1 intratubular germ cell neoplasia unclassified

... Features Assay type Cell types Cells enter or cross cellulose membrane Cells move toward and enter capillary filled with attractant Cells avoid capillary filled with control buffer Cells migrate in ... and direction changes of individual cells (together referred to as “tracking” assays) as well as determinations of cell orientation, cell cell interactions, cell morphology, flagellar wave patterns, ... reservoirs; cells adhere to cover glass during migration Cells migrate between cover glass and slide; cells and chemoattractant released from wells Cells migrate across a circular bridge separating cell...

Ngày tải lên: 11/04/2014, 09:43

... Human cell lines used were HeLa cervical carcinoma cells, HepG2 hepatoma cells, K562 and YN-1 erythroleukemia cells, Jurkat T-lymphocyte cells, KG1 myeloid cells, H146 small cell lung cancer cells, ... and HMV-II melanoma cells H146 small cell lung cancer cells were obtained from ATCC (HTB-173) and cultured in RPMI-1640 medium HMV-II melanoma cells were obtained from Riken Cell Bank and cultured ... hemoglobin production in YN-1 and K562 cells [33,34] In fact, the population of hemoglobin-positive cells was about 4.4% in YN-1 cells and 4.9% in K562 cells under basal conditions [19] Otherwise,...

Ngày tải lên: 19/02/2014, 05:20

... previously described [21] However for easier handling of the cells, treatment was performed on HeLa cells instead of CHO K1 cells Cells were then incubated with of lgámL)1 Tat±GFP fusion protein ... same cell lines, namely wild-type (wt) CHO-K1 cells and A-745 mutant cells which are completely defective in HS sulfate expression [21] As a positive control, uptake of the Tat peptide in HeLa cells ... same extent in HeLa cells, in wt-CHO cells and in the A-745 (defective in HS proteoglycan) mutant cell line Moreover internalization appeared to be homogeneous in the whole cell population as...

Ngày tải lên: 21/02/2014, 03:20

... specifically expressed in germ cells of both male and female oysters, and we hypothesized that Oyvlg had a role in germ cell formation [17] However, the function of Oyvlg in germline development had ... reproductive deficiency The premeiotic male germ cells ceased their differentiation before the pachytene In vivo injection of oyvl-dsRNA provokes abnormal germ cell development One month postinjection, ... defects in germ cell development affecting all of the gonadic area, in both females and males Upon histological examination of gonads injected with 20 lg of dsRNA, there were fewer germ cells, and...

Ngày tải lên: 07/03/2014, 00:20

... vitro cell- free methods for protein synthesis with extracts from prokaryotic [1] or eukaryotic [2] cells Wheat germ cell- free eukaryotic protein production offer an alternative to the E coli cell- based ... wheat germ cell- free method comparable to that of the E coli cell- based approach for NMR structure determinations of eukaryotic proteins One of the main advantages of the automated wheat germ cell- free ... normal multinuclear NMR spectra Cell- free protein production protocols are streamlined compared to cell- based protocols, in that they not require cell harvesting or cell lysis Protein purification...

Ngày tải lên: 07/03/2014, 12:20

... testicular Leydig cells in vitro [11] 874 Germ cells constitute the major inner seminiferous tubular cell mass and the role of H2O2 as a signalling molecule in apoptotic induction among germ cells is ... penicillin The cell viability of the final crude population that contained mostly maturing germ cells was in excess of 95% H2O2 treatment The isolated testicular germ cells (5 · 106 cellsÆmL)1 per ... Involvement of Bcl-2 family proteins in germ cell apoptosis during testicular development in the rat and pro-survival effect of stem cell factor on germ cells in vitro Mol Cell Endocrinol 165, 115–129 25...

Ngày tải lên: 16/03/2014, 04:20

... Nonseminomatous Germ Cell Tumor of the Testis 23 Paul H Johnston and Stephen D.W Beck Chapter Diagnostic Imaging of Intracranial Germ Cell Tumors: A Review 47 Takamitsu Fujimaki Chapter Testicular Germ Cell ... Testicular Germ Cell Tumors 75 Delphine Carouge and Joseph H Nadeau Chapter Epigenetic Modifications in Testicular Germ Cell Tumors 107 Christopher J Payne Chapter Claudins and Germ Cell Tumors ... options for the most common type of germ cell tumors of the testes, non-seminomatous germ cell tumors In Chapter 3, Takamitsu Fujimaki reviews intracranial germ cell tumors, which affect mostly...

Ngày tải lên: 17/03/2014, 00:20

... protein N-myristoylation in plant cells in more detail, we established a cell- free protein N-myristoylation system as an application of an advanced wheat germ cell- free translation system that ... Myr–GFP synthesized in the wheat germ cell- free translation system Effect of the combination of amino acids at positions and on protein N-myristoylation in the wheat germ cell- free translation system ... and of the consensus motif in plant target proteins with the use of a Cell- free N-myristoylation of plant proteins wheat germ cell- free translation system We identified several new N-myristoylated...

Ngày tải lên: 29/03/2014, 21:20

... midgestation of putative germ cell- derived clones, we hypothesize that either gonadal cells other than germ cells may have been picked instead of true germ cells or that a subset of germ cells may not have ... developmental potential of germ cell nuclei after transfer into oocytes remains controversial To date, gonadal cells bearing morphological resemblance to germ cells have been used or germ cells have been ... donor cells, either ES, EC, or germ cells could be used In this study, germ cells are used as they match cumulus cells for direct recovery from the body and absence of in vitro culture Gonadal cells,...

Ngày tải lên: 11/04/2014, 09:43

... of renal tubules representing the origin of clear cell renal cell carcinoma (RCC) and in the regulation of the cell cycle of parietal epithelial cells of the Bowman's capsule [10,11] Since UCHL1 ... degradation pathway plays an important role in distinct cellular processes such as cell proliferation, cell cycle, apoptosis and intracellular signalling [8], which are often disturbed in cancers ... the expression of UCHL1, RCC cell lines were treated for days with 1, and 10 μM DAC (Sigma-Aldrich GmbH, Taufkirchen, Germany) Subsequently untreated and DAC treated cells were harvested, lysed...

Ngày tải lên: 18/06/2014, 15:20

... on fusion of TF228.1.16 cells with SupT1 cells Effect of3 Effect of IM28 on fusion of TF228.1.16 cells with SupT1 cells TF228.1.16 cells were mixed with SupT11 cells (1:1 cell ratio) and transferred ... TF228.1.16 cells were mixed with 293/CD4+ cells (1:1 cell ratio) and transferred to a 24-well plate (105 cells per well in 200 µl of culture medium) TF228.1.16 cells and 293/CD4+ cells were incubated ... HIV-1-infected cells to naive cells We found that IM28 was able to inhibit cell- cell fusion in an in vitro system We showed that IM28 significantly blocks HIV-1 glycoprotein-mediated cell- cell fusion...

Ngày tải lên: 18/06/2014, 22:20

... on fusion of TF228.1.16 cells with SupT1 cells Effect of3 Effect of IM28 on fusion of TF228.1.16 cells with SupT1 cells TF228.1.16 cells were mixed with SupT11 cells (1:1 cell ratio) and transferred ... TF228.1.16 cells were mixed with 293/CD4+ cells (1:1 cell ratio) and transferred to a 24-well plate (105 cells per well in 200 µl of culture medium) TF228.1.16 cells and 293/CD4+ cells were incubated ... HIV-1-infected cells to naive cells We found that IM28 was able to inhibit cell- cell fusion in an in vitro system We showed that IM28 significantly blocks HIV-1 glycoprotein-mediated cell- cell fusion...

Ngày tải lên: 20/06/2014, 04:20

... 4.0% for renal cell carcinoma, 0.84% to 3.6% for transitional cell carcinoma, 0.4% for germ cell tumors, and 0.36% to 0.7% for prostate adenocarcinoma[9] Testicular mixed germ cell tumors are ... testicular mixed germ- cell tumors is because germ cells in the testes are totipotent and can undergo either trophoblast or somatic differentiation Among all the subtypes of testicular germ cell tumor, ... Cutan Pathol 2009, 37(4):486-90 16 International Germ Cell Consensus Classification: A prognostic factor based staging system for metastatic germ cell cancers J Clin Oncol 1997, 15:594-603 doi:10.1186/1477-7819-8-21...

Ngày tải lên: 09/08/2014, 03:21

... (Figure 1c) The diagnosis was of an International Germ Cell Cancer Collaborative Group (IGCCCG) poor prognosis metastatic nonseminomatous germ cell tumor Four cycles of bleomycin, etoposide and ... European Consensus Conference on Diagnosis and Treatment of Germ Cell Cancer: A report of the Second Meeting of the European Germ Cell cancer Consensus group (EGCCCG): Part Eur Urol 2008, 53:478-496 ... indicates a poor prognosis within the IGCCCG classification [3] The spread of nonseminomatous germ cell tumors may be local, via the lymphatics or via vascular channels Review of our patient’s...

Ngày tải lên: 11/08/2014, 03:20

... CD4+ T cells We have previously observed an increase in naïve CD4+ and CD8+ T cells, a significant increase in memory/effector CD8+ T cells, as well as recovery of natural killer (NK) cell numbers ... for CD4 T cell help in generating functional CD8 T cell memory Science 2003, 300(5617):337-339 Sun JC, Bevan MJ: Defective CD8 T cell memory following acute infection without CD4 T cell help ... and mean ± sem CD4+ T -cell count was 478.4 ± 55.6 cells/μl of blood and throughout the study viral load remained detectable in only one patient, whilst CD4+ and CD8+ T -cell counts remained unchanged...

Ngày tải lên: 11/08/2014, 10:23

... Matsuda H: Primary germ cell tumors in the mediastinum: a 50-year experience at a single Japanese institution Cancer 2003, 97(2):367-376 Droz JP, Horwich A: Extragonadal germ cell tumors Comprehensive ... patient’s pituitary showing polymorphic cells with granular cytoplasm Discussion This case study is unusual because our patient had both SLE and pituitary germ cell tumor It is also unusual because ... Rheum 2003, 48:2100 doi:10.1186/1752-1947-4-97 Cite this article as: Shahrir et al.: Extragonadal germ cell tumor presenting in a woman with systemic lupus erythematosus: a case report Journal of...

Ngày tải lên: 11/08/2014, 12:20

... cell- to -cell transmission p24 10 10 p / low np / low 10 10 10 E Cell- to -cell p / Wt, low 10 10 Cell - free Cell number np / Wt, high 10 GFP Cell - free 10 10 np / Wt, low 10 0.3% p24 p24 10 Cell- to -cell ... 2C) infected H9 (np) donor cells or cell- to -cell transmission from weakly infected MT-4 (p) cells (Fig 2D) are shown The respective mean percentage levels of cell- tocell transmission of HIV-Env-Tr712 ... a celltype specific defect such that replicative spread occurs in some T -cell lines (here MT-4 cells, termed permissive (p) cells) but fails to occur in the majority of T -cell lines (here H9 cells,...

Ngày tải lên: 12/08/2014, 23:23

... apoptosis, modulate viral transcription, and alter cellular chemotaxis in infected cells [39,40] Downregulation of cell surface CD4 by Nef in primary CD4+ T-cells by unintegrated DNA is well established ... downregulates cell surface CXCR4, CCR5 and CD4 expression on Rev-CEM cells The Rev-CEM cell line was derived by transducing the Rev and Tat dependent GFP vector pNL-RRE(SA) [18] into CEM-SS cells, resulting ... seen with integrated infections of TZM/bl cells [36] Studies with 293-Affinofile cells, a cell line that is quantitatively inducible for both CD4 and CCR5 cell surface expression, revealed that...

Ngày tải lên: 12/08/2014, 23:23

Ngày tải lên: 13/08/2014, 01:20

... increase of cells in G2 phase of cell cycle in p30 Jurkat T-cells of independent experiments was calculated by dividing the number of cells in G2 phase of cell cycle in p30 Jurkat T-cells over ... apoptosis in 293T cells or Jurkat T-cells HTLV-1 p30 does not modulate apoptosis in 293T cells or Jurkat T-cells A) p30-expressing Jurkat T-cells or mock infected Jurkat T-cells were treated ... after release, cells started to enter the G2/M phase of cell cycle in both p30 expressing and mock Jurkat Tcells However, as compared to mock transduced cells, p30 transduced Jurkat T-cells had a...

Ngày tải lên: 13/08/2014, 05:22