabilize the scaffold structure The polyurethane...

Ngày tải lên: 21/06/2014, 17:20

... is advantageous, for it allows for the thorough characterization of the genetically engineered cells prior to implantation, possibly for the preservation of some of the cells for later use by ... address this problem, methods have been developed for islet encapsulation in thin conformal polymeric coats Materials that have been used for conformal coating include photopolymerized poly(ethylene ... therefore exists in enhancing immune reconstitution through regenerative or cell therapies for boosting thymus activity in vivo or for providing customized in vitro–generated T-cell repertoires for...

Ngày tải lên: 29/06/2014, 09:21

... development – for example, at what times of year the vascular cambium is active, Thresholds for secondary vascular development in which months phloem is being produced, and so on – conforms to the ... to eight Thresholds for secondary vascular development a 569 vicinity This would account for the staggered arrangement of the groups between neighbouring tangential rows and for the interpolation ... course of an iterated search for the best fit between the accumulated cell number and thermal time Thresholds for secondary vascular development 567 2.3 More complex units for development − developmental-time...

Ngày tải lên: 08/08/2014, 00:22