ligand protein interactions of glutamate receptor
Báo cáo y học: "Protein-protein interactions of the hyperthermophilic archaeon Pyrococcus horikoshii OT3" pot
... functions of the uncharacterized proteins In uncovering the functions of proteins, systematic examination of protein- protein interactions (PPIs) is important Because most proteins operate as parts of ... - Observation of Pyrococcus protein- protein interactions in vitro and the Figure heat pre-incubation on synthesized proteins effect of3 Observation of Pyrococcus protein- protein interactions in ... of the hetero -interactions between proteins of the same class consisted of the archaea-specific protein pairs (17 out of 30 interactions) , and in the interactions between proteins of different...
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The investigation of transcription related protein protein interactions of histones in saccharomyces cerevisiae
... detection of protein- protein interactions occurring anywhere inside the cell and is suitable to screen transactive baits Ubiquitin is a small protein important for the turnover of cellular proteins Proteins ... levels of cellular function The presence of protein interaction networks is a key property of complex biology systems A large number of methods have been developed for screening protein interactions ... pull down assay, allow the study of 26 physical interactions of proteins, but pull down assays require a certain stability of the protein complex Moreover, many of these methods are relatively...
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The study of interactions of transmembrane receptors and intracellular signaling proteins in live cells by fluorescence correlation and cross correlation spectroscopy
... Table of Contents Acknowledgements i Table of contents ii Summary v List of Tables viii List of Figures ix List of Symbols xi Chapter Introduction 1.1 Methods to detect protein- protein interactions ... two groups of proteins, the whole study may reveal SWFCCS as a versatile technique in detecting protein- protein interactions in live cells The activation mechanism of ErbB receptors is of fundamental ... quantification of complex (dimer) percentages of interacting proteins and the determination of the equilibrium dissociation v constants of binding proteins The experimental setups of FCS/SW-FCCS...
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Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf
... D2 receptors and their interactions with the appropriate a subunits of G protein, further confirm that the use of advanced fluorescence techniques does indeed allow for the observation of true interactions ... the C-terminal end of the D1 receptor, as well as the Arg-rich region of ic3 of the D2 receptor, not seem to take part in receptor homodimerization, but they influence D1–D2 receptor heterodimerization ... the C-tail of the D1 receptor The efficiency of energy transfer was reduced to 0.8% A possible interpretation of the data suggests that the indicated basic region of ic3 of the D2 receptor and...
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Báo cáo khoa học: Insights into the activation of brain serine racemase by the multi-PDZ domain glutamate receptor interacting protein, divalent cations and ATP ppt
... means of the yeast two-hybrid approach, three proteins have been shown to interact with brain SR: glutamate receptor interacting protein (GRIP) [1,12], PICK1 [13] and Golga3 [14] GRIP is a large protein ... GRIP is a very large protein (1112 amino acids) composed of at least nine protein modules The architecture of this protein is depicted in Fig 2A Each PDZ domain is comprised of approximately 100 ... isolated PDZ6 domain of GRIP (residues 665–768) Both recombinant proteins were purified to homogeneity (Fig 1B) Because the PDZ6 domain of GRIP is just a part of the entire protein, we confirmed...
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Development of a novel toll like receptor based two hybrid assay for detecting protein protein interactions and its application in the study of CD14 dimerization and FcyRIIA activation
... mechanism can be utilized to detect proteinprotein interactions 99 3.2 Expression of TIR1 and TIR2 fusion proteins 101 3.3 Detection of ligand-induced receptor- receptor interaction using the TIR1/TIR2-based ... Detection of specific IL-4 interactions with the IL-4Rα but not γC 105 3.5 Detection of homotypic interactions between IL-4Rα and γC receptors 108 3.6 Detection of interactions between secreted proteins ... blocking of any of the three FcγRs 152 5.7 Conclusion 156 Chapter 6.1 Discussion Development of a TLR-based two-hybrid assay for the detection of proteinprotein interactions 6.2 158 Investigation of...
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Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc
... EI (enzyme I of the PTS), the rst protein in the cascade of proteins forming the PTS, to HPr, the histidine-phosphocarrier protein HPr is the smallest protein in the protein cascade of the PTS ... stability of a protein In Protein Structure (Creighton, T.E., ed.), 2nd edn, pp 253259 Oxford University Press, Oxford 35 Privalov, P.L (1979) Stability of proteins: small globular proteins Adv Protein ... presence of urea Biochemistry 34, 67956804 53 Privalov, P.L & Makhatadze, G.I (1990) Heat capacity of proteins II Partial molar heat capacity of the unfolded polypeptide chain of proteins: protein...
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Báo cáo khoa học: A novel mechanism of V-type zinc inhibition of glutamate dehydrogenase results from disruption of subunit interactions necessary for efficient catalysis doc
... given in Table Effects of zinc on cofactor binding The effects of zinc on the binding of the reduced cofactor NADPH to the enzyme, at pH 8.0, in the presence or absence of glutamate were examined ... homotropic interactions as a possible explanation of coenzyme activation of glutamate dehydrogenase FEBS Lett 1, 349–352 Engel PC & Dalziel K (1969) Kinetic studies of glutamate dehydrogenase with glutamate ... activity of the enzyme This observation has been extended to include a number of other metal ions Our observations of the effects of decreasing glutamate concentrations on the apparent affinity of the...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... for elucidating protein protein interactions and selecting specific binders to novel target proteins, and the first and second minireviews focus on the detection of protein protein interactions [8,9] ... scaffold proteins, and as methods for designing high-quality libraries of small scaffold proteins Functionalization of small scaffold proteins by peptide grafting The design of chimeric proteins, ... Functionalization of small scaffold proteins by replacing a loop of the scaffold protein with a CDR loop of antibody fragments (A) Replacement of the candidate location in NCS for grafting with the CDR loop of...
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Báo cáo khoa học: The capsid protein of human immunodeficiency virus: interactions of HIV-1 capsid with host protein factors ppt
... Proteomics of HIV-1 capsid A Fig Role of TRIM5a and homology model of its PRYSPRY domain (A) The hypothesized role of TRIM5a in the HIV-1 viral life cycle (B) A homology model of the PRYSPRY domain of ... a broad array of viruses, including N-MLV, HIV-1 and HIV-2, were found to be species-specific variants of TRIM5a [57–59] TRIM5a is a member of a large family of tripartite motif proteins with ... synthesis of viral DNA using host cell tRNALys as a primer for initiation Synthesis of cDNA initiates from a primer binding site of 18 bases near the 5¢ end of viral genomic RNA The 3¢ terminal end of...
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Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx
... Kinetic models of multisite phosphorylation C Salazar and T Hofer ¨ A B Fig Plasticity of the stimulus sensitivity depends on protein protein interactions Contour plots are given of the effective ... those of their substrates Protein protein interactions Multisite phosphorylation per se is not associated with switch-like responses [18,48] We showed here that the characteristics of protein protein ... fraction of rate of kinase kinaseÀbound target protein P=Qn bn ¼ bn |{z} þ K=Ln þ P=Qn catalytic rate |fflfflfflfflfflfflfflfflfflfflfflfflfflffl{zfflfflfflfflfflfflfflfflfflfflfflfflfflffl} fraction of of phosphatase phosphataseÀbound target protein...
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Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf
... concentrations of NaCl, the fluor3888 escence intensities of GST–HIPPI increased, indicating a lesser extent of interactions of GST–HIPPI with AA AGACATG This result indicated that the interaction of GST–HIPPI ... the intrinsic fluorescence of GST–HIPPI protein, indicating binding of the protein with this mutated motif (Fig 1B, panel II) The apparent dissociation constant (Kd) of this binding was nm (Fig ... presence of glutathione S-transferase (GST)–HIPPI (Fig 1A, panel I, lane 3) indicated interaction of the purified protein with the motif No shift was observed in the presence of GST protein only...
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Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx
... characterized of these is a family of three structurally related, but genetically distinct, 160 kDa proteins called the NR co-activators or p160 co-activators [12–18] These three proteins are steroid receptor ... the functional activities of GRIP1 They extend our understanding of the importance of the structural status of GRIP1 in modulating NR functions Results Autoregulation of GRIP1 AD activities by ... relative concentration of GRIP1 might depend on its homo-oligomerization status, which is mainly deter2180 mined by the involvement of its C-terminal region in protein protein interactions, including...
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Báo cáo Y học: Probing intermolecular protein–protein interactions in the calcium-sensing receptor homodimer using bioluminescence resonance energy transfer (BRET) potx
... the result of specific protein protein interactions between the receptors, rather than nonspecific diffusive lateral motion or clustering of overexpressed receptors First, the lifetime of an excited ... repeatedly in studies of the oligomerization of GPCRs and other protein protein interactions [34–40] In these studies, BRET has been measured using Rluc and enhanced yellow fluorescent protein (EYFP) ... represent a small step forward in the study of protein protein interactions ACKNOWLEDGEMENTS Søren G F Rasmussen and Professor Ulrik Gether are thanked for the use of the SPEX Fluoromax-2 spectrofluorometer,...
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Báo cáo Y học: Interaction of the anterior fat body protein with the hexamerin receptor in the blowfly Calliphora vicina pot
... tissue for studies of the stage-specific and tissue-specific expression of genes, posttranscriptional regulation of RNA, and post-translational control of protein biosynthesis One of the most detailed ... most detailed investigations of fat body proteins has been the metabolism of the storage protein arylphorin, which belongs to the class of hexamerins [5–9] These proteins are synthesized in a ... speculate that, because of the interaction with AFP, most of the hexamerin receptor is inactivated in the anterior fat body preventing uptake of storage protein in this part of the tissue This study...
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Báo cáo khoa học: Construction of a novel detection system for protein–protein interactions using yeast G-protein signaling pdf
... study of protein protein interactions Curr Biol 8, 1121–1124 Ehrhard KN, Jacoby JJ, Fu XY, Jahn R & Dohlman HG (2000) Use of G -protein fusions to monitor integral membrane protein protein interactions ... establish a rapid and reliable method for the detection of protein protein interactions using yeast G -protein signaling In our system, protein protein interactions were detected utilizing the knowledge ... detection of protein protein interactions Detection of interactions in the absence of FEBS Journal 276 (2009) 2636–2644 ª 2009 The Authors Journal compilation ª 2009 FEBS 2639 Detection system for protein protein...
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Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
... environment of the cytosol The primary mechanism for protein degradation of misfolded proteins is the ubiquitin–proteasome system which governs the quality control of proteins Misfolded proteins ... bind to the target 6132 proteins on the lumen side and inhibit transport of target proteins in the process of functional maturation [6,8] If the targets are cytosolic proteins, scFvs without ... 28.8 T7-WASP15 22 Fig Expression of anti-WASP scFvs and detection of their binding activity to WASP in T cells (A) Western blot analysis of protein extracts of anti-WASP scFv DNA-transfected...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
... the ratio of activities determined in the presence and absence of R1881 (C) Western analysis of indicated GalAD-AR.NTD proteins in the yeast protein interaction system (left panel) and of indicated ... specific role of these motifs in AR function [44–47] The increasing number of proteins found to interact with the AR LBD raises the question of the physiological relevance of the many interactions ... function of AR NTD and thus does not allow discrimination between loss of AR.NTD-AR.LBD binding and loss of AR.NTD transactivating function In the yeast assay, loss of transactivation function of AR...
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Báo cáo Y học: Transcriptional activity of interferon regulatory factor (IRF)-3 depends on multiple protein–protein interactions pdf
... corresponding regions of p300 was approximately 2–3 times weaker When binding of IRF-3 to smaller domains of the N- and C-terminal regions of p300/CBP was examined, most of the activity was found ... presence of GST-CBP-C2 (lanes 18, 21, 24 and 27) These interactions were also probed in the absence of DNA using pull-down assays (Fig 5B) Binding of IRF-3 WT to the N- and C-regions of p300 and ... to that of other IRF-3 Ó FEBS 2002 Mechanism of IRF-3 virus-dependent activation (Eur J Biochem 269) 6147 Fig Two domains of IRF-3 are involved in interactions with coactivators (A) Proteins...
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Báo cáo Y học: Ligand-induced heterodimerization between the ligand binding domains of the Drosophila ecdysteroid receptor and ultraspiracle docx
... EcR and USP LBD are capable of dimerization in the absence of a bipartite EcRE, and that this protein protein interaction is dramatically enhanced by the presence of ecdysteroids MATERIALS AND ... Clone pGAD424-USP(172–508) encodes a fusion protein consisting of the activation domain of GAL4 and the C-terminal part of USP including a portion of the hinge region and the LBD It was produced ... the absence of ligand between EcR and USP fragments requires neither the hinge region nor the F domain of EcR, and even helices 11 and 12 of its LBD are dispensible for a protein protein interaction...
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