1 oxidation by fad
Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"
... done by one-way ANOVA followed by post hoc analysis using Bonferroni’s test Values are mean ± S.D for six animals in each group; a p < 0.0 01, a1 p
Ngày tải lên: 26/10/2012, 09:07
Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc
... 249, 11 3 911 55 10 Madison, J.T & Thompson, G.H (19 76) Threonine synthase from higher plants: stimulation by S-adenosylmethionine and inhibition by cysteine Biochem Biophys Res Commun 71, 6846 91 11 ... RI AdoMet Cys Pi [CGS] [TS] JPhser 20 lM 15 lM 10 mM 0.7 lM lM lMặs )1 )1. 55 0 .18 0.06 0.89 )0.7 0. 81 Jcystathionine RJThr I 0.25 )0.03 )0.007 )0 .1 0 .11 1. 03 ể FEBS 2003 An Arabidopsis phosphohomoserine ... control in the plant [12 ]; (d) TS activity is stimulated by AdoMet [10 ,13 15] and inhibited by AMP in vitro [16 ,17 ]; (e) Pi inhibits the activity of both CGS and TS [12 ,17 ]; (f) the enzymatic...
Ngày tải lên: 20/02/2014, 02:21
Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... ATP 0 .1 0 .1 1 0 .1 0 .1 1 kcat,1b (s )1) Ka (mM) kcat,2c (s )1) 2.43 0.08 0.078e 1. 20 0.03 1. 62 0.03 0.076f 1. 054 0.007 0.028 0.005 0 .19 5 0.007 0.0027 0.0004 0 .13 0f 0.22 0. 01 6.4 0 .1 a Assays ... provided by Pierce Chemical Company and with bovine serum albumin as a standard The enzyme concentration was calculated using an Mr of 60 000 per subunit [4] 31 30 60 70 80 90 10 0 11 0 12 0 13 0 14 0 15 0 ... 296, 17 918 7 18 Levitzki, A., Stallcup, W.B & Koshland, D.E Jr (19 71) Half-ofthe-sites reactivity and the conformational states of cytidine triphosphate synthetase Biochemistry 10 , 33 713 378 19 Robertson,...
Ngày tải lên: 21/02/2014, 15:20
Báo cáo khoa học: Inactivating pentapeptide insertions in the fission yeast replication factor C subunit Rfc2 cluster near the ATP-binding site and arginine finger motif docx
... 48–49 49–50 49–57 50– 51 57 64–65 67–68 82–83 94–95 99 10 0 12 4 12 5 12 4 12 5 14 6 14 7 14 9 15 7 15 8 17 0 17 1 17 1 17 2 17 4 17 5 18 3 18 4 19 5 19 6 242–243 2 51 252 254–255 258–259 305 3 31 332 Pro-ArgGlyThrProPro-Arg ... Inserted sequence Identical isolates K10 K12 K17 K32 K33 K33DK27 K33DK22 K27 K22 K23 K23DK14 K3 K14 K18 K19 K9 K 11 K20 F46 F49 F45 K13 F37 F39 K5 F42 K26 K1 F 41 K35 K15 F44 F47 K8 I I I I I I I I I ... changes Low-level expression High-level expression S1 S2 S3 S4 S5 S6 S7 I I I I I I I R95A R101A R165A R95A-R101A R95A-R165A R101A-R165A R95A-R101A-R165A + + + + + + + + + + + + + + presence of thiamine...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: Two overlapping antiparallel genes encoding the iron regulator DmdR1 and the Adm proteins control sidephore and antibiotic biosynthesis in Streptomyces coelicolor A3(2) pdf
... gene cloned into pHZ13 51 dmdR1 gene cloned into pHZ13 51 dmdR1 gene cloned into pRAKn F) recA1 endA2 gyrA96 thi -1 hsdR17 (rk)mk+) sup44relA1k) (u80dlacZDM15) D(lacZYA-argF) U169 dam, dcm, hsdS, ... dmdR1 and adjacent regions in a pHZ13 51 replicon [12 ], and with the new constructs pRAdmdR1 and pHZdmdR1 pRAdmdR1 and pHZdmdR1 were constructed by cloning a 2.2 kb BclI fragment of pSKdmdR1 into ... plasmids STD10 Cosmid containing the dmdR1 gene Redenbach et al (19 96) [42] Stratagene Kieser et al (2000) [38] pBluescript SK pHZ13 51 pRA pRAKn pSKdmdR1 pSKMdmdR1 pSKMadm pHZBH9 pHZdmdR1 pRAdmdR1 E...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx
... regulated by the nitrogen source present in the medium NR activity is suppressed by ammonium and induced when ammonium is replaced by nitrate [12 ,13 ] In the green algae Chlamydomonas reinhardtii [14 ,15 ], ... of pUC18 and sequenced, resulting in pUC18 ⁄ fcp1.9kb (covering 278 bp of fcp coding sequence preceded by 5¢-UTR) and pUC18 ⁄ fcp1.46kb (covering 357 bp of the fcp coding sequence followed by 3¢-UTR) ... 5¢-UTR starting 12 bp upstream of the start ATG, was then cloned into the Eco105I–XbaI sites of pBluescript ⁄ fcp1.9kb, generating pBluescript ⁄ fcp1.6kb, which covers bp )12 to )16 13 upstream of...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III ppt
... 3FEC 1. 0000 10 0 C2 12 2.7, 10 4 .1, 77.6; 10 8.2 1. 0000 1. 2759 1. 0000 12 2.5, 10 4 .1, 77.6; 10 8.0 12 3.0, 10 3.7, 77.6; 10 8.3 12 2.8, 10 4.3, 78.0; 10 7.7 30.0 1. 29 (1. 34 1. 29) 227958 (18 560) 20.0 1. 37 (1. 42 1. 37) ... 25.0 1. 49 (1. 53 1. 49) 14 5908 (9720) 223352 (14 444) 18 1584 (12 632) 12 7549 (9 319 ) 14 2925 (9562) 2292 (15 1) 3806 (246) 13 13 (93) 2983 (18 8) 0 .13 1 (0.220) 0 .14 7 (0. 219 ) 6784 0 .13 3 (0.236) 0 .15 4 (0.256) ... 1. 83 0. 014 0 .12 5 0.020 1. 84 0. 013 0 .13 3 0. 019 1. 86 0. 012 0 .13 2 0.020 1. 88 0. 013 0 .14 0 90.8 8.5 0.5 0.2 13 3 13 5, 327 90.3 9.0 0.5 0.2 13 3 13 5, 327 90.2 9 .1 0.5 0.2 13 3, 327, 5 31 533 90 .1 9.2 0.35...
Ngày tải lên: 23/03/2014, 05:22
Báo cáo khoa học: Perturbation of folding and reassociation of lactate dehydrogenase by proline and trimethylamine oxide potx
... (polar): W,Y 65.668 17 6.5 51 192.795 234.482 2 91. 975 5.249 26.2 31 340.564 704.390 480.940 87.306 14 60.52 79.957 311 .659 Total area represented by sidechains Area represented by hydrophobic sidechains ... Biotechnol 16 , 460–468 Welch, W.J & Brown, C.R (19 96) Influence of molecular and chemical chaperones on protein folding Cell Stress Chaperones 1, 10 9 11 5 10 Tatzelt, J., Prusiner, S.B & Welch, W.J (19 96) ... J Biol Chem 2 41, 24 31 2445 31 Yancey, P.H., Clark, M.E., Hand, S.C., Bowlus, R.D & Somero, G.N (19 82) Living with water stress: evolution of osmolyte systems Science 217 , 12 14 12 22 32 Kelly,...
Ngày tải lên: 23/03/2014, 15:21
Báo cáo khoa học: The N-acetylglutamate synthase/N-acetylglutamate kinase metabolon of Saccharomyces cerevisiae allows co-ordinated feedback regulation of the first two steps in arginine biosynthesis potx
... Strain 14 S31b 14 S31b 14 S31b 14 S31b a (pYB2 (pYB2 (pYB2 (pYB2 + + + + pYX223) pYB3) pHP 21) pHP22) CD, catalytic domain; b ASDb NAGS activity (nmolÆmin )1 mg )1 protein) – + + – – + – + 14 206 10 16 ... harvested by centrifugation, washed and resuspended in minimal medium to a concentration of 10 10 cellsÆmL )1 Drops of 10 lL of 10 -fold serial dilutions (from 10 10 cellsÆmL )1 to 10 5 cellsÆmL )1) were ... the control of a GAL promoter The BY4 /HP73 and HP72/ BY5 primer pairs were used to construct pHP17, BY4 / HP79, and HP80 /BY5 for pHP 21 and BY4 /HP 81 and HP82 /BY5 for pHP22 Plasmids of the pYK series...
Ngày tải lên: 23/03/2014, 21:20
Báo cáo y học: "Chlamydia trachomatis Infection of Human Trophoblast Alters Estrogen and Progesterone Biosynthesis: an insight into role of infection in pregnancy sequelae." doc
... Reprod Fertil Dev 19 95;7: 11 11- 111 6 27 Gagioti S., Colepicolo P., and Bevilacqua E Reactive oxygen species and the phagocytosis process of hemochorial trophoblast Ciencia e Cultura 19 96;48: 37-42 ... Sci 2003 ;10 0: 67 71- 6776 2 31 31 Kam, E.P.Y., Gardner L., Loke Y.W., and King A The role of trophoblast in the physiological change in decidual spiral arteries Hum Reprod 19 99 ;14 : 213 1- 213 8 32 Lunghi ... study JAMA 19 90;263: 319 1- 319 2 13 Beatty W.L., Morrison R.P., and Byrne G.L Persistent chlamydiae: from cell culture to a paradigm for chlamydial pathogenesis Microbiol Rev 19 94;58: 686-699 14 Macauley...
Ngày tải lên: 08/08/2014, 16:23
báo cáo khoa học: " Berry skin development in Norton grape: Distinct patterns of transcriptional regulation and flavonoid biosynthesis" docx
... (FLS) 1. 14 .11 .23 Flavonoid 1. 14 .11 .23 Flavonol synthase (FLS) Flavonol synthase (FLS) 1. 14 .11 .19 Flavonoid 1. 14 .11 .23 Flavonoid 14 VVTU139 81_ at GSVIVT000072470 01 18 10 VVTU2456_s_at VVTU16387_at ... Biology 2 011 , 11 :7 http://www.biomedcentral.com /14 71- 2229 /11 /7 0.045 Page 14 of 23 Catechin Cab Sauv Norton mg/g FW 0.036 0.027 0. 018 0.009 10 0 .12 30 50 70 90 11 0 13 0 70 90 11 0 13 0 70 90 11 0 13 0 Epicatechin ... 2 011 , 11 :7 http://www.biomedcentral.com /14 71- 2229 /11 /7 Page 13 of 23 1. 8 Cab Sauv 2.4 Norton MYBA1 MYBA2 1. 2 1. 8 1. 2 0.6 0.6 0 45 65 85 10 5 12 5 Normalized expression level 0.08 45 65 85 10 5 12 5...
Ngày tải lên: 11/08/2014, 11:21
Báo cáo y học: " Effects of carbohydrate, branched-chain amino acids, and arginine in recovery period on the subsequent performance in wrestlers" potx
... Appl Physiol 2 011 , Doi: 10 .10 07/s004 21- 010 -17 61- 7: 25 Karnincic H, Tocilj Z, Uljevic O, Erceg M: Lactate profile dur ing Greco-Roman wrestling match J Sports Sci Med 2009, 8 :17 -19 26 Huang SY: ... 2000, 72 :10 6 -11 1 Berardi JM, Price TB, Noreen EE, Lemon PW: Postexercise muscle glycogen recover y enhanced with a car bohydr ate-protein supplement Med Sci Sports 24 Exerc 2006, 38 :11 06 -11 13 Williams ... 2000, 11 :11 4 -12 1 40 Betts JA, Williams C, Boobis L, Tsintzas K: Increased car bohydr ate oxidation after ingesting car bohydr ate with added protein Med Sci Sports Exerc 2008, 40:903- 912 41 Iwanaka...
Ngày tải lên: 11/08/2014, 23:21
Manipulation of cyanogenesis and starch biosynthesis in cassava
... 6.3 .1 Assay of enzymes 6.3.2 Activity of major enzymes that control sucrose metabolism 97 97 99 99 99 99 10 0 10 0 10 1 10 1 10 1 10 2 10 3 10 3 10 8 11 7 12 0 13 1 13 3 13 3 13 5 13 5 13 5 13 7 13 7 13 7 13 8 13 8 13 9 ... conclusion and future perspectives 15 0 15 7 15 8 15 8 16 0 16 0 16 1 16 2 16 3 16 4 16 5 16 6 16 6 16 8 16 9 17 0 17 1 17 1 17 6 18 4 19 2 19 5 19 6 19 9 iv List of Tables Table 3 .1 Table 3.2 Leaf position and linamarin ... 58 63 80 88 10 4 10 5 10 7 10 9 11 0 11 5 11 8 11 9 12 1 12 5 12 6 12 7 12 8 14 2 14 3 14 4 14 5 17 2 vi Fig 7.2 Fig 7.3 Fig 7.4 Fig 7.5 Fig 7.6 Fig 7.7 Fig 7.8 Fig 7.9 Fig 7 .10 Fig 7 .11 Photomicrographs of somatic...
Ngày tải lên: 16/09/2015, 17:11
Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc
... 0.7 10 0 12 15 18 21 24 50 12 15 18 21 24 10 0 Fd II 50 12 15 18 21 24 6 12 15 18 21 24 Time of day (h) 50 6 12 15 18 21 24 Time of day (h) 12 15 18 21 24 12 15 18 21 24 50 12 15 18 21 24 10 0 50 ... 50 0 3 50 12 15 18 21 24 10 0 L-FNR Fd VI 12 15 18 21 24 10 0 10 0 50 50 12 15 18 21 24 10 0 10 0 10 0 12 15 18 21 24 50 12 15 18 21 24 10 0 50 10 0 GLU Gln2 50 Gln1-3 10 0 50 0 gdh1 12 15 18 21 24 Fd III ... Fd-GOGAT 1. 5 0.5 12 15 18 21 24 40 àmolãh1ãg1 FW E 12 NADH-GOGAT 6 12 15 18 21 24 Time of day (h) 10 12 15 18 21 24 GDH 25 0.5 0 20 F àmolãh1ãg1 FW àmolãh1ãg1 FW 30 12 15 18 21 24 1. 5 10 GS C...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... (lmolÆmin )1 mg )1) kcat (s )1) kcat ⁄ Km (s )1 M )1) 1. 2 · 10 7 (10 0%) 7.0 · 10 6 (10 0%) 1. 2 · 10 5 · 10 4 2.6 · 10 6 1. 9 · 10 2 (< 1% ) 1. 9 · 10 2 (< 1% ) 3.2 · 10 3 (< 1% ) 2.0 · 10 2 (< 1% ) – – 9.2 · 10 3 dThd 1. 2 ... 6.7 · 10 )3 0 .12 4 ± 0.027 5.5 · 10 )2 ND ND – – 2.8 · 10 4 (< 1% ) 1. 4 · 10 3 (< 1% ) 2.9 · 10 3 (< 1% ) 7.5 · 10 4 (< 1% ) 2.3 · 10 4 (< 1% ) 3.2 · 10 4 (< 1% ) 7.5 · 10 2 (< 1% ) 4.9 · 10 2 (< 1% ) – – [15 ] Y70W ... (< 1% ) 15 .2 ± 0.3 6.8 4.6 ± 0.6 2 .1 2.3 ± 0 .1 1.0 0.82 ± 0.07 0.4 dThd 12 .1 ± 0.5 5.4 dCyd R105H 2 31 ± 24 205 ± 15 14 .7 ± 2.0 6.6 dThd dCyd R105K a [2] b dThd dCyd 8.9 ± 1. 5 11 3 ± 24 ND ND 0. 015 ...
Ngày tải lên: 19/02/2014, 02:20
Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx
... 0.7 1. 4 11 .4 18 .0 24.0 25.0 ± ± ± ± 1. 7 72.6 ± 2.0 2.3* 7.2 ± 0.4** 1. 4 NM 1. 4 NM 0.54 ± 0.5 0.74 ± 0.3 NM NM 0 .1 1.8 3 .1 3.2 10 .15 10 .5 15 6.0 236 ± ± ± ± 2.6 2 .1 5.7 3.9 0.55 0.33 8.6 10 .5 11 .4 ... Sarcoma-bearing mice liver NM EAC 24.8 ± 1. 1 S180 29 .1 ± 0.5 GAMT Arginase II ND 36.7 NM NM 36.7 32.5 34.3 31. 8 ND 49 .1 190.5 18 9.6 NM NM 42 .1 74.5 ± 5.6 ± ± ± ± 0.6 3.5 1. 3 2.5 Estimation of ornithine ... Natl Acad Sci 94, 210 3– 210 5 13 Robertson KD (20 01) DNA methylation, methyltransferases, and cancer Oncogene 20, 313 9– 315 5 14 Van Pilsum JF, Warhol RM, Beckman D & Boline J (19 64) Transamidinase...
Ngày tải lên: 07/03/2014, 04:20
Báo cáo lâm nghiệp: " The role of glutamine synthetase, glutamate synthase and glutamate dehydrogenase in ammonia assimilation by the mycorrhizal fungus Pisolithus tinctorius" ppt
... (GS) activity by the biosynthetic assay, and for NADPH and NADH-dependent GDH activity (Lea, 19 85) After 17 or 18 d growth, the nitrogen-starved were transferred to flasks of fresh 1/ 2MMN medium ... Microbiol 85, Ei9 -17 2 Lea P.J (19 85) In: Techniques in Bioproductivity and Photosynthesis (Coombs J., Hall D.O., Long S.P & Scurlock J.M.O., eds.), Pergamon Press, Oxford, pp 17 3 -18 7 Roon R.R., ... significant role played by GDH References Genetet I., Martin F & Stewart G.S (19 84) Nitrogen assimilation in mycorrhizas Plant PhysioL 76, 395-399 Johnson B & Brown C.M (19 74) Enzymes of ammonia...
Ngày tải lên: 09/08/2014, 04:20
báo cáo khoa học: " Ornithine-δ-aminotransferase is essential for Arginine Catabolism but not for Proline Biosynthesis" ppsx
... Germinating Pea Seeds Plant Physiol 19 83, 73(3):525-528 Page 12 of 14 (page number not for citation purposes) BMC Plant Biology 2008, 8:40 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Slocum RD: Genes, ... water stress Plant Cell Physiol 19 97, 38 (10 ) :10 95 -11 02 Adams E, Frank L: Metabolism of proline and the hydroxyprolines Annu Rev Biochem 19 80, 49 :10 05 -10 61 Delauney AJ, Hu CA, Kishor PB, Verma DP: ... membrane have been identified by complementation of a yeast Arg 11 mutant [11 ,12 ] Pro is mainly synthesised in the cytosol from glutamate (Glu) via pyrroline-5-carboxylate (P5C) by the sequential action...
Ngày tải lên: 12/08/2014, 05:20
Molecular biology of glutamate dehydrogenase and glutamine synthetase in two air breathing teleosts
... Appendix…………………………………………………………………… 10 2 10 2 11 1 11 1 11 1 11 3 11 5 11 6 11 7 11 7 11 7 11 7 12 5 12 6 13 3 13 3 13 7 15 1 15 1 15 3 15 3 15 4 15 5 15 7 15 8 15 9 16 1 16 1 16 2 16 3 16 4 16 7 2 01 iv SUMMARY Air-breathing fishes ... v viii x xviii 1 10 11 11 12 12 13 14 14 15 23 31 31 31 32 33 34 37 37 37 42 42 43 44 47 ii Tissue expression study of gs1 in M albus…………………………… Relative quantification of gs1 by semi-quantitative ... (NP_0 011 34684 .1) , Heterodontus francisci Gs (AAD347 21. 1), Squalus acanthias Gs (AAA 618 71. 1), Paracentrotus lividus Gs (AAC 415 62 .1) , Xenopus laevis GS (NP_0 010 80899 .1) , Xenopus tropicalis GS (AAH6 419 0 .1) ,...
Ngày tải lên: 13/10/2015, 15:54