expression of aquaporin genes in am plants under drought stress conditions

... coli: streptomycin (50), ampicillin (100), kanamycin (50), gentamycin (20), erythromycin (50); for T roseopersicina: streptomycin (5), kanamycin (20), gentamycin (5) erythromycin (50) Conjugation ... role of the N-terminal part of the kinase, containing a PAS domain, was established in signal transduction between the RH and the kinase [6,7] Addition of H2 to HupUV before or during the incubation ... (upstream region of the hupU) was inserted into the 5924-bp BamHI vector fragment of pTUV2 (containing the downstream region of the hupV), resulting in pHUVD1 The 4534-bp KpnI–XbaI fragment of 4814 ´...

Ngày tải lên: 16/03/2014, 23:20

... the aim of increasing productivity One such mechanism relies on the inhibition of dominant amplification m a r k e r s such as glutamine synthetase or adenosine deaminase by the addition of specific ... as neomycin or hygromycin, positive transfectants can be selected in media containing the corresponding antibiotic Induction of gene amplification using glutamine synthetase22 or dihydrofolate ... points following infection with varying MOIs Analysis of recombinant protein production in these samples will indicate how much virus is needed to obtain maximal yield after a given time of infection...

Ngày tải lên: 11/04/2014, 09:41

... The use of fenofibrate was related to significant decreases in the expression in circulating leukocytes of CX3CL1 and CCR2 genes and a significant increase in the expression of SDF-1 Page of (page ... documented role in the local accumulation of monocytes [8,9] However, fenofibrate had an intriguing effect in SDF-1 expression neutralizing the significant decrease in its expression observed in untreated ... significant effect of fenofibrate in the MCP-1/CCL2 gene expression However, there was a significant decrease in the gene expression of the CX3CL1/ Fractalkine in patients treated with fenofibrate with...

Ngày tải lên: 10/08/2014, 05:21

... their role in the pathogenesis of such infections has not yet been extensively studied Of the seven genes examined in this study, the levels of expression of IFN-g, IL-2 and IL12P40 genes in the ... paradigm by examining the transcriptional profile of cytokines in the bursal tissues of chickens infected with either vvIBDV H strain or the cell-adapted virus Ts strain at 1, and days postinfection ... change in expression of IL-10 at dpi was 28.8-fold higher (P = 0.03) In contrast, the expression of IL-5 mRNA in the bursa of birds infected with the H strain increased continuously, peaking at...

Ngày tải lên: 11/08/2014, 21:21

... B-cell line - 14 - Introduction Louckes Injection of BARF1-expressing BALB/c3T3 cells into newborn rats resulted in the induction of aggressive tumours, while injection of BARF1-expressing Louckes ... binding of EBNA1 to oriP which contains multiple EBNA1 binding sites OriP is a cis-acting element in EBV genome By associated with EBNA1, it enables the viral persistence of episomes in EBV infected-cells ... In contrast to the majority of cellular genes, many EBV genes expressed during lytic cycle are intronless, and SM may therefore be important in enhancing expression of other lytic EBV genes In...

Ngày tải lên: 05/10/2015, 22:32

... Expression of prion genes in the developing zebrafish Fig Alignment of the conserved sequence motifs of the C-terminal domain among members of the PrP family Amino acid numbering starts from the initiator ... within a single exon of 2018 bp In mammals, PrPC is also encoded by an intronless ORF [1] The ATG codon was localized 503 Expression of prion genes in the developing zebrafish bp downstream of ... and amino acid positions (Fig 2) indicated that zebrafish PrP1 is, as PrP2, a member of the PrP family Analysis of the N-terminal repeat domain of zebrafish PrP1 and PrP2 The N-terminal domain of...

Ngày tải lên: 19/02/2014, 16:20

... studying the influence of SARS- CoV on the immune system-independent induction of cytokines Interferon genes and their antiviral effectors To properly assess the cytokine profile of SARS-CoV infection, ... production of chemokines by the infected tissue cells Results Virus-induced cytokines not only play a significant role in host defense, but also in immunopathogenesis Investigations of the cytokine profiles ... dissemination of SARS-CoV By slowing down expression of IFNs and their antiviral genes in the infected tissue cells, the virus buys time during the initial, critical phase of infection in order...

Ngày tải lên: 20/06/2014, 01:20

... important regulators of cell apoptosis, it is possible that HBV infection might alter the cIAPs gene expressing of liver cell, finally instigating the carcinogenesis of the cells it infected This is ... mechanism of how these proteins contribute to the carcinogenesis is still in the investigation The involvement of these proteins in the NF-kB related signal transduction pathway that results in the ... the inhibition of active Int J Med Sci 2005 2(1) 34 caspases by the direct binding of IAPs In the many case, for example, in the lung carcinoma cells, the interaction of the overexpressed cIAP2...

Ngày tải lên: 02/11/2012, 11:17

... reflect ammonia oxidizing activity This low amount of amoA mRNA in Run should be related to the fact that 40% of ammonia remained at the end of batch experiment Small decrease in the amount of amoA ... the number of ammonia oxidizer, instead, the amount of amoA mRNA is affected in a short time Because amoA mRNA is one of the key templates of ammonia monooxygenase, copy number of amoA mRNA should ... shows amoA mRNA copy number in a PCR-reaction tube, and the quantity of the sample in each tube was the same Amount of amoA mRNA increased in all runs after starting the experiment The highest amount...

Ngày tải lên: 05/09/2013, 10:15

... eosin staining We examined the expression of these proteins in a cancer-free layer of squamous epithelial cells, carcinoma in situ (CIS) and invasive cancer, all of which were observed in the same ... comparing the amounts of Mcm in total cellular proteins and chromatin-bound proteins, approximately one-third of total Mcm protein was found to be recovered in the chromatin-bound fraction in HeLa ... expression of Mcm genes in growth-arrested cells is induced by addition of serum [43] There are several E2F binding sites in the promoter region of the Mcm5, 6, and genes, and transcription of the genes...

Ngày tải lên: 20/02/2014, 23:20

... control of the GAL10 promoter The induction of the yeast, by galactose containing media, results in overexpression of GAL4, in turn inducing an increase of the expression of the OR gene under control ... function of increasing amount of total proteins in membrane fractions from I7 yeast strain induced at 15 °C, and from yeast expressing SSTR2 as a control activity By taking advantage of structural ... several-fold higher in the strain coexpressing the Golf subunit in comparison to the strain coexpressing the Ga15 subunit when grown in the same conditions 527 Expression of olfactory receptors in yeast...

Ngày tải lên: 07/03/2014, 16:20

... protein metabolism in these cells Histone is rich in the basic amino acids, lysine and arginine, but contains no cysteine Protamine, which is replaced for hitstone via transit proteins during ... proteins during spermiogenesis, is rich in both arginine and cysteine The cysteine sulfhydryls in protamine must form disulfide bond to package DNA into small sperm head during the maturation process ... pumped out of the cells Thus, GR indirectly participates in the protection of cells against oxidative stress The enzymatic activities of GR have been investigated in various tissues under physiologic...

Ngày tải lên: 17/03/2014, 17:20

... diagram of the fusion of cells producing individual protein units of a multisubunit protein Three transfected CHO-K1 cell lines producing either IgA, J-chain or SC, respectively, were fused in ... Simultaneous expression of multi-subunit proteins in mammalian cells using a convenient set of mammalian cell expression vectors Biotechniques 23, 406–407 13 Horlick, R., Schilling, A., Samama, P., Swanson, ... expressed the individual protein subunits by conventional transfection Transfection of the SC gene, the J-chain gene and cotransfection of the a1 heavy-chain and k lightchain genes resulted in clones...

Ngày tải lên: 18/03/2014, 01:20

... enzymes that in uence transcription by selectively deacetylating or acetylating the e-amino groups of lysine located near the amino termini of core histone proteins Acetylation of p65 at lysines 218, ... protein kinase Rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant) Rho-associated, coiled-coil containing protein kinase Ras-related C3 botulinum toxin substrate (rho family, ... factor) Interleukin 1, beta Mitogen-activated protein kinase 14 Mitogen-activated protein kinase 13 Mitogen-activated protein kinase Mitogen-activated protein kinase Mitogen-activated protein kinase...

Ngày tải lên: 22/03/2014, 16:20

... transfer into various dividing and non-dividing target cells They offer several advantages over other vectors, including stable integration into the host cell genome, lack of transfer of viral genes, ... to link two genes transcribed from a single promoter within recombinant viral vectors However, there are a number of limitations using IRES elements, including their size and variability in expression ... different protein products A selfprocessing activity in FMDV leads to 'cleavage' between the terminal glycine of the 2A product and the initial proline of 2B The exact mechanism of 2A/2B cleavage...

Ngày tải lên: 19/06/2014, 08:20

... transfer into various dividing and non-dividing target cells They offer several advantages over other vectors, including stable integration into the host cell genome, lack of transfer of viral genes, ... to link two genes transcribed from a single promoter within recombinant viral vectors However, there are a number of limitations using IRES elements, including their size and variability in expression ... different protein products A selfprocessing activity in FMDV leads to 'cleavage' between the terminal glycine of the 2A product and the initial proline of 2B The exact mechanism of 2A/2B cleavage...

Ngày tải lên: 20/06/2014, 04:20

... representative genes from a variety of different groups of sepsis mediators: inflammatory cytokine interleukin-6 (IL-6), anti-inflammatory cytokine interleukin-10 (IL-10), chemokine interleukin-8 (IL-8), intercellular ... Mediator inflammatory response Chemotactic mainly for neutrophils Interleukin 10 IL-10 Monocytes, lymphocytes Anti-inflammatory, inhibits synthesis various pro-inflammatory cytokines Intercellular ... threshold, to promote and maintain rolling interactions [28] In our study, expression of L-selectin was depressed in the HT group This finding is consistent with previous findings and suggests that...

Ngày tải lên: 20/06/2014, 23:20

... the sequence of CaMV35S promoter which located infront of AtAOS2 gene in pCAMBIA1201/AtAOS binary vector (Fig 3) PCR was carried out according to the PCR Amplification Kit by using HiPi PCR Premix ... according to the PCR Amplification Kit by using HiPi PCR Premix (ELPis Biotech, Korea) in a 20 µl reaction volume, with thermal cycling parameters as follows: initial denaturation at 94 0C for min; ... transgenic rice plants were extracted by using the DNeasy Plant Mini Kit (QIAGEN) according to the instructions of the manufacturer 2.2 PCR analysis 2.2.1 Design of Primers The Primer program was used...

Ngày tải lên: 06/08/2014, 18:21

... to examine the expression of the Tax protein and showed the expression of Tax in synoviocytes in all samples examined Although Tax expression was not correlated with cytokine concentration in SF, ... or definite osteophytes with moderate joint space narrowing; IV, severe with large osteophytes and definite joint space narrowing bScoring was as follows: +/-, minimum staining in one area of the ... tissue; +, patchy staining involving several areas; ++, moderate diffuse staining OST, osteotomy; SYV, synovectomy; TA, total joint arthroplasty Of the cytokines examined in our study, C-PTHrP...

Ngày tải lên: 09/08/2014, 01:23

... regarding the molecules acting upstream of the immune-mediated events that lead to the amplification of the inflammatory cascade IL-18, although capable of inducing Th2 cytokines in an IL4 independent ... epithelial cells, no staining for IL-18 was found in acinar cells (Fig 3a,3d) In a minority of patients with chronic R452 Of the 32 periductal inflammatory foci identified in the samples studied, 19 ... uncoupled from or independent of the level of cellular infiltration In addition, the demonstration of increased ductal expression of IL-18 in SS would be in keeping with other autoimmune conditions...

Ngày tải lên: 09/08/2014, 01:23