A proteomics study of chemically induced cirrhosis in rat liver revealed the mechanism of thioacelamide hepatotoxicity 3

Chapter Chapter Results Chapter 3.1 Histo-pathology of rat liver tissues Generally, with increasing duration of TAA treatment, the fibrous content in the livers increased as compared to the controls For example, after weeks of treatment, most of the experimental rats had scores of 2s to 3s at ten different fields being analyzed (Table 3-1) as compared to the scores of 0s and 1s for the corresponding controls This shows that with only weeks of TAA treatment, the rat livers had developed increased deposition of collagen, but septa were still absent or incomplete in the liver samples (Figure 3-1) However, after weeks (Table 3-2 and Figure 3-2) and 10 weeks (Table 3-3 and Figure 3-3) of treatment, most of the ten fields examined in the treated livers had maximum scores of This means that most of the liver at this stage had developed fullblown cirrhosis, with increase of collagen and formation of complete septa, some even with obvious nodularity (marked with “n”), as observed in Figure 3-2 and 3-3 Collectively, these results show that our approach had successfully generated fibrosis and cirrhosis in the rat livers The details of the histo-pathological slides and scores are illustrated from Table 3-1 and Figure 3-1 onwards 83 Chapter Table 3-1 Histo-pathological scores at ten different fields for rats treated with TAA for weeks Rat Serial No Histo-pathological scores at ten different fields for rats after weeks of TAA treatment 0,0,1,1,0,0,0,1,0,0 SR65 1,0,1,1,1,1,0,1,1,1 SR66 0,1,0,1,1,0,0,0,1,1 SR85 0,0,1,1,0,1,1,1,0,0 SR86 0,1,1,1,0,1,1,1,0,1 SR16 EXPERIMENTAL 0,0,0,0,0,1,0,0,0,0 SR64 CONTROL SR63 3,3,3,2,3,3,2,3,3,2 SR17 3,3,3,3,3,3,3,3,3,2 SR18 3,3,3,3,3,3,3,3,3,2 SR19 2,3,2,2,3,3,3,2,3,3 SR21 2,2,2,3,2,2,1,2,2,3 SR22 4,3,3,3,4,3,3,3,2,3 84 Chapter 3 week control week experimental Figure 3-1 Tissue sections (x20) of rat livers stained with Mason Trichrome stain for rat liver tissues treated with TAA for weeks 85 Chapter Table 3-2 Histo-pathological scores at ten different fields for rats treated with TAA for weeks Rat Serial No Histo-pathological scores at ten different fields for rats after weeks of TAA treatment 1,1,1,1,1,2,1,1,1,1 SR55 0,1,0,0,0,1,0,1,0,1 SR57 0,0,1,1,1,1,0,1,0,0 SR58 0,0,0,0,1,0,0,0,0,1 SR88 1,1,1,0,1,1,1,1,1,0 SR2 EXPERIMENTAL 0,0,0,0,0,0,1,0,0,1 SR8 CONTROL SR5 4,4,4,4,4,4,4,4,4,4 SR3 4,4,4,4,3,4,4,4,4,4 SR7 4,4,4,4,4,4,4,4,4,4 SR9 4,4,4,4,4,4,4,4,4,4 SR54 4,3,4,4,4,4,4,3,4,4 SR56 4,4,4,4,4,4,4,4,4,4 86 Chapter week control week experimental n Figure 3-2 Tissue sections (x20) of rat livers stained with Mason Trichrome stain Cirrhotic nodules (n) are present at weeks experimental liver sections Fibrous tissue is stained green 87 Chapter Table 3-3 Histo-pathological scores at ten different fields for rats treated with TAA for 10 weeks Rat Serial No Histo-pathological scores at ten different fields for rats after 10 weeks of TAA treatment 1,0,0,0,0,0,0,0,0,0 SR81 1,1,0,1,1,1,0,1,0,0 SR82 0,0,0,1,0,0,0,0,0,0 SR83 0,0,1,0,0,1,0,0,0,1 SR84 0,0,1,1,0,1,0,0,0,0 SR67 EXPERIMENTAL 0,0,0,0,0,0,0,1,0,0 SR80 CONTROL SR79 4,4,4,4,4,4,4,4,4,4 SR73 4,4,3,4,4,4,4,4,4,4 SR74 4,4,4,4,4,4,4,4,4,4 SR75 4,4,4,4,4,4,4,4,4,4 SR77 4,4,4,4,4,4,4,4,4,4 SR78 4,4,4,4,4,4,4,4,4,4 88 Chapter 10 week control 10 week experimental n Figure 3-3 Tissue sections (x20) of rat livers stained with Mason Trichrome stain Cirrhotic nodules (n) are present at 10 weeks experimental liver sections Fibrous tissue is stained green 89 Chapter We performed student’s t test to confirm the differences At weeks, the mean fibrosis score of the control animals was 0.483 The corresponding score for the experimental animals was 2.7, which was significantly higher than its control (p