Experiment 1 Calibration of Volumetric Glassware, FUNDAMENTALS OF ANALYTICAL CHEMISTRYREPORT, Fundamentals of Analytical Chemistry, The calibration and standardization technique is an essential component of all analyticalprocedures. The calibration establishes the link between the analytes response and the analyteconcentration. Typically, chemical criteria are used to determine this connection. If pure reagentsare available, the standards can be created from them or standardized using conventionalquantitative procedures. Most frequently, the standards employed are created outside of theanalyte solution.
International University, Vietnam National University - HCMC Fundamentals of Analytical Chemistry FUNDAMENTALS OF ANALYTICAL CHEMISTRY REPORT EXPERIMENT 1: Calibration of Volumetric Glassware Group: Group members: Section: Châu Hoàn Nhân BTBCIU19055 Lê Thị Hoài Thương BTBCIU19066 Nguyễn Thị Thùy Trang BTBCIU19068 Nguyễn Bình Duy Anh BTBCIU19031 Nguyễn Hồng Dung BTBCIU19002 Huỳnh Bảo Ngân BTBCIU19051 Nguyễn Ngọc Phương Anh BTBCIU19034 Phùng Thị Ngọc Anh BTBCIU19032 Nguyễn Ngọc Khuê Tú BTBCIU19025 Date: 23/11/2021 Score: International University, Vietnam National University - HCMC Fundamentals of Analytical Chemistry I/ Introduction The calibration and standardization technique is an essential component of all analytical procedures The calibration establishes the link between the analyte's response and the analyte concentration Typically, chemical criteria are used to determine this connection If pure reagents are available, the standards can be created from them or standardized using conventional quantitative procedures Most frequently, the standards employed are created outside of the analyte solution Glassware is often calibrated with known specific gravity liquids and analytical balances The approach is to determine the volume of liquid that the glassware will contain and then divide that amount by the density of the liquid to obtain the equivalent liquid volume Temperature has an effect on density, thus it is required to test the liquid temperature and search up the suitable density numbers The ability to obtain the best data possible from their equipment is a crucial feature of a successful analyst You should calibrate your own volumetric glassware (burette, pipette, flask, etc.) for this purpose in order to quantify the precise volume provided or contained This experiment also encourages enhanced technology in volumetric glassware handling II/ Objective Temperature changes cause the glassware to contract or expand As a result, volumetric glassware calibration is crucial This lab session discusses the necessity of calibrating volumetric equipment, how to identify different types of volumetric glassware, as well as how to calibrate volumetric glassware, and use volumetric glassware correctly III/ Equipment and Chemicals 50mL buret International University, Vietnam National University - HCMC Fundamentals of Analytical Chemistry 100mL flask fitted with a rubber stopper 125mL flask fitted with a rubber stopper 10mL volumetric pipette Micropipette Distilled water IV/ Experimental procedure: data and observation Calibration of buret At the beginning Buret and mass at 10ml point International University, Vietnam National University - HCMC Fundamentals of Analytical Chemistry Buret and mass at 20ml point International University, Vietnam National University - HCMC Fundamentals of Analytical Chemistry Buret and mass at 30ml point International University, Vietnam National University - HCMC Fundamentals of Analytical Chemistry Buret and mass at 40ml point International University, Vietnam National University - HCMC Fundamentals of Analytical Chemistry Buret and mass at 50ml point Volume interval (mL) Difference (mL) Mass delivered (g) Actual volume delivered (mL) Correction (mL) – 10.00 10.0 10.32 10.36 +0.36 10.00 – 20.00 10.00 10.45 10.49 +0.49 20.00 – 30.10 10.10 10.38 10.42 +0.32 30.10 – 40.10 10.00 10.40 10.44 +0.44 40.10 – 50.10 10.00 10.31 10.35 +0.35 International University, Vietnam National University - HCMC Fundamentals of Analytical Chemistry Sum 50.10 mL Sum 51.86 g Average correction +0.392 The total volume of water delivered is (51.86 g) x (1.003 mL/g) = 52.06 mL Because the indicated volume is 50.10 mL, the buret correction at 52.06 mL is –1.96 mL Compare the result from group Volume interval (mL) Difference (mL) Mass delivered (g) Actual volume delivered (mL) Correction (mL) 0.10 – 9.90 09.80 10.22 10.24 +0.44 9.90 – 20.00 10.10 10.44 10.46 +0.36 20.00 – 30.00 10.00 10.39 10.41 +0.41 30.00 – 40.10 10.10 10.40 10.42 +0.32 40.10 – 50.10 10.00 10.31 10.33 +0.33 Sum 50.00 mL Sum 51.76 g Average correction +0.37 The total volume of water delivered is (51.76 g) x (1.003 mL/g) = 51.96 mL Because the indicated volume is 50.00 mL, the buret correction at 51.96 mL is –1.96 mL Calibration of micropipette International University, Vietnam National University - HCMC Fundamentals of Analytical Chemistry Buret and mass at µL, 200 µL, 400 µL and 600 µL point, respectively Volume interval (µL) Difference (µL) Mass delivered (g) Actual volume delivered (µL) Correction (µL) – 200 200 0.2032 203.97 +3.97 200 – 400 200 0.2027 203.47 +3.47 400 – 600 200 0.2026 203.37 +3.37 Sum Sum 600 µL 0.6085 g Calibration of volumetric flask (100ml) Average correction +3.6 International University, Vietnam National University - HCMC 10 Fundamentals of Analytical Chemistry Mass of 100mL flask without water International University, Vietnam National University - HCMC 11 Fundamentals of Analytical Chemistry Mass of 100mL flask with water for the first time Mass of 100mL flask with water for the second time International University, Vietnam National University - HCMC 12 Fundamentals of Analytical Chemistry The first time The second time Difference 164.1130 g 164.0539 g 0.0591 g 164.7366 mL 164.6773 mL 0.0593 mL Compared with the table tolerances listed in the textbook, the tolerances of flask capacity 100mL is ±0.08 We can conclude that the result (+0.0593) is smaller than the theory Calibration of pipet 10ml Mass of 50mL flask without water International University, Vietnam National University - HCMC 13 Fundamentals of Analytical Chemistry Mass of 50mL flask with first 10mL water International University, Vietnam National University - HCMC 14 Fundamentals of Analytical Chemistry Mass of 50mL flask with next 10mL water International University, Vietnam National University - HCMC 15 Fundamentals of Analytical Chemistry Mass of 50mL flask with last 10mL water International University, Vietnam National University - HCMC 16 Fundamentals of Analytical Chemistry Volume (mL) Mass delivered (g) Actual volume delivered (mL) Correction (mL) 10 mL 10.36 10.40 +0.4 10 mL 10.31 10.35 +0.35 10 mL 10.37 10.41 +0.41 Sum 30 mL Sum 31.04 g Average correction +0.39 The total volume of water delivered is (31.04 g) x (1.0038) = 31.16 mL Because the indicated volume is 30 mL, the pipet correction at 31.16 mL is -1.16 mL V/ Conclusion As we all know, all analytical processes require the use of the calibration and standardization technique The calibration establishes the relationship between the analytes’ response and the concentration of the analyte Glassware is frequently calibrated using analytical balances and liquids with specified specific gravity, by dividing the volume of liquid that will be contained in the glassware by the density of the liquid Because density is affected by temperature, it is necessary to test the liquid temperature and look up the appropriate density numbers In this lab experiment we learned how to calibrate the liquid accurately and precisely by using volumetric glassware After completing the trials, there is a difference in the data for each of the calibrations of volumetric glassware International University, Vietnam National University - HCMC 17 Fundamentals of Analytical Chemistry In short, the sensitivity of the instruments affects the accuracy of the volumetric glassware To eliminate parallax error, we must ensure that the eye position is perpendicular to the reading scale of the apparatus Aside from that, the beaker should be well cleaned and dried so that no water remains in it, which could alter the mass of the next trial Moreover, the volumetric glassware must be correctly weighed in order to provide accurate and precise findings ... the necessity of calibrating volumetric equipment, how to identify different types of volumetric glassware, as well as how to calibrate volumetric glassware, and use volumetric glassware correctly... experiment also encourages enhanced technology in volumetric glassware handling II/ Objective Temperature changes cause the glassware to contract or expand As a result, volumetric glassware calibration. .. created outside of the analyte solution Glassware is often calibrated with known specific gravity liquids and analytical balances The approach is to determine the volume of liquid that the glassware