2metabolism of polycyclic aromatic hydrocarbons by fungi and yeasts

... (Valli and Gold 1991) and by a strain of Acinetobacter sp (Beadle and Smith 1982); • The degradation of quinoline by pseudomonads and by Rhodococcus sp (Schwarz et al 1989); • The degradation of ... and Smith 1982) followed by ring cleavage are available (Section 6.5.1.2.) 6.2.2 Metabolism of Polycyclic Aromatic Hydrocarbons by Fungi and Yeasts Fungal metabolism of PAHs has been studied ... metabolism of ethene by Mycobacterium sp strain E44 (Wiegant and de Bont 1980), of styrene by a strain of Xanthobacter sp strain 124X (Hartmans et al 1989), and by Corynebacterium sp strain ST-5 and...

Ngày tải lên: 11/08/2014, 04:20

... scholarship and the financial support -i- PhD Thesis Jiang Zhaoqin Table of Contents Acknowledgements i Table of Contents ii Summary ix List of Tables xi List of Schemes xiii List of Figures xv List of ... Results of non-linear effect study could lead to a better understanding of the role played by the different ee of organocatalysts for aldol reaction and could also enhance our understanding on ... 4-8a-b and 4-9a-c 142 Scheme 4-4 Synthesis of 2-hydroxy-γ-butyrolactones 150 Scheme 5-1 Structures of varacin, varacin A, varacin B and varacin C 193 Scheme 5-2 Proposed mechanism of DNA cleavage by...

Ngày tải lên: 14/09/2015, 08:39

... downregulation of mRNA and protein levels of PAR-1 On the other hand, inhibition of PKA by its inhibitor H-89 prevented PAR-1 downregulation Similarly, the involvement of the PKA pathway and the lack of ... action of PGE2 and ligands of receptor A2B and b2-AR (NECA and ISO) might result from different effects of those compounds on PAR-1 mRNA stability However, PGE2 did not influence the rate of PAR-1 ... inverse correlation of expression levels of AP-2 and PAR-1 was shown [19] We tested the involvement of Sp1 and AP-2 transcription factors in PAR-1 expression and the possible influence of PGE2 on their...

Ngày tải lên: 30/03/2014, 04:20

... expression by TNFa via the activation of NF-jB Results Upregulation of PAI-1 expression in endothelial cells by ROS In preliminary experiments, we evaluated the role of ROS during the stimulation of ... determined by ELISA Figure 1A shows that the treatment of endothelial cells with TNFa increased PAI-1 antigen by almost twofold (180 ± 10%, P < 0.05) Cotreat5822 A B Fig Effect of TNFa and H2O2 ... plasmid and treated with TNFa in the presence or absence of NAC NF-jB promoter activation by TNFa was inhibited by antioxidant, NAC (Fig 3D) To confirm the role of fragment ()664 to )680) of PAI-1...

Ngày tải lên: 30/03/2014, 11:20

... Page of 11 Figure Effect of PEP-1-SOD1 and PEP-1-CAT on Bcl-2 and Bax expression Bcl-2 and Bax expression was detected by Western blot as described in Materials and Methods (A), and quantified by ... followed by h of reoxygenation Apoptosis was measured by staining the cells with Annexin V and PI followed by Flow cytometry The apoptosis rates are shown Figure Effects of PEP-1-SOD1 and PEP-1-CAT ... t-test and one-way analysis of variance followed by a Newman-Keuls post hoc test Probability values of P < 0.05 were considered to be significant Result Expression and purification of PEP-1-SOD1 and...

Ngày tải lên: 18/06/2014, 19:20

... presence of the ligands to these chemokine receptors RANTES, MIP-1α and MIP-1β, all of which are ligands for CCR5, inhibit macrophage-tropic isolates, whereas SDF-1, the specific ligand for CXCR4, ... concentrations of various drugs required to inhibit and to partially inhibit the fusion of TF228.1.16 and 293/CD4+ (Table 1) All these drugs decreased the percentage of surface covered by syncytia ... I (0.20) I (3.52) §F = 50–60% of the surface is covered by syncytia; P = partial inhibition of fusion: < 10% of the surface is covered by syncytia; I = inhibition of syncytia formation TF228.1.16...

Ngày tải lên: 18/06/2014, 22:20

... Effects of IL-6 and GM-CSF on phosphorylation of p44/42 MAPK, p38, and JNK To determine whether IL-6 and GM-CSF could activate MAPK pathways in BMECs as in the case of LPS phosphorylation of MAPKs ... Effects of antibodies to IL-6 and GM-CSF on LPS-induced changes in the permeability of BMECs to 131I-HIV-1 (A and C) and TEER (B and D) LPS (100 µg/mL) was added to the luminal chamber In panels A and ... ZO-1, and actin were detected by western blot Relative intensity of occludin (A), claudin-5 (B), and ZO-1 (C) is calculated as ratio of arbitrary densitometric units of target protein to that of...

Ngày tải lên: 19/06/2014, 22:20

... presence of the ligands to these chemokine receptors RANTES, MIP-1α and MIP-1β, all of which are ligands for CCR5, inhibit macrophage-tropic isolates, whereas SDF-1, the specific ligand for CXCR4, ... concentrations of various drugs required to inhibit and to partially inhibit the fusion of TF228.1.16 and 293/CD4+ (Table 1) All these drugs decreased the percentage of surface covered by syncytia ... I (0.20) I (3.52) §F = 50–60% of the surface is covered by syncytia; P = partial inhibition of fusion: < 10% of the surface is covered by syncytia; I = inhibition of syncytia formation TF228.1.16...

Ngày tải lên: 20/06/2014, 04:20

... to µM of fusion protein for hours, and the levels of protein transduced into the cells were analyzed by western blotting of protein extracted from the explants Faint but definite bands of trans- ... variety of cell systems and include inhibition of apoptosis by transduction of anti-apoptotic Bcl-XL protein in explant cultured human chondrocytes and in human islet cells [22,23], induction of chemosensitivity ... GTA C; and GAPDH antisense, CGT TCA GCT CAG GGA TGA CC Reactions were processed through 25 cycles of 30 seconds of denaturation at 94°C, minute of annealing at 55°C for GAPDH and 30 cycles of 45...

Ngày tải lên: 09/08/2014, 08:22

... Thu Tram and Nguyen Van Ngai for follow-up of the exposed uninfected individuals, Annie David for technical assistance, Nathaniel Landau and Jay Levy for the gift of plasmids and vectors, and Ioannis ... infection by the HIV-BaL pseudotype (white bars; n = 3, mean ± SD) of CD4 T cells from the EUs B184, W336, B195 and W278, and percentage of cells with detectable surface expression of the CCR5 ... (r2 were 0.845 and 0.826 for B195 and control, respectively) Statistical analysis and curve-fitting were performed with Sigmaplot software (Systat Software, Inc, CA, USA) Page of 12 (page number...

Ngày tải lên: 13/08/2014, 09:20

... Structure of 65 3.2.2 1H and 31P NMR of complexes 67 3.2.3 Electrochemistry of 69 3.2.4 Electronic absorption spectrum of 3+ 74 3.2.5 Structures of and 77 3.2.6 1H and 31P NMR of complexes and 81 ... Pt and Pd Centers and Acetylene and Vinylidene iv 4.1 Introduction 103 4.2 Objectives 104 4.3 Results and Discussion 108 4.3.1 Syntheses and Structures 108 4.3.2 1H and 31P NMR of complexes , and ... Electrochemistry of Polynuclear Cu(I) and Ag(I) Complexes Bearing Ferrocenyl Groups 2.1 Introduction 31 2.2 Results and Discussion 33 2.2.1 Syntheses and Structures 33 2.2.2 1H and 31P NMR of complexes and...

Ngày tải lên: 17/09/2015, 17:17

... chúng t ôi) Updat ing Sửa sonvalentine : 01-06-2011 lúc 02:45 PM hemattroi, lionmonkey, Akai90 and 77 others like this Canon EOS 1Ds Mark III,Lens:Canon EF 70- 200mm/2.8L IS II, Canon EF 85mm ... (sonvalentine - webthehinh.com) Sửa sonvalentine : 04-06-2011 lúc 08:59 AM hemattroi, y06a_ktanh, whitegun and 34 others like this Canon EOS 1Ds Mark III,Lens:Canon EF 70- 200mm/2.8L IS II, Canon EF 85mm ... CỦA TÔI HỮU ÍCH CHO BẠN Sửa sonvalentine : 01-06-2011 lúc 05:04 PM hemattroi, mrdang89, ikariam and 69 others like this Canon EOS 1Ds Mark III,Lens:Canon EF 70- 200mm/2.8L IS II, Canon EF 85mm...

Ngày tải lên: 27/08/2012, 09:21

... :) Products Microsoft OLE DB Provider for ODBC Drivers error '80004005' [Microsoft][ODBC Microsoft Access Driver] The number of columns in the two selected tables or queries of a union query not ... http://www.nelcoproducts.com/shopping/shopdisplayproducts.asp?id=153%2 0and% 201=convert(int,(select%20top% 201%20table_name%20from%20information_schema.tables)) sp_password Microsoft OLE DB Provider for ODBC Drivers error '80040e07' [Microsoft][ODBC SQL ... Products Microsoft OLE DB Provider for ODBC Drivers error '80040e07' [Microsoft][ODBC SQL Server Driver][SQL Server]Syntax error converting the nvarchar value 'AdminUsers' to a column of data type...

Ngày tải lên: 09/10/2013, 12:20

... supported by grants 30821065 and 30870559 from the China National Nature Sciences Foundation, 2006CB910703 from the Ministry of Sciences and Technology of China, and 07dz05907 from the Committee of ... h, and apoptosis was measured by AO ⁄ EB staining (D) Expression of Bcl-2 and Akt-1 in Bcl-2overexpressing 3T3-L1 adipocytes The treatment was the same as in (C) Akt-1 and Bcl-2 were detected by ... apoptosis by using 3T3-L1 adipocytes and rat primary adipocytes Adipocyte apoptosis could be induced by troglitazone, pioglitazone or 15-deoxy-D12,14-prostaglandin J2 (PGJ2), and reversed by GW9662,...

Ngày tải lên: 16/02/2014, 09:20

... increased by LP and LPS Thus, these results demonstrate that both BMDM and J774 cells express RGS1 and RGS2 mRNA and modulate expression after stimulation with LP and LPS Expression patterns of RGS1 and ... LPS and FSL-1 Involvement of TRIF in the upregulation of RGS1 and RGS2 mRNA The findings obtained from activation of TLR3 by stimulation with poly(I:C) indicate a different modulation of RGS1 and ... with LPS and LP LP and LPS mediate immunomodulating functions, at least in part, through regulation of RGS1 and RGS2 expression Results RGS1 and RGS2 mRNA expression is regulated by LP and LPS...

Ngày tải lên: 18/02/2014, 13:20

... extracts, and found that the cellular phosphorylation of CDK4 on Y17 is mediated by Src family kinases Results Detection of CDK4 Y17 phosphorylation and kinase activity The N-termini of CDKs 1, 2, and ... Phosphorylation of CDK4 by Src family kinases with RIPA buffer and the precipitated proteins were resolved by SDS ⁄ PAGE and western blotting Immunodepletion Samples of HeLa whole cell and nuclear ... between fractions 40 and 47, at the start of the phosphate gradient (Fig 2C) Fractions 38–49 and a sample of the input were precipitated using deoxycholate and trichloroacetic acid, and the proteins...

Ngày tải lên: 18/02/2014, 18:20

... formation of ROS, and the phosphorylation of IKK-b and IjB-a at 2, 10 and 30 of stimulation, respectively (Table and Fig 2E,F) An inhibitor for NADPH oxidase attenuates the movement of p47phox ... wavelength of 450 nm Cytokine levels were quantified by comparison with standards The sensitivity of IL-1b and IL-6 was < 0.1 and < 0.7 pgÆmL)1, respectively Data are expressed as the mean ± SEM of triplicate ... NF-jB expression (Fig 2C, lanes and 4) and its DNA-binding activity (Fig 2D, lanes and 5) Phosphorylation of IKK-b and IjB-a was slightly reduced at 2.5 lm of apocynin and eradicated at 12.5 lm (Fig...

Ngày tải lên: 19/02/2014, 00:20

... Ku80, Ku70, histone H1 and 28 k proteins, regardless of the type of damage (Fig 3A, lanes 7–9) In the aggregation of linear and nicked DNA, and high mobility group box (HMGB)1 and HMGB2 proteins ... (lanes and 8) ssDNA induced a noticeable accumulation of 95 k and nucleolinD proteins in addition to DNA-PKcs, Ku80 and Ku70 (lane 9) On the other hand, a relaxed form of closed circular doublestrand ... nucleosomal units of chromatin in equilibrium of ionic conditions [40,41] Probably, the extent of the aggregation would be regulated by DNA damage-responsive chromatin factors, and one of the candidates...

Ngày tải lên: 19/02/2014, 06:20

... NaCl/Pi and washed Fig Biphasic ERK-PP time profile induced by EGF or PMA alone and synergistic ERK phosphorylation induced by EGF and PMA together Cells were serum-starved for three days and subsequently ... combinatory effect of negative feedback and ultrasensitivity [22] Both of these features have been demonstrated experimentally Negative feedback is constituted by the phosphorylation of Sos by ERK, which ... target genes expressed [16], and also affects the type of cellular response that is evoked [17,18] Activation of ERK is required for proliferation of fibroblasts [19] and constitutive ERK activation...

Ngày tải lên: 19/02/2014, 16:20