Evaluation of the in vivo analgesic and anti inflammatory activities of 80% methanol extract of leonotis ocymifolia (burm f ) iwarsson leaves

Evaluation of the in vivo analgesic and anti-inflammatory activities of 80% methanol extract of Leonotis ocymifolia (Burm F.) Iwarsson leaves Asnakech Alemu A Thesis submitted to The Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science in Pharmacology Addis Ababa University Addis Ababa, Ethiopia June, 2017 X Addis Ababa University School of Graduate Studies This is to certify that the thesis prepared by Asnakech Alemu, entitled ―Evaluation of the analgesic and anti-inflammatory activities of 80% methanol extract of Leonotis ocymifolia (Burm.f.) leaves‖ and submitted in partial fulfillment of the requirements for the degree of Master of Science in Pharmacology complies with the regulations of the university and meets the accepted standards with respect to originality and quality Signed by the Examining Committee: Internal Examiner _ Signature Date _ External Examiner _ Signature Date _ Advisor Workineh Shibeshi (PhD) Signature _ Advisor Teshome Nedi (PhD) Signature _ _ Chair of Department X Date _ Date _ ABSTRACT Evaluation of the in vivo analgesic and anti-inflammatory activities of 80% methanol extract of Leonotis ocymifolia (Burm.f.) Iwarsson leaves Asnakech Alemu Addis Ababa University, 2017 Pain and inflammation are the most common health problems treated with traditional remedies which mainly comprise medicinal plants Leonotis ocymifolia is one of the medicinal plants used in folkloric medicine of Ethiopia for years to treat various pain and inflammation disorders However, the plant has not been scientifically evaluated for the claimed activities The aim of the present study was to evaluate the anti-inflammatory and analgesic activities of the 80% methanol extract of Leonotis ocymifolia leaves using rodent models The central and peripheral analgesic activity of the extract was evaluated by using Eddy‘s hot plate method and acetic acid-induced writhing, respectively The anti-inflammatory activity of the extract was evaluated by using carrageenan-induced paw edema and cotton pellet granuloma method The study was carried out in three different dose levels of extracts 100,200 and 400mg/kg orally The extract did not produce any mortality up to 2000mg/kg In the hot plate method, the extract at all doses showed a significant (p