link.springer.com LC–MS/MS-Based Metabolome Analysis of Biochemical Pathways Altered by Food Limitation in Larvae of Ivory Shell, Babylonia areolata AuthorsAuthors and affiliations 17-22 phút Jingqiang Fu Minghui Shen Yawei Shen Wengang Lü Miaoqin Huang Xuan Luo Jinjin Yu Caihuan Ke Weiwei You Jingqiang Fu o o Minghui Shen o o o Yawei Shen o o Wengang Lü o Miaoqin Huang o o Xuan Luo o o o Jinjin Yu o o Caihuan Ke o o o Weiwei You o o o Email authorView author's OrcID profile 1.State Key Laboratory of Marine Environmental ScienceXiamen UniversityXiamenPeople’s Republic of China 2.College of Ocean and Earth SciencesXiamen UniversityXiamenPeople’s Republic of China 3.Tropical Marine Products Fine Breed CenterHainan Academy of Ocean and Fisheries SciencesHainanPeople’s Republic of China 4.College of FisheriesGuangdong Ocean UniversityZhanjiangPeople’s Republic of China 5.Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological ResourcesXiamenPeople’s Republic of China Original Article First Online: 20 April 2018 Abstract Ivory shell, Babylonia areolata, is one of the commercially important mariculture species in China and South East Asia Survival varies in the artificial hatching and larval rearing of B areolata Food deprivation may be involved in rearing mortality, and so, a better understanding of how larvae respond and adjust to starvation is needed In this study, the metabolite profiles of newly hatched larvae with yolk (I), larvae with yolk exhaustion (II), larvae suffering 24 h starvation after yolk exhaustion (III), and larvae fed with exogenous nutrients after yolk exhaustion (IV) were analyzed by LC–MS/MS Principal component and cluster analyses revealed differential abundance of metabolite profiles across groups When compared to metabolite levels of the I group, significantly up-regulated metabolites included polyunsaturated fatty acids, phospholipids, nucleotide, amino acids, and their derivatives were found in the II group, indicating that organisms relied predominantly on glycerophospolipid metabolism and protein-based catabolism for energy production during this stage During starvation after yolk exhaustion, the levels of all energy related metabolites were significantly reduced, but an increase in products of purine and pyrimidine metabolism indicated an insufficient energy supply and an increase in cellular disintegration Larvae fed exogenous nutrients can have significantly improved metabolism compared to starved larvae These findings suggest that metabolomics, using LC–MS/MS, can be used to assess the physiological status and food-affected metabolic changes affecting B areolata larvae Keywords Babylonia areolata LC–MS/MS Metabolomics Newly hatched larvae Food limitation This is a preview of subscription content, log in to check access Notes Acknowledgements The authors thank the Hainan Academy of Ocean and Fisheries Sciences for its assistance in operations over the larval periods This 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Characterization of the human tear metabolome by LC MS/ MS J Proteome Res 10:4876–4882CrossRefPubMedGoogle Scholar 12 Chen Y, Ke CH, Zhang SY, Dai XJ (2017) Feeding rate responses of Babylonia formosae... were analyzed by LC MS/ MS Principal component and cluster analyses revealed differential abundance of metabolite profiles across groups When compared to metabolite levels of the I group, significantly... metabolomics, using LC MS/ MS, can be used to assess the physiological status and food-affected metabolic changes affecting B areolata larvae Keywords Babylonia areolata LC MS/ MS Metabolomics Newly