α concentration by enzyme linked immunosorbent assay

... goat anti-mouse Conjugated Enzyme ex: goat anti-mouse with HRP Substrate (enzyme conversion) ex: DAB Antigen ELISA for Antibody Detection (Enzyme- Linked Immunosorbent Assay) • • • • • • • • • ... to wells Washs Add 2nd antibody (attach enzyme) Washes Color development Ex: HIV test Sandwich ELISA for Antigen Detection (Enzyme- Linked Immunosorbent Assay) • • • • • • • Capture 1st antibody ... Washes • Color development • Ex: Pregnancy test Enzyme- Mediated Detection Enzyme Horseradish Peroxidase Substrate Abbrev Color Diaminobenzidine DAB Brown Enzyme Alkaline Phosphatase (AP) Substrate...

Ngày tải lên: 15/07/2014, 17:00

... KỸ THUẬT ELISA (ENZYME- LINKED IMMUNOSORBENT ASSAY) TRONG XÁC ĐỊNH CHỦNG MYCOBACTERIA VÀ TRONG CHẨN ĐOÁN SÀNG LỌC BỆNH LAO I NGUYÊN ... nhiễm bệnh thể Phức hợp kháng nguyên-kháng thể nhận biết cộng hợp kháng huyết tương ứng gắn với enzyme peroxidaza phosphataza… gây chuyển mầu chất phù hợp Kết phản ứng đo thiết bị đo quang phổ...

Ngày tải lên: 24/07/2014, 20:22

... a beta density, B (α, β), as suggested by many authors [12, 15, 17, 23] The prior density for each test parameter was selected with the mean of the beta distribution given by α/ α+β, and the standard ... Prevalence in Dairy Cattle in a Province of Korea using an Enzyme- linked Immunosorbent Assay: Application of Bayesian Approach 55 prevalence, as noted by Brenner and Gefeller [4] For sensitivity the resulting ... Estimation of Paratuberculosis Prevalence in Dairy Cattle in a Province of Korea using an Enzyme- linked Immunosorbent Assay: Application of Bayesian Approach 53 the binomial distribution in terms of the...

Ngày tải lên: 07/08/2014, 17:22

... )VDEP( suriv aehrraid cimedipe enicrop no yevrus lacigolores dna 9-VDEPK fo noitatpada lleC HS nA ,BY gnaK ,JB nowK ,HC noewK 31 452-942 ,33 ,3991 seR teV J naeroK aeroK ni )VDEP(suriv aehrraid cimedipe...

Ngày tải lên: 07/08/2014, 18:21

... HIV1 kháng thể anti- HIV2 bất hoạt tác dụng hóa quang học (R4) + Pha loãng mẫu: PBS buffer (R6) + Enzyme trực tiếp chống lại kháng thể IgG người.(R7) + Chất đệm Peroxidase (R8) + Chất nhiễm sắc:...

Ngày tải lên: 05/06/2017, 09:30

... coupled enzyme (Fig 4C) When was used as a substrate, 100 ng of the enzyme was required for determination of the activity by this assay method The addition of 50 mU of coupled enzyme to the assay ... Compound was assayed with A orientalis b-NAHase as a coupled enzyme and 3–5 with almond b-D-glucosidase using the newly developed colorimetric assay described above Compound was assayed by HPLC The ... of the endo-b-galactosidase could be determined by this assay method The dose–response plot of the coupled enzyme vs the colour intensity of the enzyme was found to be a plateau in the range of...

Ngày tải lên: 31/03/2014, 07:20

... Ins(1,4,5)P3 increase by prolyl endopeptidase (Eur J Biochem 269) 5817 Fig Ins(1,4,5)P3 concentrations in various U343 cell lines stimulated by Substance P Ins(1,4,5)P3 concentrations were measured ... cloning rings (Clontech) Prolyl oligopeptidase assays Cells (1 · 107) were harvested by washing twice in NaCl/Pi (Gibco BRL) and resuspended in 200 lL assay buffer (50 mM Hepes, pH 7.5, 200 mM NaCl, ... 3D; BioSciTec, Marburg, Germany) Assay of Ins(1,4,5)P3 Cells were grown in 25 cm2 culture flasks to nearly 100% confluence Ins(1,4,5)P3 concentration was determined by an isotope dilution method (Amersham...

Ngày tải lên: 31/03/2014, 08:20

... transport Results Alteration of intracellular potassium concentrations by recombinant Nef To quantify the effect of Nef on intracellular potassium concentrations ([K+]), RH9 cells from a CD4+ T-lymphoblastoid ... then incubated with various concentrations of recombinant HIV-1 or SIV Nef protein for 15 at 37°C, and fluorescence intensity was measured by using a fluorescence concentration analyzer Each ... ester group linked to each dye renders the molecule uncharged and thereby able to permeate living cell membranes Once inside the cell, the lipophilic blocking groups are cleaved by endogenous...

Ngày tải lên: 20/06/2014, 01:20

... subdomains called α1 , α2 and α3 (Tragardh, et al., 1979) composed of 90, 92 and 92 amino acids, respectively (Malissen, et al., 1982) α1 and α2 domains are distal to the cell membrane and α3 Introduction ... Nterminal α1 and α2 domains fold together to form the peptide-binding groove, which is a platform with an eight-stranded β-pleated sheet topped by two long α helices Each of the α1 and α2 domains ... Introduction Peptide A α2 Domain β2-Microglobulin α3 Domain B α1 Domain α1 Domain α2 Domain Figure 1.4 Ribbon diagram showing the three-dimensional structure of HLA class I molecule The α chain is shown...

Ngày tải lên: 03/10/2015, 20:58

... preservation of enzyme activity The fractions were assayed for thymidine kinase activity under standard assay conditions with 100 lm dThd Thymidine kinase assay Thymidine kinase activity was assayed by measuring ... form, irrespective of the cellular ATP concentration, due to the low enzyme concentration As the TK1 concentration increases during S phase, more and more of the enzyme will be in the tetramer form ... enzyme concentration on the oligomerization status, the elution profile of rhTK1 was analyzed under the conditions and at the concentrations outlined in Fig In Fig 5A, rhTK1 was applied at a concentration...

Ngày tải lên: 18/02/2014, 12:20

... Cys182 and the Cb of ˚ Tyr225 was reduced by % A in WT thrombin by + Na binding and mediated the conformational transitions in the catalytic pocket of the enzyme responsible for the enhanced catalytic ... reducing agent concentration allows the disulfide bonds to scramble and rearrange according to conformational changes induced by the denaturant This allowed us to assess, better than by the simple ... another evidence that the conformational transition caused by Lys9 deletion in the A-chain are sensed by catalytic subsites of the mutant enzyme These findings lead us to propose that a significant...

Ngày tải lên: 07/03/2014, 12:20

... zonal electrophoresis [24] Competition assay The ACE competition assay is based on the ACE activity assay using a simple radio assay for angiotensin-converting enzyme [14,15,25] Briefly, ACE-activity ... buffer (pH 8.5) at a concentration of 35 mgÆL)1 and was allowed to refold by air oxidation for 17 h at room temperature under constant stirring The refolded peptide was purified by semi preparative ... to ACE has been observed because of the enzyme elimination by acidic precipitation before the centrifugation The peak area is proportionnal to the peptide concentration as shown in Fig In order...

Ngày tải lên: 23/03/2014, 12:20

... hormone FSH: Follicle Stimulating hormone GnRH: Gonadotropin Releasing hormone ELISA: Enzyme Linked Immuno Sorbent Assay FBS: Fetal Bovine Serum FRH: Folliculin Releasing hormone HCG: Human Chorionic ... phòng thí nghi m Vi t Nam hi n ñang dùng phương pháp ch n ñoán mi n d ch enzym ELISA (Enzyme linked immuno sorbent assay) có ñ xác cao, có th nhanh chóng xác ñ nh ñư c n ng ñ FSH, LH máu s a s ñó ... không ch a Do PGF 2α t nhiên b phân hu r t nhanh giá thành s n xu t cao nhà s n xu t ñã làm ch ph m có tác d ng tương t PGF 2α t nhiên có tác d ng m nh hơn, th i gian phân hu lâu PGF 2α t nhiên Có r...

Ngày tải lên: 08/08/2013, 20:56

... experiment, the initial concentration of cadmium was set to 1.0 mg/L and after 55 days, the cadmium concentration was increased to 5.0 mg/L by extra addition experiment to clarify the concentration of ... Analyses The concentrations of water with dissolved cadmium and phosphorus were determined using a spectrophotometer (pHotoFlex, WTW, Germany) The concentration of GSH was measured by colorimetric ... with HCl, and the concentration of cadmium was measured by the method mentioned previously RESULTS AND DISCUSSION Removal of cadmium and phosphorus Time courses of cadmium concentrations in culture...

Ngày tải lên: 05/09/2013, 10:17

... enzyme [25] Attempts to so were hampered by the experimental requirement for high enzyme concentrations both in terms of estimating the true free-ligand concentration in the experiments and by ... the mutant enzymes H121A and V122G was inspired by the results from analysis of crystal structures as discussed later Both mutant enzymes were produced in similar amounts as wild-type enzyme and ... similar from a regulatory point of view dCMP deaminase is activated by dCTP and inhibited by dTTP and both nucleotides act on the enzyme by binding to an allosteric site to alter the cooperativity of...

Ngày tải lên: 18/02/2014, 16:20

... (Invitrogen) However, in vitro assay of the cell-free extracts of these transformants with 14C-labeled b-amyrin as a substrate did not show any hydroxylase activity, and in vivo assay by feeding the same ... at the C-22 position is catalyzed by other P450, probably an enzyme similar to CYP93E1 A similar metabolic grid branching at the hydroxylation of campestanol by two independent P450 (6-hydroxylase ... apoptosis by mitochondrial perturbation Proc Natl Acad Sci USA 98, 5821–5826 Ko K-S, Noguchi H, Ebizuka Y & Sankawa U (1989) Oligoside production by hairy root cultures transformed by Ri plasmids...

Ngày tải lên: 19/02/2014, 07:20

... absorption spectra in a coupled enzyme assay, the enzyme reaction product identified by GC-MS analysis, and the determination of released ammonia The coupled enzyme assay revealed the mechanism of ... were pooled (fraction 6, 24.5 mL) The enzyme purity was checked by SDS/PAGE [16] Production and isolation of enzymatic reaction products in a coupled enzyme assay The reaction mixture contained ... of the native enzyme was determined by gel filtration on Cellulofine GCL-1000 sf using the method reported previously [10] The molecular mass of the enzyme subunit was determined by SDS/PAGE [16]...

Ngày tải lên: 19/02/2014, 16:20

... assays Fluorogenic assays using the synthetic ACE2 substrate, Mca-APK(Dnp) [4] (final concentration 25 lm) were carried out at room temperature The assay was monitored continuously by measuring the ... independent determinations APK(Dnp) by ACE2 is greatly enhanced in the presence of chloride ions [4,17] At high concentrations of NaCl (0.5 m), the activity of the wild-type enzyme was increased  3.5-fold ... Angiotensinconverting enzyme is an essential regulator of heart function Nature 417, 822–828 Allred AJ, Donoghue M, Acton S & Coffman TM (2002) Regulation of blood pressure by the angiotensin converting enzyme...

Ngày tải lên: 20/02/2014, 01:20

... protein extracts from Arabidopsis leaves By employing the citrulline-based NOS assay, an arginine-dependent activity was discovered that was strongly stimulated by an extract of low molecular weight ... inhibition of the activity at concentrations of diphenylene iodonium up to 10 lm (data not shown) Second, the products of the reaction were analyzed by one-dimensional TLC followed by autoradiography No ... fumarate by argininosuccinate lyase (ASL; EC 4.3.2.1; Fig 5) Argininosuccinate is normally made from citrulline and aspartate by argininosuccinate synthetase, but it can also be produced by ASL...

Ngày tải lên: 07/03/2014, 05:20

... Glucose was determined either by glucose test strips (Roche), by HPLC analysis or enzymatically essentially as described by Bergmeyer [42] Nitrate was detected by nitrate ⁄ nitrite test strips ... HCl and detection by refractive index and UV at 210 nm Extracellular polyols and acids were calculated as concentration measured extracellularly (molÆL)1) divided by dry biomass concentration (g ... were also as described by Ruijter and Visser [41] The assay for 6PG was the same as for G6P, except that G6PDH was exchanged with 6PGDH The assay for NADP was as described by Klingenberg [47] with...

Ngày tải lên: 07/03/2014, 17:20