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Oxford Textbook of Transplant Anaesthesia and Critical Care Oxford Textbooks in Anaesthesia Oxford Textbook of Anaesthesia for the Elderly Patient Edited by Chris Dodds, Chandra M. Kumar, and Bernadette Th Veering Oxford Textbook of Anaesthesia for Oral and Maxillofacial Surgery Edited by Ian Shaw, Chandra M. Kumar, and Chris Dodds Principles and Practice of Regional Anaesthesia, Fourth Edition Edited by Graeme McLeod, Colin McCartney, and Tony Wildsmith Oxford Textbook of Cardiothoracic Anaesthesia Edited by R. Peter Alston, Paul S. Myles, and Marco Ranucci Oxford Textbook of Transplant Anaesthesia and Critical Care Edited by Ernesto A. Pretto, Jr., Gianni Biancofiore, Andre De Wolf, John R. Klinck, Claus Niemann, Andrew Watts, and Peter D. Slinger Free personal online access for 12 months Individual purchasers of this book are also entitled to free personal access to the online edition for 12 months on Oxford Medicine Online () Please refer to the access token card for instructions on token redemption and access Online ancillary materials, where available, are noted at the end of the respective chapters in this book Additionally, Oxford Medicine Online allows you to print, save, cite, email, and share content; download high-resolution figures as Microsoft PowerPoint slides; save often-used books, chapters, or searches; annotate; and quickly jump to other chapters or related material on a mobile-optimized platform We encourage you to take advantage of these features If you are interested in ongoing access after the 12-month gift period, please consider an individual subscription or consult with your librarian Oxford Textbook of Transplant Anaesthesia and Critical Care Editor-in-Chief Ernesto A. Pretto, Jr Editors Gianni Biancofiore Andre De Wolf John R. Klinck Claus Niemann Andrew Watts Contributing Editor Peter D. Slinger 1 Great Clarendon Street, Oxford, OX 2 6DP, United Kingdom Oxford University Press is a department of the University of Oxford It furthers the University’s objective of excellence in research, scholarship, and education by publishing worldwide Oxford is a registered trade mark of Oxford University Press in the UK and in certain other countries © Oxford University Press 2015 The moral rights of the authors‌have been asserted First Edition Published in 2015 Impression: 1 All rights reserved No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, without the prior permission in writing of Oxford University Press, or as expressly permitted by law, by licence or under terms agreed with the appropriate reprographics rights organization Enquiries concerning reproduction outside the scope of the above should be sent to the Rights Department, Oxford University Press, at the address above You must not circulate this work in any other form and you must impose this same condition on any acquirer Published in the United States of America by Oxford University Press 198 Madison Avenue, New York, NY 10016, United States of America British Library Cataloguing in Publication Data Data available Library of Congress Control Number: 2014948994 ISBN 978–0–19–965142–9 Printed in China by Asia Pacific Offset Ltd Oxford University Press makes no representation, express or implied, that the drug dosages in this book are correct Readers must therefore always check the product information and clinical procedures with the most up-to-date published product information and data sheets provided by the manufacturers and the most recent codes of conduct and safety regulations The authors and the publishers not accept responsibility or legal liability for any errors in the text or for the misuse or misapplication of material in this work Except where otherwise stated, drug dosages and recommendations are for the non-pregnant adult who is not breast-feeding Links to third party websites are provided by Oxford in good faith and for information only Oxford disclaims any responsibility for the materials contained in any third party website referenced in this work Foreword Organ transplantation began with kidney transplantation in the early 1950s and expanded to heart, lung, liver, pancreas, multiviscera, and xenograft transplantation Its evolution over the past 50 years or so has been dramatic The experimental stage of organ transplantation (1960–1980) evolved into the developmental stage (1980–1990) and refinement stage (1991–present) with excellent clinical outcomes This evolution has been made possible by better understanding of the scientific basis of transplantation, which includes immunology and pathophysiology and continuously improving surgical technique and perioperative care It is noteworthy that the role of anaesthesiologists and intensivists has been vital in its progression, because organ transplantation is a medical field that requires a multidisciplinary approach by dedicated physicians and scientists of all medical fields I am extremely pleased to witness the publication of the Oxford Textbook of Transplant Anaesthesia and Critical Care edited by Drs Biancofiore, De Wolf, Klinck, Niemann, Pretto, Slinger, and Watts, who are my respected colleagues and experts in the transplantation field The major strength of this textbook can be found in its comprehensiveness and the inclusion of scientific bases It begins with the history of organ transplantation and includes ethics, organ donation, and perioperative management of all transplanted organs The molecular basis of organ transplantation should encourage further exploration in organ preservation, prevention of ischaemic injury, and modulation of rejection phenomena The book presents many ideas and concepts to guide the future direction of transplant anaesthesiology, critical care and research, a vital issue for further development of organ transplantation Organ transplantation, particularly the field of anaesthesiology and critical care medicine, was once believed to be a ‘black hole’—an amorphous unknown object with a huge mass absorbing all resources without a trace We now have a much better understanding of that black hole, and I believe that we are better equipped to explore the unknown universe with the guidance of this textbook As a long-time anaesthesiologist in the transplantation field, I sincerely appreciate all editors and contributors for their life-time dedication to organ transplantation Yoogoo Kang, MD Professor, Anesthesiology Director, Hepatic Transplantation Anesthesiology Thomas Jefferson University Preface So the LORD GOD caused a deep sleep to fall upon the man, and he slept; then He took one of his ribs and closed up the flesh at that place —Genesis 2:21 Organ transplantation is a relatively new medical science that has made it possible for patients with an irreversibly damaged organ, and little hope of long-term survival, to receive a replacement organ from another human-being, living or deceased This ancient idea, once also the purview of science-fiction novels, has become reality, and it is revolutionizing medical care by bringing renewed hope for healing to thousands of patients worldwide, every day The only limitation to a much wider application of this life-saving technology is the scarcity of suitable organs The successful and steady evolution of the field of clinical organ transplantation over the past 100 years or so is directly attributable to the multidisciplinary nature and scientific basis of transplant care In fact, only since 1995 during which time medical technology has made great strides forward have we seen marked improvements in long-term survival, including among recipients of the most complex transplant procedures In particular, advances in perioperative and critical care management of the transplant recipient have accounted to a great extent for these recent improvements in outcomes One of those seminal achievements was the initiation by a group of anaesthesiologists and intensive care physicians at the University of Pittsburgh of a multidisciplinary approach to liver transplantation The first gathering of this group in 1982 was called ‘Anesthesia and Perioperative Care in Liver Transplantation’, which later became the International Liver Transplantation Society (ILTS) In a letter dated August 1993 by Dr Thomas Starzl, a renowned American liver transplant pioneer, to Dr Peter Safar, a critical care medicine pioneer and founding Chairman of the Department of Anesthesiology and Critical Care Medicine at the University of Pittsburgh, who was then director of what is now known as the Safar Center for Resuscitation Research, Starzl wrote:  . . I have always taken pains to point out how what we would be utterly impossible without the marvelous collaboration of our unselfish colleagues who work in the operating room and slave over these terribly sick patients afterwards In a much more professionally meaningful but less public way, we have promoted the interests of the anesthesia and ICU physicians by passing the leadership baton onto Kang et  al., for the organization of the International Liver Transplantation Society that will be meeting for the second time this year in Canada Dr.  Kang’s efforts and those of his intensive care colleagues were responsible for the birth of this very important organization . . .  In the same vein, the American Society of Anesthesiologists (ASA) described the nature of anaesthesia practice for organ transplantation, and those anaesthesiologists and intensivists engaged in its delivery, in the following manner: The complexity of transplant surgeries requires the expertise and specialty of a transplant anesthesiologist who is an integral part of the transplant team Transplant anesthesiologists have an extensive background in critical care medicine, cardiac anesthesiology and/ or pediatric anesthesiology This type of anesthesiologist also provides consultation in both the preoperative and intraoperative stages of care This statement was a first step in the direction of defining the knowledge base, required training and experience, and scope of practice of the ‘transplant anaesthesiologist’ Likewise, a recent ‘landmark’ decision by the Organ Procurement and Transplantation Network (OPTN)/United Network for Organ Sharing (UNOS) (the principal government-mandated agency overseeing organ procurement and sharing in the US), in partnership with the ASA Committee on Transplantation, has promulgated guidelines for the qualifications and clinical responsibilities of the director of liver transplant anaesthesia programmes, as follows: The director of liver transplant anesthesia should have one of the following: Fellowship training in Critical Care Medicine, Cardiac Anesthesiology, or a Liver Transplant Fellowship, that includes the peri-operative care of at least 10 liver transplant recipients Experience in the peri-operative care of at least 20 liver transplant recipients in the operating room, within the last 5 years Experience acquired during postgraduate residency training does not count for this purpose viii  preface The director of liver transplant anesthesia has clinical responsibilities that include but are not limited to the following: ◆ Pre-operative assessment of transplant candidates ◆ Participation in candidate selection ◆ Intra-operative ◆ Post-operative management visits ◆ Participation on the Selection Committee ◆ Consultation pre-operatively with subspecialists as needed ◆ Participation in morbidity and mortality (M&M) conferences (UNOS Bylaws) These guidelines provide official recognition that expertly trained anaesthesiologists are critical to optimizing transplant care The UNOS bylaws endorse transplant anaesthesiologists’ active participation in medical decision-making in all facets of transplant care We believe these guidelines should not be limited to the care of liver transplant recipients alone but that the benefits of multidisciplinary care and specialized training in transplant anaesthesia will inevitably lead to improved care for all types of organ transplant recipients, as well as organ donors Other than pain management and critical care medicine, perhaps there is no other subspecialty of anaesthesiology where our involvement has been as important to improved outcomes and as valued by our professional counterparts Therefore the impetus for a book on ‘Transplant Anaesthesia and Critical Care’ derives from the same spirit of collaboration and collegiality that seeks to affirm the current trend towards greater specialization in transplant anaesthesia and critical care Specifically, this book attempts to define the normative body of scientific and clinical knowledge, skill, and training that is essential to the expertise of the anaesthesiologist involved in the care of the organ donor as well as the organ transplant recipient On behalf of the editors, the expectation is that the appearance of this book is timely, relevant, and true to the present state-ofthe-art and that it will bolster the continued development of the burgeoning field of transplant anaesthesia and critical care As you will see on initial perusal of the table of contents we have taken a holistic approach to the scope of practice of transplant anaesthesia In so doing we cover in depth many aspects of transplantation of the major organ systems Moreover, we have assembled a cadre of multidisciplinary transplant experts from several continents that present updated evidence-based information on various topics related to transplantation, including data on organ transplant practices in leading countries, as well as some of the ethical challenges facing the field of organ transplantation today We hope this book will be well received by the medical and scientific communities and that it will serve as the authoritative reference work in the field, thereby forming the basis for the design of curricula for teaching and training of medical students, anaesthesia residents, transplant anaesthesia fellows, nurse anaesthetists, and attending anaesthesiologists in transplant care Finally, we believe that the future of transplant anaesthesia relies heavily on discovery As such we hope this book will provide the reader with key insights into cellular mechanisms of ischaemia–reperfusion injury that will inspire transplant anaesthesiologists to engage in basic and clinical research, and in so doing contribute to the collective advancement of the science of transplantation Ernesto A. Pretto, Jr., MD, MPH Miami Transplant Institute Contents Abbreviations  xiii Editors  xix Contributors  xxi SEC TION 1 Introduction History of organ transplantation  John R. Klinck and Ernesto A. Pretto, Jr 2 The development of organ donation systems and regulatory bodies in the United States  13 Nikole Neidlinger, Sean Van Slyck, and Daniel M. Bruggebrew Organ donor allocation and transplant logistics: the European perspective  23 Gabriela A. Berlakovich and Thomas Soliman SEC TION 2 Introduction to transplant ethics Acquiring organs ethically: problems and prospects  33 Jamie Lindemann Nelson Organ allocation: a guide for the perplexed  43 Benjamin E. Hippen Ethical issues in transplant tourism and organ commercialism  53 Robin N. Fiore SEC TION 3 The organ donor Neurological determination of death and organ donation  61 Shariq S. Raza, Ali Salim, and Darren J. Malinoski Critical care of the organ donor  69 Matthew B. Bloom, Ali Salim, and Darren J. Malinoski Research in organ donors: future directions  77 Claus Niemann and Andrea Olmos SECTION 4 The scientific basis of organ transplantation 10 Organ resuscitation  87 Ernesto A. Pretto, Jr., Kyota Fukazawa, and Antonello Pileggi 11 Transplant immunology  99 Phillip Ruiz, Junichiro Sageshima, George Burke, Linda Chen, and Gaetano Ciancio 12 Xenotransplantation  111 Kazuhiko Yamada, Joseph Scalea, and Masayuki Tasaki SECTION 5 Kidney and kidney–pancreas 13 Indications, selection, and evaluation of the kidney transplant candidate  125 M Francesca Egidi and Giuseppe Segoloni 14 Kidney transplantation: perioperative cardiovascular risk and anaesthetic management  131 Livia Pompei, Maria Gabriella Costa, Mauricio Sainz-Barriga, George Burke, and Giorgio Della Rocca 15 Perioperative management of the kidney–pancreas and pancreas transplant recipient  141 Ugo Boggi, George Burke, and Kumar Belani 454 index heart–lung transplantation (Cont.) indications 315 intensive care  349 intubation 317 liver dysfunction  316 non-surgical bleeding  318–19 one-lung ventilation  317 postoperative management  319 pregnancy and  434–5 preoperative assessment  315–16 previous surgery  316 primary graft dysfunction  318 renal dysfunction  316 reperfusion 318 respiratory assessment  316 right ventricular dysfunction/ failure 318 surgical team  315 TOE 317 heart transplantation acute rejection  312, 343–4 anaesthesia induction  309–10 anaesthesia maintenance  310 anaesthetic management  307–11 antifibrinolytics 310–11 atrial fibrillation  311 awareness risk  309 cardiac allograft vasculopathy  312, 344, 360 cardiopulmonary exercise testing  307 children, see paediatric heart transplantation contraindications 309 denervated heart  341 dysrrhythmias 311, 340 endomyocardial biopsy  344 heart rate and rhythm  340 history of 6 immunosuppression  312, 342, 343 indications 307 infection risk  311, 342–3 inhaled nitric oxide  311 intensive care  339–4 intraoperative monitoring  309 logistics 27 long-term complications  312 malignancy risk  344 non-transplant surgery  424–5 organ allocation  27 organ donor management  307 pacing 311, 340 paediatrics, see paediatric heart transplantation physiology and pharmacology of transplanted heart  340–2 postoperative bleeding  311 postoperative management 311–12, 339–40 pregnancy and  434–5 preload and afterload  340 premedication 309 preoperative assessment  307–9 prostacyclin 311 prostaglandin E1 311 pulmonary hypertension  307, 309 right heart function  341–2 separation from cardiopulmonary bypass 311 survival 307 TOE 309 transition to ICU  339 transpulmonary gradient  308–9 ventricular assist device  342, 357 waiting list management  307 xenotransplantation 111 heparin 398 heparinase 397 hepatic encephalopathy  191, 213–14, 215, 244–6, 261, 262, 268 hepatitis B 183 hepatitis C  183, 184, 206 hepatocellular carcinoma cell salvage  409–10 epidemiology 183 indications and selection criteria for transplantation 201, 202 referral guidance for transplantation 207 hepatopulmonary syndrome  192–3, 227, 260, 270 hepatorenal syndrome  189, 260 high urgency requests  26 histamine 246 histidine–tryptophan–ketoglutarate solution 5 histocompatibility clinical laboratories 101 history of transplantation  3–10, 111, 353 HIV 15, 206 HLA antibodies  125, 300, 332, 357, 386–7 HLA class I/II molecules  99 HLA typing  101 HOPE Act 15 hormonal deficiencies, neurological death 71 hormone replacement therapy  73 hospital development  17–18 host versus graft response  100–1 HTK solution  hydrocortisone 102 hydrothorax 189 hydroxyethyl starch  136, 254 hyperacute rejection  112 hyperkalaemia  194, 223, 292–3, 415 hypermetabolism 194 hypertrophic cardiomyopathy  353 hypokalaemia 292–3 hyponatraemia 223, 262 hypoplastic left heart syndrome  354 hypothermia 72, 388 hypothyroid state  71–2 hypoxanthine 90, 92 hypoxia-inducible factor (HIF-1)  88 I idiopathic pulmonary fibrosis  321–2, 324 illicit substance use  206 immunizations, pretransplant  240, 261 immunology 99–105 pharmacological modulation of immune response  101–5 tissue rejection  99–101 tissue typing and organ matching  101 xenotransplantation 112–14 immunosuppression agents 101–5 geriatric transplantation  377, 380 heart transplantation  312, 342, 343 history of 4–5 induction therapy  105 intestinal and multivisceral transplantation  282, 300–1, 302 islet cell transplantation  172, 174, 176 kidney–pancreas transplantation 148, 156 kidney transplantation  156 lung transplantation  348 maintenance therapy  105 non-transplant surgery  420–1 paediatric liver transplantation  269 pancreas transplantation  148, 156 pregnancy after transplantation  430–1 protocols 105 xenotransplantation 112, 116–17 index of covariance  50 infection cell salvage  410 fibrosis 101 heart transplantation  311, 342–3 intestinal and multivisceral transplantation 299 kidney–pancreas transplantation  153–6 kidney transplantation  153–6, 375 liver disease  193–4, 217–18 liver transplantation  239–40 lung transplantation  347–8 non-transplant surgery  421–2 paediatric heart transplantation  360 paediatric liver transplantation  271–2 pancreas transplantation  153–6 pretransplant screen  145 TEG®effects 399 transfusion-related 391 informed consent  25, 35–6, 79, 81–2 inhaled nitric oxide  311, 333 inosine monophosphate dehydrogenase inhibitors 104 index insulin  72, 142, 156, 167 Intensive Care Units 5; see also critical care International Society for Heart and Lung Transplantation (ISHLT)  21 intestinal transplantation abdominal wall closure  281–2 anaesthesia maintenance  281 arterial line  280 cardiovascular risk  279 central venous lines  281 contraindications 278, 280 critical care  297–302 definition 277 dissection phase  281 early extubation  297 endoscopic surveillance  299, 301 GI tract reconstruction  281 graft perfusion and viability  297 graft versus host disease  299–300 haematological monitoring  298 history of 7 immunosuppression  282, 300–1, 302 indications 278, 279 infection control  299 intraoperative management  281 intraoperative monitoring  279–81 living donors  286 nutrition 298 paediatrics, see paediatric intestinal and multivisceral transplantation postoperative management 282–3, 297–8 post-transplant lymphoproliferative disorder 299, 302 pregnancy and  435–6 pretransplant assessment  278–9 pulmonary artery catheter  280 quality of life  301–2 rejection surveillance  299 renal function  297 reperfusion 281 surgical technique  281–2 survival rates  277 tube feeding  283 vascular access  279 vascular anastomosis  281 intra-abdominal pressure  271 intracranial hypertension  213–15, 268 intracranial pressure monitors  404 intraoperative cell salvage, see cell salvage intravenous immune globulins  104 Iran Iranian Network for Organ Procurement 20 transplant tourism  56 ischaemia 87–91 ischaemia–reperfusion injury (PGD)  242–3, 256, 318, 333, 346–7 ischaemic heart disease  225–6 ischaemic preconditioning  95 islet cell transplantation  171–7 adverse events  175–6 allogeneic transplant  171 autologous transplant  171 autotransplant 172 benefits 175 cell isolation and processing  172 clinical monitoring  174 concomitant therapy  174 costs issues  176 exclusion criteria  173 extrahepatic sites  172, 176–7 graft function monitoring  174–5 hypoglycaemia prevention  175 immunosuppression  172, 174, 176 indications 171–2, 173 insulin independence  175 outcomes 175 progressive loss of insulin independence 176 stem cells  176 transplantation techniques  172 xenotransplantation 117, 176 islets of Langerhans  142–3, 166 Israel, transplant tourism  55 J Japan Organ Transplant Network  20 jugular venous oximetry  404–5 K ketorolac 135 kidney disease, global context  53 kidney–pancreas transplantation acute rejection  149 anaesthesia induction  145–6 anaesthetic management  144–7 antithrombotic prophylaxis  148 arterial in-flow  143 arterial lines  145 bladder drainage  143, 148, 149 burnt-out pancreas  149 cardiac complications  159–61 central venous catheters  145 chronic rejection  149 CMV prophylaxis  148 coronary artery disease screening  159 critical care  153–61 diabetes recurrence  149 diabetic patients  167–9 donor duodenum culture  148 enteric drainage  143 fluid management  146, 158 glucose control  156 haemorrhage 161 history of 7 hypercoagulability 148 immunosuppression 148, 156 infection complications  153–6 infection screen  145 intra-abdominal infections  155 intraoperative planning and care  145 kidney-first 144 laparoscopic and robotic approach  143 metabolic care  146 obese patients  145 operative management  147–8 outcomes 148–9 pancreas-first 143–4 postoperative analgesia  147 postoperative care  147, 148–9 postoperative scans  148 pretransplant evaluation  144–5 regional analgesia  146 reoperation 148 reperfusion 146–7, 148 respiratory failure  156–9 surgical technique  143–4 TEG® 148 thrombotic complications  149, 161 urinary tract infections  155 vascular complications  161 venous drainage  143 kidney transplantation ABO-incompatible 125 aetiology of ESRD  125–6 anaesthetic choice  134–5 anaesthetic management  134–6 candidate selection  128 cardiac surveillance on waiting list 132–3 cardiovascular complications 159–61, 374–5 cardiovascular risk  127, 131–3 coronary artery disease  127, 131, 132, 159 coronary revascularization  127, 132 critical care  153–61 delayed graft function  82–3 donor exchange programmes  125 donor-specific antibodies  125 double-J-ureteral stent  137–8 early referral  125 elderly  26, 45, 374–5 fluid management  135–6, 158 funding 15–16 glucose control  156 haemodynamic management 134, 135–6 haemorrhage 161 history of  3, 6 immunosuppression 156 indications 125–6 infection complications  153–6, 375 intra-abdominal infections  155 living donors  125, 375 455 456 index kidney transplantation (Cont.) logistics 25–6 muscle relaxants  134 non-transplant surgery  423–4 NSAID use  135 organ allocation  26, 44–5, 49–50 outcome 374–5 paediatrics 138 postoperative analgesia  125 postoperative ICU admission  138 pre-emptive 125 pregnancy and  433–4 premedication 134 pretransplant evaluation  126–8 pulmonary evaluation  133–4 reperfusion 137 respiratory failure  156–9 smoking 127, 133 surgical technique  136–8 survival 374–5 thrombotic complications  161 urinary tract anastomosis  137–8 urinary tract infections  155 U-stitch 138 vascular anastomosis  137 vascular complications  161 xenotransplantation 111, 117 killing/letting die distinction  36 KIR 99 Krebs cycle  88 L lactic acidosis  415 Latin America  439–40, 441 legislation, see regulation leucocyte depletion filters  409 leucodepletion 387 levosimendan 310, 331 liberation from ventilation  158 life years after transplant  48 LiMax test  243 live liver donors  9, 26, 195, 230, 251–6 anaesthetic agents  252 blood transfusion  254 central venous pressure monitoring 252–3 donor assessment  251–2 donor morbidity and mortality  255–6 fluid management  253–4 information provision  251 intraoperative management  252–5 intraoperative monitoring  252–3 ischaemia–reperfusion injury  256 laparoscopic techniques  256 left lobe grafts  255 liver regeneration  255 postoperative management  254–5 postoperative pain  255 preoperative assessment  252 prophylactic therapy  252 liver anatomy and physiology  184 elderly patients  372 neurological death  71 regeneration 255 liver disease acute versus chronic liver failure in children 265 clinical manifestations in children 259–61 critical care  211–20 elderly 372–3 end-stage  184, 194–5, 211, 213, 215, 216, 217–18, 219, 224–5, 259, 260–1 epidemiology 183–4 trends 9 viscoelastic haemostatic assays  399 see also acute liver failure;chronic liver disease; hepatocellular carcinoma liver–intestine–pancreas transplantation 288 liver–intestine transplantation  278 liver–kidney transplantation  208 liver support systems  218–19, 262, 268 liver transplantation acute liver failure  201, 207, 212–13 age 204–5 alcohol misuse  205–6 anaesthetic management  8, 230–5 anaesthetic technique  230 anhepatic phase  228 antifibrinolytic therapy  8–9 blood products  232, 389 cardiovascular function  205, 224, 225–7, 231–2, 374 cardiovascular monitoring  231 cava-cavaplasty 228 caval replacement versus preservation 7–8, 288–9 cell salvage  232, 407, 409–10 cerebral monitoring  403–4 children, see paediatric liver transplantation chronic liver disease  195, 201–2, 207 classical (caval replacement) technique 7–8, 228 CNS function  225 coagulopathy management  223, 225, 231, 233–4 contraindications 203–4, 228 critical care  239–47 diabetes mellitus  205 dissection phase  228 donor risk index  379 early extubation  9, 235, 239 EEG 403–4 elderly 372–4 electrolytes 224, 234–5 enteral feeding  244 extrahepatic malignancy  206 fast-tracking 9 fluid management  232–3, 415 glucose control  235 graft function assessment  240–2 haemodynamic management 225, 400–5 hepatocellular carcinoma  201, 202, 207 hepatopulmonary syndrome  227 high urgency requests  26 history of 7–9 HIV 206 HIV–HCV co-infection  206 hyperkalaemia 223, 415 hyponatraemia 223 indications 195, 201–2 infection prophylaxis  239–40 inferior vena cava clamping  228, 231 initial poor function  242 intracardiac thrombosis  233–4 intracranial pressure monitors  404 intraoperative monitoring8,  230–1 ischaemia–reperfusion injury  242–3 jugular venous oximetry  405 lactic acidosis  415 LiMax test  243 living donors, see live liver donors logistics 26 MELD score  mental status  241 metabolic changes  224, 234–5 near-infrared spectroscopy  403 non-transplant surgery  424 nutritional management  205, 227, 244 obesity 205, 227 oesophageal Doppler  402–3 organ allocation  9, 26, 206–7 outcome  195, 207–8, 373–4 paediatrics, see paediatric liver transplantation paracetamol hepatotoxicity  202, 207 parenteral feeding  244 peripheral arterial waveform analysis 402 piggyback (caval preservation) technique 7–8, 228–9 portal vein unclamping  232 portopulmonary hypertension 227, 401 postoperative care 9 pregnancy and  432–3 preoperative evaluation  223–8 preoperative preparation  230 pretransplant evaluation  202–3, 204 pretransplant immunization  240 primary graft dysfunction  242–3 prioritization of recipients  206–7 prognosis 195 pulmonary artery catheter  8, 401–2 pulmonary hypertension  227, 232 rapid infusion systems  9, 410 index referral guidance  207 removal of critically ill patients from waiting list  219–20 renal function  206, 223, 224, 225, 227, 232 reperfusion 229, 232 respiratory function  224, 227, 235 retransplantation 208 sepsis 228 seventh day syndrome  243 smoking 206 split grafts  195 substance misuse  206 super-urgent  201, 202, 213 surgical techniques and procedures 228–30 survival 373–4 TEG®  8, 231, 395 timing of referral  201 TOE 231, 402 transcranial Doppler  403 UKELD score  201–2 urine output  241 vascular access  230 venovenous bypass  8, 229–30 viscoelastic haemostatic assays 395, 398 waiting list management  xenotransplantation 111, 117 living donors  66 donor selection  23–4 ethics 35–6 growth in programmes  9–10 intestinal transplantation  286 kidney transplantation  125, 375 see also live liver donors logistics 25–7 low potassium dextran  325 lung allocation score (LAS)  27 lung-protective strategies  70, 78 lung transplantation acute rejection  347 airway complications  349 anaesthesia induction  328–9 anaesthesia maintenance  330–1 anaesthetic management  327–33 antifibrinolytics 332 bridge to 327 bronchial stenosis  349 bronchiolitis obliterans  425 cardiopulmonary bypass  331–2 children 362–3 complications 346–49 contraindications 326 cystic fibrosis  322–3, 328, 329, 348, 362 donor organ management  324–6 donor selection  324 double lumen tubes  329 dysrrhythmias 348 early mobilization  346 extracorporeal membrane oxygenation 326–7, 332 ex-vivo lung perfusion  325–6 fluid management  332, 345 gastrointestinal complications  349 haemodynamics 345 history of 6 immunosuppression 348 indications 321, 323 infection risk  347–8 intensive care  344–49 interoperative setup and monitoring 326–7 ischaemic time  325 logistics 27 lung allocation score (LAS)  27 lung preservation  324–5 neurological complications  347 non-transplant surgery  425 obstructive lung disease  321, 328, 329 organ allocation  27 paediatrics 362 patient positioning  330 plasmapheresis 332 pneumonia development  347–8 postoperative analgesia  333, 345–6 postoperative management  334, 344–5 preoperative assessment and management 327–8 primary graft dysfunction  333, 346–7 pulmonary vascular disease 323–4, 328–9 recipient selection  326 renal complications  347 reperfusion 332–3 restrictive lung disease  321–2, 328, 329 right ventricular dysfunction  331 suppurative lung disease  322–3, 328, 329, 348 survival 321 thoracic epidural anaesthesia  333, 346 TOE 329–30 ventilator management and weaning 345 M magnetic resonance angiogram  62 major histocompatibility complex (MHC) 99 major incompatibility  385 malignancy cell salvage  409–10 heart transplantation  344 non-transplant surgery  422 paediatric heart transplantation  360 pre-liver transplantation  206 malnutrition  194, 203, 205 mandated data submission  447–8 mannitol 136 marketing organs  38–9, 53–7 MARS®  218–19, 262, 268 massive transfusion  72, 319, 388–9 mechanical circulatory support  357–8 mechanical ventilation  157–8, 216–17 medical competency  441–2 melatonin 246 MELD score  9, 185–6, 187, 289 memantine 246 mentorship 442 metabolic systems elderly 362 kidney–pancreas transplantation  146 liver transplantation  224, 234–5 neurological death  71–2 pancreas transplantation  146 methylprednisolone 78, 102 MICA/MICB 99 microchimerism 391–2 microvascular failure  91 midazolam 270 milrinone  310, 331, 361 minor histocompatibility antigens (mHags) 99 minor incompatibility  385 mitochondrial permeability transition (pore) 93–4 mixed chimerism  112–13 mixed venous oxygen saturation 402 monoclonal antibodies  102–3, 104, 421 moral issues  34–5, 36–8 mTOR inhibitors  104–5, 421 multivisceral transplantation abdominal wall closure  281–2 anaesthesia maintenance  281 arterial line  280 cardiovascular risk  279 central venous lines  281 complications 376 contraindications 278, 376 critical care  297–302 definition 277 dissection phase  281 early extubation  297 elderly 375–6 endoscopic surveillance  299, 301 fluid management  376 GI tract reconstruction  281 graft perfusion and viability  297 graft versus host disease  299–300 haematological monitoring  298 history of 7 immunosuppression  282, 300–1, 302 indications 278 infection control  299 intraoperative management  281 intraoperative monitoring  279–81 non-transplant surgery  424 nutrition 298 outcome 375–6 457 458 index multivisceral transplantation (Cont.) paediatrics, see paediatric intestinal and multivisceral postoperative management 282–3, 297–8 post-transplant lymphoproliferative disorder 299, 302 pretransplant assessment  278–9 pulmonary artery catheter  280 quality of life  301–2 rejection surveillance  299 renal function  297 reperfusion 281 surgical technique  281–2 tube feeding  283 vascular access  279 vascular anastomosis  281 mycophenolate mofetil  104, 420–1, 430 mycophenolic acid  104 myocardial ischaemia  160–1 myocardial stress scintigraphy  159 N National Organ Transplant Act (1984)  14 naturally occurring antibodies  385 near-infrared spectroscopy  403 necrosis 92 neurological death children 63 determination 61–3 donor management goals  72 organ function  64–6 pathophysiology 69–72 neurological function elderly 372 geriatric transplantation  380 hepatic encephalopathy  191, 213–14, 215, 244–6, 261, 262, 268 lung transplantation  347 non-transplant surgery  422 neurosteroids 245 neurotransmission 246 new transplant programmes  439–43 NF-κB 92 NHS Blood and Transplant  25 non-alcoholic fatty liver disease  183, 184 non-heart-beating donors  23 non-invasive ventilation  157, 158–9 non-transplant surgery  419–26 anaesthetic management  422–3 electrolytes 423 glycaemic control  423 haemodynamic control  423 heart transplant recipients  424–5 immunosuppression 420–1 infections 421–2 intraoperative monitoring  423 kidney transplant recipients  423–4 liver transplant recipients  424 lung transplant recipients  425 multivisceral transplant recipients  424 neurological complications  422 perioperative management  419–20 postoperative analgesia  425–6 postoperative management  425–6 post-transplant malignancies  422 preoperative evaluation  422 rejection 421 Noonan’s syndrome  353 no-reflow phenomenon  91 norepinephrine 245 normal saline  254 North American Transplant Coordinators Organization (NATCO)  21 NSAIDs 135 nuclear imaging  63 nutrition  194, 203, 205, 227, 244, 260–1, 272, 298 O obesity  145, 205, 227 obstructive lung disease  227, 321, 328, 329 oesophageal Doppler  402–3 oesophageal varices  186–8 OKT3 421 opioids, liver resection  255 opt-in systems  20 OPTN/UNOS committees  14 opt-out systems  20–1, 36–8 organ allocation  43–51 authority and  46–7 efficiency 47–8 equity 50–1 geographic boundaries in US  15 kidney transplantation  26, 44–5, 49–50 liver transplantation  9, 26, 206–7 logistics 25–7 OPO responsibilities  19 outcomes and 45 pancreas transplantation  26–7 principles 46–51 property rights  39, 46 shortage of organs  43–6 thoracic organs  27 trends 9 utility 48–50 waiting-time based  50–1 organ donation altruism 54 authorization process  18–19, 20–1, 39–40 clinical donor management  19 critical are  64–6, 69–74 development of systems in US  13–21 donor management goals  72–3, 78 ethics 33–41 exploitation 56 family support  18–19, 39–40 HIV-positive 15 interhospital sharing  13 opt-in systems  20 opt-out systems  20–1, 36–8 recovery of organs  19, 73–4 regional sharing  13 regulation  10, 13–21, 24–5, 45–6 research in  77–83 signing up on registries  17 trends 9 vendors 38–9, 53–7 organ matching  101 organ preservation  5, 90–1, 96, 324–5 Organ Procurement and Transplantation Network (OPTN)  14 organ procurement organizations (OPOs) performance measurement  16–17 responsibilities 17–19 structure 15–16 organ recovery critical care considerations  73–4 OPO responsibilities  19 organ resuscitation  94–6 organ sharing systems  19–20 organs transplanted per donor  17 organ transplant continuum  87–93 outcomes kidney–pancreas transplantation  148–9 kidney transplantation  374–5 liver transplantation  195, 207–8, 373–4 multivisceral transplantation  375–6 new transplant programmes  442–3 organ allocation and  45 paediatric heart transplantation 359–60 paediatric lung transplantation  363 pancreas transplantation  148–9, 377 oxidative stress  246 oxindole 245 P pacing 311, 340 paediatric heart transplantation  353–62 ABO-incompatible 358–9 anaesthesia induction  361 anthracycline-induced cardiomyopathy 353 anti-HLA antibodies  357 bridge to  357–8 cardiac allograft vasculopathy  360 cardiomyopathies 353–5 congenital heart disease  354 contraindications 356–9 dilated cardiomyopathy  353–4 donation after cardiac death  359 failed Fontan  355–6 history of 353 hypertension 360 hypertrophic cardiomyopathy  353 index hypoplastic left heart syndrome  354 indications 353–6 infection risk  360 intraoperative management  361 malignancy 360 outcomes 359–60 postoperative management  361–2 post-transplantation surveillance  360 preoperative assessment  360–1 pretransplant assessment  355 pulmonary hypertension  361–2 pulmonary vascular resistance  357 rejection 360 renal dysfunction  360 restrictive cardiomyopathy  354–5 retransplantation 355 right ventricular failure  361 survival 359–60 paediatric intestinal and multivisceral transplantation 285–93 abdominal wall closure  288 anaesthesia induction and maintenance 291 anaesthetic management  288–93 arterial line  291 blood products  290 central venous access  290–1 coagulopathy 293 contraindications 286–7 donor assessment and selection  285–6 GI tract reconstruction  288 glycaemic control  293 hyperglycaemia 293 hyper-/hypokalaemia 292–3 hypotension 292 indications 286 intraoperative indices  292 living donors  286 operating room preparation and equipment 290–1 preoperative assessment  288–90 quality of life  301–2 surgical technique  287–8 vascular anastomosis  288 paediatric kidney transplantations  138 paediatric liver transplantation abdominal compartment syndrome 271 acute liver failure  264–5 anaesthesia induction  262 anaesthetic management  262–5 anhepatic phase  263–4 anti-acid medication  269 antibiotics 269 antiplatelets 270 blood products  271 cardiovascular function  259–60, 270 cell salvage  263 central venous access  262–3 clinical manifestations of liver disease 259–61, 267–8 critical care  267–73 dissection phase  263 enteral feeding  272 failure to wean  272–3 fluid management  263, 270–1 gastrointestinal bleeding  272 graft dysfunction/thrombosis  272 hyponatraemia 262 immunosuppression 269 indications 259, 260 infection control  271–2 intraoperative management  262–5 intraoperative monitoring  262, 263 investigation of liver disease  261 nutrition 260–1, 272 PELD score  261 postoperative analgesia  270 postoperative care  270–2 postoperative sedation  270 preoperative assessment  262 principles of intensive care  269 rejection 272 renal function  260 reperfusion 264 respiratory function  260, 270 retransplantation 265 seizures 271 surgical technique  263–4 temperature control  263 vaccinations 261 ventilation 270, 272–3 waiting list management  261 paediatric lung transplantation  362–3 pain relief, see postoperative analgesia palliative care  268–9 pancreas, anatomy and physiology  141 pancreas transplantation acute rejection  149 anaesthesia induction  145–6 anaesthetic management  144–7 antithrombotic prophylaxis  148 arterial in-flow  143 arterial line  145 bladder drainage  143, 148, 149 blood supply to graft  141–2 burnt-out pancreas  149 cardiac complications  159–61 central venous catheter  145 chronic rejection  149 CMV prophylaxis  148 coronary artery disease screening  159 critical care  153–61 diabetes recurrence  149 diabetic patients  167–9 donor duodenum culture  148 elderly 376–7 enteric drainage  143 fluid management  146, 158 glucose control  146, 156 haemorrhage 161 history of 7 immunosuppression 148, 156 infection complications  153–6 infection screen  145 intra-abdominal infections  155 intraoperative planning and care  145 logistics 26–7 metabolic care  146 obese patients  145 operative management  147–8 organ allocation  26–7 outcome 148–9, 377 physiology of graft  142–3 postoperative analgesia  147 postoperative care  147, 148–9 postoperative scans  148 P-PASS score  27 pregnancy and  436 pretransplant evaluation  144–5 regional analgesia  146 renal function evaluation  145 reoperation 148 reperfusion 146–7, 148 respiratory failure  156–9 surgical technique  143–4 thrombotic complications  149, 161 urinary tract infections  155 vascular complications  161 venous drainage  143 xenotransplantation 117 paracetamol hepatotoxicity  202, 207 parenteral feeding  244 passenger lymphocyte syndrome  385 patent foramen ovale  329 patient blood management  389–91 patient-centred outcomes  442–3 patient satisfaction  449 PCP pneumonia  240 PELD score  186, 261, 289 Perfadex® 325 perfusion preservation  5, 91, 96 peripheral arterial waveform analysis  402 peroxynitrite 92 pharmacological preconditioning  95–6 pharmacology elderly 372 heart transplantation  341 liver disease  194–5 phosphofructokinase 88 physiological stability  153 PiCCO system  402 pig organs, see xenotransplantation Plasmalyte™ 254 Plasmapheresis 332 plastic bronchitis  354 platelet function  397–8 459 460 index platelet transfusion  386 Pneumocystis jirovecii 240 pneumonia  193, 240, 347–8 Pompe’s disease  353 portal hypertension  186–8 portopulmonary hypertension  192, 227, 401 postconditioning 96 post-ischaemia–anoxia 91–3 postoperative analgesia kidney–pancreas transplantation  147 kidney transplantation  125 live liver donors  255 lung transplantation  334, 345–6 non-transplant surgery  425–6 paediatric liver transplantation  270 pancreas transplantation  147 postoperative delirium  380 postoperative management/monitoring geriatric transplantation  380 heart–lung transplantation  319 heart transplantation  311–12, 339–40 intestinal and multivisceral transplantation 282–3, 297–8 kidney–pancreas transplantation 147, 148–9 live liver donors  254–5 liver transplantation  lung transplantation  334, 344–5 non-transplant surgery  425–6 paediatric heart transplantation  361–2 paediatric liver transplantation  270–2 pancreas transplantation  147, 148–9 xenotransplantation 116 post-transplant lymphoproliferative disorder  299, 302, 360 P-PASS score  27 Preconditioning 95–6 prediction models  49–50 pregnancy after transplantation  429–36 graft function evaluation  429–30 heart–lung transplantation  434–5 heart transplantation  434–5 immunosuppression 430–1 intestinal transplantation  435–6 kidney transplantation  433–4 liver transplantation  432–3 pancreas transplantation  436 timing of pregnancy  429 pre-ischaemia 87 preload 340 preoperative assessment geriatric transplantation  377–9 heart–lung transplantation  315–16 heart transplantation  307–9 live liver donors  252 liver transplantation  223–8 lung transplantation  327 non-transplant surgery  422 paediatric heart transplantation  360–1 paediatric intestinal and multivisceral transplantation 288–90 paediatric liver transplantation  262 pre-preservation 87–90 preservation of organs  5, 90–1, 96, 324–5 pressure–time product  157 presumed consent  20–1, 24 pretransplant evaluation intestinal and multivisceral transplantation 278–9 kidney–pancreas transplantation  144–5 kidney transplantation  126–8 liver transplantation  202–3, 204 paediatric heart transplantation  355 pancreas transplantation  144–5 primary biliary cirrhosis  183, 373 primary graft dysfunction lung (I–R injury)  242–3, 256, 318, 333, 346–7 primary pulmonary hypertension  323–4 prisoners, organ donors  55 professional societies  21 Program-Specific Reports  448 property rights  39, 46 prostacyclin 311 prostaglandin 311, 333 proteasome inhibitor  103–4 protein-losing enteropathy  354 prothrombin time  400 public education  17 pulmonary arterial hypertension  323 pulmonary artery catheters  8, 70, 280, 401–2 pulmonary artery occlusion pressures 402 pulmonary hypertension  227, 232, 307, 309, 361–2 pulmonary vascular disease  323–4, 328–9 pulmonary venous–left atrial anastomoses 329 purine analogues  Q QAPI programme  448 quality control  441 quality improvement  448–9 quality of life  301–2 R rabbit ATG  102 rapamycin (sirolimus)  104–5, 282, 421, 430 rapid infusion systems  9, 410–11 reactive oxygen species  91–2 recovery of organs critical care considerations  73–4 OPO responsibilities  19 red cell alloimmunization  386, 388 red cell irradiation  387–8 red cell storage lesions  386 regulation  10, 13–21, 24–5, 45–6, 447 rejection acute  100, 149, 272, 299, 312, 343–4, 347 chronic 100–1, 149 classification 343, 344 delayed xenograft  113–14 heart transplantation  312, 343–4 history of 3–4 hyperacute 112 immunobiology 99–101 intestinal and multivisceral transplantation 299 lung transplantation  347 non-transplant surgery  421 paediatric heart transplantation  360 paediatric liver transplantation  272 pancreas transplantation  149 remote ischaemic preconditioning  95 renal function elderly 372 heart–lung transplantation  316 intestinal and multivisceral transplantation 297 liver disease  194, 217, 260, 268, 416 liver transplantation  206, 223, 224, 225, 227, 232 lung transplantation  347 neurological death  71 paediatric heart transplantation  360 pancreas transplantation  145 renal replacement therapy  44, 411–16 global context  53 indications 411 intraoperative 415–16 liver disease  217, 416 principles 412 requirements for  412–15 reoxygenation injury  91–2 reperfusion injury  91–3 research, organ donors  77–83 respiratory failure kidney–pancreas transplantation  156–9 kidney transplantation  156–9 liver disease  216–17 pancreas transplantation  156–9 respiratory function elderly patients  371–2 geriatric transplantation  380 liver transplantation  224, 227, 235 neurological death  70 paediatric liver disease  260 paediatric liver transplantation  270 restrictive cardiomyopathy  354–5 restrictive lung disease  321–2, 328, 329 retransplantation liver transplantation  208 paediatric heart transplantation  355 paediatric liver transplantation  265 right ventricular failure  318, 331, 341–2, 361 index right ventricular outflow tract obstruction 329–30 Ringer’s solution  136, 254 risk tolerance  45–6 rituximab 103, 300 ROTEM® (rotational thromboelastometry)  231, 395–400 S Scandiatransplant 25 sedation 217, 270 seizures 271 selling organs  38–9, 53–7 sepsis  153–4, 216, 218, 228, 240, 347–8, 380 septic shock  153–4 serotonin 245–6 seventh day syndrome  243 severe sepsis  153–4 shared agency  40 short bowel syndrome  277, 278, 285 sildenafil 311 sirolimus (rapamycin)  104–5, 282, 421, 430 Six Sigma  449 smoking  127, 133, 206 social worth  48–9 soft presumed consent  21 Southeastern Regional Organ Procurement Program (SEROPP) 13 Southeast Organ Procurement Foundation (SEOPF)  13 Spain 19–20, 34 spontaneous bacterial peritonitis  189 standard acquisition charge  16 standard criteria donors (SCD)  17, 66 standardized protocols  441 statins 378 stem cells  176 steroids  4, 5, 70, 72, 78, 102, 420, 431 steroid-sparing protocols  105 substance misuse  206 suicide right ventricle  330 superoxide 92 suppurative lung disease  322–3, 328, 329, 348 survival hearttransplantation 307 intestinal and multivisceral transplantation 277 kidney transplantation  374–5 liver transplantation  373–4 lung transplantation  321 measurement 48 paediatric heart transplantation  359–60 paediatric lung transplantation  363 Surviving Sepsis Campaign  154 sympathetic storm  69 systemic inflammatory response syndrome (SIRS)  70 T T3 and T4  71–2 tacrolimus  104, 282, 297, 300, 420, 431 T-cell depletion  113 T-cell-mediated acute rejection  100 TEG®  8, 148, 231, 395–400 tetrahydrodeoxycorticosterone 245 TGR  5, 245 thermoregulation 72 thoracic epidural anaesthesia 333, 346 thrombocytopaenia 193 thromboelastography (TEG®)  8, 148, 231, 395–400 thrombotic microangiopathy  298 thymectomy 115 thymic tissue transplantation  112–13 thymoglobulin 102, 421 thymokidney  113, 114, 116 thyroxine 71–2 TIPS 189–91 tissue-specific antigens  99 tissue typing  101 tolerance induction 116–17 strategies 112–13 trafficking in organs  55–7 training 442 tranexamic acid  310–11, 332 transcranial Doppler  63, 403 transfusion-associated circulatory overload 391 transfusion medicine  385–92 transfusion-related acute lung injury  391 transfusion-related immunomodulation 391 transjugular intrahepatic portosystemic shunt (TIPS)  189–91 transoesophageal echocardiography (TOE/TEE) 231, 309, 317, 329–30, 402 transplant tourism  53–7 transpulmonary gradient  308–9 transversus abdominis plane block 255 tromethamine 158 trust 35–6 tuberculosis 240 U UKELD score  201–2 unconsciousness 62 UNetSM 14 Uniform Anatomical Gift Act (2006)  13 United Network for Organ Sharing  14 urinary tract infections  155 usual interstitial pneumonia  321–2, 324 utility 48–50 UW solution  V vaccination, pretransplant  240, 261 valganciclovir 240 varices 186–8 vascularized thymic lobe transplantation 113 vasopressin 70, 71 venovenous bypass  8, 229–30 ventilation ALI/ARDS 157–9 liver disease  216–17 lung transplantation  345 organ donors  70 paediatric liver transplantation 270, 272–3 ventricular assist devices  342, 357 viscoelastic haemostatic assays  395–400 W waiting list management  9, 261, 307 waiting-time based allocation  50–1 warm ischaemia  91 work of breathing  157 X xanthine oxidase  90, 92 xenotransplantation 111–17, 176 advantages 114 bridge to allotransplantation  117 delayed rejection  113–14 donor procedures  115–16 donor selection and preparation  114 donor species choice  111–12 history of 111 immunology 112–14 immunosuppression 112, 116–17 postoperative management  116 pretransplant recipient procedures  115 recipient selection and preparation 114 , 116 renal procurement  116 surgical approach  114–15 thymokidney  113, 114, 116 tolerance induction  116–17 tolerance strategies  112–13 461 .. .Oxford Textbook of Transplant Anaesthesia and Critical Care Oxford Textbooks in Anaesthesia Oxford Textbook of Anaesthesia for the Elderly Patient Edited... Professor of Medicine and Director Division of Hepatology University of Miami Leonard M Miller School of Medicine Miami Florida USA Professor of Clinical Surgery DeWitt Daughtry Department of. .. burgeoning field of transplant anaesthesia and critical care As you will see on initial perusal of the table of contents we have taken a holistic approach to the scope of practice of transplant anaesthesia

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