ATOMIC ABSORPTION SPECTROSCOPY

ATOMIC ABSORPTION SPECTROSCOPY (AAS) LE THI THAO NGUYEN 1101760017 APPLICATION • CONCLUSION • • TECHNIQUE • INTRODUCTION CONTENTS • INTRODUCTION Monochromator Detector Lamp Flame Atomic absorption spectroscopy • INTRODUCTION Excited State   E Excitation Ground State Emission E1 E0 • TECHNIQUE Principle Flame Atomizer Detector & Recorder Hollow Cathode Lamp Monochromator Nebulizer Sample Hollow Cathode Lamp Each element has its own unique lamp Nebulizer Monochromatic It creates a fine spray To select the specific wavelength of light of the sample The selection of the specific wavelength allows the determination of the element Atomizer It breaks down the molecules into the atoms Detector To convert the light signal to electrical signal proportional to the light intensity 2 • TECHNIQUE Notices Sample (solution) HCl < HNO3 < H2SO4 < H3PO4 < HF • TECHNIQUE Determination Calibration curve method A=K.C STEP • A: intensity K: constant Prepare standard samples with different determined concentrations (C1,C2,C3,C4,C5…) C Concentration of element in sample • Measuring intensity of absorption of each standard samples (A1,A2,A3,A4,A5…) • Draw a graph indicate the relationship between concentration and intensity: y=ax Intensity STEP y=ax Concentration • • Measure times intensity of real samples Determine the concentration of real sample by using calibration curve 3 • APPLICATION Determine Ca2+ in hard water Prepare standard samples 422.7 Wavelength (nm) Real Sample Evaporate the liquid sample, until obtain m(g) solid Dissolve m(g) solid in hydrochloride acid (1%) • APPLICATION Flame Atomizer Detector & Recorder Hollow Cathode Lamp Monochromator Nebulizer Absorption intensity Sample f(x) = 4.75x + 0.01 R² = Concentraton (μg/ml) Standard sample Absorption intensity • APPLICATION f(x) = 4.75x + 0.01 R² = •   Absorption of real sample is 0.75 (A=0,75) • 0.75 0.75 -> X (C real sample) = 0.156 (μg/ml) • Concentraton (μg/ml) Use graph to determine concentration of real sample • CONCLUSION ... • • TECHNIQUE • INTRODUCTION CONTENTS • INTRODUCTION Monochromator Detector Lamp Flame Atomic absorption spectroscopy • INTRODUCTION Excited State   E Excitation Ground State Emission E1 E0 •... Monochromator Nebulizer Absorption intensity Sample f(x) = 4.75x + 0.01 R² = Concentraton (μg/ml) Standard sample Absorption intensity • APPLICATION f(x) = 4.75x + 0.01 R² = •   Absorption of real... concentrations (C1,C2,C3,C4,C5…) C Concentration of element in sample • Measuring intensity of absorption of each standard samples (A1,A2,A3,A4,A5…) • Draw a graph indicate the relationship between