• the sky is falling novel by sidney sheldon

... Poor devils. They think this is some kind of heaven. They tell me this is easy work compared to the type they had to undergo. The Satheri like to get big bunches through in one conjuration, like the haul ... labor gangs. The idiots must be trying to reach the sky with their pyramid. There could be no other answer to the immense bulk planned for this structure. Like the pride-maddened men of Babel, they were ... rushed upwards. Another began to rise, and another. In seconds, more than half of those who had waited were screaming upwards toward the hole in the sky. They disappeared in the distance. Those who...

Ngày tải lên: 15/03/2014, 17:20

... site-directed mutagenesis of Cys153. Mutation of Cys149 was not attempted, because this residue is known to be abso- lutely essential for catalysis [20]. On the other hand, the activity of the purified recombinant ... significant role in the protection of A n B n GAPDH isoforms against oxidative stress. Discussion The aims of the present study were to establish whe- ther chloroplastic GAPDH isoforms undergo gluta- thionylation ... in higher plants displays the A n B n structure and is regula- ted by metabolites and thioredoxin f (TRX f), a small disulfide oxidoreductase involved in the activation of the Calvin cycle by light [29–33]....

Ngày tải lên: 19/02/2014, 05:20

... have not been entirely encompassed by the drug-treatment. Such a hypo- thesis is further confirmed by the observation that, differ- ently from the effect of serum, the stimulatory effect of PMA on ... the molecular mechanisms by which this novel ruthenium complex exerts its antimetastatic activity are largely unknown. In this study, we attempted at elucidating the molecular target(s) and the ... counteracting DNA synthesis over a 1–200 l M range (Fig. 1B). Further analysis of the inhibitory response elicited by NAMI-A on ECV304 growth was accomplished by inves- tigating the effect of NAMI-A...

Ngày tải lên: 21/02/2014, 01:21

... fashion do, with the ability to choose clothes that manage to work so perfectly that they count twice––once for how they look and then again because they proclaim to the world: this lady knows what’s ... Muzak. Then, though, a pianist takes the stage, and can you believe this: he plays only with his left hand. First Ravel, then Strauss. Who would believe this? Who would have known that there are ... know, like in the Olympics where they throw that Frisbee-type thing. The famous sculpture.” The discus thrower,” one of the more learned writ- ers said. “Right. I’m tellin’ ya, if the sonuvabitch...

Ngày tải lên: 21/02/2014, 06:20

... based on the assumption that the transient interaction between the enzyme and its substrate is strengthened if the course of the catalytic reaction is hampered. In accor- dance with this, the low ... 1B). The partial phosphorylation of Grx4p still observed in the case of the two mutants (corresponding to 30–40% of that of the wild-type) could be explained either by the intervention of another ... contributes to the full functionality of Grx4p in vivo.We also show that this regulatory system is influenced by the phosphorylation of Bud32p at Ser258, which is specifically mediated by the Sch9p...

Ngày tải lên: 07/03/2014, 04:20

... participate in the translational enhancement mediated by introns. Indeed, the presence of the PAP Gld2 on the messen- ger, targeted by a protein of the Rbm9 family, may allow the polyadenylation of the ... mammalian Rbm9 is unclear and is dependent on the isoform and the tissue examined; however, it appears to be mainly nuclear in cell lines and brain where, nevertheless, there is addi- tional ... oocytes. The antibodies, by interacting with their target proteins, could disrupt this masking complex, alleviate the repression and allow the translation of maturation- required proteins before the...

Ngày tải lên: 07/03/2014, 05:20

... his3:hisG/his3::hisG trp1::hisG/trp1::hisG This study MR211 S1278b; MATa ura3–52 his3:hisG leu2::hisG ras2::kanMX MR298 S1278b; MATa/a ras2::kanMX/ras2::kanMX ura3–52/ura3–52 his3:hisG/his3:hisG leu2::hisG ... his3:hisG/his3:hisG leu2::hisG leu)2::hisG This study AR2 S1278b; MATa/a sut2::S.pombe his5 + /sut2::S.pombe his5 + ura3–52/ura3–52 his3:hisG/his3:hisG leu2::hisG/LEU2 TRP1/trp1::hisG This study MR287 S1278b; ... ura3–52 his3:hisG leu2::hisG FLO11::lacZ This study AR3 S1278b; MATa ura3–52 his3:hisG leu2::hisG ras2::kanMX FLO11::lacZ This study AR4 S1278b; MATa ura3–52 his3:hisG leu2::hisG sut2::S.pombe his5 + FLO11::lacZ...

Ngày tải lên: 07/03/2014, 15:20

... kinases was induced by leptin. The weak response of STAT5 to leptin compared to that elicited by GH can be partially explained by the rather ineffec- tive transfection of the HIT-T15 cells (estimated ... lg each of the luciferase reporter construct and the b-galactosidase reporter control plasmid pSVb-gal (Promega). Transcription of the lacZ gene in this control vector is driven by the SV40 early ... analysis are consistent with findings obtained with other cytokine receptors: the position after the phosphotyrosine (P +1) is critical for binding of STAT5 but not of STAT1 or STAT3, whereas the glutamine...

Ngày tải lên: 07/03/2014, 16:20

... mutagenesis of the N-terminus of the V 1a R; this revealed that a single residue (Arg46) located in the distal segment of this domain is critical for high affinity agonist binding but not antag- onist ... than the wildtype V 1a R but once the agonist bound, the receptor was more likely to signal. This dual role suggests that binding of the agonist releases the Arg46-mediated constraint on the ground ... rescued by a chimeric construct in which the N-terminus of the OTR is replaced with the corresponding sequence of the V 1a R [10]. The role of the N-terminus has been addressed recently by alanine-scanning...

Ngày tải lên: 07/03/2014, 21:20

... of the regula- tion of the Muc5b promoter by TTF-1 and GATA factors. Using this approach, we aimed to show the transcriptional regulation of Muc5b by these two tran- scription factors, thereby ... pathophysiology. The analysis of the promoter sequences of the murine Muc5b and human MUC5B genes showed that they are highly similar over the first 170 nucleo- tides and, more importantly, that the TATA box is identical. ... in the primitive gut [8]. TTF-1 and GATA-6 are required for the formation and differentiation of distal epithelium [20,44,45]. In normal adult tissue, MUC4 is preferentially expressed by the...

Ngày tải lên: 15/03/2014, 00:20

... with the sweetness of the midsummer night, and the By the Light of the Soul 13 “No sicker than usual,” replied her mother. Then she drew the delicate little figure close to her, and kissed ... said her father. Maria looked at her father in a bewildered sort of way. “I guess the coffee is in the other canister,” said she, meekly. “Why didn’t you say so then?” demanded her father. Maria ... coffee at the price it is now? I guess she doesn’t,” said her father. He poured the coffee-pot full of boiling water from the tea-kettle, then he tipped the coffee canister into his hand, and...

Ngày tải lên: 16/03/2014, 18:20

... binding site. There is no typical TATA or CAAT box in the putative promoter region. The putative signal sequence is encoded by the first exon, and the pro-enzyme frag- ment is encoded by exons 1 and 2. The ... prosemin is expressed strongly in the pancreas, but weakly in the prostate and cerebellum (Fig. 4). The nucleotide sequences of the cDNA and the cosmid clone are identical. A comparison of the cDNA ... [29]. Northern blot analysis showed that, of the normal tissues, prosemin is abundantly expressed in the pancreas (Fig. 4). All the pancreatic cancer cell lines tested secreted prosemin into the con- ditioned...

Ngày tải lên: 16/03/2014, 23:20

... 2002 determination of k diss by the method used for papain, in which the displacement was monitored by chromatography. Therefore, only upper limits of k diss for the binding of these mutants to cathepsin L ... agreement with the calculated k diss (Table 2). The L73G mutation markedly affected the rate of dissociation of the complex with cathepsin B, increasing k diss by  8000-fold. The P74G mutation ... are formed. This conclusion is indicated by the L73G mutation appreciably decreasing the affinity of cystatin A for the proteases by increasing the rate constants for dissociation of the complexes...

Ngày tải lên: 17/03/2014, 10:20

Ngày tải lên: 23/03/2014, 07:20

Ngày tải lên: 23/03/2014, 12:20

Ngày tải lên: 24/03/2014, 03:21