DSpace at VNU: Volatile Constituents of the Leaf Oil of Alchornea tiliifolia (Benth.) Muell. (Family Euphorbiaceae) from Vietnam

This article was downloaded by: [Thammasat University Libraries] On: 08 October 2014, At: 18:10 Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK Journal of Essential Oil Research Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/tjeo20 Volatile Constituents of the Leaf Oil of Alchornea tiliifolia (Benth.) Muell (Family Euphorbiaceae) from Vietnam a b b Nguyên Anh Dũng , Trân Đình Thăng , Hoàng Văn Lų'u & Nguyên Xuân Dũng a Faculty of Biology , Vinh University , 182, Le Duan, Vinh, Vietnam b Faculty of Chemistry , Vinh University , 182, Le Duan, Vinh, Vietnam c c Faculty of Chemistry , College of Natural Science, Hanoi National University , 19-Le Thanh Tong, Hanoi, Vietnam Published online: 09 Dec 2011 To cite this article: Ngun Anh Dũng , Trân Đình Thăng , Hồng Văn Lų'u & Nguyên Xuân Dũng (2009) Volatile Constituents of the Leaf Oil of Alchornea tiliifolia (Benth.) Muell (Family Euphorbiaceae) from Vietnam, Journal of Essential Oil Research, 21:1, 1-2, DOI: 10.1080/10412905.2009.9700091 To link to this article: http://dx.doi.org/10.1080/10412905.2009.9700091 PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content This article may be used for research, teaching, and private study purposes Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden Terms & Conditions of access and use can be found at http:// www.tandfonline.com/page/terms-and-conditions J Essent Oil Res., 21 (January/February 2009) Volatile Constituents of the Leaf Oil of Alchornea tiliifolia (Benth.) Muell (Family Euphorbiaceae) from Vietnam ˜ Anh Dung, Nguyˆen ˜ Faculty of Biology, Vinh University, 182- Le Duan, Vinh, Vietnam Tran ˆ Ðình Thang ˘´ and Hoàng Van ˘ Lu’u, · Downloaded by [Thammasat University Libraries] at 18:10 08 October 2014 Faculty of Chemistry, Vinh University, 182- Le Duan, Vinh, Vietnam ˜ Xuân Dung,* Nguyˆen ˜ Faculty of Chemistry, College of Natural Science, Hanoi National University, 19-Le Thanh Tong, Hanoi, Vietnam Abstract The leaf oil of Alchornea tiliifolia (Benth.) Muell produced from plants growing in Pu Mat National Park, Nghean province, Vietnam was prepared by steam distillation The oil was analyzed by GC and GC/MS A total of 45 compounds were identified, of which b-pinene (31.4%), linalool (17.0%) and b-caryophyllene (10.7%) were found to be major components Key Word Index Alchornea tiliifolia, Euphorbiaceae, essential oil composition, b-pinene, linalool, b-caryophyllene Introduction Experimental The Euphorbiaceae family consists of about 317 genera and 5000 species which are widespread, primarily in the tropics and subtropics, poorly represented in temperate regions (1) About 70 genera and 460 species are distributed in Vietnam (2) Many species of Euphorbiaceae are of economic importance, such as sources of medicine, provisions, paint, rubber, insecticides, etc (1,3) Alchornea tiliifolia (Benth.) Muell (Local name: Dom dom la day) (syn Stipellaria tiliifolia Benth.) is one of the 60 species belong to the genus Alchornea, distributed in Son La, Cao Bang, Quang Ninh, Bac Can, Tuyen Quang, Phu Tho, Ha Tay provinces, Vietnam (2); India, China, Laos, Cambodia, Malaysia and Thailand (3) In the course of the systematic study of Alchornea species in Indochina, mono-, sesquiterpenes and other components of the leaf oil of A tiliifolia from Nghean province, Vietnam have been investigated Source: Alchornea tiliifolia (Euphobiaceae) is a shrub tree that can grow up to 1–3 m high in Vietnam Leaves were collected in Pu Mat National Park, Nghean province A voucher specimen (DD4) has been deposited in the Herbarium of the Institute of Ecology and Biological Resources, Vietnamese Academy of Science and Technology Fresh leaves were shredded and their oil was obtained by steam distillation for h at normal pressure, according to the Vietnamese Pharmacopoeia (4) The yield of the fresh leaf oil was 0.22% GC: About 15 mg of oil, which was dried over anhydrous sodium sulfate, was dissolved in mL of CH3OH (for spectroscopy or for chromatography) GC analysis was performed on a HP 6890 Plus Gas chromatograph equipped with a FID and fitted with a 25 m x 0.25 mm x 0.25 mm film thickness HP-5MS column The analytical conditions were: carrier gas H2 with flow rate 35 cm/min, injec- Received: February 2005 *Address for correspondence Revised: December 2006 1041-2905/09/0001-01$14.00/0­—© 2009 Allured Business Media Vol 21, January/February 2009 Accepted: December 2006 Journal of Essential Oil Research/1 Dung ˜ et al tor temperature (PTV) 250°C, detector temperature 260°C, temperature programmed 60°C (2 hold) to 220°C (10 hold) at 4°C/min The relative percentage amounts of the separated compounds were computed from FID data without the use of correction factors GC/MS: A Hewlett Packard 6890 Plus Chromatograph was fitted with the same fused silica HP-5MS capillary col- lumn The conditions of use were the same as described above except that He was the carrier gas with flow rate 30 mL/min, and there was an interface with a mass spectrometer HP 5972 MSD (70 eV) The temperature was programmed as reported above Component identification was carried out by comparing MS data with those reported in Wiley Library on Chemstation HP, and in some cases substances identified from oils known composition and also with standard substances (5–10) Results and Discussion Table I Percentage composition of the leaf oil of Alchornea tiliifolia from Vietnam Downloaded by [Thammasat University Libraries] at 18:10 08 October 2014 Compound RI a-thujene 931 a-pinene 939 camphene 953 sabinene 976 b-pinene 980 myrcene 990 a-terpinene 1019 p-cymene 1028 limonene 1032 1,8-cineole 1034 (Z)-b-ocimene 1042 (E)-b-ocimene 1053 g-terpinene 1061 terpinolene 1090 linalool 1100 nonanal 1104 a-fenchol 1112 borneol 1167 terpinen-4-ol 1179 a-terpineol 1191 myrtenol 1195 neryl formate 1267 a-copaene 1378 b-cubebene 1392 a-cedrene 1411 b-caryophyllene 1419 b-gurjunene 1434 a-humulene 1454 (E)-b-farnesene 1459 allo-aromadendrene 1460 g-curcumene 1480 germacrene D 1480 (Z,E)-a-farnesene 1484 a-muurolene 1500 (E,E)-a-farnesene 1509 bicyclogermacrene 1506 (Z)-g-bisabolene 1515 d-cadinene 1525 (E)-g-bisabolene 1533 a-calacorene 1542 nerolidol* caryophyllene oxide 1581 bisabolol oxide B 1619 g-eudesmol 1630 farnesol* % FID 0.4 8.4 0.2 1.5 31.4 0.9 0.3 0.4 1.2 0.5 trace 0.2 1.2 0.3 17.0 0.1 trace trace 0.8 0.5 0.1 0.3 1.4 0.3 trace 10.7 0.5 1.5 1.1 0.2 0.5 0.7 0.3 0.1 2.3 4.3 0.4 0.3 0.1 0.2 trace 1.4 0.1 0.11 5.9 The leaf oil of A tiliifolia from Vietnam, obtained in 0.22% yield was composed entirely of mono- and sesquiterpenes Forty-five components were identified in the oil (Table I) The main components were b-pinene (31.4%), linalool (17.0%) and b-caryophyllene (10.7%) These compounds accounted for 59.1% of the oil These main compounds and other oxygenated components such as 1,8-cineole, nonanal, a-fenchol, borneol, terpinen-4-ol, a-terpineol, myrtenol, neryl formate, nerolidol, caryophyllene oxide, bisabolol oxide B, eudesmol and farnesol contribute to the characteristic aroma of the leaf oil Other less predominant compounds were a-pinene (8.4%), a-bisabolene (4.3%) and farnesol (5.9%) To the best of our knowledge until now no publication on chemical composition of the essential oil of this species has been published Acknowledgments The authors wish to thank Vu Xuan Phuong, Institute of Ecology and Biological Resources, Vietnamese Academy of Science and Technology for confirmation of the plant identity and Pu Mat National Park for help collecting samples References Z.G Wu and P.P.H Raven (Eds.), Flora of China Vol 11 (Oxalidaceae through Aceraceae) Science Press, Beijing, China and Missouri Botanical Garden Press, St Louis, MO (in preparation 2005) N.T Ban (Ed.), Checklist of Plant Species of Vietnam Agricultural Publishing House, Hanoi, Vietnam (2003) R Smith, Genera Euparbiacearum (Royal Botanic Gardens, Kew) (2001) Vietnamese Pharmacopoeia Medical Publishing House, Hanoi, Vietnam (1997) S.R Heller and G.W.A Milne, EPA/NIH Mass Spectral Data Base U.S Government Printing Office, Washington, DC (1978,1980,1983) E Stenhagen, S Abrahamsson and F.W McLafferty, Registry of Mass Spectral Data Wiley, New York, NY (1974) A Swigar and R.M Siverstein, Monoterpenens Aldrich, Milwaukee, WI (1981) R.P Adams, Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectrometry Allured Publ Corp., Carol Stream, IL (2001) D Joulain and W.A Koenig, The Atlas of Spectral Data of Sesquiterpene Hydrocarbons E.B Verlag, Hamburg, Germany (1998) 10 N.X Dung, P.H Ngoc, D.D Rang, N.T Nhan, N.Klinkby and P.A Leclercq, Chemical Composition of the Volatile Concentrate from the Flowers of Vietnamese Alstonia scholaris (L.) R., Br., Apocynaceae J Essent Oil Res., 13, 424–426 (2001) trace < 0.1%; * correct isomer not identified 2/Journal of Essential Oil Research Vol 21, January/February 2009 ... Essent Oil Res., 21 (January/February 2009) Volatile Constituents of the Leaf Oil of Alchornea tiliifolia (Benth.) Muell (Family Euphorbiaceae) from Vietnam ˜ Anh Dung, Nguyˆen ˜ Faculty of Biology,... course of the systematic study of Alchornea species in Indochina, mono-, sesquiterpenes and other components of the leaf oil of A tiliifolia from Nghean province, Vietnam have been investigated... ˜ Faculty of Chemistry, College of Natural Science, Hanoi National University, 19-Le Thanh Tong, Hanoi, Vietnam Abstract The leaf oil of Alchornea tiliifolia (Benth.) Muell produced from plants