DSpace at VNU: The 6th meeting of the asian consortium for the conservation and sustainable use of microbial resources (ACM)

DSpace at VNU: The 6th meeting of the asian consortium for the conservation and sustainable use of microbial resources (...

bìa Dear Participants, The exploiting of microbial resource is one of the most concerned problems in the fields of medicine, biotechnology, agriculture, pharmacology… all over the world Asia has been shown to possess extremely abundant and diverse microorganisms, therefore the exchanging and discussing not only scientific problems and ideas but also generally developmental strategies at the common forum for the Asian scientists would be valuable Continuing the annual meeting of Asian Consortium of Microbiology (ACM), this time we are delighted to organize the 6th ACM which will be held on November 27th, 2009 at Hanoi - Vietnam in order to promote collaboration among government or public organizations in Asian countries for the purposes of enhancing conservation and sustainable use of microbial resources in Asia In the proceeding of the 6th ACM the country reports and the keynote lectures as well as some selected scientific articles are present Through the country reports of Vietnam, Thailand, China, Korea, Malaysia and Philippine the activities in bio-diversity conservation, material transfer agreement (MTA) and human resources development will be reviewed Also, four keynote lectures of the invited professors from Japan, Belgium and Vietnam will present specialized topics in the biotechnology field In addition, the short communications, mainly of Vietnamese scientists, will show some of the various aspects to study the bio-diversities and the characteristics of microorganisms Director Dr Duong Van Hop Institute of Microbiology and Biotechnology, Vietnam National University, Hanoi CONTENTS Progam of the 6th ACM Meeting Abstracts of Country Reports Keynote Lectures 19 Short Communications of The Host Country 25 List of Participants 96 Information of Meeting Hall and Hotels 99 Progam of the 6th ACM Meeting Meeting Program November 26th, 2009 Arrival of the participants Moevenpick Hotel, 83A Ly Thuong Kiet, Hanoi November 27th, 2009 Conference room 1, - Moevenpick Hotel 08:30 – 09:00 Registration 09:00 09:30 - Opening Ceremony Dr Duong Van Hop, the director of IMBT - Adoption of 5th ACM Minute of Meeting (Korea) Morning section Chair persons: Dr Duong Van Hop (Vietnam) Dr Jung-Sook Lee (Korea) 09:30 – 10:10 Keynote lecture Genomic approach to seasonal and pandemic influenza viruses Prof Nobuyuki Fujita National Institute of Technology and Evaluation (NITE), Japan Reports from ACM country members (1st part) 10:10 10:25 - Cambodia 10:25 10:40 - China 10:40 – 10:55 - Indonesia 10:55 11:15 Coffee break 11:15 11:55 Keynote lecture From the cells to the end product The case of lactic starter production Prof Phillip Thornart University of Liege, Belgium Lunch 12:00 – 13:30 Chair persons: Dr Sukor Nordin (Malaysia) Dr Juncai Dong (China) Reports from ACM country members (2nd part) - Japan - Korea - Malaysia Afternoon section 13:30 13:45 13:45 – 14:00 14:00 – 14:15 14:15 – 14:55 Keynote lecture Deciphering On-Off signaling network of Streptomyces secondary metabolism Prof Takuya Nihira International Center for Biotechnology, Osaka University, Japan 14:55 – 15:10 15:10 – 15:50 15:50 – 16:05 16:05 – 16:20 16:20 – 16:35 18:00 – 21:00 Coffee break Keynote lecture Yeast biodiversity and application in biotechnology – Research at FIRI Vietnam Dr Vu Nguyen Thanh Food Industrial Research Institute (FIRI) - Vietnam Reports from ACM country members (3rd part) - The Philippines - Thailand - Vietnam Dinner, Sen Resaurant, West Lake - Hanoi Novotel Halong Bay November 28th, 2009 08:00 – 12:00 Travel to Halong 12:00 – 14:00 - Check-in at Novotel Halong Bay - Lunch Scientific disscusion Yen Tu Conference room Chair persons: Dr Suzuki Ken-Ichiro (Japan) Dr Tanit Changthavorn (Thailand) 14:00 – 16:30 Report on Task Force: - Biological Information Management (BIM) - Human Resource Development (HRD) - Management of Material Transfer (MMT) General Discussion Conclusion Announcement of 7th and 8th ACM meeting Closing Address 18:00 – 21:00 Dinner, The Square Restaurant, Hovotel Halong Bay November 29th, 2009 08:00 – 08:30 Hotel check-out 09:00 – 15:00 Boat tour in Halong Bay 15:00 – 19:00 Travel back to Hanoi November 30th, 2009 Departure of the participants Abstracts of Country Reports Yuguang Zhou, 6th ACM meeting – Hanoi 2009 Country Report from China Yuguang Zhou* Institute of Microbiology, Chinese Academy of Sciences, NO.1 West Beichen Road, Chaoyang District, Beijing 100101, China ABSTRACT Biological resource centers (BRCs) are an essential part of the infrastructure dedicated to the investigation of the structure, functions, and applications of biological systems by developing cooperative activities for sharing validated biological material and data among government agencies, industry, academia, and the public Some attempts have been made since 1980’s for improving culture collection management in China including establishment of CCCCM, CTCCCAS and NFSNR China General Microbiological Culture Collection Center (CGMCC), founded in 1952, is affiliated with the Institute of Microbiology of the Chinese Academy of Sciences (IMCAS), which is a leading microbiological research institution in China The CGMCC became a member of the CCCCM, CTCCCAS and World Federation for Culture Collections (WFCC) in the early 80’s or 90’s and acquired the status of an International Depositary Authority (IDA) under the Budapest Treaty Biological Resources Center, Institute of Microbiology Chinese Academy of Sciences (IMCASBRC) has been set up by integrating the China General Culture Collection Center (CGMCC), the Information Center and the newly developed high throughput evaluation platform of microbial metabolites in July, 2009 The mission of IMCAS-BRC is to serve as an integrated public infrastructure of culture collection, research and utilization of microorganisms, in order to sustain biotechnology innovation IMCAS-BRC not only provides universities, institutions, and * Corresponding author Tel.: 86-10-64807355 E-mail: zhouyg@im.ac.cn 10 LTH Yen et al., 6th ACM meeting – Hanoi 2009 Mycologycal diversity and biological activities of soil fungi isolated in Trung Khanh nature reserve Le Thi Hoang Yen1,*, Nguyen Hong Minh1, Duong Van Hop1, Nguyen Lan Dung1, Katsuhiko Ando2 Viet Nam Type Culture Collection, Institute of Microbiology and Biotechnology, Vietnam National University, Hanoi, 144 Xuan Thuy, Hanoi National Institute of Technology and Evaluation (NITE), Japan Abstract From 20 soil samples which were collected in Trung Khanh Nature Reserve in Vietnam 221 isolates of fungi were investigated, 87 fungi were identification in to species level, they were belonging to 15 genera: Acremonium, Arthrographis*, Aspergillus, Chlotridium, Cunninghammella, Fusarium, Gliocladium, Gongroniella*, Isthmologispora*, Metarrhizium, Orchroconis*, Paecilomyces, Penicillium, Torula, Trichoderma Among them genera has not been reported in Vietnam (the genera with* mark) All of those fungi were examined antimicrobial activity and endo-β glucanase and α- amylase activity Six genera of Aspergillus, Arthrographis, Fusarium, Paecilomyces, Penicillium, Trichoderma were showed two enzyme activities, and each of genera has antimicrobial activity (against to 1-3 test strains) Keyword: Diversity, soil fungi in Viet Nam Introduction The measurement of fungal diversity has received increased attention over the last decade, in part due to the fact that fungi have the potential for industrail and biotechnological applications (Hawksworth, 1983; Lodge, 1997), such as antibiotic and enzyme production, food processing, being agent in biopulping paper even in bioremediation of chemical spills (Bogan et al.,1996) Therefore, fungi has been screening for diversity as well as discorvery of the rare but valuable species (Hyde, 2001) * Corresponding author Tel.: +84-1688568454 E-mail: yenlth@vnu.edu.vn 94 LTH Yen et al., 6th ACM meeting – Hanoi 2009 Hawksworth (1990) estimated that the fungal population globally is about 1.5 million According to dictionary of fungi (Ainsworth, 1995), fungi kingdom has 7379 genera, 76360 species About 40000 species was reported in China, but only 232 genera, 785 species were reported in Vietnam So the knowledge of the fungal population in Vietnamese tropical forests as well as of their roles is very significant to biodiversity accessing This research atempted to measure the microfungal population on 20 soil samples in Trung Khanh National Forest Besides, antimicrobial activity and enzyme production activities of these fungi were also studied Materials and methods 2.1 Samples: 20 soil samples were collected in Trung Khanh National Forest 2.2 Isolation and identification method Soil dilution plate method: The soil samples were mixed with sterilized water and a series of dilutions was made From the dilutions, 0.1ml was pippeted on to PDA and LCA and incubated in 28 °C within one week Fungal colonies were then picked and transferred to new media Identification based on fungal morphological characteristics was performed according to Carmichael (1980), Ellis (1971), Gams (1993, 2006), Raper and Fennel (1968), Samson (2004), Raper and Fennell (1965) Phylogeny analysis: Total DNA of fungal was extracted by Peterson’s method PCR amplification employed a pair of universal primers, NL1 and NL4 (Peterson, 2004) Direct sequencing was performed on the Applied Biosystems 3100 DNA analyzer and with BigDye terminator v3.1 cycle sequencing Kit (QAGen) For cycle sequencing reactions, primer NL1/NL4 were used Phylogenetic analysis was conducted by the Clustal X and Neighborjoining method Onygenales species were added in analysis as outgroup: AB027382.1 Beauveria bassiana Support for individual nodes was assessed by bootstrap analysis, resembling the datasets 1.000 times 2.3 Biological activity: Fungi strains were cultivated on to PDA media, 28 °C for weeks for enzyme and antimicrobial activity Screening of microbes producing endo-β-glucanase and α-amylase activity 95 LTH Yen et al., 6th ACM meeting – Hanoi 2009 The enzyme activity of fungi was estimated by putting peace of week fungal culture on to the medium contained 1% CMC (or 1% soluble starch), 18% agar-agar, then incubated in 28 °C After 24 hours, the plates were stained with Congo red 1% (or Lugol’s solution) followed by decolorization with M sodium chloride, the halo can be visualized in the background of redstained for CMC (or iodine stained for amylase) The visualized halos were detected, and the fungi displayed visualized halo was considered as positive one Screening of fungi producing antimicrobial activity The antimicrobial activity of fungi was estimated by putting one peace of week fungal culture on to (NA or PDA) medium contain test strains The test strains were microorganisms obtained from VTCC: Candida albicans VTCCY-674; AE: Escherichia coli VTCCB-883; Bacillus subtilis VTCCB-888; Fusarium oxysporum VTCCF-0068 After incubation at 37 °C, 24 hours if test strains were bacteria and 28 °C, 10 days if test strains were fungi, the inhibition zone were determined, and samples displaying an inhibition zone were considered as positive Results and discussion 3.1 Mycological diversity From 20 soil samples, a total 32 fungal taxa were collected (Table 1), consisting of 27 Ascomycete (representing of 84.6% of all mycota in this work) and mitosporic fungi (15.6%) About 26% (4/15 genera) of the fungi were previously not reported in Vietnam (Bui Xuan Dong et al., 2001; Nguyen Thi Lien Hoa et al., 2007; Nguyen Thi Mien et al.; 2005) Most of Ascomycete species belonged to the families: Hypocreaceae (35%), Trichocomaceae (53%), the others belonged to Eremomycetaceae, Mucoraceae, Cunninghamellaceae (Fig.1) Nine genera of Ascomycete were identified in this study, and Aspergillus is the most common genus with species, after that is Penicillium with species, next is Trichoderma, Fusarium, Paecilomyces with species each genus (Table 1) The other genera with low frequency (5%) in this research (Arthrographis, Gongronella) were not reported in Vietnam before Five genera of mitosporic fungi were identified in this study and the genus Metarhizium is the most common genus with the frequency of occurrence of the species Metarhizium anisopliae is 40% (Table 1) The genus Isthmologispora well represented in aquatic litter fungi but appeared in this work, was also not reported in Vietnam The mitosporic fungi Ochroconis was not reported in Vietnam, too 96 LTH Yen et al., 6th ACM meeting – Hanoi 2009 Table1 Frequency of occurrence of soil fungi isolated in Trung Khanh Nature Reserved Frequency of occurrence (%)1 No Family Species name Eremomycetaceae Arthrographis kalrae Cunninghamellaceae Cunninghamella echinulata Mucoraceae Gongronella butleri Hypocreaceae Acremonium murorum 10 Acremonium sp 30 Fusarium oxysporum 35 Fusarium proliferatum 10 Fusarium solani 25 Trichoderma koningii 10 Trichoderma pseudokoningii 11 Trichoderma viride 12 Gliocladium penicillioides Paecilomyces lilacinus 60 14 Paecilomyces carneus 20 15 Penicillium sclerotiorum 10 16 Penicillium multicolor 20 17 Penicillium commune 20 18 Penicillium marneffei 20 19 Aspergillus tubingensis 20 Aspergillus niger 30 21 Aspergillus terreus 10 22 Aspergillus versicolor 23 Aspergillus puniceus 24 Aspergillus oryzae 25 Aspergillus flavus 26 Eurotium amstelodami 27 Aspergillus carneus Chloridium schulzeri 29 Metarhizium anisopliae 40 30 Ochroconis humicola 31 Isthmologispora ampulliformis 10 32 Torula humicola 13 28 Trichocomaceae Fungi imperfecti Frequency of occurrence (%) = (number of samples on which a given taxon occurred/ number of samples examined) x 100 97 LTH Yen et al., 6th ACM meeting – Hanoi 2009 Fig.1 Ascomycete families encountered 3.2 Biological Activities All of 87 fungal taxa were examined their biological activities of secondary metabolites by testing antimicrobial activity and endo-β glucanase and α-amylase activity The results are showed in Table The positive strains of endo-β glucanase and α-amylase activity were belonged to six genera: Aspergillus, Arthrographis, Fusarium, Paecilomyces, Penicillium, Trichoderma Five of these genera (Aspergillus, Fusarium, Paecilomyces, Penicillium, Trichoderma) are common fungus in tropical areas and they are used for many industrial and biotechnological applications (Hawksworth, 1990; Lodge, 1997), such as antibiotic and enzyme production, food processing, beeing agent in biopulping paper These six genera also had antimicrobial activity against test fungus In this study, the genus Paecilomyces was the strongest one showing antibacterial activity with 8/14 (64%) strains against Escherichia coli (Fig 2) and Bacillus subtilis Beside that, the genus Trichoderma also showed high potential in antibacterial activity with 4/7 (57%) strains More ever, these genera showed the antifungal activity against Fusarium oxysporum (Fig 4) Among 22 strains of the genus Aspergillus, two strains had antibacterial activity against Bacillus subtilis and one isolate showed antifungal activity against Fusarium oxysporum Penicillium genus was regarded as one of the most famous genera with antimicrobial activity In this study it had antibacterial activity against both Escherichia coli and Bacillus subtilis In this research, among 87 fungal strains isolated there is only one Fusarium strain had antiCandida albican activity (Fig 3) The Arthrographis genus had low frequency of occurrence in diversity, but found the antibacterial activity against Bacillus subtilis 98 LTH Yen et al., 6th ACM meeting – Hanoi 2009 Table Biological activities of Trung Khanh soil fungi The fungal genera No of strains Number of positive strains CMC AM AC AE AB AF Arthrographis 1 0 Aspergillus 22 2 0 Fusarium 16 2 Paecilomyces 14 4 4 Penicillium 1 Trichoderma 2 1 Chloridium 0 0 0 Metarhizium 0 0 0 Isthmologispora 0 0 0 Torula 0 0 0 Acremonium 0 0 0 Gongronella 0 0 0 Cunninghamella 0 0 0 0 0 0 Gliocladium CMC: CMCase activity; AM: α- Amylase activity; AC: Anti Candida albican activity; AE: Anti Escherichia coli; AB: anti Bacillus subtilis; AF: anti Fusarium oxysporum Fig Anti Bacillus subtilis activity Fig Anti Candida albican activity 99 LTH Yen et al., 6th ACM meeting – Hanoi 2009 Fig Anti Fusarium oxysporum activity 3.3 Phylogenetic analyse of three Aspergillus species showing high biological activities 0.02 Aspergillus proliferans Eurotium tonophilum Eurotium carnoyi Eurotium herbariorum Eurotium chevalieri 728 Eurotium repens Eurotium intermedium 739 CB83_N1_4 Eurotium amstelodami Eurotium cristatum 1000 Eurotium rubrum 736 Eurotium pseudoglaucum Eurotium medium Eurotium echinulatum 765 Eurotium athecium Eurotium niveoglaucum Aspergillus westerdijkiae Aspergillus candidus 999 Aspergillus allahabadii Fennellia nivea 1000Aspergillus terreus Aspergillus janus Aspergillus clavatus 998 Aspergillus flavipes Aspergillus.iizukae 1000 Fennellia flavipes Aspergillus aureofulgens Aspergillus carneus 990 CB05_N1_1 1000 Aspergillus egyptiacus 788 Aspergillus granulosus Aspergillus puniceus 998 pseudodeflectus 762 Aspergillus Aspergillus ustus Aspergillus caesiellus 940 Aspergillus sydowii 954 Aspergillus versicolor 1000 CB62_N1_2 AB027382.1 Beauveria bassiana Fig NJ-tree of three Aspergillus species: Aspergillus versicolor; Aspergillus carneus; Eurotium amstelodami The tree was run with the LSU-rDNA Values of the node are bootstrap values calculated from 1000 replications Bar, 0.02 Knuc 100 LTH Yen et al., 6th ACM meeting – Hanoi 2009 Recently fungal taxonomy is based on morphological and physiological similarities In this study, most of fungal strains were identified based on morphological characteristics To test the current phenotype-based classification systems, the D1D2 regions of the large subunit ribosomal RNA (LSU-rDNA) gene of three strains of Aspergillus genus which showing high antimicrobial activities: CB005- Aspergillus carneus, CB062- Aspergillus versicolor; CB083- Eurotium amstelodami were sequenced Data were analyzed by Clustal X and NJ-method The result was shown in Fig Aspergillus strains were assigned to three branches, based on clades supported by high bootstrap values Apical regions of the tree were generally well supported in bootstrap analysis, but basal regions of the resulting tree were not The NJ-tree has compatible with phynotype- based identification was given Therefore, in the context of Aspergillus, the majority of the species accepted by Raper and Fennell (1965) could be distinguished by differences in the DNA sequence from D1D2 regions Conclusion - From 21 soil samples collected in Trung Khanh Nature Reserve, 221 fungal strains were isolated - Eighty seven fungal strains were identified in to species level they belonged to 15 genera, among them genera were not reported in Viet Nam (Arthrographis, Gongroniella, Isthmologispora, Orchroconis) - Six genera: Aspergillus, Arthrographis, Fusarium, Paecilomyces, Penicillium, Trichoderma were not only widely spread fungi but showed high antimicrobial and enzyme activities - Aspergillus genus could be distinguished based on morphology characteristics or DNA sequence from D1D2 regions analyze Acknowledgements This study was supported partly by special coordination funds of “Conserve and maintain microbial resources” - the independent project of Misnistry of Sceince and TechnologyVietnam 101 LTH Yen et al., 6th ACM meeting – Hanoi 2009 Reference: [1] Bui Xuan Dong, Trinh Tam Kiet (2001), List of Plantarum in Viet Nam, Viet Nam Agriculture Academic Publisher [2] BW Bogan, RT Lamar (1996), “Polycyclic aromatic hydrocarbon-degrading capabilities of Phanerochaete laevis HHB-1625 and its extracellular ligninplytic enzymes”, Appl Environ Microbiol 62 pp 1597 – 1603 [3] DJ Lodge (1997),“Factors related to diversity of decomposer fungi in tropical forests”, Biological Conservation, pp 681 – 688 [4] DL Hawksworth (1983), “A key to the lichen-forming, parasitic, parasymbiotic and saprophytic fungi occurring on lichens in the British Isles”, Lichenologist, 15 pp – 44 [5] DL Hawksworth, PM Kirk, BC Sutton, DN Pegler (1995), Ainsworth & Bisby's Dictionary of the Fungi, CAB International [6] KB Raper, C Thom (1968), A manual of the Penicillia, Hafner Publishing Company, New York and London [7] KD Hyde, DQ Zhou (2001), “Host-specificity, host- exclusivity and host- recurrence in saprobic fungi”, Myco Res., 105 pp 1449 – 1457 [8] KH Domsch, W Gams (1993), Compendium of soil fungi, IHW-Verlag [9] MB Ellis (1971), Dematiaceus Hyphomycetes, Commonwealth Mycological Institute Kew, Surrey, England [10] Nguyen Thi Lien Hoa (2007), Report on taxonomy of micro fungi in and surround Ho Chi Minh Tomb, N0 69 Institute Report [11] Nguyen Thi Mien (2005), List of filamentous fungi isolated in Viet Nam [12] S Peterson (2000), “Phylogenetic relationship in Aspergillus base on rDNA sequence analysis”, in the Integration of modern taxonomic methods for Penicillium and Aspergillus classification, Harwood Academic Publisher [13] W Gam (2006), Hypocrea and Trichoderma studies marking the 90th birthday of Joan M.dingley, Centraalbureau voor Schimmelcultures, Utrecht, the Netherlands, An institute of the Royal Nertherlands Academy of Arts and Sciences 102 Miscellaneous 103 List of Participants No Country Cambodia China China China Name Oum Pisey Dr Juncai Dong Dr Xiuzhu Dong Yuguang Zhou Title / Institution Tel./Email Secretariat of National Tel +855-23-217560 Biosafety Steering Committee Fax +855-23-217560 Ministry of Environment piseyoum@hotmail.com Institute of Microbiology, Chinese Academy of Sciences Institute of Microbiology, Chinese Academy of Sciences Institute of Microbiology, Chinese Academy of Sciences Biotechnology Development Japan Dr Ando Katsuhiko Center (NBDC), National Institute of Technology and Evaluation (NITE) NITE Biological Resource Japan Dr.Kawasaki Hiroko Center (NBRC), National Institute of Technology and Evaluation (NITE) NITE Biological Resource Japan Dr Suzuki Ken-Ichiro Center (NBRC), National Institute of Technology and Evaluation Tel 86-10-64807422 Fax 86-10-64807422 ma@im.ac.cn Tel 86-10-64807413 Fax 86-10-64807413 dongxz@im.ac.cn Tel 86-10-64807355 Fax 86-10-64807288 zhouyg@im.ac.cn Tel 81-438-20-5760 Fax 81-438-20-5766 ando-katsuhiko@nite.go.jp Tel 81-438-20-5763 Fax 81-438-52-2329 kawasaki-hiroko@nite.go.jp Tel 81-438-20-5763 Fax 81-438-52-2329 suzuki-ken-ichiro@nite.go.jp Deputy Director-General Japan Dr Fujita Nobuyuki Department of Biotechnology Tel 81-438-20-5760 (DOB), National Institute of Fax 81-438-20-5766 Technology and Evaluation fujita-nobuyuki@nite.go.jp (NITE) Biotechnology Development 104 Japan Mr Shioya Shun Center (NBDC), National Tel 81-438-20-5760 Institute of Technology and Fax 81-438-20-5766 Evaluation (NITE) shioya-shun@nite.go.jp 10 Japan Prof Takuya Nihira (and Chizuko Nihira) International Center for Tel 81-6-6879-7452 Biotechnology, Osaka Fax 81-6-6879-7454 University nihira@icb.osaka-u.ac.jp Tel 82-42-860-4600 11 Korea Jung-Sook Lee KCTC/BRC/KRIBB111 Fax 82-42-860-4625 jslee@kribb.re.kr Tel 82-42-860-4624 12 Korea Kee-Sun Shin KCTC/BRC/KRIBB111 Fax 82-42-860-4625 ksshin@kribb.re.kr Bioresource Management and 13 Malaysia Dr Tan Geok Hun Utilization Programme, Strategic Resource Research Centre, MARDI 14 Malaysia Tel 603-89437439 Fax 603-89437677 tangh@mardi.gov.my Dr Sukor Nordin Philippine National Collection of Microorganism (PNCM) 15 The Philippines Dr Rosario G Monsalud National Institute of Molecular Biology and Biotechnology University of the Philipines at Tel 63 (049)536-2884 Fax 63 (049)536-2884 rgm_pncm@yahoo.com Los Banos College National Institute of Molecular 16 The Philippines Biology & Biotechnology Ms Precy M Rasco (BIOTECH) pmrasco@yahoo.com University of the Philippines Los Baños College 17 18 19 20 Thailand Thailand Thailand Thailand Dr Supawadee Ingsriswang Ms.Pariyaporn Thengprasert Dr Tanit Changthavorn Mrs Wanchern Potacharoen National Center for Genetic Tel (66-2) 564 6700 Engineering and Fax (66-2) 564 6701-5 Biotechnology (BIOTEC) supawadee@biotec.or.th National Center for Genetic Tel (66-2) 564 6700 Engineering and Fax (66-2) 564 6701-5 Biotechnology (BIOTEC) pariyaporn.the@biotec.or.th National Center for Genetic Tel (66-2) 564 6700 Engineering and Fax (66-2) 564 6701-5 Biotechnology (BIOTEC) tanit@biotec.or.th National Center for Genetic Tel (66-2) 564 6700 Engineering and Fax (66-2) 564 6701-5 Biotechnology (BIOTEC) wanchern@biotec.or.th 105 21 22 Vietnam Vietnam Prof Nguyen Lan Dung Dr Duong Van Hop Institute of Microbiology and Biotechnology (IMBT) Institute of Microbiology and Biotechnology (IMBT) Université de Liège, 23 Centre Wallon de Biologie Belgium Prof Ph Thonart Industrielle Boulevard du Rectorat 106 Tel 84 972 1815 Fax 84 37547407 dungnguyenlan@yahoo.com Tel 84 7547695 Fax 84 37547407 vanhop93@yahoo.com Tel.: +32.4.366.28.61 p.thonart@ulg.ac.be Information of Meeting Hall and Hotels Mövenpick Hotel Meeting hall: Conference room 1, Address: 83A Ly Thuong Kiet, Hoan Kiem District, Hanoi City, Vietnam Telephone Number: +84 38 22 28 00 Fax: +84 38 22 28 22 E-mail: hotel.hanoi@moevenpick.com Homepage: www.moevenpick-hanoi.com 107 Novotel Ha Long Bay Meeting hall: Yen Tu Conference room Address: Ha Long Road, Bai Chay Ward, Halong City, Quangninh Province, Vietnam Telephone Number: +84 (0) 33 3848 108 Fax: +84 (0) 33 3696 808 E-mail: info@novotelhalong.com.vn Homepage: http://www.novotelhalongbay.com/ 108 ... The 6th Meeting of the Asian Consortium for the Conservation and Sustainable Use of Microbial Resources (ACM) , Institute of Microbiology and Biotechnology (IMBT) and Vietnam National University... collaboration among government or public organizations in Asian countries for the purposes of enhancing conservation and sustainable use of microbial resources in Asia In the proceeding of the 6th. .. Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure A few meeting have been carried out to discuss the matter for joining the treaty