— characterization of environmental parameters affecting microbial kinetics in foods

... Monitoring of VOCs in industrial areas during night times and also extending monitoring in the surrounding villages APPCB will ensure the linkage of Continuous Ambient Air Quality Monitoring Stations ... sweeping: The industry has procured a road sweeping machine with a cost of Rs 0.25 Cr in the year 2004 and cleaning the internal roads d Online monitoring: The industry has provided stack monitoring ... thereby reducing the movement of M/s VPT is in the process of number of trucks and dust fly-off mechanization of stocking, loading and unloading of dusty cargo like coal and iron ore to eliminate the...

Ngày tải lên: 06/03/2014, 16:20

... provided in Fig 1B (insert), which shows the characteristic cross peak from the geminal protons of carbons and of glycerol in TG [1] Cross peaks of lipids, including the T signal, were absent in cells ... where the relevant increase of m2 produces the relevant intensity changes of ML TG signals In principle, such an increase can be due to both a decrease in c1 and an increase in p1 after growth ... indicating the different equilibrium of catabolism versus anabolism Table reports on the intensity of changes of the choline-based metabolites for the two cell lines after irradiation resulting...

Ngày tải lên: 18/02/2014, 13:20

... (S)-cheilanthifoline, not (S)-nandinine [7,30,31] If we assume that (S)-nandinine is produced in E californica, there may be no need for a catalyst from (S)-nandinine to (S)-stylopine However, nandinine could ... and the flow rate (0.5 mLÆmin)1) To determine the kinetic parameters of CYP719A2, the amount of nandinine produced was estimated by using a calibration curve of (S)-scoulerine (pmol versus peak area) ... determine the kinetic parameters of CYP719A3, the amount of nandinine produced was estimated by using the calibration curve of (S)-scoulerine (pmol versus peak area) and the absorption ratio of...

Ngày tải lên: 07/03/2014, 10:20

... product of the second round of PCR was cloned and the 1066 bp sequence encoding 125 amino acids of the A-chain, the 19 amino acids linker, and 216 amino acids of the B-chain of the ml gene was obtained ... However, the amino acids forming the catalytically active center of the A-chains of the MLp toxins are fully conserved and retained in corresponding positions, as in the A-chain of ricin [27] and ... contain a 33 amino acid N-terminal leader peptide and a small linker peptide joining the A- and B-chains – these are removed during protein maturation Comparison of amino acid sequences of the...

Ngày tải lên: 07/03/2014, 15:20

... Comparison of the fasciclin I domains in CLAP with selected proteins in the protein database The Conserved Domain Database of NCBI was screened with the protein coding sequence of clone of CLAP ... as adhesin [40] Adhesin is a heterodimer composed of a cathepsin L-like protease and a protein with an adherence domain containing four glycosylation sites Adhesin promotes the binding of E histolytica ... domains or putative GPI unit, if one exists in CLAP as it does in Drosophila fasciclin I and many other fasciclin-containing proteins [36,46] Fas I domains in proteins are almost always found in...

Ngày tải lên: 07/03/2014, 16:20

... approximately 90% of artemin-containing cells endured a 30 heat shock, as opposed to only 17% of those lacking the protein (Fig 4A) In comparison, 55% of cells containing artemin survived incubation in 0.50 ... and in comparison to the protein in cyst extracts, suggesting that it protected transfected cells in a way other than by indiscriminate binding to denaturing proteins In this vein, Artemia p26 inhibits ... Function of an Artemia ferritin homolog A B C Artemin, apoferritin and ferritin inhibit citrate synthase denaturation Artemin, apoferritin and ferritin protected citrate synthase against denaturation...

Ngày tải lên: 16/03/2014, 11:20

... homologs of SpDlp1 We also showed that both components are involved in determination of the isoprenoid chain length of ubiquinone Results Isolation and sequence analysis of genes encoding murine and ... sequences of known long-chain-producing trans-prenyl diphosphate synthases (B) Alignment of the amino acid sequences of a partner protein present in the long-chain trans-prenyl diphosphate synthases of ... component is more important in determining the chain length of ubiquinone by replacing either component with homologs from other species and analyzing the type of ubiquinone produced FEBS Journal...

Ngày tải lên: 16/03/2014, 23:20

... nonreducing end of the oligosaccharide Absence of RCA-I binding to HvALP suggested lack of terminal galactose, confirming that SBA and WFL were binding to a terminal GalNAc residue Terminal GalNAc in ... is part of the C group of binding sites According to Ó FEBS 2004 Heliothis phosphatase and Cry1Ac binding (Eur J Biochem 271) 3133 the current toxin binding model, alteration of C binding sites ... the presence of N-linked oligosaccharides on HvALP Binding to other Cry1Ac binding proteins was also decreased greatly by PNG-F digestion, indicating the importance of N-linked protein glycosylation...

Ngày tải lên: 23/03/2014, 13:20

... assembly in the presence of metals is unlikely to involve the same intermolecular interactions as within fibrils, as removal of the metals causes dissociation into monomers instead of the continued ... Error bars indicate the experimental uncertainty of the measurements observed between the Cu2+-induced and Zn2+-induced aggregates, reflecting the differences in metal-binding properties of Ab [26,30,33–35] ... the inherent light scattering of aggregates (data shown only for 50 lm) All forms of aggregates were examined using AFM (Fig 2) The results were in accordance with previous investigations in which...

Ngày tải lên: 30/03/2014, 01:20

... to fragmented adhesion region in the cell center Vinculin staining on the whole cell surface in most of the cells (green, G); some cells still have vinculin staining similar to starved cells (arrow) ... phosphorylation was inhibited by  70%, returning to the level observed after the addition of fresh medium in the absence of agonist (Fig 6C, inset) Incubation in media containing specific receptor ... Association of increased phosphatidylinositol 3-kinase signaling with increased invasiveness and gelatinase activity in malignant gliomas J Neurosurg 95, 480–488 Vajkoczy P, Goldbrunner R, Farhadi M, Vince...

Ngày tải lên: 30/03/2014, 08:20

... was indeed only transferred to the active site phosphopantetheine thiol of the ACP domain The increase in labeling of the ACP-CON didomain may be a result of improved folding of the ACP domain ... yield of the soluble protein Pantetheinylation of the ACP in ACPCON didomain was verified using MALDI-TOF of a tryptic fragment of the purified protein (data not shown) The LovB CON domain can ... proteins in pure forms N-terminal sequencing of the 45 kDa protein indicated it is indeed the standalone MAT domain The truncation site is at EY448|M449EPEQ (N-terminal sequencing results are indicated...

Ngày tải lên: 30/03/2014, 08:20

... showing that the growth of a thin GaP layer on the top of InGaP, before GaAs is grown, is effective in preventing the formation of the quaternary interlayer because In segregates into the interposed ... HAADF intensities of In0 .15Ga0.85As1-yPy and GaAs Inset is the top left part of the plot according to our results In fact, this mechanism would entail the incorporation of As in the bottom InGaP ... the formation of some InGaAsP alloy inside the nominal InGaP layer, with the consequent broadening of the interface towards both the nominal InGaP and GaAs layers These detailed investigations...

Ngày tải lên: 21/06/2014, 05:20

... selected strains of turkeys, referred to as strains A, B and C Strains A and B are female lines Strain C is a male line, which produces tom turkeys for matings at the final stage of a crossbreeding scheme ... during the rearing period; others were eliminated at the weighing times The causes for removals were diverse and not recorded Incidences of eliminations were 1, 0.3 and 3% for females in strains ... for males in the same strains The higher removal rate in strain C was likely a result of the intense selection carried out, mainly on weight criteria, as is common in heavy turkey strains Unfortunately,...

Ngày tải lên: 09/08/2014, 18:22

... selected strains of turkeys, referred to strains A, B and C Strains A and B are female lines Strain C is a male line, which produces tom turkeys for matings at the final stage of a crossbreeding scheme ... Still, in both lines A and C, whatever the model applied, BW16 was genetically more m correlated with BW12 (0.88 in line A vs 0.82 in line C) than with BW16 (0.83 f f in line A vs 0.78 in line C) In ... for males in the same strains The higher removal rate in strain C was likely a result of the intense selection carried out, mainly on weight criteria, as is common in heavy turkey strains Unfortunately,...

Ngày tải lên: 09/08/2014, 18:22

... al Journal of Clinical Bioinformatics 2011, 1:8 http://www.jclinbioinformatics.com/content/1/1/8 staining compares the relative intensity of staining of one core of a TMA to that of a control ... score of indicates faint staining, indicates medium intensity staining, and indicates dark staining Furthermore, the pathologist is also able to visually assess the localization of predominant ... serving as a proxy for higher protein expression Two measures, the percent and intensity of IHC staining, were used to describe the level of protein expression in a tissue sample Percent staining...

Ngày tải lên: 10/08/2014, 09:22

... are indicated in lower case Overlapping sequences found in deletion sites are highlighted in bold Inserted sequence is highlighted in bold and Italic - 28 - Table Classification of mutations induced ... Local Lesions in Genomes (TILLING) [5-9] Because of its mutation-inducing property, EMS is also very useful for producing leaky alleles in forward genetics By contrast, X-rays and γrays induce DNA ... five of the six breakpoints of rearrangements had deletions, whereas no breakpoint contained a duplication (Figure 3) A previous study revealed that the breakpoints induced by C ions with LET of...

Ngày tải lên: 11/08/2014, 11:21

... quenching (qE) of excess light involves a ΔpH-induced activation of the xanthophyll cycle in which a violaxanthin de-epoxidase converts violaxanthin (V) into antheraxanthin (A) and zeaxanthin (Z) ... resulting in higher expression of the GPXH gene Increased oxidative stress resistance of many of these mutants confirms the importance of the O2-induced genetic response in the defense against ROSinduced ... changes (Figure 5C) A general increase in antioxidant levels including zeaxanthin, antheraxanthin, lutein and a-tocopherol and a significant rise in NPQ was detected in strain 14H8, where no stimulated...

Ngày tải lên: 11/08/2014, 11:21

... genes in early patterning of inflorescence architecture and in maintenance of the inflorescence meristem [32,33] The BEL domain of BLH directly interacts with the MEINOX domain of KNOX, and the interaction ... determine the KNOX-interacting domain of wheat BLH proteins, deletion-mutant constructs of WBLH1 and WBLH2 were produced, and protein-protein interaction of the WBLH1 and WBLH2 deletion proteins ... Summary of the results of the yeast two-hybrid assay Circles and crosses respectively indicate interacting or activating, and noninteracting or nonactivating N.D., not determined Interaction of BD-WBLH1...

Ngày tải lên: 11/08/2014, 11:21

... resolution of the lectin binding results The trachea of the duck and geese showed no a2,6 SA The virus-binding assay showed no binding to the Table Hemaglutinin binding affinity of H3N2 viruses Horse ... another may bind exceedingly well [15] Unfortunately, both will show a positive reaction to the lectin-binding assays Thus, determining the influenza virus-binding profile in tissues of different ... glycan terminating in a2,3 SA while S nigra agglutinin binds to terminal a2,6 SA [12,13] Here we use two methods of lectin staining to describe the distribution of a2,3 SA and a2,6 SA in six poultry...

Ngày tải lên: 11/08/2014, 21:21

... Clin Microbiol 2004, 42:2455-2460 41 Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors 1977 Biotechnology 1992, 24:104-108 42 Ewing B, Green P: Base-calling of ... carried out to investigate the clinical, virological and therapeutical response characteristics of HBV genotypes, using a large number of samples, including patients representing Rondônia State ... manuscript and in its design and coordination LB participated in the PCR amplification and sequencing process DSV participated in the design of the study JRRP participated in the elaboration of the manuscript...

Ngày tải lên: 12/08/2014, 02:20