fatty acid production and stress tolerance

... Parvaiz Ahmad, and Nicole Geissler Prospects of Halophytes in Understanding and Managing Abiotic Stress Tolerance Vinayak H Lokhande and Penna Suprasanna UV-B Radiation, Its Effects and Defense ... 2008) Oxidative Stress and Antioxidative Responses to Environmental Stress 3.1 Production of ROS Environmental stresses are responsible for the production of ROS The production and removal of ... osmoprotectants, and antioxidants Stress- responsive genes and gene products including proteins are expressed and provide tolerance to the plant To understand the physiological, biochemical, and molecular...

Ngày tải lên: 14/03/2014, 10:20

... α-linolenic acid; ARA: Arachidonic acid; CVA: canonical variance analysis; DHA: docosahexaenoic acid; DMOX: 4, 4-dimethyloxaline; EPA: Eicosapentaenoic acid; FA: fatty acid; FAME: fatty acid methyl ... of FAs Out of the 76 fatty acids, 36 substances were identified by their mass spectrum and by retention time according to a standard substance, and the other 40 fatty acids were identified by their ... performed the statistical analysis IF and TF conceived of the study, and participated in its design and coordination and helped to draft the manuscript All authors read and approved the final manuscript...

Ngày tải lên: 11/08/2014, 11:21

... fatty acid; ALA, a-linolenic acid, 18:3n-3;EPA, eicosapentaenoic acid, 20:5n-3; DPA, docosapentenoic acid, 22:5n-3; DHA,docosahexaenoic acid, 22:6n-3; LA, linoleic acid, 18:2n-6; AA, arachidonicacid, ... Hydroxyeicosatetraenoic acid HPETE Hydroperoxyeicosatetraenoic acid PG Prostaglandin TX Thromboxane DPA Docosapentaenoic acid RCT Randomized controlled trial IX Chapter 1: Introduction and literature ... docosahexaenoic acid, DiHDHAdihydroxy-docosahexaenoic acid, DiHp-ETE dihydroperoxy-eicosatetraenoic acid, EPA eicosapentaenoic acid, HETE hydroxy-eicosatetraenoic acid, HpDHAhydroperoxy-docosahexaenoicacid,...

Ngày tải lên: 30/09/2015, 10:11

... fatty acids, and the ratio of each fatty acid RESULTS AND DISCUSSION Characteristics of carbon stable isotope in Z japonica The mean δ13C of Z japonica was -12.4 ± 1.6‰ in the leaves and stems, and ... the roots and rhizomes Meanwhile, Z marina in the leaves and stems has -9.7 ± 1.0‰, and in the roots and rhizomes has -9.6 ± 1.0‰ Fig shows that the leaves and stems as well as the roots and rhizomes ... We found about 30 kinds of fatty acids in the leaves and stems and about 36 kinds of fatty acids in the roots and rhizomes of Z japonica We focused on the fatty acid content of Z japonica , because...

Ngày tải lên: 05/09/2013, 10:17

... hydroperoxides increases the risk of polymerization and acidification and of the appearance of insoluble sediments and gums, which can lead to filter plugging and deposits in the fuel systems Esterified ... of Energy and Environment (IJEE), Volume 1, Issue 3, 2010, pp.411-430 427 properties and fatty acid composition on CO and HC emissions The correlation analysis reveals that the CO and HC emissions ... burning phase and an increase in the production of NOX [15] The advance in injection timming was further investigated and concluded that a carbon –carbon double bond introduces a kink into, and thereby...

Ngày tải lên: 05/09/2013, 15:28

... 38 and 22.1 ( 15.2 g -carotene/g FW in extracted samples of aril and mesocarp, respectively, and 16 and g/g of zeaxanthin and 35 and g/g of -cryptoxanthin in saponified samples of mesocarp and ... relative standard deviation, % vaccenic (in two fruit), R-linolenic, and eicosa-11-eneoic acids were also detected The fatty acid composition of the aril and seed are interesting, and they reflect ... Internal standard (nonadecanoic acid methyl ester) was added, and the extraction took place at 55 °C for 30 with shaking every 10 Extracts were filtered and dried over sodium sulfate, and the solvent...

Ngày tải lên: 09/02/2014, 00:08

... Fernandez, Sergio Alemano, Ana Vigliocco, Andrea Andrade, and Guillermina Abdala 12 An Insight into the Role of Salicylic Acid and Jasmonic Acid in Salt Stress Tolerance ... S, C, and G, respectively, were also involved in salt and freezing stresses These proteins bind to ABRE and couple of other cis-acting elements with differential affinity and improve stress tolerance ... BR-treated plants Oxidative stress- related genes encoding monodehydroascorbate reductase and thioredoxin, the cold and drought stress response genes COR47 and COR78, and the heat stress- related genes...

Ngày tải lên: 14/02/2014, 12:20

... cation ligands are main-chain oxygen atoms, and three are the side-chain oxygen atoms of N296, E342 and S387 The glutamic acid and asparagine residues are conserved among known KAS I and KAS II ... mutants K328H and K328R are more efficient (247% and 227%), the acidic mutant K328E is less efficient (31%), and the bulky mutants K328F and K328I are similar to the wild-type (91% and 117%) Increasing ... two basic mutants, K328H and K328R, and the bulky mutant, K328F, evinced some activity (Fig 7A, lanes 7–12, and Fig 7B, lanes 2–4) By comparison, the acidic mutant K328E and to a lesser extent...

Ngày tải lên: 19/02/2014, 08:20

... represented as fabp6 and Fabp6, respectively, human genes and proteins are given in upper-case letters, e.g FABP6 and FABP6, respectively, and the mouse gene is designated Fabp6 and its protein FABP6 ... chromosome 21 and human chromosome are located on rat chromosome 10 and mouse chromosome 11 Other genes are located on rat chromosomes 2, 17, 18 and 20, and mouse chromosomes 13, 15 and 18, suggesting ... [7], and extensive studies have focused on the tissue distribution and binding activities of FABPs and regulation of FABP genes, including FABP gene knock-out experiments [8], our understanding...

Ngày tải lên: 07/03/2014, 06:20

... and M Riscoe (VAMC, Portland, OR, USA), and assayed as described previously [28] pH prole CFAS activity was determined as described above in the following buffers: 4-morpolinoethanesulfonic acid ... enzyme overproduction J Bacteriol 158, 286295 Wang, A., Grogan, D.W & Cronan, J.E (1992) Cyclopropane fatty acid synthase of Escherichia coli: deduced amino acid sequence, purication, and studies ... MacLean, D.B (1980) The biosynthesis of cyclopropane faty acids I Feeding experiments with oleic acid- 9,10-d2, oleic acid- 8,8,11,11-d4, and 1-methionine-methyl-d3 Can J Chem 59, 828838 18 Buist,...

Ngày tải lên: 07/03/2014, 16:20

... New BioLabs and Gibco BRL Pfu DNA polymerase and alkaline phosphatase were from Promega and Boehringer Mannheim, respectively D-Amino acid oxidase (EC 1.4.3.3) from porcine kidney and horseradish ... The amount of D-amino acids produced was determined by the Chirobiotic T HPLC method and the appropriate D-amino acids were used as the standards RESULTS Identification and phylogenetic analysis ... N-acyl-D-amino acid amidohydrolase (56.7% identity and 63.6% similarity), N-acyl-D-glutamate amidohydrolase (44.8% identity and 51.2% similarity) and N-acyl-D-asparate amidohydrolase (48.5% identity and...

Ngày tải lên: 08/03/2014, 16:20

... physiological roles for FABPs include the uptake and utilization of fatty acids, intracellular targeting of fatty acids to specific organelles and metabolic pathways, and the protection of cellular structures ... highest sequence identity and similarity (48% and 74%, respectively) with shark FABP10, and the next highest sequence identity and similarity with zebrafish Fabp10a (46% and 75%, respectively) (Fig ... in muscle and heart were 6–24 times higher than in brain, eye, gills, testis, skin, kidney and swimbladder, and 320–650 times higher than in liver, intestine and ovary Both RT-PCR and RT-qPCR...

Ngày tải lên: 16/03/2014, 00:20

... palm, olive, corn or fish oil, and the TG (A) and TC (B) accumulated in the cells was measured after 5, 24 and 48 h Data are the mean from three separate experiments, and error bars show the SEM ... at 13 h (P < 0.01) and 24 h (P < 0.01) Corn and fish CRLPs were taken up at very similar rates, and there were also no significant differences in the rates of uptake of palm and olive CRLPs Fig ... are enriched in SFAs, MUFAs, n)6 PUFAs and n)3 PUFAs [23], as well as containing a range of other fatty acids, and these enrichments influence the uptake and metabolism of the particles by the...

Ngày tải lên: 16/03/2014, 12:20

... putative ligands of MvFABPs, their equilibrium dissociation constants and pH dependence, 3D structure, subcellular localization, and the mechanism of fatty acid transfer between FABPs and phospholipid ... fatty acid transfer from adipocyte and heart fatty acid binding proteins by acceptor membrane lipid composition and structure J Biol Chem 269, 10517–10523 47 Hsu KT & Storch J (1996) Fatty acid ... Mvfabpa and Mvfabpb, were identified as being similar to those for fatty acid binding proteins (Fig 1A,B) They share 65% identity at the nucleotide level and 62% identity at the amino acid level...

Ngày tải lên: 23/03/2014, 07:20

... Vertebrate FABPs are involved in cellular fatty acid transport and utilization and compartmentalization of intracellular fatty acid storage, and also in fatty acidinduced regulation of gene expression ... similarity in the ligand-binding pocket to bovine and human CRABP [5] However, recent ligand-binding studies with recombinant msCRABP showed high affinity binding for fatty acids and negligible interaction ... Soderhall et al ¨ ¨ A B Fig Nucleotide sequence and deduced amino -acid sequence of plFABP and pmFABP (A) P leniusculus FABP cDNA nucleotide and putative amino -acid sequence The putative polyadenylation...

Ngày tải lên: 23/03/2014, 10:21

... summarized below, focused on one hand on the biogenesis and stability of the variant K304E MCAD protein, coded from the 985AdefiGua allele, and on the other hand on the activity and chain-length selectivity ... will illuminate considerations and ideas that are applicable to the other fatty acid oxidation deficiencies and many other genetic disorders Genomic structures and disease-associated sequence ... of fatty acid oxidation The draft sequence of the human genome was published in 2001 [7,8] and the assembly of large contigs and the annotation of genes makes it possible to find gene and genome...

Ngày tải lên: 23/03/2014, 12:20

... acid, the fatty acid is oxidized to yield a pentyl radical [27] and 12-oxododecylcis-9-enoic acid [28] via b-scission of hydroperoxide intermediates, leading to production of 1-hexanal [29] and ... saturated fatty acids (SFAs) and unsaturated fatty acids (USFAs) including linoleic acid at an incipient stage of cultivation and consume them with concomitant formation of lipid hydroperoxide and TBARS ... products and authentic 19 aldehydes and seven ketones demonstrated that glyoxal, 1-hexanal and 1-pentanal were formed from linoleic acid by MnP in any of the buffer systems (Fig 1) Syn and q FEBS...

Ngày tải lên: 24/03/2014, 04:21

... Fig Effect of leptin and progesterone on FAAH activity and expression (A) Effect of leptin on the activity of FAAH in human U937 and CHP100 cells and on the protein content and the mRNA of FAAH ... Francke, U (1998) Conserved chromosomal location and genomic structure of human and mouse fatty -acid amide hydrolase genes and evaluation of clasper as a candidate neurological mutation Genomics 54, ... activity and expression was additive, in fact treatment of U937 cells for 24 h with 10 nM leptin and lM progesterone enhanced FAAH activity, protein level and mRNA content up to  500%, 450% and 490%...

Ngày tải lên: 30/03/2014, 15:20

... percentage amino acid identity between zebrafish and human, mouse and pufferfish B-FABP is 83%, 76% and 83%, respectively The percentage amino acid identity between zebrafish and human and mouse is ... amino acid identity and dashes a deletion/insertion The percentage amino acid sequence identity for the peptides encoded by each exon of the B-FABP gene between zebrafish and human, mouse and pufferfish ... testes and between 50 and 160 times higher in brain than in muscle, intestine and heart No product was generated by qRT-PCR from liver, ovary, skin and kidney RNA Both conventional RT-PCR and qRT-PCR...

Ngày tải lên: 31/03/2014, 07:20

... The minor band (Fig 2) was reamplified by PCR and repurified The purified DNA fragments were cloned into the plasmid vector, pGEM-T (Promega, Madison, WI, USA), and three of the major band and one ... AW077983) Both strands of the cDNA clones were sequenced, aligned and the amino acid sequence for the zebrafish fabp3 was deduced The coding nucleotides are shown in uppercase and the 5¢ and 3¢ UTRs ... gel-electrophoresis Three bands of  250 bp, 200 bp and 180 bp in the TAP+ reaction were excised from the gel and purified using the Qiaquick gel extraction kit (Qiagen) The two minor bands ( 250 bp and  180...

Ngày tải lên: 31/03/2014, 07:20