Mechanism of vortex assisted liquid liquid microextraction of strontium in water sample

THAI NGUYEN UNIVERSITY UNIVERSITY OF AGRICULTURE AND FORESTRY CHU NGUYEN THE STUDY MECHANISM OF VORTEX-ASSISTED LIQUID-LIQUID MICROEXTRACTION OF STRONTIUM IN WATER SAMPLE BACHELOR THESIS Study mode : Full-time Major : Environmental Science and Management Faculty : Advanced Education Program Office Batch : 2014 - 2018 Thai Nguyen 24/9/2018 DOCUMENTATION PAGE WITH ABSTRACT Thai Nguyen University of Agriculture and Forestry Degree Program Bachelor of Environmental Science and Management Student name Chu Nguyen Student ID DTN1453150016 Thesis Title Supervisors Mechanism of vortex-assisted liquid-liquid microextraction of Strontium in water sample - Prof Wu ,Chien-Hou - Prof Nguyen The Hung Supervisor’s Signature Abstract: A vortex-assisted liquid–liquid microextraction method was applied in many years ago, however the first time it was developed for the chromatographic determination of strontium (alkaline-earth) in aqueous samples in 2017 In the extraction , strontium was in aqua phase with the presence of tetraphenylborate as the counter anion, while organic phase (1- octanol was chosen) was complexed with 4′,4″(5″)-di-(tert-butylcyclohexano)-18-crown-6 (7:1 respectively ) Strontium from the organic phase was stripped with nitric acid back to aqueous solution and determined by ion chromatography By changing the concentration of 4′,4″(5″)-di-(tert-butylcyclohexano)-18-crown6 and tetraphenylborate, with standard conditions as vortex for 10s; centrifugation at 6000 rpm for min; stripping by 0.1 M nitric acid and lightproof condition, the result is that with [TPB]=0,003 M and [DtBuCH18C6]= 0,01 M, the extraction of Sr is i optimum with Recovery rate= 79% ,and distribution coefficient logD =1,22 Key words Strontium, tetra phenyl borate, ion chromatography, strontium, vortex-assisted liquid–liquid micro-extraction Number of pages 50 Date of Submission: 24/09/2018 ii ACKNOWLEDGEMENT To have completed this thesis, in addition to the ongoing efforts of myself, I would like to thank for teachers in Advanced Education Program Office as well as teachers in Thai Nguyen University of Agriculture and Forestry, who have dedicated teaching to me the valuable knowledge during study time in university and gave me a chance to my thesis oversea It is with immense gratitude that I acknowledge the support and help of Biomedical Engineering & Environmental Science Department, National Tsing Hua University for accepting me to working in this wonderful place Furthermore, express my sincere deepest gratitude to Prof Wu Chien Hou, from Biomedical Engineering & Environmental Science Department, National Tsing Hua University,who provided physical conditions in laboratory, documents and allowed me to trigger my experiments by myself, and Prof Nguyen The Hung from Thai Nguyen University of Agriculture and Forestry, who guided and created favorable conditions for me during the implementation of this thesis Next, i would like spend special thanks to Ms Pham Thi Hai Van - MSc student who suggested, directly guided to research my thesis, Ms Yang ziruo who teach me tips, principle and working-skills in the laboratory and usage of all devices used in my experiments Besides, they provided the information and data necessary for my implementation process and helped me finish this thesis Finally, I would like to sincerely thank my family, all of my friends who always beside me all the time, giving spiritual help for me complete the tasks assigned during learning and doing this thesis experiment iii In the process of implementing the project, my thesis might have inevitable shortcomings Therefore, I appreciate very much if I may receive the attention and feedback from teachers and friends for this thesis is more completion Sincerely, Chu Nguyen iv TABLE OF CONTENT DOCUMENTATION PAGE WITH ABSTRACT i ACKNOWLEDGEMENT iii TABLE OF CONTENT v LIST OF TABLES vii LIST OF FIGURES viii LIST OF ABBREVIATIONS ix PART I INTRODUCTION 1.1 Research rationale 1.2 Objectives of the research 1.3 Research questions and hypothesis 1.4 Limitations of research PART II LITERATURE REVIEW 2.1 Strontium 2.1.1 The properties of strontium 2.1.2 The interaction of strontium with environment 2.1.3 Effects of Strontium to human‘s health 2.2 Method Review 11 2.2.1 Vortex-assisted liquid–liquid micro-extraction 11 2.2.1 a Vortex-assisted liquid–liquid micro-extraction concept and mechanism 11 2.2.1.b The factors affect to the vortex-assisted liquid-liquid microextraction 13 2.2.1.c Advantages of VALLME and applications 14 2.2.2 Crown ether- DtBuCH18C6 15 2.2.3 Sodium tetraphenylborate 18 2.2.4.Ion Chromatography 20 2.2.4.a Ion Chromatography mechanism 20 2.2.4.b Ion chromatography system 21 PART III METHODS 26 3.1 Material 26 v 3.1.1 Chemical materials 26 3.1.2 Instrumentation 26 3.2 Methods 27 3.2.1 Micro-extraction procedure 27 3.2.2 Analysis 28 PART IV RESULTS 29 4.1 The effect of DtBuCH18C6 and TPB concentration to result of Strontium extraction.29 4.2 Calibration Curve 33 PART V DISCUSSION AND CONCLUSION 34 5.1 DISCUSSION 34 5.2 CONCLUSION 34 REFERENCE 35 vi LIST OF TABLES Table 2.1 The properties of strontium Table 2.2 Properties of 4’,4’’(5’’)-di-tert-butyldicyclohexano 18-crown-6 (DtBuCH18C6) (12)( en.wikipedia.org) 18 Table 2.3 Properties of sodium tetraphenylboron (NaTPB)(13 ) ( en.wikipedia.org) 19 Table 2.4 Function of parts in an IC system 23 vii LIST OF FIGURES Figure.2.1 Applications of VALLME procedure in real samples (C Bosch Ojeda ,F Sánchez Rojas, 2014) 15 Figure 2.2 Ion Chromatography System Configuration 22 Figure 3.1 A vortex-assisted liquid–liquid microextraction process 27 Figure 4.1 The variance of Peak Area of Sr2+ when DtBuCH18C6 concentration is changed 29 Figure 4.2 The variance of Peak Area of Sr2+ when TPB concentration is changed 29 Figure 4.4 Effect of TPB concentration on the distribution coefficient of Sr 31 Figure 4.5 Effect of DtBuCH18C6 concentration on the distribution coefficient of Sr 31 Figure 4.6 Extraction of Sr as a function of DtBuCH18C6 concentration 32 Figure 4.7 Calibration Curve 33 viii LIST OF ABBREVIATIONS Aqueous Phase AP Distribution Coefficient D Aqueous Sample DP 4’,4’’(5’’)-Di-Tert-butyldicyclohexano 18-crown-6 DtBuCH18C6 Ion Chromatography IC Liquid–Liquid Microextraction LLE Strontium Sr Vortex-Assisted Liquid–Liquid Microextraction VALLME ix ... vortex- assisted liquid- liquid microextraction of Strontium in water sample - Prof Wu ,Chien-Hou - Prof Nguyen The Hung Supervisor’s Signature Abstract: A vortex- assisted liquid liquid microextraction. .. applied in many years ago, however the first time it was developed for the chromatographic determination of strontium (alkaline-earth) in aqueous samples in 2017 In the extraction , strontium was in. .. (1037-1050), (2003) 35 Chin-Yi Wang, Da-An Chang, Yuzhou Shen, Yuh-Chang Sun, Chien-Hou Wu 2017, Vortex- assistedliquid liquid microextraction of strontium from water samples using4′,4″(5″)-di-(tert-butylcyclohexano)-18-crown-6