Vài điều về CrisprCas9 (tiếng anh)

CRISPR-CAS9 01 What is CRISPR/Cas9? 02 How CRISPR/Cas9 Work? 03 Applications of CRISPR/Cas9 04 Future of CRISPR/Cas9 History of CRISSPR/Cas9 The CRISPR defense system is CRISPR was called "breakthrough" first identified in prokaryotes scientific research 2000 CRISPRs were described Japanese researchers 2020 2013 1980s 2015 The CRISPR/Cas system can be used for mammalian cell CRISPR method has been awarded the Nobel Prize What is CRISPR/Cas9? “ Molecular scissors ” Components of the CRISPR/Cas9 System Cas9 - gRNA An enzyme The primary function is cleaving A nuclease which targets DNA gRNA - gRNA = crRNA + tracrRNA Cas9 - ‘guides’ Cas9 to the right part of the genome The CRISPR/Cas9 editing tool works gRNA finds the spots and guide the Cas9 to a target Cas9 cuts the target DNA The cut is repaired Diagram showing how the CRISPR-Cas9 editing tool works Image credit: Genome Research Limited Comparison of CRISPR/Cas9 with other Genome Engineering Technologies Zinc Finger Nuclease (ZFN) 04 Transcription Activator-like effector-based nuclease (TALEN) 05 How CRISPR is different from other gene editing tools? Meganuclease 06 Advantage Need attention? of the method -unintended Accurate, efficient, mutations fast and cheapest - Ethical concern Can change the specificity  Can target multiples genes Applications of CRISPR/Cas9 CRISPR Use in Agricultural Sector Use of CRISPR in Cancer CRISPR/Cas9 for Gene-editing CRISPR as Antibiotic Resistance Solution CRISPR/Cas9 has the potential and can be used in various fields, from Medicines to agriculture in the controlled environment CRISPR for AIDS CRISPR Gene Drive What’s the future of CRISPR-Cas9? There are several problems associated with CRISPR/Cas9, like ethical issues and off-target mutations Once these issues are solved, CRISPR/Cas9 will genuinely become a revolutionary medication Reference 1.https://www.yourgenome.org/facts/what-is-crispr-cas9 2.https://nuclineers.com/whats-crisprcas9/#Comparison_of_CRISPR_Cas9_with_other_Genome_Engine ering_Technologies https://pharmavn.org/nobel-hoa-hoc-2020-tim-hieu-ve-crispr-cas-vacac-cot-moc-phat-trien.html Thank You!