1 stem cells for cardiac repair

... Paper (Scientist) for the abstract entitled, “Human Bone Marrow- Derived Cardiomyocyte-Like Cells Improve Cardiac Performance In The Infarcted Myocardium" March 2007 Singapore Cardiac Society 19th ... author for the abstract, entitled, “Large Scale Expansion of Human Cardiomyocyte-Like Cells for Cell Therapy” and of poster finalists in the Basic Science category of “Stem/ Progenitor Cells in Cardiac ... Cardiomyocyte-like Cells for Cell Therapy Presenting author at the American Heart Association Scientific Sessions 2005 Poster finalist in the Basic Science category, "Stem/Progenitor Cells in Cardiac Repair" ...

Ngày tải lên: 12/09/2015, 09:42

... 94oC for 0.5 minute The annealing temperature was 64oC (for hVEGF165), 60oC (for Ang-1) and 65oC (for human GAPDH) for 0.5 minute The extension was 72oC for minute In total 30 cycles were performed ... or 71 TRITC positive cells were calculated by counting microscopic fields for stained and unstained cells on slides Dual immunostaining for hVEGF165 and Ang-1 expression For co-expression of ... room temperature for overnight After thorough washing, cells were incubated with 1: 400 goat anti-mouse IgG-FITC (for hVEGF165) and 1: 200 goat anti-rabbit IgG-TRITC (For Ang1) for hour After thorough...

Ngày tải lên: 16/09/2015, 17:14

... replacement as a possible strategy for the CNS is worth considering because stem cells could differentiate into neural cells Neural stem cells would be good candidate for cell replacement in neurodegenerative ... addition, these cells have been referred to as mesenchymal stem cells (MSCs) or mesenchymal progenitor cells for possessing the capability to differentiate into multiple nonhematopoietic cells (Caplan, ... been shown to suppress the function of a broad range of immune cells, including T cells, B cells, NK cells and antigen-presenting cells (Fig 4) and thus may have therapeutic effect in autoimmune...

Ngày tải lên: 11/09/2015, 09:05

... functional cell source for cartilage repair Chondrocytes, fibroblasts, stem cells, and genetically modified cells have all been explored for their potential as viable cell sources for cartilage tissue ... chondrogenic cells Fig 27 Comparison of hESC-derived chondrogenic cells and HCCEC in cartilage repair Fig 28 In vivo cartilage repair at weeks post-implantation Fig 29 In vivo cartilage repair at ... defined numbers, 3000 cells, by centrifugation to form EBs In doing so, a reproducible and robust platform based on hESC differentiation through EB formation was set up for analysis of growth...

Ngày tải lên: 11/09/2015, 09:57

... suggests the biosafety of this combo strategy for cardiac repair and guarantees future study using angiogenic modified skeletal myoblasts for cardiac repair 4.10 Future direction Considering that ... valuable information regarding the effectiveness of combined therapy involving skeletal myoblast transplantation and angiogenic gene therapy for cardiac repair The LCx branch was chosen for ligation ... muscle-derived cells trans-differentiated into cardiomyocytes upon co-culture with contracting cardiomyocytes Skeletal musclederived cells expressed cardiac troponin T and atrial natriuretic peptide, cardiac...

Ngày tải lên: 16/09/2015, 17:14

... ~1x1010 PFU/ ml for Ad-null, ~8x109 PFU/ ml for Ad-VEGF165, ~7x109 PFU/ ml for Ad-Ang-1 and ~5x108 PFU/ ml for Ad-Bic All of the viral vectors were found replication deficient when tested for replication ... 39b & c) isoforms using DMEM injected pig heart tissue as a negative control (Figure 39d & e) and human skeletal muscle as positive controls for slow isoform (Figure 39f) and fast isoform (Figure ... (a) slow isoform and (b) fast isoform (c) A portion of (b) was magnified 800x DMEM injected pig heart tissue was used as a negative control for skeletal myosin heavy chain (d) slow isoform and (e)...

Ngày tải lên: 16/09/2015, 17:14

... maintained at oC for 24h by PNA FISH hESCs maintained at 25 oC for 24h by PNA FISH 33 34 Figure hESCs maintained at oC for 48h by PNA FISH 34 Figure hESCs maintained at 25 oC for 48h by PNA FISH ... of hESCs maintained at oC for 24h by mFISH 37 Figure 11 Karyotype of hESCs maintained at oC for 24h by mFISH 37 Figure 12 Metaphase spread of hESCs maintained at 25 oC for 24h by mFISH 38 Figure ... of hESCs maintained at oC for 48h by mFISH 38 Figure 14 Metaphase spread of hESCs maintained at oC for 48h by mFISH 39 Figure 15 Karyotype of hESCs maintained at oC for 48h by mFISH 39 Figure...

Ngày tải lên: 22/10/2015, 21:13

... 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells share ... culture on 16 transformed cells and in vivo with somatic cells, respectively However, until now this system has not been used on human somatic cells in a cell culture system For neurons and glia, ... easy to engineer so that it can be used for different approaches, and applicable to human stem cells For the application of patient-specific cells, as well as for use with different cell lines, a...

Ngày tải lên: 26/11/2015, 09:54

... the cells were prepared for FACS sorting 96 well plates (Sorenson- 28440) with 10 uL of master mix per well were prepared for single cell sorting 23 For sample/ cell For 100 samples/ cells CellsDirect ... stem cells are hematopoietic stem cells which are formed in the bone marrow These cells play a role by differentiating and replacing the blood cells in the body Other types of adult stem cells ... of some cells which may be critical for the experiment But the methods also require fixing of the cells with 4% Para formaldehyde (PFA) which eventually kills the cells As such, the cells can...

Ngày tải lên: 02/10/2015, 17:14

... to hypoxia ⁄ SD for h The cells were then fixed in 2% paraformaldehyde in NaCl ⁄ Pi for 30 min, washed twice with NaCl ⁄ Pi and permeabilized with 0.3% Triton X-100 in NaCl ⁄ Pi for 10 Next, the ... DMEM for 24 h The cells were then treated with control medium or MSCs-CM [3H]-Thymidine and [3H]-proline uptake assays Cardiac fibroblasts were transferred to 24-well plates, starved of serum for ... 4¢,6-diamidino-2-phenylindone for 20 min, the MSCs were washed in NaCl ⁄ Pi for 10 and mounted in gelvatol for microscopic imaging Protein extraction and western blotting analysis Lysates of stimulated cells were...

Ngày tải lên: 18/02/2014, 04:20

... necessary for LPA-induced Rac activation in B103 neuroblastoma cells [34] Therefore, the possible involvement of Fig LPA stimulates PAK1 in A2058 cells The cells were serumstarved for 12 h and ... stably transfected into A2058 cells by selecting the cells with G418 Cells were washed with phosphate-buffered saline (NaCl/Pi) and grown in complete medium for 36 h before analyses of cell motility, ... activation depends on Cdc42 and Rac1 (A) A2058 cells were serum-deprived and treated with or without 10 lM LY294002 (LY) for h before incubating with LPA (2 lM) for Cellular extracts were incubated with...

Ngày tải lên: 30/03/2014, 13:20

... typing information to narrow down the set of potential donors for a given patient, so these http://www.jiasociety.org/content/12/1/10 individuals could be tested for CCR5-delta32 before costly ... resistance transgenes in stem cells Another approach to the provision of HIV-resistant blood cells is the introduction of resistance-conferring genes into stem cells before transplantation This is ... C46-transfected autologous stem cells in up to 10 patients with an independent indication for autoHSCT, i.e., high-risk AIDS-related lymphoma Use of autologous stem cells for gene transfer currently...

Ngày tải lên: 20/06/2014, 08:20

... receptor) In the blood, mature cells have variable average life spans, ranging from h for mature neutrophils to a few months for red blood cells to many years for memory lymphocytes However, ... accomplish one of three division outcomes: two stem cells, two cells destined for differentiation, or one stem cell and one differentiating cell The former two outcomes are the result of symmetric ... differentiation (Fig 68-1) Stem cells exist to generate, maintain, and repair tissues They function successfully if they can replace a wide variety of shorterlived mature cells over prolonged periods...

Ngày tải lên: 07/07/2014, 01:20

... remove nonadherent cells, and the adherent cells were cultured for days as passage without refeeding The cells were then trypsinized, harvested and resuspended to be used for transplantation ... Eugene, OR, USA) For labeling, the cells were resuspended at × 106 cells/ ml in αMEM, and DiI was added at μl/ml in αMEM After incubation for 20 minutes at 37°C with 5% humidified CO2, the cells were ... stationary for 10 minutes for the cells to adhere (b) Macroscopic observation of cartilage defects after cell transplantation For the control group, the cartilage defect was left empty For the intra-articular...

Ngày tải lên: 09/08/2014, 10:23

... nonadherent cells were removed by a medium change at 72 h and every four days thereafter After two passages, homogeneous MSCs that devoid of hematopoietic cells were used A total of × 106 cells/ ml ... medium at a final concentration of 50 μg/ml for 30 Page of After labeling, cells were washed six times in D-Hanks solution to remove unbound DAPI and then the cells were observed using fluorescent ... the cardiac sample extracted from cardiac tissue of HO-1-MSCs group rather than in the NullMSCs and PBS group Effects of HO-1-MSCs transplantation on angiogenesis Immunofluorescent staining for...

Ngày tải lên: 10/08/2014, 05:21

... epithelial repair by modulating epithelial cell migration and proliferation, but also to the differentiation of repairing cells, leading to the complete restoration of the wounded epithelium [4] Therefore, ... surface, are responsible for lung regeneration and repair [6] Endogenous progenitor cells are also involved in lung regeneration, contributing particularly to the rapid repair of the denuded alveolar ... However, the repair capacity of lungs declines with age, which is primarily due to the endogenous SC failure Therefore, the exogenous stem/progenitor cells, such as embryonic stem cells (ESCs),...

Ngày tải lên: 10/08/2014, 05:21

... with cardiac myocyte specific markers Immunocytochemistry The medium was first removed and the cells were washed twice with PBS, fixed for 30 with 4% paraformaldehyde Cells were permeabilized for ... Regard to the Source of Fetus-Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem cells from whole human umbilical cord ... conditional mediums including cardiac myocytes condition medium (CMCM) plus μM S1P or cardiac myocytes condition medium or DMEM +10% FBS plus μM S1P The medium was changed every days Cardiac differentiation...

Ngày tải lên: 10/08/2014, 05:21

... between the ages for each group (p = 0.076 by ANOVA, mean age + SD: 68.4 + 6.3 for LCNEC, 69.8 + 6.8 for SCLC, and 62.8 + 7.7 for LCC) and the number of male accounts for over 80% for all groups ... obtained with the G-test; the exact p-values for LCNEC vs SCLC were 3.40 × 10-4 for AL1A1, 5.53 × 10-10 for AK1C1, 2.27 × 10-10 for AK1C3, and 0.012 for CD44 The G-test analyses of three cancer ... 0.248 by ANOVA; mean counts + SD: 1916 + 571 for LCNEC, 1879 + 457 for SCLC, 2491 + 645 for LCC) Next, the values of Rsc that is a measure of fold changes for protein expression levels were calculated...

Ngày tải lên: 10/08/2014, 09:22

... PROGENITOR CELLS? TRANSDIFFERENTIATION OF ADULT STEM CELLS PANCREAS-DERIVED STEM CELLS POTENTIAL PITFALLS OF STEM CELL-BASED THERAPIES FOR DIABETES IN HUMANS SUMMARY REFERENCES INTRODUCTION Stem cells, ... transition from cells that stain for both insulin and glucagon in early stage cultures to single hormone-positive cells found in later cultures Ultimately, cells that are reminiscent of true β cells emerge ... conditions used for mouse ES cells, we have obtained similar results with human ES cells Given the longer doubling time of human ES cells, we empirically increased the lengths of both the EB formation...

Ngày tải lên: 10/08/2014, 18:21

... differentiation protocols These advancements will in turn allow for generation of replacement cells for cellular transplantation approaches and for development of the appropriate ‘disease in a dish’ models ... and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305, USA 2Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA Page ... Aldenhoff JB, Laurent LC, Loring JF: A bioinformatic assay for pluripotency in human cells Nat Methods 2011, 8:315-317 96 Birney E, Lieb JD, Furey TS, Crawford GE, Iyer VR: Allele-specific and heritable...

Ngày tải lên: 11/08/2014, 12:21