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• Zafar Sajjad Shahbaz Alam, “Magnetic Resonance Spectroscopy: Basic principles and application in focal brain. lesions”[r]

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MR‐SPECTROCOPY:

TECHNIQUE AND APPLICATION OFTHE

BRAIN

LÊ THANH PHONG R

August 24, 2019

LÊTHANHPHONG.Rt LÊVĂNPHƯỚC.Prof CAOTHIÊNTƯỢNG.PhD

•Principles and methods

CONTENTS

•Technique

•Metabolites of MRS

•Application

C l i

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PRINCIPLES

•Magnectic Resonance Spectroscopy – MRS: is a new

technique to assess various metabolites in the brain technique to assess various metabolites in the brain

•MR Imaging and Spectroscopy share the same physical

principle MRI looks at the geometrical distribution of water molecules

•MRS looks at the chemical composition of the tissue of interest and displays it in a spectrum

PRINCIPLES

•MRS was approved by the US Food and Drug Administration (FDA) in 1995

(FDA) in 1995

•MRS can be performed with spins or nuclei of Hydro (H), Phospho (P) and Carbon (C)

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MRS nomal human brain In 1.5T MR system TE = 135 ms NAA peak highest

METHODS

There are two basic methods:

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STEAM

•The volume of interest is excited by three 90 degree pulses

•Allows short echo times

•Since the echo is stimulated signal is weak

•Voxel selected

PRESS

•One 90 degree and 180 degree pulses are applied along three orthogonal planes

three orthogonal planes

•Allows longer and shorter echo times

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TECHNIQUES

* There are two techniques:

•Single voxel ( SV )

•Multi voxel ( MV )

SINGLE VOXEL MULTI VOXEL

SINGLE VOXEL

•Voxels must be place beside the sources of magnetic

sensitive images and lipids

•SVS can be used volume 2x2x2 cm (8cm3) for diffuse

diseases

•Volume can decrease for local diseases

•SVS ll t k i t f d t i iti

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MULTI VOXEL

•Known as Chemical Shift Imaging (CSI)

•A large area is covered and divided into multiple voxels

•Acquisition of multiple voxels Many voxels within the same dataset

•Can be slice selected of or dimensionalCan be slice selected of or dimensional

•Single spectra related to single voxels:

•Metabolite images:

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ECHO TIMES

•Data acquisition depends on TR and TE, because brain

metabolites have different T1 and T2 metabolites have different T1 and T2

•TR and TE are important parameters

•Improved SNR is obtained at a longer TR ( #1500ms )

•Commonly used TEs are 20‐30ms, 135‐145ms and 270ms.Commonly used TEs are 20 30ms, 135 145ms and 270ms

* Commonly metabolites have short TE and long TE: * N‐acetyl aspartate (NAA)

* Creatine (Cr) * Choline (Cho) * Lactate (Lac)

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TE = 30ms TE = 135ms TE =270ms

ATTENTIONS!

•Instruct the patient to keep still

•The location of voxel must avoid blood, products of blood,The location of voxel must avoid blood, products of blood, gas, cerebrospinal fluid, fat, areas of necrosis, metal, calcium and bone

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High quality spectrum Low quality spectrum

CORRECTIVE MEASURE

•Used larger bandwidth to improved SNR → longerg p g acquisition times

•Select another voxel location

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MAJOR METABOLITES OF 1H‐MRS

Metabolites Peakpositioninppm Secondpeak

NAA: N‐acetyl aspartate 2.02ppm

Cr: Creatine 3.0ppm 3.94pmm

Cho: choline 3.22ppm

mI: Myo‐Inositol 3.56ppm 4.1ppm

Lac: Lactate 1.3ppm

Lac: Lactate 1.3ppm

Glx: Glutamate Glutamine 2-2.45ppm 3.6-3.8ppm

Lipids 0.9, 1.3, 1.5ppm

CLINICAL USES OF 1H‐MRS

•Brain tumors, Stroke, Close head trauma

•Neonatal hypoxia.Neonatal hypoxia

•Metabolic disorders and white matter diseases

•Epilepsy and Multiple Sclerosis

•Alzheimer’s dementia

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*Cho increase

*NAA and Cr decrease *Latate: doublet

Astrocytoma grade III

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*Cho: normal *NAA decrease

Abscess

Metastasis

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TE 30 TE 135 TE 270

TE = 30ms TE = 135ms TE =270ms

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CONCLUSION

•Select the single voxel or multi voxel?

S l i f TE i

•Selection of TE are important parameters

•Select the voxel location correctly to get a high quality spectrum

•MRS can not replace MRI, but it provides more information to diagnosis.g

•Can used in 1.5T or 3T MR systems

REFERENCES

• Ứng dụng tiến bộkỹthuật MRI lĩnh vực thần kinh sọ PGS.TS Lê văn Phước Trưởng khoa CĐHA BV Chợ

Rẫy

• Nguyễn ThịÁnh Hồng, “Cộng hưởng từphổtrong chẩnđốn u não”, tạp chí y học Thành phốHồChí Minh 2003

• Nguyễn ThịÁnh Hồng, “Bướcđầu khảo sát MRS u não", 2001

• Luận văn nghiên cứu “cộng hưởng từphổtrong khảo sát u não” BS CK2 Cao Thiên Tượng, Khoa CĐHA BV Chợ

Rẫy

• Magnectic resonance spectroscopy of the brain, review of metabolites and clinical applications, D.P Soaresa and

M.Lawb, clinical radiology volume 64, Issue 1, January 2009

• Brandao Lara A.Domigues Romeu C “ MR spectroscopy of the brain” 2004

• Zafar Sajjad Shahbaz Alam, “Magnetic Resonance Spectroscopy: Basic principles and application in focal brain

lesions” Pak J Neurol Sci 2007 lesions Pak J Neurol Sci 2007

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